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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse CD274 (B7-H1, PD-L1)
catalog :
124315
quantity :
125 μL
price :
204 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
10F.9G2
reactivity :
mouse
application :
western blot, flow cytometry
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • western blot; mouse; 1:100; loading ...; fig 6c
Hsu J, Xia W, Hsu Y, Chan L, Yu W, Cha J, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018;9:1908 pubmed publisher
  • flow cytometry; mouse; 1:800; loading ...; fig 3a
Starobinets H, Ye J, Broz M, Barry K, Goldsmith J, Marsh T, et al. Antitumor adaptive immunity remains intact following inhibition of autophagy and antimalarial treatment. J Clin Invest. 2016;126:4417-4429 pubmed publisher
Vogel A, Martin K, Soukup K, Halfmann A, Kerndl M, Brunner J, et al. JAK1 signaling in dendritic cells promotes peripheral tolerance in autoimmunity through PD-L1-mediated regulatory T cell induction. Cell Rep. 2022;38:110420 pubmed publisher
Walsh S, Sheridan R, Lucas E, Doan T, Ware B, Schafer J, et al. Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node. elife. 2021;10: pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
Lucas E, Schafer J, Matsuda J, Kraus M, Burchill M, Tamburini B. PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity. Cell Rep. 2020;33:108258 pubmed publisher
Chitu V, Biundo F, Shlager G, Park E, Wang P, Gulinello M, et al. Microglial Homeostasis Requires Balanced CSF-1/CSF-2 Receptor Signaling. Cell Rep. 2020;30:3004-3019.e5 pubmed publisher
Mansouri S, Katikaneni D, Gogoi H, Pipkin M, Machuca T, Emtiazjoo A, et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state. Mucosal Immunol. 2020;13:595-608 pubmed publisher
Yu D, Liu X, Han G, Liu Y, Zhao X, Wang D, et al. The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation. Cell Commun Signal. 2019;17:173 pubmed publisher
Lai N, Musser M, Pinho Ribeiro F, Baral P, Jacobson A, Ma P, et al. Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense. Cell. 2020;180:33-49.e22 pubmed publisher
Zhao Y, Lee C, Lin C, Gassen R, Xu X, Huang Z, et al. PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways. Immunity. 2019;51:1059-1073.e9 pubmed publisher
Uluçkan O, Jimenez M, Roediger B, Schnabl J, Díez Córdova L, Troulé K, et al. Cutaneous Immune Cell-Microbiota Interactions Are Controlled by Epidermal JunB/AP-1. Cell Rep. 2019;29:844-859.e3 pubmed publisher
Bommareddy P, Lowe D, Kaufman H, Rabkin S, Saha D. Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma. J Biol Methods. 2019;6: pubmed
Rosato P, Wijeyesinghe S, Stolley J, Nelson C, Davis R, Manlove L, et al. Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy. Nat Commun. 2019;10:567 pubmed publisher
Wu B, Sun X, Gupta H, Yuan B, Li J, Ge F, et al. Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer. Oncoimmunology. 2018;7:e1500107 pubmed publisher
O Reilly L, Putoczki T, Mielke L, Low J, Lin A, Preaudet A, et al. Loss of NF-κB1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner. Immunity. 2018;48:570-583.e8 pubmed publisher
Best S, De Souza D, Kersbergen A, Policheni A, Dayalan S, Tull D, et al. Synergy between the KEAP1/NRF2 and PI3K Pathways Drives Non-Small-Cell Lung Cancer with an Altered Immune Microenvironment. Cell Metab. 2018;27:935-943.e4 pubmed publisher
Saha D, Martuza R, Rabkin S. Macrophage Polarization Contributes to Glioblastoma Eradication by Combination Immunovirotherapy and Immune Checkpoint Blockade. Cancer Cell. 2017;32:253-267.e5 pubmed publisher
van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
Jiao S, Xia W, Yamaguchi H, Wei Y, Chen M, Hsu J, et al. PARP Inhibitor Upregulates PD-L1 Expression and Enhances Cancer-Associated Immunosuppression. Clin Cancer Res. 2017;23:3711-3720 pubmed publisher
Lei G, Zhang C, Lee C. Myeloid-derived suppressor cells impair alveolar macrophages through PD-1 receptor ligation during Pneumocystis pneumonia. Infect Immun. 2015;83:572-82 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
Scarlett U, Rutkowski M, Rauwerdink A, Fields J, Escovar Fadul X, Baird J, et al. Ovarian cancer progression is controlled by phenotypic changes in dendritic cells. J Exp Med. 2012;209:495-506 pubmed publisher
Meng Q, Yang P, Li B, Zhou H, Huang X, Zhu L, et al. CD4+PD-1+ T cells acting as regulatory cells during the induction of anterior chamber-associated immune deviation. Invest Ophthalmol Vis Sci. 2006;47:4444-52 pubmed
product information
Antigen :
CD274
Apps. Abbrev. :
Flow Cytometry
Cat # :
124315
Clone :
10F.9G2
Item :
Brilliant Violet 421™ anti-mouse CD274 (B7-H1, PD-L1)
Isotype :
Rat IgG2b, κ
Other Names :
B7-H1, PD-L1
Size :
125 μL
Price (USD) :
204 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Application Notes :
Additional reported applications (for the relevant formats) include: immunofluorescence 4 , blocking 6,7,8,9 , and immunohistochemistry of acetone-fixed frozen sections 4, 11 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA