product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-mouse Ly-6G
catalog :
127615
quantity :
25 μg
price :
112 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP/Cy5.5
clone name :
1A8
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 89
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 6c
Gurley J, Gmyrek G, Hargis E, Bishop G, Carr D, Elliott M. The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation. Cells. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 5b, 5d
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; fig 3
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; fig 3a
Petermann M, Orfanos Z, Sellau J, Gharaibeh M, Lotter H, Fleischer B, et al. CCR2 Deficiency Impairs Ly6Clo and Ly6Chi Monocyte Responses in Orientia tsutsugamushi Infection. Front Immunol. 2021;12:670219 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5m
Piñeiro Hermida S, Martinez P, Blasco M. Short and dysfunctional telomeres protect from allergen-induced airway inflammation. Aging Cell. 2021;20:e13352 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Sugita J, Fujiu K, Nakayama Y, Matsubara T, Matsuda J, Oshima T, et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun. 2021;12:1910 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Meyer I, Goetzke C, Kespohl M, Sauter M, Heuser A, Eckstein V, et al. Silencing the CSF-1 Axis Using Nanoparticle Encapsulated siRNA Mitigates Viral and Autoimmune Myocarditis. Front Immunol. 2018;9:2303 pubmed publisher
  • flow cytometry; mouse; 1:20; loading ...; fig 3c
Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, et al. Involvement of neutrophils and interleukin-18 in nociception in a mouse model of muscle pain. Mol Pain. 2018;14:1744806918757286 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Klein J, Moses K, Zelinskyy G, Sody S, Buer J, Lang S, et al. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth. Nat Commun. 2017;8:14600 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2o
Zhu Y, Lyapichev K, Lee D, Motti D, Ferraro N, Zhang Y, et al. Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury. J Neurosci. 2017;37:2362-2376 pubmed publisher
  • flow cytometry; mouse; fig 4
Baillet A, Rehaume L, Benham H, O Meara C, Armitage C, Ruscher R, et al. High Chlamydia Burden Promotes Tumor Necrosis Factor-Dependent Reactive Arthritis in SKG Mice. Arthritis Rheumatol. 2015;67:1535-47 pubmed publisher
Zhang T, Wang Y, Li Q, Lin L, Xu C, Xue Y, et al. Mesenchymal stromal cells equipped by IFNα empower T cells with potent anti-tumor immunity. Oncogene. 2022;41:1866-1881 pubmed publisher
Hohsfield L, Najafi A, Ghorbanian Y, Soni N, Crapser J, Figueroa Velez D, et al. Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave. elife. 2021;10: pubmed publisher
Hildebrand K, Singla A, McNeil R, Marritt K, Hildebrand K, Zemp F, et al. The KrasG12D;Trp53fl/fl murine model of undifferentiated pleomorphic sarcoma is macrophage dense, lymphocyte poor, and resistant to immune checkpoint blockade. PLoS ONE. 2021;16:e0253864 pubmed publisher
Milich L, Choi J, Ryan C, Cerqueira S, Benavides S, Yahn S, et al. Single-cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord. J Exp Med. 2021;218: pubmed publisher
Verma M, Michalec L, Sripada A, McKay J, Sirohi K, Verma D, et al. The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma. J Exp Med. 2021;218: pubmed publisher
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
Dai X, Bu X, Gao Y, Guo J, Hu J, Jiang C, et al. Energy status dictates PD-L1 protein abundance and anti-tumor immunity to enable checkpoint blockade. Mol Cell. 2021;81:2317-2331.e6 pubmed publisher
Dang M, Gonzalez M, Gaonkar K, Rathi K, Young P, Arif S, et al. Macrophages in SHH subgroup medulloblastoma display dynamic heterogeneity that varies with treatment modality. Cell Rep. 2021;34:108917 pubmed publisher
Zhang Y, Qin Y, Chen Z. Neuromedin U Suppresses Collagen-Induced Arthritis through ILC2-Th2 Activation. J Immunol Res. 2021;2021:5599439 pubmed publisher
Gern B, Adams K, Plumlee C, Stoltzfus C, Shehata L, Moguche A, et al. TGFβ restricts expansion, survival, and function of T cells within the tuberculous granuloma. Cell Host Microbe. 2021;29:594-606.e6 pubmed publisher
Desai P, Janova H, White J, Reynoso G, Hickman H, Baldridge M, et al. Enteric helminth coinfection enhances host susceptibility to neurotropic flaviviruses via a tuft cell-IL-4 receptor signaling axis. Cell. 2021;184:1214-1231.e16 pubmed publisher
Rodriguez Garcia A, Lynn R, Poussin M, Eiva M, Shaw L, O Connor R, et al. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy. Nat Commun. 2021;12:877 pubmed publisher
Karki R, Sharma B, Tuladhar S, Williams E, Zalduondo L, Samir P, et al. Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes. Cell. 2021;184:149-168.e17 pubmed publisher
Plumlee C, Duffy F, Gern B, Delahaye J, Cohen S, Stoltzfus C, et al. Ultra-low Dose Aerosol Infection of Mice with Mycobacterium tuberculosis More Closely Models Human Tuberculosis. Cell Host Microbe. 2021;29:68-82.e5 pubmed publisher
Liang W, Peng X, Li Q, Wang P, Lv P, Song Q, et al. FAM3D is essential for colon homeostasis and host defense against inflammation associated carcinogenesis. Nat Commun. 2020;11:5912 pubmed publisher
Trivedi S, Labuz D, Anderson C, Araújo C, Blair A, Middleton E, et al. Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis. elife. 2020;9: pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Kobia F, Preusse K, Dai Q, Weaver N, Hass M, Chaturvedi P, et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020;18:e3000850 pubmed publisher
Xu G, van Bruggen R, Gualtieri C, Moradin N, Fois A, Vallerand D, et al. Bisphosphoglycerate Mutase Deficiency Protects against Cerebral Malaria and Severe Malaria-Induced Anemia. Cell Rep. 2020;32:108170 pubmed publisher
Doty D, Schueler J, Mott V, Bryan C, Moore N, Ho J, et al. Modeling Immune Checkpoint Inhibitor Efficacy in Syngeneic Mouse Tumors in an Ex Vivo Immuno-Oncology Dynamic Environment. Int J Mol Sci. 2020;21: pubmed publisher
Nakayama Y, Fujiu K, Yuki R, Oishi Y, Morioka M, Isagawa T, et al. A long noncoding RNA regulates inflammation resolution by mouse macrophages through fatty acid oxidation activation. Proc Natl Acad Sci U S A. 2020;117:14365-14375 pubmed publisher
Riquelme S, Liimatta K, Wong Fok Lung T, Fields B, Ahn D, Chen D, et al. Pseudomonas aeruginosa Utilizes Host-Derived Itaconate to Redirect Its Metabolism to Promote Biofilm Formation. Cell Metab. 2020;31:1091-1106.e6 pubmed publisher
Yeung F, Chen Y, Lin J, Leung J, McCauley C, Devlin J, et al. Altered Immunity of Laboratory Mice in the Natural Environment Is Associated with Fungal Colonization. Cell Host Microbe. 2020;27:809-822.e6 pubmed publisher
Ireland L, Luckett T, Schmid M, Mielgo A. Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis. Front Immunol. 2020;11:297 pubmed publisher
Muri J, Thut H, Feng Q, Kopf M. Thioredoxin-1 distinctly promotes NF-κB target DNA binding and NLRP3 inflammasome activation independently of Txnip. elife. 2020;9: pubmed publisher
Blagih J, Zani F, Chakravarty P, Hennequart M, Pilley S, Hobor S, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30:481-496.e6 pubmed publisher
Bhattacherjee A, Rodrigues E, Jung J, Luzentales Simpson M, Enterina J, Galleguillos D, et al. Repression of phagocytosis by human CD33 is not conserved with mouse CD33. Commun Biol. 2019;2:450 pubmed publisher
Leech J, Dhariwala M, Lowe M, Chu K, Merana G, Cornuot C, et al. Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria. Cell Host Microbe. 2019;26:795-809.e5 pubmed publisher
Sun X, Seidman J, Zhao P, Troutman T, Spann N, Que X, et al. Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic Steatohepatitis. Cell Metab. 2020;31:189-206.e8 pubmed publisher
Körner A, Zhou E, Müller C, Mohammed Y, Herceg S, Bracher F, et al. Inhibition of Δ24-dehydrocholesterol reductase activates pro-resolving lipid mediator biosynthesis and inflammation resolution. Proc Natl Acad Sci U S A. 2019;116:20623-20634 pubmed publisher
Acker K, Wong Fok Lung T, West E, Craft J, Narechania A, Smith H, et al. Strains of Staphylococcus aureus that Colonize and Infect Skin Harbor Mutations in Metabolic Genes. iScience. 2019;19:281-290 pubmed publisher
Merz S, Korste S, Bornemann L, Michel L, Stock P, Squire A, et al. Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response. Nat Commun. 2019;10:2312 pubmed publisher
Misumi I, Cook K, Mitchell J, Lund M, Vick S, Lee R, et al. Identification of a Locus in Mice that Regulates the Collateral Damage and Lethality of Virus Infection. Cell Rep. 2019;27:1387-1396.e5 pubmed publisher
Körner A, Schlegel M, Kaussen T, Gudernatsch V, Hansmann G, Schumacher T, et al. Sympathetic nervous system controls resolution of inflammation via regulation of repulsive guidance molecule A. Nat Commun. 2019;10:633 pubmed publisher
Salazar V, Jagger B, Mongkolsapaya J, Burgomaster K, Dejnirattisai W, Winkler E, et al. Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice. Cell Rep. 2019;26:1585-1597.e4 pubmed publisher
Funk K, Klein R. CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis. J Neuroinflammation. 2019;16:22 pubmed publisher
Chan W, Entwisle C, Ercoli G, Ramos Sevillano E, McIlgorm A, Cecchini P, et al. A Novel, Multiple-Antigen Pneumococcal Vaccine Protects against Lethal Streptococcus pneumoniae Challenge. Infect Immun. 2019;87: pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Schlegel M, Körner A, Kaussen T, Knausberg U, Gerber C, Hansmann G, et al. Inhibition of neogenin fosters resolution of inflammation and tissue regeneration. J Clin Invest. 2018;128:4711-4726 pubmed publisher
Roediger B, Lee Q, Tikoo S, Cobbin J, Henderson J, Jormakka M, et al. An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis. Cell. 2018;175:530-543.e24 pubmed publisher
Cohen S, Gern B, Delahaye J, Adams K, Plumlee C, Winkler J, et al. Alveolar Macrophages Provide an Early Mycobacterium tuberculosis Niche and Initiate Dissemination. Cell Host Microbe. 2018;24:439-446.e4 pubmed publisher
Cho S, Vardi A, Platt N, Futerman A. Absence of infiltrating peripheral myeloid cells in the brains of mouse models of lysosomal storage disorders. J Neurochem. 2019;148:625-638 pubmed publisher
Bennett F, Bennett M, Yaqoob F, Mulinyawe S, Grant G, Hayden Gephart M, et al. A Combination of Ontogeny and CNS Environment Establishes Microglial Identity. Neuron. 2018;98:1170-1183.e8 pubmed publisher
Privratsky J, Zhang J, Lu X, Rudemiller N, Wei Q, Yu Y, et al. Interleukin 1 receptor (IL-1R1) activation exacerbates toxin-induced acute kidney injury. Am J Physiol Renal Physiol. 2018;315:F682-F691 pubmed publisher
Niknam S, Barsoumian H, Schoenhals J, Jackson H, Yanamandra N, Caetano M, et al. Radiation Followed by OX40 Stimulation Drives Local and Abscopal Antitumor Effects in an Anti-PD1-Resistant Lung Tumor Model. Clin Cancer Res. 2018;24:5735-5743 pubmed publisher
Haertel E, Joshi N, Hiebert P, Kopf M, Werner S. Regulatory T cells are required for normal and activin-promoted wound repair in mice. Eur J Immunol. 2018;48:1001-1013 pubmed publisher
Syed I, Lee J, Moraes Vieira P, Donaldson C, Sontheimer A, Aryal P, et al. Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab. 2018;27:419-427.e4 pubmed publisher
Maschmeyer P, Petkau G, Siracusa F, Zimmermann J, Zügel F, Kühl A, et al. Selective targeting of pro-inflammatory Th1 cells by microRNA-148a-specific antagomirs in vivo. J Autoimmun. 2018;89:41-52 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Mattila J, Beaino W, Maiello P, Coleman M, White A, Scanga C, et al. Positron Emission Tomography Imaging of Macaques with Tuberculosis Identifies Temporal Changes in Granuloma Glucose Metabolism and Integrin ?4?1-Expressing Immune Cells. J Immunol. 2017;199:806-815 pubmed publisher
Smith A, Smith A. A Critical, Nonlinear Threshold Dictates Bacterial Invasion and Initial Kinetics During Influenza. Sci Rep. 2016;6:38703 pubmed publisher
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed publisher
Zhang B, Kwon O, Henry G, Malewska A, Wei X, Zhang L, et al. Non-Cell-Autonomous Regulation of Prostate Epithelial Homeostasis by Androgen Receptor. Mol Cell. 2016;63:976-89 pubmed publisher
Maisel D, Birzele F, Voss E, Nopora A, Bader S, Friess T, et al. Targeting Tumor Cells with Anti-CD44 Antibody Triggers Macrophage-Mediated Immune Modulatory Effects in a Cancer Xenograft Model. PLoS ONE. 2016;11:e0159716 pubmed publisher
Chen Z, Andreev D, Oeser K, Krljanac B, Hueber A, Kleyer A, et al. Th2 and eosinophil responses suppress inflammatory arthritis. Nat Commun. 2016;7:11596 pubmed publisher
Shepardson K, Larson K, Morton R, Prigge J, Schmidt E, Huber V, et al. Differential Type I Interferon Signaling Is a Master Regulator of Susceptibility to Postinfluenza Bacterial Superinfection. MBio. 2016;7: pubmed publisher
Kwon O, Zhang B, Zhang L, Xin L. High fat diet promotes prostatic basal-to-luminal differentiation and accelerates initiation of prostate epithelial hyperplasia originated from basal cells. Stem Cell Res. 2016;16:682-91 pubmed publisher
Gurung P, Burton A, Kanneganti T. NLRP3 inflammasome plays a redundant role with caspase 8 to promote IL-1?-mediated osteomyelitis. Proc Natl Acad Sci U S A. 2016;113:4452-7 pubmed publisher
Su N, Yue Y, Xiong S. Monocytic myeloid-derived suppressor cells from females, but not males, alleviate CVB3-induced myocarditis by increasing regulatory and CD4(+)IL-10(+) T cells. Sci Rep. 2016;6:22658 pubmed publisher
Moses K, Klein J, Männ L, Klingberg A, Gunzer M, Brandau S. Survival of residual neutrophils and accelerated myelopoiesis limit the efficacy of antibody-mediated depletion of Ly-6G+ cells in tumor-bearing mice. J Leukoc Biol. 2016;99:811-23 pubmed publisher
Kim I, Mlsna L, Yoon S, Le B, Yu S, Xu D, et al. A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers. Brain Behav. 2015;5:e00403 pubmed publisher
Jablonski K, Amici S, Webb L, Ruiz Rosado J, Popovich P, Partida Sanchez S, et al. Novel Markers to Delineate Murine M1 and M2 Macrophages. PLoS ONE. 2015;10:e0145342 pubmed publisher
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Garcia Bonilla L, Racchumi G, Murphy M, Anrather J, Iadecola C. Endothelial CD36 Contributes to Postischemic Brain Injury by Promoting Neutrophil Activation via CSF3. J Neurosci. 2015;35:14783-93 pubmed publisher
Ballet R, Emre Y, Jemelin S, Charmoy M, Tacchini Cottier F, Imhof B. Blocking junctional adhesion molecule C enhances dendritic cell migration and boosts the immune responses against Leishmania major. PLoS Pathog. 2014;10:e1004550 pubmed publisher
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Kwon O, Zhang L, Ittmann M, Xin L. Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin. Proc Natl Acad Sci U S A. 2014;111:E592-600 pubmed publisher
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Sumagin R, Prizant H, Lomakina E, Waugh R, Sarelius I. LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ. J Immunol. 2010;185:7057-66 pubmed publisher
Tadagavadi R, REEVES W. Endogenous IL-10 attenuates cisplatin nephrotoxicity: role of dendritic cells. J Immunol. 2010;185:4904-11 pubmed publisher
Daley J, Thomay A, Connolly M, Reichner J, Albina J. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol. 2008;83:64-70 pubmed
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC
Cat # :
127615
Clone :
1A8
Item :
PerCP/Cyanine5.5 anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
25 μg
Price (USD) :
112 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA