product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse TCR γ/δ
catalog :
118101
quantity :
100 μg
price :
118 USD
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
GL3
reactivity :
mouse
application :
immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1a
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; mouse; 1:700; loading ...; fig s2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; 1:80; loading ...
Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Nakajima S, Tanaka R, Yamashiro K, Chiba A, Noto D, Inaba T, et al. Mucosal-Associated Invariant T Cells Are Involved in Acute Ischemic Stroke by Regulating Neuroinflammation. J Am Heart Assoc. 2021;10:e018803 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Harro C, Perez Sanz J, Costich T, Payne K, Anadon C, Chaurio R, et al. Methyltransferase inhibitors restore SATB1 protective activity against cutaneous T cell lymphoma in mice. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; 1:67; loading ...; fig s6a
Sanchez Felipe L, Vercruysse T, Sharma S, Ma J, Lemmens V, Van Looveren D, et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature. 2021;590:320-325 pubmed publisher
  • mass cytometry; mouse; 1.5 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6a
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ex5c
He S, Kahles F, Rattik S, Nairz M, McAlpine C, Anzai A, et al. Gut intraepithelial T cells calibrate metabolism and accelerate cardiovascular disease. Nature. 2019;566:115-119 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Martins J, Andoniou C, Fleming P, Kuns R, Schuster I, Voigt V, et al. Strain-specific antibody therapy prevents cytomegalovirus reactivation after transplantation. Science. 2019;363:288-293 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 6a
  • immunoprecipitation; mouse; loading ...; fig 7f
Mantri C, St John A. Immune synapses between mast cells and γδ T cells limit viral infection. J Clin Invest. 2019;129:1094-1108 pubmed publisher
  • flow cytometry; mouse; fig 2a
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Er J, Koean R, Ding J. Loss of T-bet confers survival advantage to influenza-bacterial superinfection. EMBO J. 2019;38: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d
Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4b
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Varelias A, Bunting M, Ormerod K, Koyama M, Olver S, Straube J, et al. Recipient mucosal-associated invariant T cells control GVHD within the colon. J Clin Invest. 2018;128:1919-1936 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4a
Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s12a
Sui P, Wiesner D, Xu J, Zhang Y, Lee J, Van Dyken S, et al. Pulmonary neuroendocrine cells amplify allergic asthma responses. Science. 2018;360: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Fahl S, Coffey F, Kain L, Zarin P, Dunbrack R, Teyton L, et al. Role of a selecting ligand in shaping the murine γδ-TCR repertoire. Proc Natl Acad Sci U S A. 2018;115:1889-1894 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H. γδTCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program. J Clin Invest. 2018;128:415-426 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5d
Wallrapp A, Riesenfeld S, Burkett P, Abdulnour R, Nyman J, Dionne D, et al. The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation. Nature. 2017;549:351-356 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Muschaweckh A, Petermann F, Korn T. IL-1? and IL-23 Promote Extrathymic Commitment of CD27+CD122- ?? T Cells to ??T17 Cells. J Immunol. 2017;199:2668-2679 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3c
Lis R, Karrasch C, Poulos M, Kunar B, Redmond D, Duran J, et al. Conversion of adult endothelium to immunocompetent haematopoietic stem cells. Nature. 2017;545:439-445 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7a
van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
  • flow cytometry; mouse; fig s3b
Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
  • flow cytometry; mouse; loading ...
Teng O, Chen S, Hsu T, Sia S, Cole S, Valkenburg S, et al. CLEC5A-Mediated Enhancement of the Inflammatory Response in Myeloid Cells Contributes to Influenza Virus Pathogenicity In Vivo. J Virol. 2017;91: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Hirai Yuki A, Hensley L, McGivern D, Gonzalez Lopez O, Das A, Feng H, et al. MAVS-dependent host species range and pathogenicity of human hepatitis A virus. Science. 2016;353:1541-1545 pubmed
  • flow cytometry; mouse; fig 1
Pelly V, Kannan Y, Coomes S, Entwistle L, Rückerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Lin C, Bradstreet T, Schwarzkopf E, Jarjour N, Chou C, Archambault A, et al. IL-1-induced Bhlhe40 identifies pathogenic T helper cells in a model of autoimmune neuroinflammation. J Exp Med. 2016;213:251-71 pubmed publisher
  • flow cytometry; mouse; fig 1, 2
Kim J, Choi Y, Lee B, Song M, Ban C, Kim J, et al. Programmed cell death ligand 1 alleviates psoriatic inflammation by suppressing IL-17A production from programmed cell death 1-high T cells. J Allergy Clin Immunol. 2016;137:1466-1476.e3 pubmed publisher
  • flow cytometry; mouse; fig s1
Adachi T, Kobayashi T, Sugihara E, Yamada T, Ikuta K, Pittaluga S, et al. Hair follicle-derived IL-7 and IL-15 mediate skin-resident memory T cell homeostasis and lymphoma. Nat Med. 2015;21:1272-9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Kim M, Taparowsky E, Kim C. Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut. Immunity. 2015;43:107-19 pubmed publisher
  • flow cytometry; mouse; fig 3
Imai Y, Ayithan N, Wu X, Yuan Y, Wang L, Hwang S. Cutting Edge: PD-1 Regulates Imiquimod-Induced Psoriasiform Dermatitis through Inhibition of IL-17A Expression by Innate γδ-Low T Cells. J Immunol. 2015;195:421-5 pubmed publisher
  • flow cytometry; mouse
Yuan X, Dee M, Altman N, Malek T. IL-2Rβ-dependent signaling and CD103 functionally cooperate to maintain tolerance in the gut mucosa. J Immunol. 2015;194:1334-46 pubmed publisher
  • flow cytometry; mouse; fig 1
Pai M, Liu J, Hou Y, Yeh C. Soybean and Fish Oil Mixture With Different ω-6/ω-3 Polyunsaturated Fatty Acid Ratios Modulates Dextran Sulfate Sodium-Induced Changes in Small Intestinal Intraepithelial γδT-Lymphocyte Expression in Mice. JPEN J Parenter Enteral Nutr. 2016;40:383-91 pubmed publisher
  • flow cytometry; mouse
Mathews J, Williams A, Brand J, Wurmbrand A, Chen L, Ninin F, et al. γδ T cells are required for pulmonary IL-17A expression after ozone exposure in mice: role of TNFα. PLoS ONE. 2014;9:e97707 pubmed publisher
  • immunocytochemistry; mouse
Strid J, Sobolev O, Zafirova B, Polic B, Hayday A. The intraepithelial T cell response to NKG2D-ligands links lymphoid stress surveillance to atopy. Science. 2011;334:1293-7 pubmed publisher
Moore A, Vivanco Gonzalez N, Plummer K, Mitchel O, Kaur H, Rivera M, et al. Gestationally dependent immune organization at the maternal-fetal interface. Cell Rep. 2022;41:111651 pubmed publisher
Wei W, Li J, Shen X, Lyu J, Yan C, Tang B, et al. Oral Microbiota from Periodontitis Promote Oral Squamous Cell Carcinoma Development via γδ T Cell Activation. mSystems. 2022;7:e0046922 pubmed publisher
Goodall K, Nguyen A, Andrews D, Sullivan L. Ribosylation of the CD8αβ heterodimer permits binding of the nonclassical major histocompatibility molecule, H2-Q10. J Biol Chem. 2021;297:101141 pubmed publisher
Cardoso F, Klein Wolterink R, Godinho Silva C, Domingues R, Ribeiro H, da Silva J, et al. Neuro-mesenchymal units control ILC2 and obesity via a brain-adipose circuit. Nature. 2021;597:410-414 pubmed publisher
Ma Z, Cheng X, Yue T, Shangguan X, Xin Z, Zhang W, et al. Immune infiltration phenotypes of prostate adenocarcinoma and their clinical implications. Cancer Med. 2021;10:5358-5374 pubmed publisher
Park S, Matte Martone C, Gonzalez D, Lathrop E, May D, Pineda C, et al. Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis. Nat Cell Biol. 2021;23:476-484 pubmed publisher
Tao H, Li L, Liao N, Schluns K, Luckhart S, Sleasman J, et al. Thymic Epithelial Cell-Derived IL-15 and IL-15 Receptor α Chain Foster Local Environment for Type 1 Innate Like T Cell Development. Front Immunol. 2021;12:623280 pubmed publisher
Lopes N, McIntyre C, Martin S, Raverdeau M, Sumaria N, Kohlgruber A, et al. Distinct metabolic programs established in the thymus control effector functions of γδ T cell subsets in tumor microenvironments. Nat Immunol. 2021;22:179-192 pubmed publisher
Nobs S, Pohlmeier L, Li F, Kayhan M, Becher B, Kopf M. GM-CSF instigates a dendritic cell-T-cell inflammatory circuit that drives chronic asthma development. J Allergy Clin Immunol. 2021;147:2118-2133.e3 pubmed publisher
Castro Dopico T, FLEMING A, Dennison T, Ferdinand J, Harcourt K, Stewart B, et al. GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation. Cell Rep. 2020;32:107857 pubmed publisher
Dasgupta D, Nakao Y, Mauer A, Thompson J, Sehrawat T, Liao C, et al. IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis. Gastroenterology. 2020;159:1487-1503.e17 pubmed publisher
Díaz N, Lang R, Van Gelder R, Buhr E. Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. Invest Ophthalmol Vis Sci. 2020;61:37 pubmed publisher
Zhang M, Slaby E, Stephanie G, Yu C, Watts D, Liu H, et al. Lipid-Mediated Insertion of Toll-Like Receptor (TLR) Ligands for Facile Immune Cell Engineering. Front Immunol. 2020;11:560 pubmed publisher
Bittner Eddy P, Fischer L, Costalonga M. Transient Expression of IL-17A in Foxp3 Fate-Tracked Cells in Porphyromonas gingivalis-Mediated Oral Dysbiosis. Front Immunol. 2020;11:677 pubmed publisher
Álvarez Salamero C, Castillo González R, Pastor Fernández G, Mariblanca I, Pino J, Cibrian D, et al. IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biol. 2020;18:e3000646 pubmed publisher
Dienz O, Devault V, Musial S, Mistri S, Mei L, Baraev A, et al. Critical Role for SLAM/SAP Signaling in the Thymic Developmental Programming of IL-17- and IFN-γ-Producing γδ T Cells. J Immunol. 2020;204:1521-1534 pubmed publisher
Suhail A, Rizvi Z, Mujagond P, Ali S, Gaur P, Singh M, et al. DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells. Cell Rep. 2019;29:3522-3538.e7 pubmed publisher
Yoshizaki T, Itoh S, Yamaguchi S, Numata T, Nambu A, Kimura N, et al. IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice. Sci Rep. 2019;9:17067 pubmed publisher
Castro Dopico T, Dennison T, Ferdinand J, Mathews R, FLEMING A, Clift D, et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis. Immunity. 2019;50:1099-1114.e10 pubmed publisher
Shapiro M, Lehrke M, Chung J, Romero Arocha S, Shapiro V. NKAP Must Associate with HDAC3 to Regulate Hematopoietic Stem Cell Maintenance and Survival. J Immunol. 2019;: pubmed publisher
Yoon B, Kim M, Kim M, Kim H, Kim J, Kim J, et al. Dynamic Transcriptome, DNA Methylome, and DNA Hydroxymethylome Networks During T-Cell Lineage Commitment. Mol Cells. 2018;41:953-963 pubmed publisher
Biljes D, Hammerschmidt Kamper C, Merches K, Esser C. The aryl hydrocarbon receptor in T cells contributes to sustaining oral tolerance against ovalbumin in a mouse model. EXCLI J. 2017;16:291-301 pubmed publisher
Quenum Zangbede F, Chauhan A, Sharma J, Mishra B. Galectin-3 in M2 Macrophages Plays a Protective Role in Resolution of Neuropathology in Brain Parasitic Infection by Regulating Neutrophil Turnover. J Neurosci. 2018;38:6737-6750 pubmed publisher
Mensurado S, Rei M, Lança T, Ioannou M, Gonçalves Sousa N, Kubo H, et al. Tumor-associated neutrophils suppress pro-tumoral IL-17+ γδ T cells through induction of oxidative stress. PLoS Biol. 2018;16:e2004990 pubmed publisher
Roussey J, Viglianti S, Teitz Tennenbaum S, Olszewski M, Osterholzer J. Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. J Immunol. 2017;199:3535-3546 pubmed publisher
Waickman A, Ligons D, Hwang S, Park J, Lazarevic V, Sato N, et al. CD4 effector T cell differentiation is controlled by IL-15 that is expressed and presented in trans. Cytokine. 2017;99:266-274 pubmed publisher
Wei S, Levine J, Cogdill A, Zhao Y, Anang N, Andrews M, et al. Distinct Cellular Mechanisms Underlie Anti-CTLA-4 and Anti-PD-1 Checkpoint Blockade. Cell. 2017;170:1120-1133.e17 pubmed publisher
Fritz Y, Klenotic P, Swindell W, Yin Z, Groft S, Zhang L, et al. Induction of Alternative Proinflammatory Cytokines Accounts for Sustained Psoriasiform Skin Inflammation in IL-17C+IL-6KO Mice. J Invest Dermatol. 2017;137:696-705 pubmed publisher
Van Belle A, de Heusch M, Lemaire M, Hendrickx E, Warnier G, Dunussi Joannopoulos K, et al. IL-22 is required for imiquimod-induced psoriasiform skin inflammation in mice. J Immunol. 2012;188:462-9 pubmed publisher
Stadanlick J, Zhang Z, Lee S, Hemann M, Biery M, Carleton M, et al. Developmental arrest of T cells in Rpl22-deficient mice is dependent upon multiple p53 effectors. J Immunol. 2011;187:664-75 pubmed publisher
Breart B, Ramos Perez W, Mendoza A, Salous A, Gobert M, Huang Y, et al. Lipid phosphate phosphatase 3 enables efficient thymic egress. J Exp Med. 2011;208:1267-78 pubmed publisher
Kasten K, Prakash P, Unsinger J, Goetzman H, England L, Cave C, et al. Interleukin-7 (IL-7) treatment accelerates neutrophil recruitment through gamma delta T-cell IL-17 production in a murine model of sepsis. Infect Immun. 2010;78:4714-22 pubmed publisher
product information
Antigen :
TCR gamma/delta
Apps. Abbrev. :
FC, IHC-F, IP
Cat # :
118101
Clone :
GL3
Item :
Purified anti-mouse TCR γ/δ
Isotype :
Armenian Hamster IgG
Other Names :
T cell receptor γ/δ
Size :
100 μg
Price (USD) :
118 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PURE
Immunogen :
C57BL/6J intraepithelial lymphocytes
Application Notes :
The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemistry of acetone-fixed frozen sections^2,6, and in vivo depletion of γ/δ T cells^3-5.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA