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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
catalog :
122514
quantity :
100 μg
price :
292 USD
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
E13-161.7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s2a
Nagai M, Noguchi R, Takahashi D, Morikawa T, Koshida K, Komiyama S, et al. Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal Immune Responses. Cell. 2019;178:1072-1087.e14 pubmed publisher
  • flow cytometry; mouse; fig 1a
Mitroulis I, Ruppova K, Wang B, Chen L, Grzybek M, Grinenko T, et al. Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell. 2018;172:147-161.e12 pubmed publisher
  • flow cytometry; mouse; fig 7
Sinclair A, Park L, Shah M, Drotar M, Calaminus S, Hopcroft L, et al. CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood. 2016;128:371-83 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8a
Griffiths K, Dolezal O, Cao B, Nilsson S, See H, Pfleger K, et al. i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016;291:12641-57 pubmed publisher
Murakami K, Kurotaki D, Kawase W, Soma S, Fukuchi Y, Kunimoto H, et al. OGT Regulates Hematopoietic Stem Cell Maintenance via PINK1-Dependent Mitophagy. Cell Rep. 2021;34:108579 pubmed publisher
Lefkopoulos S, Polyzou A, Derecka M, Bergo V, Clapes T, Cauchy P, et al. Repetitive Elements Trigger RIG-I-like Receptor Signaling that Regulates the Emergence of Hematopoietic Stem and Progenitor Cells. Immunity. 2020;53:934-951.e9 pubmed publisher
Kalafati L, Kourtzelis I, Schulte Schrepping J, Li X, Hatzioannou A, Grinenko T, et al. Innate Immune Training of Granulopoiesis Promotes Anti-tumor Activity. Cell. 2020;183:771-785.e12 pubmed publisher
Kobayashi H, Takubo K. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protoc. 2020;1:100078 pubmed publisher
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
Sztwiertnia I, Schenz J, Bomans K, Schaack D, Ohnesorge J, Tamulyte S, et al. Sevoflurane depletes macrophages from the melanoma microenvironment. PLoS ONE. 2020;15:e0233789 pubmed publisher
Cheng Y, Luo H, Izzo F, Pickering B, Nguyen D, Myers R, et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep. 2019;28:1703-1716.e6 pubmed publisher
Kobayashi H, Morikawa T, Okinaga A, Hamano F, Hashidate Yoshida T, Watanuki S, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28:145-158.e9 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Muzumdar S, Hiebert H, Haertel E, Ben Yehuda Greenwald M, Bloch W, Werner S, et al. Nrf2-Mediated Expansion of Pilosebaceous Cells Accelerates Cutaneous Wound Healing. Am J Pathol. 2019;189:568-579 pubmed publisher
Yamamoto R, Wilkinson A, Ooehara J, Lan X, Lai C, Nakauchi Y, et al. Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment. Cell Stem Cell. 2018;22:600-607.e4 pubmed publisher
Guo B, Huang X, Lee M, Lee S, Broxmeyer H. Antagonism of PPAR-γ signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nat Med. 2018;24:360-367 pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Rossnagl S, Altrock E, Sens C, Kraft S, Rau K, Milsom M, et al. EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. PLoS Biol. 2016;14:e1002562 pubmed publisher
Fournier M, Lebert Ghali C, Bijl J. HOXA4 provides stronger engraftment potential to short-term repopulating cells than HOXB4. Stem Cells Dev. 2015;24:2413-22 pubmed publisher
Chung Y, Kim E, Abdel Wahab O. Femoral bone marrow aspiration in live mice. J Vis Exp. 2014;: pubmed publisher
Nihon Yanagi Y, Terai K, Murano T, Kawai T, Kimura S, Okazumi S. ?-2 microglobulin is unsuitable as an internal reference gene for the analysis of gene expression in human colorectal cancer. Biomed Rep. 2013;1:193-196 pubmed
Hara T, Shitara S, Imai K, Miyachi H, Kitano S, Yao H, et al. Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. J Immunol. 2012;189:1577-84 pubmed publisher
Rosas M, Thomas B, Stacey M, Gordon S, Taylor P. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol. 2010;88:169-80 pubmed publisher
Felthaus O, Ernst W, Driemel O, Reichert T, Schmalz G, Morsczeck C. TGF-beta stimulates glial-like differentiation in murine dental follicle precursor cells (mDFPCs). Neurosci Lett. 2010;471:179-84 pubmed publisher
product information
Antigen :
Ly-6A Ly-6E
Apps. Abbrev. :
Flow Cytometry
Cat # :
122514
Clone :
E13-161.7
Item :
PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
100 μg
Price (USD) :
292 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
mouse pre-T cells
Application Notes :
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity 1 , immunohistochemical staining of frozen sections 2 and Bouin-fixed, paraffin-embedded samples 4 , immunofluorescence microscopy 4 , and immunoprecipitation 3 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 7 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA