product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse Ly-6G
catalog :
127603
quantity :
50 μg
price :
77 USD
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
1A8
reactivity :
mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
citations: 54
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...; fig 1a, 1d
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; fig 8A
Vila Leahey A, Oldford S, Marignani P, Wang J, Haidl I, Marshall J. Ranitidine modifies myeloid cell populations and inhibits breast tumor development and spread in mice. Oncoimmunology. 2016;5:e1151591 pubmed publisher
  • immunohistochemistry; mouse; 1:150
Chen W, Cao Z, Sugaya S, Lopez M, Sendra V, Laver N, et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun. 2016;7:11302 pubmed publisher
  • flow cytometry; mouse; tbl 1
  • immunocytochemistry; mouse; tbl 1
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-364 pubmed publisher
Schäfer A, Eichhorst A, Hentze C, Kraemer A, Amend A, Sprenger D, et al. Low Dietary Fiber Intake Links Development of Obesity and Lupus Pathogenesis. Front Immunol. 2021;12:696810 pubmed publisher
Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen Woods C, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021;54:1494-1510.e7 pubmed publisher
Huang X, Ferris S, Kim S, Choudhary M, Belk J, Fan C, et al. Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis. Immunity. 2021;54:1417-1432.e7 pubmed publisher
de Mingo Pulido Á, Hänggi K, Celias D, Gardner A, Li J, Batista Bittencourt B, et al. The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake. Immunity. 2021;54:1154-1167.e7 pubmed publisher
Muthukrishnan G, Wallimann A, Rangel Moreno J, Bentley K, Hildebrand M, Mys K, et al. Humanized Mice Exhibit Exacerbated Abscess Formation and Osteolysis During the Establishment of Implant-Associated Staphylococcus aureus Osteomyelitis. Front Immunol. 2021;12:651515 pubmed publisher
Levine L, Hiam Galvez K, Marquez D, Tenvooren I, Madden M, Contreras D, et al. Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8+ T cells during the primary immune response. Immunity. 2021;54:829-844.e5 pubmed publisher
Moorlag S, Rodriguez Rosales Y, Gillard J, Fanucchi S, Theunissen K, Novakovic B, et al. BCG Vaccination Induces Long-Term Functional Reprogramming of Human Neutrophils. Cell Rep. 2020;33:108387 pubmed publisher
Kalafati L, Kourtzelis I, Schulte Schrepping J, Li X, Hatzioannou A, Grinenko T, et al. Innate Immune Training of Granulopoiesis Promotes Anti-tumor Activity. Cell. 2020;183:771-785.e12 pubmed publisher
Kim S, Bagadia P, Anderson D, Liu T, Huang X, Theisen D, et al. High Amount of Transcription Factor IRF8 Engages AP1-IRF Composite Elements in Enhancers to Direct Type 1 Conventional Dendritic Cell Identity. Immunity. 2020;53:759-774.e9 pubmed publisher
Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart B, Brouilly N, et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages. Immunity. 2020;53:127-142.e7 pubmed publisher
Soni C, Perez O, Voss W, Pucella J, Serpas L, Mehl J, et al. Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA. Immunity. 2020;52:1022-1038.e7 pubmed publisher
Bosteels C, Neyt K, Vanheerswynghels M, Van Helden M, Sichien D, Debeuf N, et al. Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection. Immunity. 2020;52:1039-1056.e9 pubmed publisher
Chauveau A, Pirgova G, Cheng H, De Martin A, Zhou F, Wideman S, et al. Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones. Immunity. 2020;52:794-807.e7 pubmed publisher
Han P, Hanlon D, Arshad N, Lee J, Tatsuno K, Robinson E, et al. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Sci Adv. 2020;6:eaaz1580 pubmed publisher
Khaw Y, Cunningham C, Tierney A, Sivaguru M, Inoue M. Neutrophil-selective deletion of Cxcr2 protects against CNS neurodegeneration in a mouse model of multiple sclerosis. J Neuroinflammation. 2020;17:49 pubmed publisher
Kimball A, Obi A, Luke C, Dowling A, Cai Q, Adili R, et al. Ly6CLo Monocyte/Macrophages are Essential for Thrombus Resolution in a Murine Model of Venous Thrombosis. Thromb Haemost. 2020;120:289-299 pubmed publisher
Cai W, Wang J, Hu M, Chen X, Lu Z, Bellanti J, et al. All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling. J Neuroinflammation. 2019;16:175 pubmed publisher
Bagadia P, Huang X, Liu T, Durai V, Grajales Reyes G, Nitschké M, et al. An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 development. Nat Immunol. 2019;20:1174-1185 pubmed publisher
Kimball A, Davis F, denDekker A, Joshi A, Schaller M, Bermick J, et al. The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair. Immunity. 2019;51:258-271.e5 pubmed publisher
Evans R, Flores Borja F, Nassiri S, Miranda E, Lawler K, Grigoriadis A, et al. Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer. Cell Rep. 2019;27:1967-1978.e4 pubmed publisher
Haffner Luntzer M, Foertsch S, Fischer V, Prystaz K, Tschaffon M, Mödinger Y, et al. Chronic psychosocial stress compromises the immune response and endochondral ossification during bone fracture healing via β-AR signaling. Proc Natl Acad Sci U S A. 2019;116:8615-8622 pubmed publisher
Lerman I, Ma X, Seger C, Maolake A, Garcia Hernandez M, Rangel Moreno J, et al. Epigenetic Suppression of SERPINB1 Promotes Inflammation-Mediated Prostate Cancer Progression. Mol Cancer Res. 2019;17:845-859 pubmed publisher
Vaibhav K, Braun M, Khan M, Fatima S, Saad N, Shankar A, et al. Remote ischemic post-conditioning promotes hematoma resolution via AMPK-dependent immune regulation. J Exp Med. 2018;215:2636-2654 pubmed publisher
Best S, De Souza D, Kersbergen A, Policheni A, Dayalan S, Tull D, et al. Synergy between the KEAP1/NRF2 and PI3K Pathways Drives Non-Small-Cell Lung Cancer with an Altered Immune Microenvironment. Cell Metab. 2018;27:935-943.e4 pubmed publisher
Kimball A, Schaller M, Joshi A, Davis F, denDekker A, Boniakowski A, et al. Ly6CHi Blood Monocyte/Macrophage Drive Chronic Inflammation and Impair Wound Healing in Diabetes Mellitus. Arterioscler Thromb Vasc Biol. 2018;38:1102-1114 pubmed publisher
Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
de Mingo Pulido Á, Gardner A, Hiebler S, Soliman H, Rugo H, Krummel M, et al. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell. 2018;33:60-74.e6 pubmed publisher
Duarte D, Hawkins E, Akinduro O, Ang H, De Filippo K, Kong I, et al. Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML. Cell Stem Cell. 2018;22:64-77.e6 pubmed publisher
Xu L, Huang Q, Wang H, Hao Y, Bai Q, Hu J, et al. The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells. Immunity. 2017;47:538-551.e5 pubmed publisher
Haffner Luntzer M, Fischer V, Prystaz K, Liedert A, Ignatius A. The inflammatory phase of fracture healing is influenced by oestrogen status in mice. Eur J Med Res. 2017;22:23 pubmed publisher
Domínguez Andrés J, Feo Lucas L, Minguito de la Escalera M, Gonzalez L, López Bravo M, Ardavin C. Inflammatory Ly6Chigh Monocytes Protect against Candidiasis through IL-15-Driven NK Cell/Neutrophil Activation. Immunity. 2017;46:1059-1072.e4 pubmed publisher
Sahakian E, Chen J, Powers J, Chen X, Maharaj K, Deng S, et al. Essential role for histone deacetylase 11 (HDAC11) in neutrophil biology. J Leukoc Biol. 2017;102:475-486 pubmed publisher
Hulsmans M, Clauss S, Xiao L, Aguirre A, King K, Hanley A, et al. Macrophages Facilitate Electrical Conduction in the Heart. Cell. 2017;169:510-522.e20 pubmed publisher
Kotfis K, Zegan Barańska M, Szydłowski Ł, Zukowski M, Ely E. Methods of pain assessment in adult intensive care unit patients - Polish version of the CPOT (Critical Care Pain Observation Tool) and BPS (Behavioral Pain Scale). Anaesthesiol Intensive Ther. 2017;49:66-72 pubmed publisher
Popova T, Teunis A, Espina V, Liotta L, Popov S. Chemokine-Releasing Microparticles Improve Bacterial Clearance and Survival of Anthrax Spore-Challenged Mice. PLoS ONE. 2016;11:e0163163 pubmed publisher
De Cock J, Shibue T, Dongre A, Keckesova Z, Reinhardt F, Weinberg R. Inflammation Triggers Zeb1-Dependent Escape from Tumor Latency. Cancer Res. 2016;76:6778-6784 pubmed
Tang Y, Fickert P, Trauner M, Marcus N, Blomenkamp K, Teckman J. Autophagy induced by exogenous bile acids is therapeutic in a model of ?-1-AT deficiency liver disease. Am J Physiol Gastrointest Liver Physiol. 2016;311:G156-65 pubmed publisher
Periasamy S, Avram D, McCabe A, MacNamara K, Sellati T, Harton J. An Immature Myeloid/Myeloid-Suppressor Cell Response Associated with Necrotizing Inflammation Mediates Lethal Pulmonary Tularemia. PLoS Pathog. 2016;12:e1005517 pubmed publisher
Steiger S, Kuhn S, Ronchese F, Harper J. Monosodium Urate Crystals Induce Upregulation of NK1.1-Dependent Killing by Macrophages and Support Tumor-Resident NK1.1+ Monocyte/Macrophage Populations in Antitumor Therapy. J Immunol. 2015;195:5495-502 pubmed publisher
Blazek K, Eames H, WEISS M, Byrne A, Perocheau D, Pease J, et al. IFN-λ resolves inflammation via suppression of neutrophil infiltration and IL-1β production. J Exp Med. 2015;212:845-53 pubmed publisher
Her Z, Teng T, Tan J, Teo T, Kam Y, Lum F, et al. Loss of TLR3 aggravates CHIKV replication and pathology due to an altered virus-specific neutralizing antibody response. EMBO Mol Med. 2015;7:24-41 pubmed publisher
McCartney Francis N, Jin W, Belkaid Y, McGrady G, Wahl S. Aberrant host defense against Leishmania major in the absence of SLPI. J Leukoc Biol. 2014;96:917-29 pubmed publisher
Yang L, Feng D, Bi X, Stone R, Barnes B. Monocytes from Irf5-/- mice have an intrinsic defect in their response to pristane-induced lupus. J Immunol. 2012;189:3741-50 pubmed
Ryzhov S, Novitskiy S, Goldstein A, Biktasova A, Blackburn M, Biaggioni I, et al. Adenosinergic regulation of the expansion and immunosuppressive activity of CD11b+Gr1+ cells. J Immunol. 2011;187:6120-9 pubmed publisher
Ng L, Qin J, Roediger B, Wang Y, Jain R, Cavanagh L, et al. Visualizing the neutrophil response to sterile tissue injury in mouse dermis reveals a three-phase cascade of events. J Invest Dermatol. 2011;131:2058-68 pubmed publisher
Fujita M, Kohanbash G, Fellows Mayle W, Hamilton R, Komohara Y, Decker S, et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res. 2011;71:2664-74 pubmed publisher
Guiducci C, Tripodo C, Gong M, Sangaletti S, Colombo M, Coffman R, et al. Autoimmune skin inflammation is dependent on plasmacytoid dendritic cell activation by nucleic acids via TLR7 and TLR9. J Exp Med. 2010;207:2931-42 pubmed publisher
Sumagin R, Prizant H, Lomakina E, Waugh R, Sarelius I. LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ. J Immunol. 2010;185:7057-66 pubmed publisher
Tadagavadi R, REEVES W. Endogenous IL-10 attenuates cisplatin nephrotoxicity: role of dendritic cells. J Immunol. 2010;185:4904-11 pubmed publisher
Daley J, Thomay A, Connolly M, Reichner J, Albina J. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol. 2008;83:64-70 pubmed
product information
Antigen :
Ly-6G
Apps. Abbrev. :
FC, IHC
Cat # :
127603
Clone :
1A8
Item :
Biotin anti-mouse Ly-6G
Isotype :
Rat IgG2a, κ
Other Names :
Lymphocyte antigen 6 complex, locus G
Size :
50 μg
Price (USD) :
77 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BIOTIN
Immunogen :
Ly-6G transfected EL-4J cell line.
Application Notes :
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^15. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^16.Additional reported applications (for the relevant formats) include: immunohistochemistry^9 of frozen sections^10 and paraffin-embedded sections^11, depletion^4, 12-14, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA