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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse H-2Kb
catalog :
116501
quantity :
50 μg
price :
77 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AF6-88.5
reactivity :
mouse
application :
immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s6b
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; mouse; fig 3a, 3b, 4b
Aiken T, Erbe A, Zebertavage L, Komjathy D, Feils A, Rodriguez M, et al. Mechanism of effective combination radio-immunotherapy against 9464D-GD2, an immunologically cold murine neuroblastoma. J Immunother Cancer. 2022;10: pubmed publisher
  • blocking or activating experiments; mouse; fig 2d
  • flow cytometry; mouse; loading ...; fig 2c
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; fig 4a
Sivaram N, McLaughlin P, Han H, Petrenko O, Jiang Y, Ballou L, et al. Tumor-intrinsic PIK3CA represses tumor immunogenecity in a model of pancreatic cancer. J Clin Invest. 2019;130: pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Bern M, Parikh B, Yang L, Beckman D, Poursine Laurent J, Yokoyama W. Inducible down-regulation of MHC class I results in natural killer cell tolerance. J Exp Med. 2019;216:99-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Magallanes Puebla A, Espinosa Cueto P, López Marín L, Mancilla R. Mycobacterial glycolipid Di-O-acyl trehalose promotes a tolerogenic profile in dendritic cells. PLoS ONE. 2018;13:e0207202 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8a
Theisen D, Davidson J, Briseño C, Gargaro M, Lauron E, Wang Q, et al. WDFY4 is required for cross-presentation in response to viral and tumor antigens. Science. 2018;362:694-699 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Wilson K, Liu H, Healey G, Vuong V, Ishido S, Herold M, et al. MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. PLoS ONE. 2018;13:e0200540 pubmed publisher
  • flow cytometry; mouse; fig 6a
Swanson P, Hart G, Russo M, Nayak D, Yazew T, Pena M, et al. CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature. PLoS Pathog. 2016;12:e1006022 pubmed publisher
  • flow cytometry; mouse; fig 2
Huang M, Zhang W, Guo J, Wei X, Phiwpan K, Zhang J, et al. Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation. Sci Rep. 2016;6:33612 pubmed publisher
  • flow cytometry; mouse
Oliveira C, Querido B, Sluijter M, de Groot A, van der Zee R, Rabelink M, et al. New role of signal peptide peptidase to liberate C-terminal peptides for MHC class I presentation. J Immunol. 2013;191:4020-8 pubmed publisher
Tello Lafoz M, Srpan K, Sanchez E, Hu J, Remšík J, Romin Y, et al. Cytotoxic lymphocytes target characteristic biophysical vulnerabilities in cancer. Immunity. 2021;54:1037-1054.e7 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Sutiwisesak R, Hicks N, Boyce S, Murphy K, Papavinasasundaram K, Carpenter S, et al. A natural polymorphism of Mycobacterium tuberculosis in the esxH gene disrupts immunodomination by the TB10.4-specific CD8 T cell response. PLoS Pathog. 2020;16:e1009000 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Ferrari de Andrade L, Kumar S, Luoma A, Ito Y, Alves da Silva P, Pan D, et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res. 2020;8:769-780 pubmed publisher
Williams J, Li S, Higgs E, Cabanov A, Wang X, Huang H, et al. Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells. Nat Commun. 2020;11:602 pubmed publisher
Zhou L, Mudianto T, Ma X, Riley R, Uppaluri R. Targeting EZH2 Enhances Antigen Presentation, Antitumor Immunity, and Circumvents Anti-PD-1 Resistance in Head and Neck Cancer. Clin Cancer Res. 2019;: pubmed publisher
Shao L, Hou W, Scharping N, Vendetti F, Srivastava R, Roy C, et al. IRF1 Inhibits Antitumor Immunity through the Upregulation of PD-L1 in the Tumor Cell. Cancer Immunol Res. 2019;7:1258-1266 pubmed publisher
Hafstrand I, Sayitoglu E, Apavaloaei A, Josey B, Sun R, Han X, et al. Successive crystal structure snapshots suggest the basis for MHC class I peptide loading and editing by tapasin. Proc Natl Acad Sci U S A. 2019;116:5055-5060 pubmed publisher
Lee Y, Ju J, Shon W, Oh S, Min C, Kang M, et al. Skewed Dendritic Cell Differentiation of MyD88-Deficient Donor Bone Marrow Cells, Instead of Massive Expansion as Myeloid-Derived Suppressor Cells, Aggravates GVHD. Immune Netw. 2018;18:e44 pubmed publisher
Gejman R, Chang A, Jones H, DiKun K, Hakimi A, Schietinger A, et al. Rejection of immunogenic tumor clones is limited by clonal fraction. elife. 2018;7: pubmed publisher
Thyagarajan H, Lancaster J, Lira S, Ehrlich L. CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction. PLoS ONE. 2018;13:e0200765 pubmed publisher
Stefanski H, Xing Y, Taylor P, Maio S, Henao Meija J, Williams A, et al. Despite high levels of expression in thymic epithelial cells, miR-181a1 and miR-181b1 are not required for thymic development. PLoS ONE. 2018;13:e0198871 pubmed publisher
Chaganty B, Qiu S, Gest A, Lu Y, Ivan C, Calin G, et al. Trastuzumab upregulates PD-L1 as a potential mechanism of trastuzumab resistance through engagement of immune effector cells and stimulation of IFN? secretion. Cancer Lett. 2018;430:47-56 pubmed publisher
Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell. 2018;173:634-648.e12 pubmed publisher
Robert S, Albring J, Frebel K, Opitz C, Urh J, Wolf C, et al. Alloantigen expression on malignant cells and healthy host tissue influences graft-versus-tumor reactions after allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 2018;53:807-819 pubmed publisher
Toubai T, Rossi C, Oravecz Wilson K, Liu C, Zajac C, Wu S, et al. IAPs protect host target tissues from graft-versus-host disease in mice. Blood Adv. 2017;1:1517-1532 pubmed publisher
Cowan J, Baik S, McCarthy N, Parnell S, White A, Jenkinson W, et al. Aire controls the recirculation of murine Foxp3+ regulatory T-cells back to the thymus. Eur J Immunol. 2018;48:844-854 pubmed publisher
Jain R, Sheridan J, Policheni A, Heinlein M, Gandolfo L, Dewson G, et al. A critical epithelial survival axis regulated by MCL-1 maintains thymic function in mice. Blood. 2017;130:2504-2515 pubmed publisher
Lupfer C, Stokes K, Kuriakose T, Kanneganti T. Deficiency of the NOD-Like Receptor NLRC5 Results in Decreased CD8+ T Cell Function and Impaired Viral Clearance. J Virol. 2017;91: pubmed publisher
Davis R, Moore E, Clavijo P, Friedman J, Cash H, Chen Z, et al. Anti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3K?/?. Cancer Res. 2017;77:2607-2619 pubmed publisher
E S, Seth A, Vogel P, Sommers M, Ong T, Pillai A. Bidirectional immune tolerance in nonmyeloablative MHC-mismatched BMT for murine β-thalassemia. Blood. 2017;129:3017-3030 pubmed publisher
Chamoto K, Chowdhury P, Kumar A, Sonomura K, Matsuda F, Fagarasan S, et al. Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E761-E770 pubmed publisher
Desvignes L, Wolf A, Ernst J. Dynamic roles of type I and type II IFNs in early infection with Mycobacterium tuberculosis. J Immunol. 2012;188:6205-15 pubmed publisher
Zhou K, Zhu H, Huang L, Guo Y, Yan Y. Induction of anti-tumor immunity by dendritic cells pulsed with an endoplasmic reticulum retrieval signal modifies heparanase epitope in mice. Cytotherapy. 2010;12:735-42 pubmed publisher
Shao H, Peng Y, Liao T, Wang M, Song M, Kaplan H, et al. A shared epitope of the interphotoreceptor retinoid-binding protein recognized by the CD4+ and CD8+ autoreactive T cells. J Immunol. 2005;175:1851-7 pubmed
product information
Antigen :
H-2Kb
Apps. Abbrev. :
FC, IHC-F, IP
Cat # :
116501
Clone :
AF6-88.5
Item :
Purified anti-mouse H-2Kb
Isotype :
Mouse (BALB/c) IgG2a, κ
Other Names :
MHC class I
Size :
50 μg
Price (USD) :
77 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
C57BL mouse splenocytes
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^2 and immunohistochemical staining of acetone-fixed frozen sections^3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA