product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD54
catalog :
116113
quantity :
25 μg
price :
101 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
YN1/1.7.4
reactivity :
mouse
application :
western blot, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; fig 3e
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • flow cytometry; mouse; fig 2d
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • blocking or activating experiments; mouse; fig 2c
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • western blot; mouse; loading ...; fig 7a
Mentrup T, Theodorou K, Cabrera Cabrera F, Helbig A, Happ K, Gijbels M, et al. Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
Neupane A, Willson M, Chojnacki A, Vargas E Silva Castanheira F, Morehouse C, Carestia A, et al. Patrolling Alveolar Macrophages Conceal Bacteria from the Immune System to Maintain Homeostasis. Cell. 2020;183:110-125.e11 pubmed publisher
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed publisher
Lauterbach M, Hanke J, Serefidou M, Mangan M, Kolbe C, Hess T, et al. Toll-like Receptor Signaling Rewires Macrophage Metabolism and Promotes Histone Acetylation via ATP-Citrate Lyase. Immunity. 2019;51:997-1011.e7 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
Ibi M, Horie S, Kyakumoto S, Chosa N, Yoshida M, Kamo M, et al. Cell-cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint. Biosci Rep. 2018;38: pubmed publisher
Deniset J, Surewaard B, Lee W, Kubes P. Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of S. pneumoniae. J Exp Med. 2017;214:1333-1350 pubmed publisher
Parsa R, Lund H, Georgoudaki A, Zhang X, Ortlieb Guerreiro Cacais A, Grommisch D, et al. BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis. J Exp Med. 2016;213:1537-53 pubmed publisher
Malinova D, Fritzsche M, Nowosad C, Armer H, Munro P, Blundell M, et al. WASp-dependent actin cytoskeleton stability at the dendritic cell immunological synapse is required for extensive, functional T cell contacts. J Leukoc Biol. 2016;99:699-710 pubmed publisher
Greineder C, Chacko A, Zaytsev S, Zern B, Carnemolla R, Hood E, et al. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. PLoS ONE. 2013;8:e80110 pubmed publisher
El Assaad F, Wheway J, Mitchell A, Lou J, Hunt N, Combes V, et al. Cytoadherence of Plasmodium berghei-infected red blood cells to murine brain and lung microvascular endothelial cells in vitro. Infect Immun. 2013;81:3984-91 pubmed publisher
Darbousset R, Thomas G, Mezouar S, Frere C, Bonier R, Mackman N, et al. Tissue factor-positive neutrophils bind to injured endothelial wall and initiate thrombus formation. Blood. 2012;120:2133-43 pubmed publisher
Seidler D, Mohamed N, Bocian C, Stadtmann A, Hermann S, Schafers K, et al. The role for decorin in delayed-type hypersensitivity. J Immunol. 2011;187:6108-19 pubmed publisher
Chacko A, Hood E, Zern B, Muzykantov V. Targeted Nanocarriers for Imaging and Therapy of Vascular Inflammation. Curr Opin Colloid Interface Sci. 2011;16:215-227 pubmed
Sadrai Z, Hajrasouliha A, Chauhan S, Saban D, Dastjerdi M, Dana R. Effect of topical azithromycin on corneal innate immune responses. Invest Ophthalmol Vis Sci. 2011;52:2525-31 pubmed publisher
Zhou P, Qian L, Gallo E, Deeb R, Anrather J, Gross S, et al. The scavenger receptor CD36 contributes to the neurotoxicity of bone marrow-derived monocytes through peroxynitrite production. Neurobiol Dis. 2011;42:292-9 pubmed publisher
Palazon A, Teijeira A, Martinez Forero I, Hervas Stubbs S, Roncal C, Penuelas I, et al. Agonist anti-CD137 mAb act on tumor endothelial cells to enhance recruitment of activated T lymphocytes. Cancer Res. 2011;71:801-11 pubmed publisher
Sumagin R, Sarelius I. Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage. J Immunol. 2010;184:5242-52 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
product information
Antigen :
CD54
Apps. Abbrev. :
FC
Cat # :
116113
Clone :
YN1/1.7.4
Item :
Alexa Fluor® 647 anti-mouse CD54
Isotype :
Rat IgG2b, κ
Other Names :
ICAM-1, Ly-47
Size :
25 μg
Price (USD) :
101 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Mouse NS-1 cells
Application Notes :
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions^1,2,4, immunohistochemical staining^3 of acetone-fixed frozen sections, immunoprecipitation^4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA