Purified anti-mouse TLR4 (CD284)/MD2 Complex | BioLegend 117602 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse TLR4 (CD284)/MD2 Complex

catalog :

117602

quantity :

200 μg

price :

235 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MTS510

reactivity :

mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

BioLegend product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1a immunoprecipitation; mouse; loading ...; fig 8d	Wu Y, Ren D, Chen G. Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis. J Immunol. 2016;197:3336-3347 pubmed
flow cytometry; mouse; fig 3	Kimura T, Endo S, Inui M, Saitoh S, Miyake K, Takai T. Endoplasmic Protein Nogo-B (RTN4-B) Interacts with GRAMD4 and Regulates TLR9-Mediated Innate Immune Responses. J Immunol. 2015;194:5426-36 pubmed publisher
flow cytometry; mouse; 1:200	Kanayama M, Inoue M, Danzaki K, Hammer G, He Y, Shinohara M. Autophagy enhances NFÎºB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nat Commun. 2015;6:5779 pubmed publisher
	Takimoto Y, Chu P, Nakamoto N, Hagihara Y, Mikami Y, Miyamoto K, et al. Myeloid TLR4 signaling promotes post-injury withdrawal resolution of murine liver fibrosis. iScience. 2023;26:106220 pubmed publisher
	Shimizu J, Murao A, Nofi C, Wang P, Aziz M. Extracellular CIRP Promotes GPX4-Mediated Ferroptosis in Sepsis. Front Immunol. 2022;13:903859 pubmed publisher
	Ricci Azevedo R, Mendon xe7 a Natividade F, Santana A, Alcoforado Diniz J, Roque Barreira M. Microneme Proteins 1 and 4 From Toxoplasma gondii Induce IL-10 Production by Macrophages Through TLR4 Endocytosis. Front Immunol. 2021;12:655371 pubmed publisher
	Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
	Suzuki H, Yamazaki T, Ohshio K, Sugamata M, Yoshikawa M, Kanauchi O, et al. A Specific Strain of Lactic Acid Bacteria, Lactobacillus paracasei, Inhibits Inflammasome Activation In Vitro and Prevents Inflammation-Related Disorders. J Immunol. 2020;205:811-821 pubmed publisher
	Poznanski S, Nham T, Chew M, Lee A, Hammill J, Fan I, et al. Expanded CD56superbrightCD16+ NK Cells from Ovarian Cancer Patients Are Cytotoxic against Autologous Tumor in a Patient-Derived Xenograft Murine Model. Cancer Immunol Res. 2018;6:1174-1185 pubmed publisher
	Oldenburg R, Mayau V, Prandi J, Arbués A, Astarie Dequeker C, Guilhot C, et al. Mycobacterial Phenolic Glycolipids Selectively Disable TRIF-Dependent TLR4 Signaling in Macrophages. Front Immunol. 2018;9:2 pubmed publisher
	Ode Y, Aziz M, Wang P. CIRP increases ICAM-1+ phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis. J Leukoc Biol. 2018;103:693-707 pubmed publisher
	Okamoto N, Mizote K, Honda H, Saeki A, Watanabe Y, Yamaguchi Miyamoto T, et al. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex. J Biol Chem. 2017;292:15378-15394 pubmed publisher
	Westphal A, Cheng W, Yu J, Grassl G, Krautkrämer M, Holst O, et al. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses. J Exp Med. 2017;214:227-244 pubmed publisher
	Tanigawa T, Morikawa A, Hayashi K, Dan K, Tsuchihashi N, Goto F, et al. Auditory cells produce nitric oxide in response to bacterial lipopolysaccharide. Innate Immun. 2013;19:115-20 pubmed publisher
	Long C, Hosseinkhani M, Wang Y, Sriramarao P, Walcheck B. ADAM17 activation in circulating neutrophils following bacterial challenge impairs their recruitment. J Leukoc Biol. 2012;92:667-72 pubmed publisher
	Laplante P, Amireault P, Subang R, Dieudé M, Levine J, Rauch J. Interaction of ?2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages. J Biol Chem. 2011;286:42494-503 pubmed publisher
	Saxena A, Rauch U, Berg K, Andersson L, Hollender L, Carlsson A, et al. The vascular repair process after injury of the carotid artery is regulated by IL-1RI and MyD88 signalling. Cardiovasc Res. 2011;91:350-7 pubmed publisher
	Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, et al. Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. Blood. 2011;117:2211-22 pubmed publisher
	Sheng K, Pietersz G, Tang C, Ramsland P, Apostolopoulos V. Reactive oxygen species level defines two functionally distinctive stages of inflammatory dendritic cell development from mouse bone marrow. J Immunol. 2010;184:2863-72 pubmed publisher
	Yang H, Cui B, Liu H, Mi S, Yan J, Yan H, et al. Blocking TLR2 activity attenuates pulmonary metastases of tumor. PLoS ONE. 2009;4:e6520 pubmed publisher
	Iwata Y, Yoshizaki A, Komura K, Shimizu K, Ogawa F, Hara T, et al. CD19, a response regulator of B lymphocytes, regulates wound healing through hyaluronan-induced TLR4 signaling. Am J Pathol. 2009;175:649-60 pubmed publisher
	Suzuki M, Tachibana I, Takeda Y, He P, Minami S, Iwasaki T, et al. Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation. J Immunol. 2009;182:6485-93 pubmed publisher
	Sheng K, Kalkanidis M, Pouniotis D, Wright M, Pietersz G, Apostolopoulos V. The adjuvanticity of a mannosylated antigen reveals TLR4 functionality essential for subset specialization and functional maturation of mouse dendritic cells. J Immunol. 2008;181:2455-64 pubmed
	Bhattacharyya S, Borthakur A, Pant N, Dudeja P, Tobacman J. Bcl10 mediates LPS-induced activation of NF-kappaB and IL-8 in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007;293:G429-37 pubmed
	Mao A, Paharkova Vatchkova V, Hardy J, Miller M, Kovats S. Estrogen selectively promotes the differentiation of dendritic cells with characteristics of Langerhans cells. J Immunol. 2005;175:5146-51 pubmed
	Nalbandian G, Paharkova Vatchkova V, Mao A, Nale S, Kovats S. The selective estrogen receptor modulators, tamoxifen and raloxifene, impair dendritic cell differentiation and activation. J Immunol. 2005;175:2666-75 pubmed

product information

Antigen :

TLR4 MD2 Complex

Apps. Abbrev. :

FC, IHC-P, WB, ICFC, IP

Cat # :

117602

Clone :

MTS510

Item :

Purified anti-mouse TLR4 (CD284)/MD2 Complex

Isotype :

Rat IgG2a, κ

Other Names :

Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2

Size :

200 μg

Price (USD) :

235 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

Ba/F3 cell line transfected with mouse TLR4 /MD2 complex

Application Notes :

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don't cross block each other.^8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.^8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage