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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse Ly-6A/E (Sca-1)
catalog :
122501
quantity :
50 μg
price :
55 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
E13-161.7
reactivity :
human, mouse
application :
immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4d
Ray S, Chee L, Matson D, Palermo N, Bresnick E, Hewitt K. Sterile α-motif domain requirement for cellular signaling and survival. J Biol Chem. 2020;295:7113-7125 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Rao T, Gupta M, Softic S, Wang L, Jang Y, Thomou T, et al. Attenuation of PKCδ enhances metabolic activity and promotes expansion of blood progenitors. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2a
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Umemoto T, Hashimoto M, Matsumura T, Nakamura Ishizu A, Suda T. Ca2+-mitochondria axis drives cell division in hematopoietic stem cells. J Exp Med. 2018;215:2097-2113 pubmed publisher
  • flow cytometry; mouse; loading ...
Forster M, Farrington K, Petrov J, Belle J, Mindt B, Witalis M, et al. MYSM1-dependent checkpoints in B cell lineage differentiation and B cell-mediated immune response. J Leukoc Biol. 2017;101:643-654 pubmed publisher
  • flow cytometry; human; loading ...; fig 5
Kotschy A, Szlávik Z, Murray J, Davidson J, Maragno A, Le Toumelin Braizat G, et al. The MCL1 inhibitor S63845 is tolerable and effective in diverse cancer models. Nature. 2016;538:477-482 pubmed publisher
  • immunocytochemistry; mouse; fig 2
Lopes C, Daifalla N, Das B, Dias da Silva V, Campos Neto A. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum. PLoS ONE. 2016;11:e0162927 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Vogel K, Bell L, Galloway A, Ahlfors H, Turner M. The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic ?-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression. J Immunol. 2016;197:2673-2685 pubmed publisher
  • flow cytometry; mouse; tbl s3
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • flow cytometry; mouse; fig s3
Hu W, Dooley J, Chung S, Chandramohan D, Cimmino L, Mukherjee S, et al. miR-29a maintains mouse hematopoietic stem cell self-renewal by regulating Dnmt3a. Blood. 2015;125:2206-16 pubmed publisher
  • flow cytometry; mouse
CismaÅŸiu V, Popescu L. Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells. J Cell Mol Med. 2015;19:351-8 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
Goldsmith J, Spitofsky N, Zamani A, Hood R, Boggs A, Li X, et al. TNFAIP8 controls murine intestinal stem cell homeostasis and regeneration by regulating microbiome-induced Akt signaling. Nat Commun. 2020;11:2591 pubmed publisher
Shamsi F, Xue R, Huang T, Lundh M, Liu Y, Leiria L, et al. FGF6 and FGF9 regulate UCP1 expression independent of brown adipogenesis. Nat Commun. 2020;11:1421 pubmed publisher
Helbling P, Piñeiro Yañez E, Gerosa R, Boettcher S, Al Shahrour F, Manz M, et al. Global Transcriptomic Profiling of the Bone Marrow Stromal Microenvironment during Postnatal Development, Aging, and Inflammation. Cell Rep. 2019;29:3313-3330.e4 pubmed publisher
Matsuyama H, Isshiki T, Chiba A, Yamaguchi T, Murayama G, Akasaka Y, et al. Activation of mucosal-associated invariant T cells in the lungs of sarcoidosis patients. Sci Rep. 2019;9:13181 pubmed publisher
Zhu W, Naler L, Maul R, Sallin M, Sen J. Immune system development and age-dependent maintenance in Klotho-hypomorphic mice. Aging (Albany NY). 2019;11:5246-5257 pubmed publisher
Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
Sala D, Cunningham T, Stec M, Etxaniz U, Nicoletti C, Dall Agnese A, et al. The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration. Nat Commun. 2019;10:1796 pubmed publisher
Dey A, Yang W, Gegonne A, Nishiyama A, Pan R, Yagi R, et al. BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses. EMBO J. 2019;38: pubmed publisher
Ishii H, Vodnala S, Achyut B, So J, Hollander M, Greten T, et al. miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity. Nat Commun. 2018;9:2611 pubmed publisher
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
Zhang H, Li H, Hillmer E, Zhao Y, Chrisikos T, Hu H, et al. Genetic rescue of lineage-balanced blood cell production reveals a crucial role for STAT3 antiinflammatory activity in hematopoiesis. Proc Natl Acad Sci U S A. 2018;115:E2311-E2319 pubmed publisher
Chartrand K, Lebel M, Tarrab E, Savard P, Leclerc D, Lamarre A. Efficacy of a Virus-Like Nanoparticle As Treatment for a Chronic Viral Infection Is Hindered by IRAK1 Regulation and Antibody Interference. Front Immunol. 2017;8:1885 pubmed publisher
Zhang C, Yi W, Li F, Du X, Wang H, Wu P, et al. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis. Cell Res. 2018;28:323-335 pubmed publisher
Martin K, Pederzoli Ribeil M, Pacreau E, Burgener S, Dahdah A, Candalh C, et al. Transgenic Mice Expressing Human Proteinase 3 Exhibit Sustained Neutrophil-Associated Peritonitis. J Immunol. 2017;199:3914-3924 pubmed publisher
Gronowicz G, Jacobs E, Peng T, Zhu L, Hurley M, Kuhn L. * Calvarial Bone Regeneration Is Enhanced by Sequential Delivery of FGF-2 and BMP-2 from Layer-by-Layer Coatings with a Biomimetic Calcium Phosphate Barrier Layer. Tissue Eng Part A. 2017;23:1490-1501 pubmed publisher
Kollek M, Voigt G, Molnar C, Murad F, Bertele D, Krombholz C, et al. Transient apoptosis inhibition in donor stem cells improves hematopoietic stem cell transplantation. J Exp Med. 2017;214:2967-2983 pubmed publisher
Rogers P, Driessnack M, Hiltbold Schwartz E. Analysis of the developmental stages, kinetics, and phenotypes exhibited by myeloid cells driven by GM-CSF in vitro. PLoS ONE. 2017;12:e0181985 pubmed publisher
Umemoto T, Matsuzaki Y, Shiratsuchi Y, Hashimoto M, Yoshimoto T, Nakamura Ishizu A, et al. Integrin αvβ3 enhances the suppressive effect of interferon-γ on hematopoietic stem cells. EMBO J. 2017;36:2390-2403 pubmed publisher
Ono R, Masuya M, Ishii S, Katayama N, Nosaka T. Eya2, a Target Activated by Plzf, Is Critical for PLZF-RARA-Induced Leukemogenesis. Mol Cell Biol. 2017;37: pubmed publisher
Nascimento D, Melo P, Piñeros A, Ferreira R, Cólon D, Donate P, et al. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population. Nat Commun. 2017;8:14919 pubmed publisher
Lujan E, Zunder E, Ng Y, Goronzy I, Nolan G, Wernig M. Early reprogramming regulators identified by prospective isolation and mass cytometry. Nature. 2015;521:352-6 pubmed publisher
Rosas M, Thomas B, Stacey M, Gordon S, Taylor P. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol. 2010;88:169-80 pubmed publisher
Felthaus O, Ernst W, Driemel O, Reichert T, Schmalz G, Morsczeck C. TGF-beta stimulates glial-like differentiation in murine dental follicle precursor cells (mDFPCs). Neurosci Lett. 2010;471:179-84 pubmed publisher
Brandl C, Florian C, Driemel O, Weber B, Morsczeck C. Identification of neural crest-derived stem cell-like cells from the corneal limbus of juvenile mice. Exp Eye Res. 2009;89:209-17 pubmed publisher
product information
Antigen :
Ly-6A Ly-6E
Apps. Abbrev. :
Flow Cytometry, IHC-F, IHC-P, IP
Cat # :
122501
Clone :
E13-161.7
Item :
Purified anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
50 μg
Price (USD) :
55 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
mouse pre-T cells
Application Notes :
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity 1 , immunohistochemical staining of frozen sections 2 and Bouin-fixed, paraffin-embedded samples 4 , immunofluorescence microscopy 4 , and immunoprecipitation 3 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 7
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA