product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 785™ anti-mouse Ly-6G
catalog :
127645
quantity :
50 μg
price :
297 USD
clonality :
monoclonal
host :
rat
conjugate :
Brilliant Violet 785
clone name :
1A8
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments, other
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 1e
Kaur S, Sehgal A, Wu A, Millard S, Batoon L, Sandrock C, et al. Stable colony-stimulating factor 1 fusion protein treatment increases hematopoietic stem cell pool and enhances their mobilisation in mice. J Hematol Oncol. 2021;14:3 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s5
Deng M, Tam J, Wang L, Liang K, Li S, Zhang L, et al. TRAF3IP3 negatively regulates cytosolic RNA induced anti-viral signaling by promoting TBK1 K48 ubiquitination. Nat Commun. 2020;11:2193 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7s1a
Hill R, Hoffman B, Morita T, Campos S, Lumpkin E, Brem R, et al. The signaling lipid sphingosine 1-phosphate regulates mechanical pain. elife. 2018;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Klein J, Moses K, Zelinskyy G, Sody S, Buer J, Lang S, et al. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth. Nat Commun. 2017;8:14600 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Ramos G, van den Berg A, Nunes Silva V, Weirather J, Peters L, Burkard M, et al. Myocardial aging as a T-cell-mediated phenomenon. Proc Natl Acad Sci U S A. 2017;114:E2420-E2429 pubmed publisher
  • flow cytometry; mouse; fig 5
Huang A, Peng D, Guo H, Ben Y, Zuo X, Wu F, et al. A human programmed death-ligand 1-expressing mouse tumor model for evaluating the therapeutic efficacy of anti-human PD-L1 antibodies. Sci Rep. 2017;7:42687 pubmed publisher
  • flow cytometry; mouse; loading ...; tbl 2
Ellenbroek G, van Puijvelde G, Anas A, Bot M, Asbach M, Schoneveld A, et al. Leukocyte TLR5 deficiency inhibits atherosclerosis by reduced macrophage recruitment and defective T-cell responsiveness. Sci Rep. 2017;7:42688 pubmed publisher
  • flow cytometry; mouse; fig 1b
Gardner P, Liyanage S, Cristante E, Sampson R, Dick A, Ali R, et al. Hypoxia inducible factors are dispensable for myeloid cell migration into the inflamed mouse eye. Sci Rep. 2017;7:40830 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3b
Fujikura D, Ikesue M, Endo T, Chiba S, Higashi H, Uede T. Death receptor 6 contributes to autoimmunity in lupus-prone mice. Nat Commun. 2017;8:13957 pubmed publisher
  • other; mouse; loading ...; fig 3a
Kamioka Y, Takakura K, Sumiyama K, Matsuda M. Intravital Förster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli. Cancer Sci. 2017;108:226-235 pubmed publisher
  • flow cytometry; mouse; 1:200; fig s1a
Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest. 2017;127:383-401 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Swanson P, Hart G, Russo M, Nayak D, Yazew T, Pena M, et al. CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature. PLoS Pathog. 2016;12:e1006022 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Kirschbaum K, Sonner J, Zeller M, Deumelandt K, Bode J, Sharma R, et al. In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis. Proc Natl Acad Sci U S A. 2016;113:13227-13232 pubmed
  • flow cytometry; mouse; loading ...; fig 6a
Yu S, Pearson A, Lim R, Rodgers D, Li S, Parker H, et al. Targeted Delivery of an Anti-inflammatory PDE4 Inhibitor to Immune Cells via an Antibody-drug Conjugate. Mol Ther. 2016;24:2078-2089 pubmed publisher
  • flow cytometry; mouse; 1:200; tbl 1
Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Papp S, Moderzynski K, Rauch J, Heine L, Kuehl S, Richardt U, et al. Liver Necrosis and Lethal Systemic Inflammation in a Murine Model of Rickettsia typhi Infection: Role of Neutrophils, Macrophages and NK Cells. PLoS Negl Trop Dis. 2016;10:e0004935 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Barin J, Talor M, Schaub J, Diny N, Hou X, Hoyer M, et al. Collaborative Interferon-? and Interleukin-17 Signaling Protects the Oral Mucosa from Staphylococcus aureus. Am J Pathol. 2016;186:2337-52 pubmed publisher
  • flow cytometry; mouse; fig 2
Eichenfield D, Troutman T, Link V, Lam M, Cho H, Gosselin D, et al. Tissue damage drives co-localization of NF-?B, Smad3, and Nrf2 to direct Rev-erb sensitive wound repair in mouse macrophages. elife. 2016;5: pubmed publisher
  • flow cytometry; mouse; fig s1
Fransén Pettersson N, Duarte N, Nilsson J, Lundholm M, Mayans S, Larefalk A, et al. A New Mouse Model That Spontaneously Develops Chronic Liver Inflammation and Fibrosis. PLoS ONE. 2016;11:e0159850 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6b
Xiao Y, Tang J, Guo H, Zhao Y, Tang R, Ouyang S, et al. Targeting CBLB as a potential therapeutic approach for disseminated candidiasis. Nat Med. 2016;22:906-14 pubmed publisher
  • immunohistochemistry; mouse; 2.5 ug/ml; loading ...; fig 5f
Kretschmer S, Pieper M, Hüttmann G, Bölke T, Wollenberg B, Marsh L, et al. Autofluorescence multiphoton microscopy for visualization of tissue morphology and cellular dynamics in murine and human airways. Lab Invest. 2016;96:918-31 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 3a
Ruhland M, Loza A, Capietto A, Luo X, Knolhoff B, Flanagan K, et al. Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis. Nat Commun. 2016;7:11762 pubmed publisher
  • immunohistochemistry; mouse; 1:150
Chen W, Cao Z, Sugaya S, Lopez M, Sendra V, Laver N, et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun. 2016;7:11302 pubmed publisher
  • flow cytometry; mouse; fig S7
Peteranderl C, Morales Nebreda L, Selvakumar B, Lecuona E, Vadász I, Morty R, et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest. 2016;126:1566-80 pubmed publisher
  • blocking or activating experiments; mouse; fig 8
Kocijancic D, Felgner S, Frahm M, Komoll R, Iljazovic A, Pawar V, et al. Therapy of solid tumors using probiotic Symbioflor-2: restraints and potential. Oncotarget. 2016;7:22605-22 pubmed publisher
  • flow cytometry; mouse; loading ...
Smith R, Reyes N, Khandelwal P, Schlereth S, Lee H, Masli S, et al. Secondary allergic T cell responses are regulated by dendritic cell-derived thrombospondin-1 in the setting of allergic eye disease. J Leukoc Biol. 2016;100:371-80 pubmed publisher
  • flow cytometry; mouse
Azpilikueta A, Agorreta J, Labiano S, Pérez Gracia J, Sánchez Paulete A, Aznar M, et al. Successful Immunotherapy against a Transplantable Mouse Squamous Lung Carcinoma with Anti-PD-1 and Anti-CD137 Monoclonal Antibodies. J Thorac Oncol. 2016;11:524-36 pubmed publisher
  • flow cytometry; mouse; fig 2
Montufar Solis D, Klein J. Splenic Leukocytes Traffic to the Thyroid and Produce a Novel TSHβ Isoform during Acute Listeria monocytogenes Infection in Mice. PLoS ONE. 2016;11:e0146111 pubmed publisher
  • flow cytometry; mouse; fig s5
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse; fig 6
Gallego Ortega D, Ledger A, Roden D, Law A, Magenau A, Kikhtyak Z, et al. ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells. PLoS Biol. 2015;13:e1002330 pubmed publisher
  • flow cytometry; mouse; fig 2
Kim J, Phan T, Nguyen V, Dinh Vu H, Zheng J, Yun M, et al. Salmonella typhimurium Suppresses Tumor Growth via the Pro-Inflammatory Cytokine Interleukin-1β. Theranostics. 2015;5:1328-42 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Vlachou K, Mintzas K, Glymenaki M, Ioannou M, Papadaki G, Bertsias G, et al. Elimination of Granulocytic Myeloid-Derived Suppressor Cells in Lupus-Prone Mice Linked to Reactive Oxygen Species-Dependent Extracellular Trap Formation. Arthritis Rheumatol. 2016;68:449-61 pubmed publisher
  • flow cytometry; mouse; fig 1
Fujimura N, Xu B, Dalman J, Deng H, Aoyama K, Dalman R. CCR2 inhibition sequesters multiple subsets of leukocytes in the bone marrow. Sci Rep. 2015;5:11664 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Herz J, Johnson K, McGavern D. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. J Exp Med. 2015;212:1153-69 pubmed publisher
  • flow cytometry; mouse; fig 1
Conde P, Rodriguez M, van der Touw W, Jimenez A, Burns M, Miller J, et al. DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance. Immunity. 2015;42:1143-58 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 8
Chang D, Moniz R, Xu Z, Sun J, Signoretti S, Zhu Q, et al. Human anti-CAIX antibodies mediate immune cell inhibition of renal cell carcinoma in vitro and in a humanized mouse model in vivo. Mol Cancer. 2015;14:119 pubmed publisher
  • blocking or activating experiments; mouse; fig 2
Durrans A, Gao D, Gupta R, Fischer K, Choi H, El Rayes T, et al. Identification of Reprogrammed Myeloid Cell Transcriptomes in NSCLC. PLoS ONE. 2015;10:e0129123 pubmed publisher
  • flow cytometry; mouse; 1:200; fig s2
Castiglioni A, Corna G, Rigamonti E, Basso V, Vezzoli M, Monno A, et al. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration. PLoS ONE. 2015;10:e0128094 pubmed publisher
  • flow cytometry; mouse; fig 5
Hegde V, Singh U, Nagarkatti P, Nagarkatti M. Critical Role of Mast Cells and Peroxisome Proliferator-Activated Receptor γ in the Induction of Myeloid-Derived Suppressor Cells by Marijuana Cannabidiol In Vivo. J Immunol. 2015;194:5211-22 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3
Brunner P, Glitzner E, Reininger B, Klein I, Stary G, Mildner M, et al. CCL7 contributes to the TNF-alpha-dependent inflammation of lesional psoriatic skin. Exp Dermatol. 2015;24:522-8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
  • flow cytometry; mouse; fig 4
Baillet A, Rehaume L, Benham H, O Meara C, Armitage C, Ruscher R, et al. High Chlamydia Burden Promotes Tumor Necrosis Factor-Dependent Reactive Arthritis in SKG Mice. Arthritis Rheumatol. 2015;67:1535-47 pubmed publisher
  • flow cytometry; mouse; fig s1
Evrard M, Chong S, Devi S, Chew W, Lee B, Poidinger M, et al. Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy. J Leukoc Biol. 2015;97:611-9 pubmed publisher
  • flow cytometry; mouse
Fontana M, Baccarella A, Pancholi N, Pufall M, Herbert D, Kim C. JUNB is a key transcriptional modulator of macrophage activation. J Immunol. 2015;194:177-86 pubmed publisher
  • flow cytometry; mouse; fig 4
Lieber S, Scheer F, Finkernagel F, Meissner W, Giehl G, Brendel C, et al. The inverse agonist DG172 triggers a PPARβ/δ-independent myeloid lineage shift and promotes GM-CSF/IL-4-induced dendritic cell differentiation. Mol Pharmacol. 2015;87:162-73 pubmed publisher
  • flow cytometry; mouse; fig 5
Morales D, Monte K, Sun L, Struckhoff J, Agapov E, Holtzman M, et al. Novel mode of ISG15-mediated protection against influenza A virus and Sendai virus in mice. J Virol. 2015;89:337-49 pubmed publisher
  • flow cytometry; mouse; fig 3a
Stoilova B, Kowenz Leutz E, Scheller M, Leutz A. Lymphoid to myeloid cell trans-differentiation is determined by C/EBP? structure and post-translational modifications. PLoS ONE. 2013;8:e65169 pubmed publisher
Chakraborty S, González J, Sievers B, Mallajosyula V, Chakraborty S, Dubey M, et al. Early non-neutralizing, afucosylated antibody responses are associated with COVID-19 severity. Sci Transl Med. 2022;14:eabm7853 pubmed publisher
Dewi I, Cunha C, Jaeger M, Gresnigt M, Gkountzinopoulou M, Garishah F, et al. Neuraminidase and SIGLEC15 modulate the host defense against pulmonary aspergillosis. Cell Rep Med. 2021;2:100289 pubmed publisher
Brestoff J, Wilen C, Moley J, Li Y, Zou W, Malvin N, et al. Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Cell Metab. 2021;33:270-282.e8 pubmed publisher
Mogilenko D, Shpynov O, Andhey P, Arthur L, Swain A, Esaulova E, et al. Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK+ CD8+ T Cells as Conserved Hallmark of Inflammaging. Immunity. 2021;54:99-115.e12 pubmed publisher
Ruhland M, Roberts E, Cai E, Mujal A, Marchuk K, Beppler C, et al. Visualizing Synaptic Transfer of Tumor Antigens among Dendritic Cells. Cancer Cell. 2020;37:786-799.e5 pubmed publisher
Devos M, Tanghe G, Gilbert B, Dierick E, Verheirstraeten M, Nemegeer J, et al. Sensing of endogenous nucleic acids by ZBP1 induces keratinocyte necroptosis and skin inflammation. J Exp Med. 2020;217: pubmed publisher
Forero A, Ozarkar S, Li H, Lee C, Hemann E, Nadjsombati M, et al. Differential Activation of the Transcription Factor IRF1 Underlies the Distinct Immune Responses Elicited by Type I and Type III Interferons. Immunity. 2019;51:451-464.e6 pubmed publisher
Huggins M, Sjaastad F, Pierson M, Kucaba T, Swanson W, Staley C, et al. Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell Rep. 2019;28:1729-1743.e5 pubmed publisher
Hou X, Chen G, Bracamonte Baran W, Choi H, Diny N, Sung J, et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep. 2019;28:172-189.e7 pubmed publisher
Barry K, Hsu J, Broz M, Cueto F, Binnewies M, Combes A, et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med. 2018;24:1178-1191 pubmed publisher
Her Z, Teng T, Tan J, Teo T, Kam Y, Lum F, et al. Loss of TLR3 aggravates CHIKV replication and pathology due to an altered virus-specific neutralizing antibody response. EMBO Mol Med. 2015;7:24-41 pubmed publisher
McCartney Francis N, Jin W, Belkaid Y, McGrady G, Wahl S. Aberrant host defense against Leishmania major in the absence of SLPI. J Leukoc Biol. 2014;96:917-29 pubmed publisher
Ng L, Qin J, Roediger B, Wang Y, Jain R, Cavanagh L, et al. Visualizing the neutrophil response to sterile tissue injury in mouse dermis reveals a three-phase cascade of events. J Invest Dermatol. 2011;131:2058-68 pubmed publisher
Fujita M, Kohanbash G, Fellows Mayle W, Hamilton R, Komohara Y, Decker S, et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res. 2011;71:2664-74 pubmed publisher
Guiducci C, Tripodo C, Gong M, Sangaletti S, Colombo M, Coffman R, et al. Autoimmune skin inflammation is dependent on plasmacytoid dendritic cell activation by nucleic acids via TLR7 and TLR9. J Exp Med. 2010;207:2931-42 pubmed publisher
Sumagin R, Prizant H, Lomakina E, Waugh R, Sarelius I. LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ. J Immunol. 2010;185:7057-66 pubmed publisher
Tadagavadi R, REEVES W. Endogenous IL-10 attenuates cisplatin nephrotoxicity: role of dendritic cells. J Immunol. 2010;185:4904-11 pubmed publisher
Daley J, Thomay A, Connolly M, Reichner J, Albina J. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol. 2008;83:64-70 pubmed
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC
Cat # :
127645
Clone :
1A8
Item :
Brilliant Violet 785™ anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
50 μg
Price (USD) :
297 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV785
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA