product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM)
catalog :
118211
quantity :
25 μg
price :
124 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
G8.8
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:500; loading ...; fig s2a, s2d
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...
Kurashima Y, Kigoshi T, Murasaki S, Arai F, Shimada K, Seki N, et al. Pancreatic glycoprotein 2 is a first line of defense for mucosal protection in intestinal inflammation. Nat Commun. 2021;12:1067 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 6a
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s20
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s1a
Wells K, Miller C, Gschwind A, Wei W, Phipps J, Anderson M, et al. Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2b, 2c
Mevel R, Steiner I, Mason S, Galbraith L, Patel R, Fadlullah M, et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s1-1a
Tremblay M, Viala S, Shafer M, Graham Paquin A, Liu C, Bouchard M. Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 2 ug/ml; loading ...; fig s9a, s25b, s25e
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; human; loading ...
  • immunocytochemistry; human; loading ...; fig 4a
Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, et al. CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing. Cell Mol Gastroenterol Hepatol. 2020;10:101-112 pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Shikama Y, Kurosawa M, Furukawa M, Ishimaru N, Matsushita K. Involvement of adiponectin in age-related increases in tear production in mice. Aging (Albany NY). 2019;11:8329-8346 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2s2c
Katsuda T, Matsuzaki J, Yamaguchi T, Yamada Y, Prieto Vila M, Hosaka K, et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6e
Hendrikx S, Coso S, Prat Luri B, Wetterwald L, Sabine A, Franco C, et al. Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2. Cell Rep. 2019;26:1227-1241.e6 pubmed publisher
  • flow cytometry; mouse; fig 2a
James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2
Jürgensen H, Nørregaard K, Sibree M, Santoni Rugiu E, Madsen D, Wassilew K, et al. Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins. J Cell Biol. 2019;218:333-349 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c
Capucha T, Koren N, Nassar M, Heyman O, Nir T, Levy M, et al. Sequential BMP7/TGF-β1 signaling and microbiota instruct mucosal Langerhans cell differentiation. J Exp Med. 2018;215:481-500 pubmed publisher
  • immunohistochemistry - frozen section; human; 5 ug/ml; loading ...; fig s1f
Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s2k
Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
  • flow cytometry; mouse; fig 1a
Engelmann R, Biemelt A, Cordshagen A, Johl A, Kuthning D, Müller Hilke B. The Prerequisites for Central Tolerance Induction against Citrullinated Proteins in the Mouse. PLoS ONE. 2016;11:e0158773 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile. J Immunol. 2016;196:4760-70 pubmed publisher
  • flow cytometry; mouse; fig s1
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, et al. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol. 2015;194:307-15 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Paiva R, Sousa A, Ramos C, Avila M, Lilue J, Paixão T, et al. Self-renewal of double-negative 3 early thymocytes enables thymus autonomy but compromises the β-selection checkpoint. Cell Rep. 2021;35:108967 pubmed publisher
Moon S, Park Y, Hyeon S, Kim Y, Kim J, Kim H, et al. Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation. J Exp Med. 2021;218: pubmed publisher
Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo C, et al. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue. Cell. 2020;183:1665-1681.e18 pubmed publisher
Tang R, Murray C, Linde I, Kramer N, Lyu Z, Tsai M, et al. A versatile system to record cell-cell interactions. elife. 2020;9: pubmed publisher
Bilate A, London M, Castro T, Mesin L, Bortolatto J, Kongthong S, et al. T Cell Receptor Is Required for Differentiation, but Not Maintenance, of Intestinal CD4+ Intraepithelial Lymphocytes. Immunity. 2020;53:1001-1014.e20 pubmed publisher
Gabitova Cornell L, Surumbayeva A, Peri S, Franco Barraza J, Restifo D, Weitz N, et al. Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer. Cancer Cell. 2020;38:567-583.e11 pubmed publisher
Strunz M, Simon L, Ansari M, Kathiriya J, Angelidis I, Mayr C, et al. Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis. Nat Commun. 2020;11:3559 pubmed publisher
Madenspacher J, Morrell E, Gowdy K, McDonald J, Thompson B, Muse G, et al. Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation. JCI Insight. 2020;5: pubmed publisher
Cheng Q, Wei T, Farbiak L, Johnson L, Dilliard S, Siegwart D. Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR-Cas gene editing. Nat Nanotechnol. 2020;15:313-320 pubmed publisher
Satoh Takayama N, Kato T, Motomura Y, Kageyama T, Taguchi Atarashi N, Kinoshita Daitoku R, et al. Bacteria-Induced Group 2 Innate Lymphoid Cells in the Stomach Provide Immune Protection through Induction of IgA. Immunity. 2020;52:635-649.e4 pubmed publisher
Bernstock J, Totten A, Elkahloun A, Johnson K, Hurst A, Goldman F, et al. Recurrent microdeletions at chromosome 2p11.2 are associated with thymic hypoplasia and features resembling DiGeorge syndrome. J Allergy Clin Immunol. 2020;145:358-367.e2 pubmed publisher
Chung C, Ma Z, Dravis C, Preissl S, Poirion O, Luna G, et al. Single-Cell Chromatin Analysis of Mammary Gland Development Reveals Cell-State Transcriptional Regulators and Lineage Relationships. Cell Rep. 2019;29:495-510.e6 pubmed publisher
Koyama M, Mukhopadhyay P, Schuster I, Henden A, Hülsdünker J, Varelias A, et al. MHC Class II Antigen Presentation by the Intestinal Epithelium Initiates Graft-versus-Host Disease and Is Influenced by the Microbiota. Immunity. 2019;51:885-898.e7 pubmed publisher
Joshi P, Waterhouse P, Kasaian K, Fang H, Gulyaeva O, Sul H, et al. PDGFRα+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland. Nat Commun. 2019;10:1760 pubmed publisher
Riemondy K, Jansing N, Jiang P, Redente E, Gillen A, Fu R, et al. Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury. JCI Insight. 2019;5: pubmed publisher
Biffi G, Oni T, Spielman B, Hao Y, Elyada E, Park Y, et al. IL1-Induced JAK/STAT Signaling Is Antagonized by TGFβ to Shape CAF Heterogeneity in Pancreatic Ductal Adenocarcinoma. Cancer Discov. 2019;9:282-301 pubmed publisher
Dravis C, Chung C, Lytle N, Herrera Valdez J, Luna G, Trejo C, et al. Epigenetic and Transcriptomic Profiling of Mammary Gland Development and Tumor Models Disclose Regulators of Cell State Plasticity. Cancer Cell. 2018;34:466-482.e6 pubmed publisher
Cohen S, Gern B, Delahaye J, Adams K, Plumlee C, Winkler J, et al. Alveolar Macrophages Provide an Early Mycobacterium tuberculosis Niche and Initiate Dissemination. Cell Host Microbe. 2018;24:439-446.e4 pubmed publisher
Giraddi R, Chung C, Heinz R, Balcioglu O, Novotny M, Trejo C, et al. Single-Cell Transcriptomes Distinguish Stem Cell State Changes and Lineage Specification Programs in Early Mammary Gland Development. Cell Rep. 2018;24:1653-1666.e7 pubmed publisher
Campbell C, Dikiy S, Bhattarai S, Chinen T, Matheis F, Calafiore M, et al. Extrathymically Generated Regulatory T Cells Establish a Niche for Intestinal Border-Dwelling Bacteria and Affect Physiologic Metabolite Balance. Immunity. 2018;48:1245-1257.e9 pubmed publisher
Zhang J, Burn C, Young K, Wilson M, Ly K, Budhwani M, et al. Microparticles produced by human papillomavirus type 16 E7-expressing cells impair antigen presenting cell function and the cytotoxic T cell response. Sci Rep. 2018;8:2373 pubmed publisher
Miragaia R, Zhang X, Gomes T, Svensson V, Ilicic T, Henriksson J, et al. Single-cell RNA-sequencing resolves self-antigen expression during mTEC development. Sci Rep. 2018;8:685 pubmed publisher
Burns K, Thomas S, Hamilton K, Young S, Cook D, Korach K. Early Endometriosis in Females Is Directed by Immune-Mediated Estrogen Receptor α and IL-6 Cross-Talk. Endocrinology. 2018;159:103-118 pubmed publisher
McClendon J, Jansing N, Redente E, Gandjeva A, Ito Y, Colgan S, et al. Hypoxia-Inducible Factor 1? Signaling Promotes Repair of the Alveolar Epithelium after Acute Lung Injury. Am J Pathol. 2017;187:1772-1786 pubmed publisher
van der Heijden M, Zimberlin C, Nicholson A, Colak S, Kemp R, Meijer S, et al. Bcl-2 is a critical mediator of intestinal transformation. Nat Commun. 2016;7:10916 pubmed publisher
Giraddi R, Shehata M, Gallardo M, Blasco M, Simons B, Stingl J. Stem and progenitor cell division kinetics during postnatal mouse mammary gland development. Nat Commun. 2015;6:8487 pubmed publisher
Wasik K, Tam O, Knott S, Falciatori I, Hammell M, Vagin V, et al. RNF17 blocks promiscuous activity of PIWI proteins in mouse testes. Genes Dev. 2015;29:1403-15 pubmed publisher
Hegab A, Arai D, Gao J, Kuroda A, Yasuda H, Ishii M, et al. Mimicking the niche of lung epithelial stem cells and characterization of several effectors of their in vitro behavior. Stem Cell Res. 2015;15:109-21 pubmed publisher
Davis F, Janoshazi A, Janardhan K, Steinckwich N, D Agostin D, Petranka J, et al. Essential role of Orai1 store-operated calcium channels in lactation. Proc Natl Acad Sci U S A. 2015;112:5827-32 pubmed publisher
Molaro A, Falciatori I, Hodges E, Aravin A, Marran K, Rafii S, et al. Two waves of de novo methylation during mouse germ cell development. Genes Dev. 2014;28:1544-9 pubmed publisher
Bretz N, Salnikov A, Doberstein K, Garbi N, Kloess V, Joumaa S, et al. Lack of CD24 expression in mice reduces the number of leukocytes in the colon. Immunol Lett. 2014;161:140-8 pubmed publisher
Hirokawa Y, Yip K, Tan C, Burgess A. Colonic myofibroblast cell line stimulates colonoid formation. Am J Physiol Gastrointest Liver Physiol. 2014;306:G547-56 pubmed publisher
Taguchi K, Hirano I, Itoh T, Tanaka M, Miyajima A, Suzuki A, et al. Nrf2 enhances cholangiocyte expansion in Pten-deficient livers. Mol Cell Biol. 2014;34:900-13 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
Ishii K, Kanatsu Shinohara M, Toyokuni S, Shinohara T. FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation. Development. 2012;139:1734-43 pubmed publisher
Takehashi M, Tada M, Kanatsu Shinohara M, Morimoto H, Kazuki Y, Oshimura M, et al. Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice. Biol Reprod. 2012;86:178 pubmed publisher
Gaiser M, Lämmermann T, Feng X, Igyarto B, Kaplan D, Tessarollo L, et al. Cancer-associated epithelial cell adhesion molecule (EpCAM; CD326) enables epidermal Langerhans cell motility and migration in vivo. Proc Natl Acad Sci U S A. 2012;109:E889-97 pubmed publisher
Morimoto H, Lee J, Tanaka T, Ishii K, Toyokuni S, Kanatsu Shinohara M, et al. In vitro transformation of mouse testis cells by oncogene transfection. Biol Reprod. 2012;86:148, 1-11 pubmed publisher
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Shaffer M, Huang Y, Corbo E, Wu G, Velez M, Choi J, et al. Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice. PLoS ONE. 2010;5:e12404 pubmed publisher
Hinterberger M, Aichinger M, Prazeres da Costa O, Voehringer D, Hoffmann R, Klein L. Autonomous role of medullary thymic epithelial cells in central CD4(+) T cell tolerance. Nat Immunol. 2010;11:512-9 pubmed publisher
Gracz A, Ramalingam S, Magness S. Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro. Am J Physiol Gastrointest Liver Physiol. 2010;298:G590-600 pubmed publisher
product information
Antigen :
CD326
Apps. Abbrev. :
Flow Cytometry, IHC-F, 3D IHC, SB
Cat # :
118211
Clone :
G8.8
Item :
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM)
Isotype :
Rat IgG2a, κ
Other Names :
CD326, EGP40, MIC18, TROP1, KSA
Size :
25 μg
Price (USD) :
124 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
TE-71 thymic epithelial cell line
Application Notes :
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed 1 , with or without OCT embedding 2,4 , and spatial biology (IBEX) 13,14 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA