LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex | BioLegend 117607 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

catalog :

117607

quantity :

50 ug

price :

150 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MTS510

reactivity :

mouse

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 3	Kimura T, Endo S, Inui M, Saitoh S, Miyake K, Takai T. Endoplasmic Protein Nogo-B (RTN4-B) Interacts with GRAMD4 and Regulates TLR9-Mediated Innate Immune Responses. J Immunol. 2015;194:5426-36 pubmed publisher
	Oldenburg R, Mayau V, Prandi J, Arbués A, Astarie Dequeker C, Guilhot C, et al. Mycobacterial Phenolic Glycolipids Selectively Disable TRIF-Dependent TLR4 Signaling in Macrophages. Front Immunol. 2018;9:2 pubmed publisher
	Ode Y, Aziz M, Wang P. CIRP increases ICAM-1+ phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis. J Leukoc Biol. 2018;103:693-707 pubmed publisher
	Shen Y, Guo D, Weng L, Wang S, Ma Z, Yang Y, et al. Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway. Oncoimmunology. 2017;6:e1362527 pubmed publisher
	Okamoto N, Mizote K, Honda H, Saeki A, Watanabe Y, Yamaguchi Miyamoto T, et al. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex. J Biol Chem. 2017;292:15378-15394 pubmed publisher
	Westphal A, Cheng W, Yu J, Grassl G, Krautkrämer M, Holst O, et al. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses. J Exp Med. 2017;214:227-244 pubmed publisher
	Karki R, Igwe O. Toll-like receptor 4-mediated nuclear factor kappa B activation is essential for sensing exogenous oxidants to propagate and maintain oxidative/nitrosative cellular stress. PLoS ONE. 2013;8:e73840 pubmed publisher
	Ma Y, Zhang X, Bao H, Mi S, Cai W, Yan H, et al. Toll-like receptor (TLR) 2 and TLR4 differentially regulate doxorubicin induced cardiomyopathy in mice. PLoS ONE. 2012;7:e40763 pubmed publisher
	Tanigawa T, Morikawa A, Hayashi K, Dan K, Tsuchihashi N, Goto F, et al. Auditory cells produce nitric oxide in response to bacterial lipopolysaccharide. Innate Immun. 2013;19:115-20 pubmed publisher
	Laplante P, Amireault P, Subang R, Dieudé M, Levine J, Rauch J. Interaction of ?2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages. J Biol Chem. 2011;286:42494-503 pubmed publisher
	Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, et al. Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. Blood. 2011;117:2211-22 pubmed publisher
	Nalbandian G, Paharkova Vatchkova V, Mao A, Nale S, Kovats S. The selective estrogen receptor modulators, tamoxifen and raloxifene, impair dendritic cell differentiation and activation. J Immunol. 2005;175:2666-75 pubmed

product information

Antigen :

TLR4 MD2 Complex

Apps. Abbrev. :

Flow Cytometry, IHC, Intracellular Staining for Flow Cytometry, Immunofluorescence Microscopy, IP, Block

Cat # :

117607

Clone :

MTS510

Item :

LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

Isotype :

Rat IgG2a, kappa

Other Names :

Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2

Size :

50 ug

Price (USD) :

150 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

Ba/F3 cell line transfected with mouse TLR4 /MD2 complex

Application Notes :

. Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other. 8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation. 8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).