Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex | BioLegend 117612 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

catalog :

117612

quantity :

500 μg

price :

495 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MTS510

reactivity :

mouse

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry

citations: 8

Reference
Oldenburg R, Mayau V, Prandi J, Arbués A, Astarie Dequeker C, Guilhot C, et al. Mycobacterial Phenolic Glycolipids Selectively Disable TRIF-Dependent TLR4 Signaling in Macrophages. Front Immunol. 2018;9:2 pubmed publisher
Ode Y, Aziz M, Wang P. CIRP increases ICAM-1+ phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis. J Leukoc Biol. 2018;103:693-707 pubmed publisher
Okamoto N, Mizote K, Honda H, Saeki A, Watanabe Y, Yamaguchi Miyamoto T, et al. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex. J Biol Chem. 2017;292:15378-15394 pubmed publisher
Westphal A, Cheng W, Yu J, Grassl G, Krautkrämer M, Holst O, et al. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses. J Exp Med. 2017;214:227-244 pubmed publisher
Tanigawa T, Morikawa A, Hayashi K, Dan K, Tsuchihashi N, Goto F, et al. Auditory cells produce nitric oxide in response to bacterial lipopolysaccharide. Innate Immun. 2013;19:115-20 pubmed publisher
Laplante P, Amireault P, Subang R, Dieudé M, Levine J, Rauch J. Interaction of ?2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages. J Biol Chem. 2011;286:42494-503 pubmed publisher
Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, et al. Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. Blood. 2011;117:2211-22 pubmed publisher
Nalbandian G, Paharkova Vatchkova V, Mao A, Nale S, Kovats S. The selective estrogen receptor modulators, tamoxifen and raloxifene, impair dendritic cell differentiation and activation. J Immunol. 2005;175:2666-75 pubmed

product information

Antigen :

TLR4 MD2 Complex

Apps. Abbrev. :

Flow Cytometry, IHC, Intracellular Staining for Flow Cytometry, Immunofluorescence Microscopy, IP, Block

Cat # :

117612

Clone :

MTS510

Item :

Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

Isotype :

Rat IgG2a, κ

Other Names :

Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2

Size :

500 μg

Price (USD) :

495 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

Ba/F3 cell line transfected with mouse TLR4 /MD2 complex

Application Notes :

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other. 8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation. 8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).