product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse/rat/human CD27
catalog :
124202
quantity :
500 μg
price :
258 USD
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
LG.3A10
reactivity :
human, mouse, rat
application :
flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • mass cytometry; mouse; loading ...
Joseph R, Soundararajan R, Vasaikar S, Yang F, Allton K, Tian L, et al. CD8+ T cells inhibit metastasis and CXCL4 regulates its function. Br J Cancer. 2021;125:176-189 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • mass cytometry; mouse; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6d
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Lino C, Barros Martins J, Oberdörfer L, Walzer T, Prinz I. Eomes expression reports the progressive differentiation of IFN-?-producing Th1-like ?? T cells. Eur J Immunol. 2017;47:970-981 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1a
Ohs I, Van Den Broek M, Nussbaum K, MUNZ C, Arnold S, Quezada S, et al. Interleukin-12 bypasses common gamma-chain signalling in emergency natural killer cell lymphopoiesis. Nat Commun. 2016;7:13708 pubmed publisher
  • flow cytometry; human; fig 2a
Chung B, Guevel B, Reynolds G, Gupta Udatha D, Henriksen E, Stamataki Z, et al. Phenotyping and auto-antibody production by liver-infiltrating B cells in primary sclerosing cholangitis and primary biliary cholangitis. J Autoimmun. 2017;77:45-54 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Kwong Chung C, Ronchi F, Geuking M. Detrimental effect of systemic antimicrobial CD4+ T-cell reactivity on gut epithelial integrity. Immunology. 2017;150:221-235 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, et al. The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. J Exp Med. 2016;213:2437-2457 pubmed
  • flow cytometry; mouse; loading ...; fig 2g
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • flow cytometry; mouse; fig 2
Verma S, Weiskopf D, Gupta A, McDonald B, Peters B, Sette A, et al. Cytomegalovirus-Specific CD4 T Cells Are Cytolytic and Mediate Vaccine Protection. J Virol. 2016;90:650-8 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Schulz A, Mälzer J, Domingo C, Jürchott K, Grützkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Littwitz Salomon E, Akhmetzyanova I, Vallet C, Francois S, Dittmer U, Gibbert K. Activated regulatory T cells suppress effector NK cell responses by an IL-2-mediated mechanism during an acute retroviral infection. Retrovirology. 2015;12:66 pubmed publisher
  • flow cytometry; mouse; fig 3b
Li Y, Shen C, Zhu B, Shi F, Eisen H, Chen J. Persistent Antigen and Prolonged AKT-mTORC1 Activation Underlie Memory CD8 T Cell Impairment in the Absence of CD4 T Cells. J Immunol. 2015;195:1591-8 pubmed publisher
  • flow cytometry; mouse; tbl 1
Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, et al. Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice. J Neuroinflammation. 2015;12:208 pubmed publisher
  • flow cytometry; mouse
Martin P, Dubois C, Jacquier E, Dion S, Mancini Bourgine M, Godon O, et al. TG1050, an immunotherapeutic to treat chronic hepatitis B, induces robust T cells and exerts an antiviral effect in HBV-persistent mice. Gut. 2015;64:1961-71 pubmed publisher
  • flow cytometry; mouse; fig 1
Mehta P, Nuotio Antar A, Smith C. γδ T cells promote inflammation and insulin resistance during high fat diet-induced obesity in mice. J Leukoc Biol. 2015;97:121-34 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c
McKinstry K, Strutt T, Bautista B, Zhang W, Kuang Y, Cooper A, et al. Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2. Nat Commun. 2014;5:5377 pubmed publisher
  • flow cytometry; human
Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
Wu R, Ohara R, Jo S, Liu T, Ferris S, Ou F, et al. Mechanisms of CD40-dependent cDC1 licensing beyond costimulation. Nat Immunol. 2022;23:1536-1550 pubmed publisher
Liu X, Yu J, Xu L, Umphred Wilson K, Peng F, Ding Y, et al. Notch-induced endoplasmic reticulum-associated degradation governs mouse thymocyte β-selection. elife. 2021;10: pubmed publisher
Mouat I, Morse Z, Shanina I, Brown K, Horwitz M. Latent gammaherpesvirus exacerbates arthritis through modification of age-associated B cells. elife. 2021;10: pubmed publisher
Deng Y, Chatterjee B, Zens K, Zdimerova H, Muller A, Schuhmachers P, et al. CD27 is required for protective lytic EBV antigen-specific CD8+ T-cell expansion. Blood. 2021;137:3225-3236 pubmed publisher
Tao H, Li L, Liao N, Schluns K, Luckhart S, Sleasman J, et al. Thymic Epithelial Cell-Derived IL-15 and IL-15 Receptor α Chain Foster Local Environment for Type 1 Innate Like T Cell Development. Front Immunol. 2021;12:623280 pubmed publisher
Cytlak U, Resteu A, Pagan S, Green K, Milne P, Maisuria S, et al. Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans. Immunity. 2020;53:353-370.e8 pubmed publisher
Álvarez Salamero C, Castillo González R, Pastor Fernández G, Mariblanca I, Pino J, Cibrian D, et al. IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biol. 2020;18:e3000646 pubmed publisher
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
Dienz O, Devault V, Musial S, Mistri S, Mei L, Baraev A, et al. Critical Role for SLAM/SAP Signaling in the Thymic Developmental Programming of IL-17- and IFN-γ-Producing γδ T Cells. J Immunol. 2020;204:1521-1534 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Crowell P, Fox J, Hashimoto T, Diaz J, Navarro H, Henry G, et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep. 2019;28:1499-1510.e6 pubmed publisher
Wei S, Sharma R, Anang N, Levine J, Zhao Y, Mancuso J, et al. Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States. Immunity. 2019;50:1084-1098.e10 pubmed publisher
Klein F, Mitrovic M, Roux J, Engdahl C, von Muenchow L, Albertí Servera L, et al. The transcription factor Duxbl mediates elimination of pre-T cells that fail β-selection. J Exp Med. 2019;216:638-655 pubmed publisher
Wilgenburg B, Loh L, Chen Z, Pediongco T, Wang H, Shi M, et al. MAIT cells contribute to protection against lethal influenza infection in vivo. Nat Commun. 2018;9:4706 pubmed publisher
Shehata H, Khan S, Chen E, Fields P, Flavell R, Sanjabi S. Lack of Sprouty 1 and 2 enhances survival of effector CD8+ T cells and yields more protective memory cells. Proc Natl Acad Sci U S A. 2018;115:E8939-E8947 pubmed publisher
Goltsev Y, Samusik N, Kennedy Darling J, Bhate S, Hale M, Vazquez G, et al. Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging. Cell. 2018;174:968-981.e15 pubmed publisher
Andargachew R, Martinez R, Kolawole E, Evavold B. CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection. J Immunol. 2018;201:19-30 pubmed publisher
Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
Wei S, Levine J, Cogdill A, Zhao Y, Anang N, Andrews M, et al. Distinct Cellular Mechanisms Underlie Anti-CTLA-4 and Anti-PD-1 Checkpoint Blockade. Cell. 2017;170:1120-1133.e17 pubmed publisher
Nussbaum K, Burkhard S, Ohs I, Mair F, Klose C, Arnold S, et al. Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs. J Exp Med. 2017;214:2331-2347 pubmed publisher
Kapnick S, Stinchcombe J, Griffiths G, Schwartzberg P. Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8+ CTLs. J Immunol. 2017;198:2699-2711 pubmed publisher
Riether C, Schürch C, Bührer E, Hinterbrandner M, Huguenin A, Hoepner S, et al. CD70/CD27 signaling promotes blast stemness and is a viable therapeutic target in acute myeloid leukemia. J Exp Med. 2017;214:359-380 pubmed publisher
Li M, Bozzacco L, Hoffmann H, Breton G, Loschko J, Xiao J, et al. Interferon regulatory factor 2 protects mice from lethal viral neuroinvasion. J Exp Med. 2016;213:2931-2947 pubmed
White C, Pone E, Lam T, Tat C, Hayama K, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50 pubmed publisher
Vicetti Miguel R, Hendricks R, Aguirre A, Melan M, Harvey S, Terry Allison T, et al. Dendritic cell activation and memory cell development are impaired among mice administered medroxyprogesterone acetate prior to mucosal herpes simplex virus type 1 infection. J Immunol. 2012;189:3449-61 pubmed
Welner R, Esplin B, Garrett K, Pelayo R, Luche H, Fehling H, et al. Asynchronous RAG-1 expression during B lymphopoiesis. J Immunol. 2009;183:7768-77 pubmed publisher
product information
Antigen :
CD27
Apps. Abbrev. :
FC, IHC-F
Cat # :
124202
Clone :
LG.3A10
Item :
Purified anti-mouse/rat/human CD27
Isotype :
Armenian Hamster IgG
Other Names :
T14, S152, Tp55, TNFRSF7
Size :
500 μg
Price (USD) :
258 USD
Reactivity :
Mouse, Rat, Human
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PURE
Immunogen :
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Application Notes :
Additional reported applications (for the relevant formats) include: functional assay^1,3 and immunohistomecial staining of acetone-fixed frozen sections^2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA