product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse Ly-6G
catalog :
127661
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
1A8
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments, mass cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s6a
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  • flow cytometry; mouse; fig s2
Aiken T, Erbe A, Zebertavage L, Komjathy D, Feils A, Rodriguez M, et al. Mechanism of effective combination radio-immunotherapy against 9464D-GD2, an immunologically cold murine neuroblastoma. J Immunother Cancer. 2022;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Ngamsri K, Fuhr A, Schindler K, Simelitidis M, Hagen M, Zhang Y, et al. Sevoflurane Dampens Acute Pulmonary Inflammation via the Adenosine Receptor A2B and Heme Oxygenase-1. Cells. 2022;11: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a, 4c
Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
  • flow cytometry; mouse; 1:150; loading ...; fig s2
Mach xe1 x10d ek T, Leontovy x10d R, x160 m xed dov xe1 B, Majer M, Vondr xe1 x10d ek O, Vojt x11b chov xe1 I, et al. Mechanisms of the host immune response and helminth-induced pathology during Trichobilharzia regenti (Schistosomatidae) neuroinvasion in mice. PLoS Pathog. 2022;18:e1010302 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Vaillant L, Oster P, McMillan B, Orozco Fernandez E, Velin D. GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Helicobacter. 2022;27:e12875 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig 7
Chakraborty R, Maltz M, Del Castillo D, Tandel P, Messih N, Anguiano M, et al. Selective Targeting of Tumour Necrosis Factor Receptor 1 Induces Stable Protection from Crohn's-Like Ileitis in TNFΔARE Mice. J Crohns Colitis. 2022;16:978-991 pubmed publisher
  • flow cytometry; human; fig s3a
Fahy N, Palomares Cabeza V, Lolli A, Witte Bouma J, Merino A, Ridwan Y, et al. Chondrogenically Primed Human Mesenchymal Stem Cells Persist and Undergo Early Stages of Endochondral Ossification in an Immunocompetent Xenogeneic Model. Front Immunol. 2021;12:715267 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Gurley J, Gmyrek G, Hargis E, Bishop G, Carr D, Elliott M. The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation. Cells. 2021;10: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s2a
Otto N, Pereverzeva L, Léopold V, Ramirez Moral I, Roelofs J, van Heijst J, et al. Hypoxia-Inducible Factor-1α in Macrophages, but Not in Neutrophils, Is Important for Host Defense during Klebsiella pneumoniae-Induced Pneumosepsis. Mediators Inflamm. 2021;2021:9958281 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 5b, 5d
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; fig 3
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; loading ...
Mathä L, Romera Hernandez M, Steer C, Yin Y, Orangi M, Shim H, et al. Migration of Lung Resident Group 2 Innate Lymphoid Cells Link Allergic Lung Inflammation and Liver Immunity. Front Immunol. 2021;12:679509 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Petermann M, Orfanos Z, Sellau J, Gharaibeh M, Lotter H, Fleischer B, et al. CCR2 Deficiency Impairs Ly6Clo and Ly6Chi Monocyte Responses in Orientia tsutsugamushi Infection. Front Immunol. 2021;12:670219 pubmed publisher
  • flow cytometry; mouse
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; fig 5a
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; loading ...
Geng G, Liu J, Xu C, Pei Y, Chen L, Mu C, et al. Receptor-mediated mitophagy regulates EPO production and protects against renal anemia. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5m
Piñeiro Hermida S, Martinez P, Blasco M. Short and dysfunctional telomeres protect from allergen-induced airway inflammation. Aging Cell. 2021;20:e13352 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Go D, Lee S, Lee S, Woo S, Kim K, Kim K, et al. Programmed Death Ligand 1-Expressing Classical Dendritic Cells MitigateHelicobacter-Induced Gastritis. Cell Mol Gastroenterol Hepatol. 2021;12:715-739 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 6a
Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  • flow cytometry; human; 1:250; loading ...; fig s9
Qin W, Brands X, Van T Veer C, F de Vos A, Sirard J, J T H Roelofs J, et al. Bronchial epithelial DNA methyltransferase 3b dampens pulmonary immune responses during Pseudomonas aeruginosa infection. PLoS Pathog. 2021;17:e1009491 pubmed publisher
  • flow cytometry; mouse; loading ...
Ngamsri K, Gamper Tsigaras J, Reutershan J, Konrad F. Fractalkine Is Linked to the Necrosome Pathway in Acute Pulmonary Inflammation. Front Med (Lausanne). 2021;8:591790 pubmed publisher
  • flow cytometry; mouse; fig 5b
Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 2
Steenbrugge J, Vander Elst N, Demeyere K, De Wever O, Sanders N, van den Broeck W, et al. OMO-1 reduces progression and enhances cisplatin efficacy in a 4T1-based non-c-MET addicted intraductal mouse model for triple-negative breast cancer. NPJ Breast Cancer. 2021;7:27 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s1b, s1c, s1d
  • immunohistochemistry; mouse; 1:500; loading ...; fig 4d
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; fig s1c, s4d
Can xe8 S, Van Snick J, Uyttenhove C, Pilotte L, van den Eynde B. TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. J Immunother Cancer. 2021;9: pubmed publisher
  • blocking or activating experiments; mouse; fig 4c
Wang X, Liu D, Qin W, Liu Y, Yuan X, Zhang X, et al. P2RX1-Involved Glycolytic Metabolism Supports Neutrophil Activation in Acute Pancreatitis. Front Immunol. 2020;11:549179 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Yoon S, Song S, Shin J, Kang S, Kim H, You H. Protective Effects of Korean Herbal Remedy against Airway Inflammation in an Allergic Asthma by Suppressing Eosinophil Recruitment and Infiltration in Lung. Antioxidants (Basel). 2020;10: pubmed publisher
  • flow cytometry; mouse; 1:250; loading ...; fig s8a
Torretta S, Scagliola A, Ricci L, Mainini F, Di Marco S, Cuccovillo I, et al. D-mannose suppresses macrophage IL-1β production. Nat Commun. 2020;11:6343 pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...
Jinno A, Hayashida A, Jenkinson H, Park P. Syndecan-1 Promotes Streptococcus pneumoniae Corneal Infection by Facilitating the Assembly of Adhesive Fibronectin Fibrils. MBio. 2020;11: pubmed publisher
  • flow cytometry; mouse; loading ...
Myers D, Abram C, Wildes D, Belwafa A, Welsh A, Schulze C, et al. Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity. Front Immunol. 2020;11:576310 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6
Gurley J, Gmyrek G, McClellan M, Hargis E, Hauck S, Dozmorov M, et al. Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina. Invest Ophthalmol Vis Sci. 2020;61:19 pubmed publisher
  • flow cytometry; mouse; loading ...
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Shi H, Lo T, Ma D, Condor B, Lesmana B, Parungao R, et al. Dihydrotestosterone (DHT) Enhances Wound Healing of Major Burn Injury by Accelerating Resolution of Inflammation in Mice. Int J Mol Sci. 2020;21: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Li N, Kang Y, Wang L, Huff S, Tang R, Hui H, et al. ALKBH5 regulates anti-PD-1 therapy response by modulating lactate and suppressive immune cell accumulation in tumor microenvironment. Proc Natl Acad Sci U S A. 2020;117:20159-20170 pubmed publisher
  • flow cytometry; mouse; 1:200; fig s3
Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
  • flow cytometry; mouse; loading ...
Ngamsri K, Jans C, Putri R, Schindler K, Gamper Tsigaras J, Eggstein C, et al. Inhibition of CXCR4 and CXCR7 Is Protective in Acute Peritoneal Inflammation. Front Immunol. 2020;11:407 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Watanabe M, Kaneko Y, Ohishi Y, Kinoshita M, Sakairi T, Ikeuchi H, et al. Importance of methodology in the evaluation of renal mononuclear phagocytes and analysis of a model of experimental nephritis with Shp1 conditional knockout mice. Biochem Biophys Rep. 2020;22:100741 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Wilson Z, Witt H, Hazlett L, Harman M, Neumann B, Whitman A, et al. Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking. Sci Rep. 2020;10:2142 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 1.5 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
Banka A, Guevara M, Brannon E, Nguyen N, Song S, Cady G, et al. Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation. Nat Commun. 2023;14:2462 pubmed publisher
Haffner Luntzer M, Weber B, Morioka K, Lackner I, Fischer V, Bahney C, et al. Altered early immune response after fracture and traumatic brain injury. Front Immunol. 2023;14:1074207 pubmed publisher
Pernet E, Sun S, Sarden N, Gona S, Nguyen A, Khan N, et al. Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE. Nature. 2023;614:530-538 pubmed publisher
Li Y, Xu J, Yu T, Zhu J, Xuan A, Liu X, et al. A labeling strategy for the three-dimensional recognition and analysis of microvascular obstruction in ischemic stroke. Theranostics. 2023;13:403-416 pubmed publisher
Kuhn M, Haffner Luntzer M, Kempter E, Reber S, Ichinose H, Vacher J, et al. Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice. Front Endocrinol (Lausanne). 2022;13:997745 pubmed publisher
Gawish R, Maier B, Obermayer G, Watzenboeck M, Gorki A, Quattrone F, et al. A neutrophil-B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4. elife. 2022;11: pubmed publisher
Hu J, Ji Q, Chen F, Gong X, Chen C, Zhang K, et al. CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration. J Immunol Res. 2022;2022:7966089 pubmed publisher
Fischer V, Ragipoglu D, Diedrich J, Steppe L, Dudeck A, Sch xfc tze K, et al. Mast Cells Trigger Disturbed Bone Healing in Osteoporotic Mice. J Bone Miner Res. 2022;37:137-151 pubmed publisher
Kim A, Kim S, Leszczyńska A, Kaufmann B, Reca A, Kim D, et al. Dual role of neutrophils in modulating liver injury and fibrosis during development and resolution of diet-induced murine steatohepatitis. Sci Rep. 2021;11:24194 pubmed publisher
Zheng Z, Li Y, Jia S, Zhu M, Cao L, Tao M, et al. Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3. Nat Commun. 2021;12:6202 pubmed publisher
Kaiser R, Leunig A, Pekayvaz K, Popp O, Joppich M, Polewka V, et al. Self-sustaining IL-8 loops drive a prothrombotic neutrophil phenotype in severe COVID-19. JCI Insight. 2021;6: pubmed publisher
Deng H, Kan A, Lyu N, He M, Huang X, Qiao S, et al. Tumor-derived lactate inhibit the efficacy of lenvatinib through regulating PD-L1 expression on neutrophil in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
Pan Y, Nishikawa T, Chang C, Tai J, Kaneda Y. CXCL2 combined with HVJ-E suppresses tumor growth and lung metastasis in breast cancer and enhances anti-PD-1 antibody therapy. Mol Ther Oncolytics. 2021;20:175-186 pubmed publisher
Kalafati L, Kourtzelis I, Schulte Schrepping J, Li X, Hatzioannou A, Grinenko T, et al. Innate Immune Training of Granulopoiesis Promotes Anti-tumor Activity. Cell. 2020;183:771-785.e12 pubmed publisher
Chen J, Zhu Z, Li Q, Lin Y, Dang E, Meng H, et al. Neutrophils Enhance Cutaneous Vascular Dilation and Permeability to Aggravate Psoriasis by Releasing Matrix Metallopeptidase 9. J Invest Dermatol. 2021;141:787-799 pubmed publisher
Shang Q, Chu Y, Li Y, Han Y, Yu D, Liu R, et al. Adipose-derived mesenchymal stromal cells promote corneal wound healing by accelerating the clearance of neutrophils in cornea. Cell Death Dis. 2020;11:707 pubmed publisher
Calvente C, Tameda M, Johnson C, Del Pilar H, Lin Y, Adronikou N, et al. Neutrophils contribute to spontaneous resolution of liver inflammation and fibrosis via microRNA-223. J Clin Invest. 2019;129:4091-4109 pubmed publisher
Haffner Luntzer M, Foertsch S, Fischer V, Prystaz K, Tschaffon M, Mödinger Y, et al. Chronic psychosocial stress compromises the immune response and endochondral ossification during bone fracture healing via β-AR signaling. Proc Natl Acad Sci U S A. 2019;116:8615-8622 pubmed publisher
Liu Y, Teng Y, Cheng P, Kong H, Lv Y, Mao F, et al. Abrogation of cathepsin C by Helicobacter pylori impairs neutrophil activation to promote gastric infection. FASEB J. 2019;33:5018-5033 pubmed publisher
Mödinger Y, Rapp A, Vikman A, Ren Z, Fischer V, Bergdolt S, et al. Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing. Am J Pathol. 2019;189:147-161 pubmed publisher
Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed publisher
Kaiser K, Prystaz K, Vikman A, Haffner Luntzer M, Bergdolt S, Strauss G, et al. Pharmacological inhibition of IL-6 trans-signaling improves compromised fracture healing after severe trauma. Naunyn Schmiedebergs Arch Pharmacol. 2018;391:523-536 pubmed publisher
Prystaz K, Kaiser K, Kovtun A, Haffner Luntzer M, Fischer V, Rapp A, et al. Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing. Am J Pathol. 2018;188:474-490 pubmed publisher
Bergdolt S, Kovtun A, Hägele Y, Liedert A, Schinke T, Amling M, et al. Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing. PLoS ONE. 2017;12:e0179512 pubmed publisher
Kemmler J, Bindl R, McCook O, Wagner F, Gröger M, Wagner K, et al. Exposure to 100% Oxygen Abolishes the Impairment of Fracture Healing after Thoracic Trauma. PLoS ONE. 2015;10:e0131194 pubmed publisher
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC, Depletion, IHC
Cat # :
127661
Clone :
1A8
Item :
Ultra-LEAF™ Purified anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA