product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse Ly-6G
catalog :
127612
quantity :
100 μg
price :
248 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
1A8
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 62
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig e7b
Fischer A, Wannemacher J, Christ S, Koopmans T, Kadri S, Zhao J, et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol. 2022;23:518-531 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Chen K, Hu Q, Xie Z, Yang G. Monocyte NLRP3-IL-1β Hyperactivation Mediates Neuronal and Synaptic Dysfunction in Perioperative Neurocognitive Disorder. Adv Sci (Weinh). 2022;9:e2104106 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, s2a, s2b, s2k
Khaw Y, Majid D, Oh S, Kang E, Inoue M. Early-life-trauma triggers interferon-β resistance and neurodegeneration in a multiple sclerosis model via downregulated β1-adrenergic signaling. Nat Commun. 2021;12:105 pubmed publisher
  • flow cytometry; mouse; loading ...
Myers D, Abram C, Wildes D, Belwafa A, Welsh A, Schulze C, et al. Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity. Front Immunol. 2020;11:576310 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2f, s2e
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s2d
Mylonas K, Turner N, Bageghni S, Kenyon C, White C, McGregor K, et al. 11β-HSD1 suppresses cardiac fibroblast CXCL2, CXCL5 and neutrophil recruitment to the heart post MI. J Endocrinol. 2017;233:315-327 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Herz J, Johnson K, McGavern D. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. J Exp Med. 2015;212:1153-69 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 3
  • flow cytometry; mouse; fig 4
Her Z, Teng T, Tan J, Teo T, Kam Y, Lum F, et al. Loss of TLR3 aggravates CHIKV replication and pathology due to an altered virus-specific neutralizing antibody response. EMBO Mol Med. 2015;7:24-41 pubmed publisher
  • flow cytometry; mouse; fig 4
Lieber S, Scheer F, Finkernagel F, Meissner W, Giehl G, Brendel C, et al. The inverse agonist DG172 triggers a PPARβ/δ-independent myeloid lineage shift and promotes GM-CSF/IL-4-induced dendritic cell differentiation. Mol Pharmacol. 2015;87:162-73 pubmed publisher
Li F, Han D, Wang B, Zhang W, Zhao Y, Xu J, et al. Topical Treatment of Colquhounia Root Relieves Skin Inflammation and Itch in Imiquimod-Induced Psoriasiform Dermatitis in Mice. Mediators Inflamm. 2022;2022:5782922 pubmed publisher
Anderson A, Wu I, Parrillo A, Wolf M, Maestas D, Graham I, et al. An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial. NPJ Regen Med. 2022;7:6 pubmed publisher
Honda M, Kadohisa M, Yoshii D, Komohara Y, Hibi T. Directly recruited GATA6 + peritoneal cavity macrophages contribute to the repair of intestinal serosal injury. Nat Commun. 2021;12:7294 pubmed publisher
de Gaetano M, Tighe C, Gahan K, Zanetti A, Chen J, Newson J, et al. Asymmetric Synthesis and Biological Screening of Quinoxaline-Containing Synthetic Lipoxin A4 Mimetics (QNX-sLXms). J Med Chem. 2021;64:9193-9216 pubmed publisher
Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen Woods C, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021;54:1494-1510.e7 pubmed publisher
Kumari P, Russo A, Wright S, Muthupalani S, Rathinam V. Hierarchical cell-type-specific functions of caspase-11 in LPS shock and antibacterial host defense. Cell Rep. 2021;35:109012 pubmed publisher
Yilmaz B, Mooser C, Keller I, Li H, Zimmermann J, Bosshard L, et al. Long-term evolution and short-term adaptation of microbiota strains and sub-strains in mice. Cell Host Microbe. 2021;29:650-663.e9 pubmed publisher
Wessel A, Kose N, Bombardi R, Roy V, Chantima W, Mongkolsapaya J, et al. Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy. Nat Commun. 2020;11:5278 pubmed publisher
Shemer A, Scheyltjens I, Frumer G, Kim J, Grozovski J, Ayanaw S, et al. Interleukin-10 Prevents Pathological Microglia Hyperactivation following Peripheral Endotoxin Challenge. Immunity. 2020;53:1033-1049.e7 pubmed publisher
Sharma A, Seow J, Dutertre C, Pai R, Blériot C, Mishra A, et al. Onco-fetal Reprogramming of Endothelial Cells Drives Immunosuppressive Macrophages in Hepatocellular Carcinoma. Cell. 2020;183:377-394.e21 pubmed publisher
Neupane A, Willson M, Chojnacki A, Vargas E Silva Castanheira F, Morehouse C, Carestia A, et al. Patrolling Alveolar Macrophages Conceal Bacteria from the Immune System to Maintain Homeostasis. Cell. 2020;183:110-125.e11 pubmed publisher
Hooftman A, Angiari S, Hester S, Corcoran S, Runtsch M, Ling C, et al. The Immunomodulatory Metabolite Itaconate Modifies NLRP3 and Inhibits Inflammasome Activation. Cell Metab. 2020;32:468-478.e7 pubmed publisher
Fukushima K, Satoh T, Sugihara F, Sato Y, Okamoto T, Mitsui Y, et al. Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis. Immunity. 2020;52:542-556.e13 pubmed publisher
Yamada K, Heim C, Xi X, Attri K, Wang D, Zhang W, et al. Monocyte metabolic reprogramming promotes pro-inflammatory activity and Staphylococcus aureus biofilm clearance. PLoS Pathog. 2020;16:e1008354 pubmed publisher
Goldstein J, Valido A, Lewandowski J, Walker R, Mills M, Messemer K, et al. Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Sci Rep. 2019;9:18613 pubmed publisher
Davis F, Schaller M, denDekker A, Joshi A, Kimball A, Evanoff H, et al. Sepsis Induces Prolonged Epigenetic Modifications in Bone Marrow and Peripheral Macrophages Impairing Inflammation and Wound Healing. Arterioscler Thromb Vasc Biol. 2019;39:2353-2366 pubmed publisher
Deniset J, Belke D, Lee W, Jorch S, Deppermann C, Hassanabad A, et al. Gata6+ Pericardial Cavity Macrophages Relocate to the Injured Heart and Prevent Cardiac Fibrosis. Immunity. 2019;51:131-140.e5 pubmed publisher
Hou X, Chen G, Bracamonte Baran W, Choi H, Diny N, Sung J, et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep. 2019;28:172-189.e7 pubmed publisher
Abe H, Takeda N, Isagawa T, Semba H, Nishimura S, Morioka M, et al. Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M. Nat Commun. 2019;10:2824 pubmed publisher
Goldstein J, Tabebordbar M, Zhu K, Wang L, Messemer K, Peacker B, et al. In Situ Modification of Tissue Stem and Progenitor Cell Genomes. Cell Rep. 2019;27:1254-1264.e7 pubmed publisher
Jung J, Yoo J, Choe Y, Park S, Lee H, Lee H, et al. Cleaved Cochlin Sequesters Pseudomonas aeruginosa and Activates Innate Immunity in the Inner Ear. Cell Host Microbe. 2019;25:513-525.e6 pubmed publisher
Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
Teo T, Lum F, Ghaffar K, Chan Y, Amrun S, Tan J, et al. Plasmodium co-infection protects against chikungunya virus-induced pathologies. Nat Commun. 2018;9:3905 pubmed publisher
McCreedy D, Lee S, Sontag C, Weinstein P, Olivas A, Martinez A, et al. Early Targeting of L-Selectin on Leukocytes Promotes Recovery after Spinal Cord Injury, Implicating Novel Mechanisms of Pathogenesis. Eneuro. 2018;5: pubmed publisher
Shavit Stein E, Ben Shimon M, Artan Furman A, Golderman V, Chapman J, Maggio N. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment. Neural Plast. 2018;2018:7692182 pubmed publisher
Linde N, Casanova Acebes M, Sosa M, Mortha A, Rahman A, Farias E, et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat Commun. 2018;9:21 pubmed publisher
Varol D, Mildner A, Blank T, Shemer A, Barashi N, Yona S, et al. Dicer Deficiency Differentially Impacts Microglia of the Developing and Adult Brain. Immunity. 2017;46:1030-1044.e8 pubmed publisher
Mishra B, Lovewell R, Olive A, Zhang G, Wang W, Eugenin E, et al. Nitric oxide prevents a pathogen-permissive granulocytic inflammation during tuberculosis. Nat Microbiol. 2017;2:17072 pubmed publisher
Gittens B, Bodkin J, Nourshargh S, Perretti M, Cooper D. Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation. J Immunol. 2017;198:4458-4469 pubmed publisher
Campa C, Germena G, Ciraolo E, Copperi F, Sapienza A, Franco I, et al. Rac signal adaptation controls neutrophil mobilization from the bone marrow. Sci Signal. 2016;9:ra124 pubmed publisher
Sadtler K, Allen B, Estrellas K, Housseau F, Pardoll D, Elisseeff J. The Scaffold Immune Microenvironment: Biomaterial-Mediated Immune Polarization in Traumatic and Nontraumatic Applications<sup/>. Tissue Eng Part A. 2017;23:1044-1053 pubmed publisher
Chew W, Tabebordbar M, Cheng J, Mali P, Wu E, Ng A, et al. A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016;13:868-74 pubmed publisher
Wang B, Wang X, Wen Y, Fu J, Wang H, Ma Z, et al. Suppression of established hepatocarcinoma in adjuvant only immunotherapy: alum triggers anti-tumor CD8+ T cell response. Sci Rep. 2015;5:17695 pubmed publisher
Huang W, Morales J, Gazivoda V, August A. Nonreceptor tyrosine kinases ITK and BTK negatively regulate mast cell proinflammatory responses to lipopolysaccharide. J Allergy Clin Immunol. 2016;137:1197-1205 pubmed publisher
Cardona S, Mendiola A, Yang Y, Adkins S, Torres V, Cardona A. Disruption of Fractalkine Signaling Leads to Microglial Activation and Neuronal Damage in the Diabetic Retina. ASN Neuro. 2015;7: pubmed publisher
Calderon B, Carrero J, Ferris S, Sojka D, Moore L, Epelman S, et al. The pancreas anatomy conditions the origin and properties of resident macrophages. J Exp Med. 2015;212:1497-512 pubmed publisher
Bodogai M, Moritoh K, Lee Chang C, Hollander C, Sherman Baust C, Wersto R, et al. Immunosuppressive and Prometastatic Functions of Myeloid-Derived Suppressive Cells Rely upon Education from Tumor-Associated B Cells. Cancer Res. 2015;75:3456-65 pubmed publisher
Teo T, Her Z, Tan J, Lum F, Lee W, Chan Y, et al. Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology. J Virol. 2015;89:7955-69 pubmed publisher
Zhang L, Liu S, Wright T, Shen Z, Li H, Zhu W, et al. C-reactive protein directly suppresses Th1 cell differentiation and alleviates experimental autoimmune encephalomyelitis. J Immunol. 2015;194:5243-52 pubmed publisher
Mylonas K, Jenkins S, Castellan R, Rückerl D, McGregor K, Phythian Adams A, et al. The adult murine heart has a sparse, phagocytically active macrophage population that expands through monocyte recruitment and adopts an 'M2' phenotype in response to Th2 immunologic challenge. Immunobiology. 2015;220:924-33 pubmed publisher
Kim K, Skora A, Li Z, Liu Q, Tam A, Blosser R, et al. Eradication of metastatic mouse cancers resistant to immune checkpoint blockade by suppression of myeloid-derived cells. Proc Natl Acad Sci U S A. 2014;111:11774-9 pubmed publisher
McCartney Francis N, Jin W, Belkaid Y, McGrady G, Wahl S. Aberrant host defense against Leishmania major in the absence of SLPI. J Leukoc Biol. 2014;96:917-29 pubmed publisher
Dorward D, Lucas C, Alessandri A, Marwick J, Rossi F, Dransfield I, et al. Technical advance: autofluorescence-based sorting: rapid and nonperturbing isolation of ultrapure neutrophils to determine cytokine production. J Leukoc Biol. 2013;94:193-202 pubmed publisher
Hickey A, Lin J, Kummer L, Szaba F, Duso D, Tighe M, et al. Intranasal prophylaxis with CpG oligodeoxynucleotide can protect against Yersinia pestis infection. Infect Immun. 2013;81:2123-32 pubmed publisher
Avila M, Martínez Juárez A, Ibarra Sanchez A, Gonzalez Espinosa C. Lyn kinase controls TLR4-dependent IKK and MAPK activation modulating the activity of TRAF-6/TAK-1 protein complex in mast cells. Innate Immun. 2012;18:648-60 pubmed publisher
Yan J, Meng X, Wancket L, Lintner K, Nelin L, Chen B, et al. Glutathione reductase facilitates host defense by sustaining phagocytic oxidative burst and promoting the development of neutrophil extracellular traps. J Immunol. 2012;188:2316-27 pubmed publisher
Ng L, Qin J, Roediger B, Wang Y, Jain R, Cavanagh L, et al. Visualizing the neutrophil response to sterile tissue injury in mouse dermis reveals a three-phase cascade of events. J Invest Dermatol. 2011;131:2058-68 pubmed publisher
Gibbons M, Mackinnon A, Ramachandran P, Dhaliwal K, Duffin R, Phythian Adams A, et al. Ly6Chi monocytes direct alternatively activated profibrotic macrophage regulation of lung fibrosis. Am J Respir Crit Care Med. 2011;184:569-81 pubmed publisher
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Guiducci C, Tripodo C, Gong M, Sangaletti S, Colombo M, Coffman R, et al. Autoimmune skin inflammation is dependent on plasmacytoid dendritic cell activation by nucleic acids via TLR7 and TLR9. J Exp Med. 2010;207:2931-42 pubmed publisher
Sumagin R, Prizant H, Lomakina E, Waugh R, Sarelius I. LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ. J Immunol. 2010;185:7057-66 pubmed publisher
Tadagavadi R, REEVES W. Endogenous IL-10 attenuates cisplatin nephrotoxicity: role of dendritic cells. J Immunol. 2010;185:4904-11 pubmed publisher
Daley J, Thomay A, Connolly M, Reichner J, Albina J. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol. 2008;83:64-70 pubmed
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC
Cat # :
127612
Clone :
1A8
Item :
Pacific Blue™ anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
100 μg
Price (USD) :
248 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA