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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM)
catalog :
118210
quantity :
100 μg
price :
265 USD
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
G8.8
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 51
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:500; loading ...; fig s2a, s2d
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; fig 4a, 4b, s4e
Steele N, Biffi G, Kemp S, Zhang Y, Drouillard D, Syu L, et al. Inhibition of Hedgehog Signaling Alters Fibroblast Composition in Pancreatic Cancer. Clin Cancer Res. 2021;: pubmed publisher
  • flow cytometry; mouse; 1:100; fig 6a
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s20
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s1a
Wells K, Miller C, Gschwind A, Wei W, Phipps J, Anderson M, et al. Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2b, 2c
Mevel R, Steiner I, Mason S, Galbraith L, Patel R, Fadlullah M, et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s1-1a
Tremblay M, Viala S, Shafer M, Graham Paquin A, Liu C, Bouchard M. Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 2 ug/ml; loading ...; fig s9a, s25b, s25e
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; human; loading ...
  • immunocytochemistry; human; loading ...; fig 4a
Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, et al. CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing. Cell Mol Gastroenterol Hepatol. 2020;10:101-112 pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Shikama Y, Kurosawa M, Furukawa M, Ishimaru N, Matsushita K. Involvement of adiponectin in age-related increases in tear production in mice. Aging (Albany NY). 2019;11:8329-8346 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2s2c
Katsuda T, Matsuzaki J, Yamaguchi T, Yamada Y, Prieto Vila M, Hosaka K, et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Coulombe P, Paliouras G, Clayton A, Hussainkhel A, Fuller M, Jovanovic V, et al. Endothelial Sash1 Is Required for Lung Maturation through Nitric Oxide Signaling. Cell Rep. 2019;27:1769-1780.e4 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6e
Hendrikx S, Coso S, Prat Luri B, Wetterwald L, Sabine A, Franco C, et al. Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2. Cell Rep. 2019;26:1227-1241.e6 pubmed publisher
  • flow cytometry; mouse; fig 2a
James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2
Jürgensen H, Nørregaard K, Sibree M, Santoni Rugiu E, Madsen D, Wassilew K, et al. Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins. J Cell Biol. 2019;218:333-349 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c
Capucha T, Koren N, Nassar M, Heyman O, Nir T, Levy M, et al. Sequential BMP7/TGF-β1 signaling and microbiota instruct mucosal Langerhans cell differentiation. J Exp Med. 2018;215:481-500 pubmed publisher
  • immunohistochemistry - frozen section; human; 5 ug/ml; loading ...; fig s1f
Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s2k
Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile. J Immunol. 2016;196:4760-70 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Bota Rabassedas N, Banerjee P, Niu Y, Cao W, Luo J, Xi Y, et al. Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity. Cell Rep. 2021;35:109009 pubmed publisher
Chen Y, Kim J, Yang S, Wang H, Wu C, Sugimoto H, et al. Type I collagen deletion in αSMA+ myofibroblasts augments immune suppression and accelerates progression of pancreatic cancer. Cancer Cell. 2021;39:548-565.e6 pubmed publisher
Neufert C, Heichler C, Brabletz T, Scheibe K, Boonsanay V, Greten F, et al. Inducible mouse models of colon cancer for the analysis of sporadic and inflammation-driven tumor progression and lymph node metastasis. Nat Protoc. 2021;16:61-85 pubmed publisher
Kim S, Saeidi S, Zhong X, Gwak S, Muna I, Park S, et al. Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages. Neoplasia. 2020;22:606-616 pubmed publisher
Watson J, Sanders P, Dunmore R, Rosignoli G, Jule Y, Rawlins E, et al. Distal lung epithelial progenitor cell function declines with age. Sci Rep. 2020;10:10490 pubmed publisher
Kamata T, So T, Ahmed Q, Giblett S, Patel B, Luo J, et al. Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development. Cell Rep. 2020;31:107802 pubmed publisher
Sfakianos J, Daza J, Hu Y, Anastos H, Bryant G, Bareja R, et al. Epithelial plasticity can generate multi-lineage phenotypes in human and murine bladder cancers. Nat Commun. 2020;11:2540 pubmed publisher
Fukushima K, Satoh T, Sugihara F, Sato Y, Okamoto T, Mitsui Y, et al. Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis. Immunity. 2020;52:542-556.e13 pubmed publisher
Weiner A, Jackson S, Zhao G, Quansah K, Farshchian J, Neupauer K, et al. Mesenchyme-free expansion and transplantation of adult alveolar progenitor cells: steps toward cell-based regenerative therapies. NPJ Regen Med. 2019;4:17 pubmed publisher
Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
Proietti M, Perruzza L, Scribano D, Pellegrini G, D Antuono R, Strati F, et al. ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens. Nat Commun. 2019;10:250 pubmed publisher
Pinho A, Van Bulck M, Chantrill L, Arshi M, Sklyarova T, Herrmann D, et al. ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-β signalling. Nat Commun. 2018;9:5083 pubmed publisher
Tomic G, Morrissey E, Kozar S, Ben Moshe S, Hoyle A, Azzarelli R, et al. Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration. Cell Stem Cell. 2018;23:436-443.e7 pubmed publisher
Lyons J, Ghazi P, Starchenko A, Tovaglieri A, Baldwin K, Poulin E, et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol. 2018;16:e2002417 pubmed publisher
Kojima T, Tsuchiya K, Ikemizu S, Yoshikawa S, Yamanishi Y, Watanabe M, et al. Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R. Sci Rep. 2016;6:36457 pubmed publisher
Hirokawa Y, Yip K, Tan C, Burgess A. Colonic myofibroblast cell line stimulates colonoid formation. Am J Physiol Gastrointest Liver Physiol. 2014;306:G547-56 pubmed publisher
Taguchi K, Hirano I, Itoh T, Tanaka M, Miyajima A, Suzuki A, et al. Nrf2 enhances cholangiocyte expansion in Pten-deficient livers. Mol Cell Biol. 2014;34:900-13 pubmed publisher
Ravindran A, Mohammed J, Gunderson A, Cui X, Glick A. Tumor-promoting role of TGF?1 signaling in ultraviolet B-induced skin carcinogenesis is associated with cutaneous inflammation and lymph node migration of dermal dendritic cells. Carcinogenesis. 2014;35:959-66 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
Ishii K, Kanatsu Shinohara M, Toyokuni S, Shinohara T. FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation. Development. 2012;139:1734-43 pubmed publisher
Takehashi M, Tada M, Kanatsu Shinohara M, Morimoto H, Kazuki Y, Oshimura M, et al. Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice. Biol Reprod. 2012;86:178 pubmed publisher
Morimoto H, Lee J, Tanaka T, Ishii K, Toyokuni S, Kanatsu Shinohara M, et al. In vitro transformation of mouse testis cells by oncogene transfection. Biol Reprod. 2012;86:148, 1-11 pubmed publisher
Nudel I, Elnekave M, Furmanov K, Arizon M, Clausen B, Wilensky A, et al. Dendritic cells in distinct oral mucosal tissues engage different mechanisms to prime CD8+ T cells. J Immunol. 2011;186:891-900 pubmed publisher
Hinterberger M, Aichinger M, Prazeres da Costa O, Voehringer D, Hoffmann R, Klein L. Autonomous role of medullary thymic epithelial cells in central CD4(+) T cell tolerance. Nat Immunol. 2010;11:512-9 pubmed publisher
Gracz A, Ramalingam S, Magness S. Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro. Am J Physiol Gastrointest Liver Physiol. 2010;298:G590-600 pubmed publisher
product information
Antigen :
CD326
Apps. Abbrev. :
Flow Cytometry, IHC-F, 3D IHC
Cat # :
118210
Clone :
G8.8
Item :
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM)
Isotype :
Rat IgG2a, κ
Other Names :
CD326, EGP40, MIC18, TROP1, KSA
Size :
100 μg
Price (USD) :
265 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A488
Immunogen :
TE-71 thymic epithelial cell line
Application Notes :
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed 1 , with or without OCT embedding 2,4 , and spatial biology (IBEX) 13,14 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA