product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse Ly-6G
catalog :
127627
quantity :
125 μL
price :
171 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
1A8
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig ex3a
Culemann S, Grüneboom A, Nicolás Ávila J, Weidner D, Lämmle K, Rothe T, et al. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature. 2019;572:670-675 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; mouse; fig 1b
Gardner P, Liyanage S, Cristante E, Sampson R, Dick A, Ali R, et al. Hypoxia inducible factors are dispensable for myeloid cell migration into the inflamed mouse eye. Sci Rep. 2017;7:40830 pubmed publisher
  • flow cytometry; mouse; 1:200; tbl 1
Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
  • flow cytometry; mouse; fig s2
Ermert D, Shaughnessy J, Joeris T, Kaplan J, Pang C, Kurt Jones E, et al. Virulence of Group A Streptococci Is Enhanced by Human Complement Inhibitors. PLoS Pathog. 2015;11:e1005043 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
Khamissi F, Ning L, Kefaloyianni E, Dun H, Arthanarisami A, Keller A, et al. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure. Sci Adv. 2022;8:eabm5900 pubmed publisher
Wang L, Chiu S, Lee W, Siu L, Liu K, Yen M, et al. Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella. STAR Protoc. 2021;2:100337 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Rustenhoven J, Drieu A, Mamuladze T, de Lima K, Dykstra T, Wall M, et al. Functional characterization of the dural sinuses as a neuroimmune interface. Cell. 2021;184:1000-1016.e27 pubmed publisher
Murakami K, Kurotaki D, Kawase W, Soma S, Fukuchi Y, Kunimoto H, et al. OGT Regulates Hematopoietic Stem Cell Maintenance via PINK1-Dependent Mitophagy. Cell Rep. 2021;34:108579 pubmed publisher
Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards S, et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel). 2020;12: pubmed publisher
Wang L, Wang H, Chiang H, Huang L, Chiu S, Siu L, et al. Human Placental MSC-Secreted IL-1β Enhances Neutrophil Bactericidal Functions during Hypervirulent Klebsiella Infection. Cell Rep. 2020;32:108188 pubmed publisher
Hasegawa T, Kikuta J, Sudo T, Yamashita E, Seno S, Takeuchi T, et al. Development of an intravital imaging system for the synovial tissue reveals the dynamics of CTLA-4 Ig in vivo. Sci Rep. 2020;10:13480 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Jackson Jones L, Smith P, Portman J, Magalhaes M, Mylonas K, Vermeren M, et al. Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants. Immunity. 2020;52:700-715.e6 pubmed publisher
Kohler D, Granja T, Volz J, Koeppen M, Langer H, Hansmann G, et al. Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb. Nat Commun. 2020;11:1315 pubmed publisher
Pereira J, Gerber J, Ghidinelli M, Gerber D, Tortola L, Ommer A, et al. Mice carrying an analogous heterozygous dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy. Hum Mol Genet. 2020;29:1253-1273 pubmed publisher
Muri J, Thut H, Feng Q, Kopf M. Thioredoxin-1 distinctly promotes NF-κB target DNA binding and NLRP3 inflammasome activation independently of Txnip. elife. 2020;9: pubmed publisher
Wang L, Li Y, Wang X, Wang P, Essandoh K, Cui S, et al. GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype. Cells. 2020;9: pubmed publisher
Mansouri N, Willis G, Fernandez Gonzalez A, Reis M, Nassiri S, Mitsialis S, et al. Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes. JCI Insight. 2019;4: pubmed publisher
Nepal S, Tiruppathi C, Tsukasaki Y, Farahany J, Mittal M, Rehman J, et al. STAT6 induces expression of Gas6 in macrophages to clear apoptotic neutrophils and resolve inflammation. Proc Natl Acad Sci U S A. 2019;116:16513-16518 pubmed publisher
Pundir P, Liu R, Vasavda C, Serhan N, Limjunyawong N, Yee R, et al. A Connective Tissue Mast-Cell-Specific Receptor Detects Bacterial Quorum-Sensing Molecules and Mediates Antibacterial Immunity. Cell Host Microbe. 2019;26:114-122.e8 pubmed publisher
Fleskens V, Minutti C, Wu X, Wei P, Pals C, McCrae J, et al. Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells. Cell Rep. 2019;26:3600-3612.e6 pubmed publisher
Barman P, Pang J, Urao N, Koh T. Skin Wounding-Induced Monocyte Expansion in Mice Is Not Abrogated by IL-1 Receptor 1 Deficiency. J Immunol. 2019;202:2720-2727 pubmed publisher
Angelidis I, Simon L, Fernandez I, Strunz M, Mayr C, Greiffo F, et al. An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics. Nat Commun. 2019;10:963 pubmed publisher
Minutti C, Modak R, MacDonald F, Li F, Smyth D, Dorward D, et al. A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation. Immunity. 2019;50:645-654.e6 pubmed publisher
Green D, Limjunyawong N, Gour N, Pundir P, Dong X. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron. 2019;101:412-420.e3 pubmed publisher
Fransén Pettersson N, Deronic A, Nilsson J, Hannibal T, Hansen L, Schmidt Christensen A, et al. The immunomodulatory quinoline-3-carboxamide paquinimod reverses established fibrosis in a novel mouse model for liver fibrosis. PLoS ONE. 2018;13:e0203228 pubmed publisher
Baudoux T, Husson C, De Prez E, Jadot I, Antoine M, Nortier J, et al. CD4+ and CD8+ T Cells Exert Regulatory Properties During Experimental Acute Aristolochic Acid Nephropathy. Sci Rep. 2018;8:5334 pubmed publisher
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
Linehan J, Harrison O, Han S, Byrd A, Vujkovic Cvijin I, Villarino A, et al. Non-classical Immunity Controls Microbiota Impact on Skin Immunity and Tissue Repair. Cell. 2018;172:784-796.e18 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Liyanage S, Gardner P, Ribeiro J, Cristante E, Sampson R, Luhmann U, et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res. 2016;151:160-70 pubmed publisher
Kostadinova E, Chaput C, Gutbier B, Lippmann J, Sander L, Mitchell T, et al. NLRP3 protects alveolar barrier integrity by an inflammasome-independent increase of epithelial cell adherence. Sci Rep. 2016;6:30943 pubmed publisher
Kullberg M, Martinson H, Mann K, Anchordoquy T. Complement C3 mediated targeting of liposomes to granulocytic myeloid derived suppressor cells. Nanomedicine. 2015;11:1355-63 pubmed publisher
Her Z, Teng T, Tan J, Teo T, Kam Y, Lum F, et al. Loss of TLR3 aggravates CHIKV replication and pathology due to an altered virus-specific neutralizing antibody response. EMBO Mol Med. 2015;7:24-41 pubmed publisher
Kennedy J, Fodil N, Torre S, Bongfen S, Olivier J, Leung V, et al. CCDC88B is a novel regulator of maturation and effector functions of T cells during pathological inflammation. J Exp Med. 2014;211:2519-35 pubmed publisher
Bertuzzi M, Schrettl M, Alcazar Fuoli L, Cairns T, Muñoz A, Walker L, et al. The pH-responsive PacC transcription factor of Aspergillus fumigatus governs epithelial entry and tissue invasion during pulmonary aspergillosis. PLoS Pathog. 2014;10:e1004413 pubmed publisher
McCartney Francis N, Jin W, Belkaid Y, McGrady G, Wahl S. Aberrant host defense against Leishmania major in the absence of SLPI. J Leukoc Biol. 2014;96:917-29 pubmed publisher
Radovanovic I, Leung V, Iliescu A, Bongfen S, Mullick A, Langlais D, et al. Genetic control of susceptibility to Candida albicans in SM/J mice. J Immunol. 2014;193:1290-300 pubmed publisher
Ng L, Qin J, Roediger B, Wang Y, Jain R, Cavanagh L, et al. Visualizing the neutrophil response to sterile tissue injury in mouse dermis reveals a three-phase cascade of events. J Invest Dermatol. 2011;131:2058-68 pubmed publisher
Fujita M, Kohanbash G, Fellows Mayle W, Hamilton R, Komohara Y, Decker S, et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res. 2011;71:2664-74 pubmed publisher
Guiducci C, Tripodo C, Gong M, Sangaletti S, Colombo M, Coffman R, et al. Autoimmune skin inflammation is dependent on plasmacytoid dendritic cell activation by nucleic acids via TLR7 and TLR9. J Exp Med. 2010;207:2931-42 pubmed publisher
Sumagin R, Prizant H, Lomakina E, Waugh R, Sarelius I. LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ. J Immunol. 2010;185:7057-66 pubmed publisher
Tadagavadi R, REEVES W. Endogenous IL-10 attenuates cisplatin nephrotoxicity: role of dendritic cells. J Immunol. 2010;185:4904-11 pubmed publisher
Daley J, Thomay A, Connolly M, Reichner J, Albina J. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol. 2008;83:64-70 pubmed
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC, IHC-F
Cat # :
127627
Clone :
1A8
Item :
Brilliant Violet 421™ anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
125 μL
Price (USD) :
171 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA