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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse CD54
catalog :
116102
quantity :
500 μg
price :
115 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
YN1/1.7.4
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 7a
Mentrup T, Theodorou K, Cabrera Cabrera F, Helbig A, Happ K, Gijbels M, et al. Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis. J Exp Med. 2019;: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4a
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 1c
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3a
Qiu C, Wang Y, Zhao H, Qin L, Shi Y, Zhu X, et al. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nat Commun. 2017;8:15426 pubmed publisher
  • flow cytometry; mouse; fig 7
Ushiki T, Huntington N, Glaser S, Kiu H, Georgiou A, Zhang J, et al. Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells. PLoS ONE. 2016;11:e0162111 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5k
Barin J, Talor M, Schaub J, Diny N, Hou X, Hoyer M, et al. Collaborative Interferon-? and Interleukin-17 Signaling Protects the Oral Mucosa from Staphylococcus aureus. Am J Pathol. 2016;186:2337-52 pubmed publisher
  • flow cytometry; mouse; fig 4e
Parsa R, Lund H, Georgoudaki A, Zhang X, Ortlieb Guerreiro Cacais A, Grommisch D, et al. BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis. J Exp Med. 2016;213:1537-53 pubmed publisher
  • flow cytometry; mouse; fig 2
Gawade S, Mayer C, Hafen K, Barthlott T, Krenger W, Szinnai G. Cell Growth Dynamics in Embryonic and Adult Mouse Thyroid Revealed by a Novel Approach to Detect Thyroid Gland Subpopulations. Thyroid. 2016;26:591-9 pubmed publisher
  • flow cytometry; mouse; loading ...
Malinova D, Fritzsche M, Nowosad C, Armer H, Munro P, Blundell M, et al. WASp-dependent actin cytoskeleton stability at the dendritic cell immunological synapse is required for extensive, functional T cell contacts. J Leukoc Biol. 2016;99:699-710 pubmed publisher
  • flow cytometry; mouse; fig 1
Fontinha D, Lopes F, Marques S, Alenquer M, Simas J. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS ONE. 2015;10:e0142540 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 1h
Yoshida Y, Shimizu I, Katsuumi G, Jiao S, Suda M, Hayashi Y, et al. p53-Induced inflammation exacerbates cardiac dysfunction during pressure overload. J Mol Cell Cardiol. 2015;85:183-98 pubmed publisher
  • flow cytometry; mouse; 1:50; fig 2a
Lujan E, Zunder E, Ng Y, Goronzy I, Nolan G, Wernig M. Early reprogramming regulators identified by prospective isolation and mass cytometry. Nature. 2015;521:352-6 pubmed publisher
  • immunocytochemistry; mouse
Nacer A, Movila A, Sohet F, Girgis N, Gundra U, Loke P, et al. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014;10:e1004528 pubmed publisher
Douglas G, Mehta V, Zen A, Akoumianakis I, Goel A, Rashbrook V, et al. A key role for the novel coronary artery disease gene JCAD in atherosclerosis via shear stress mechanotransduction. Cardiovasc Res. 2019;: pubmed publisher
Chen P, Qin L, Li G, Wang Z, Dahlman J, Malagon Lopez J, et al. Endothelial TGF-β signalling drives vascular inflammation and atherosclerosis. Nat Metab. 2019;1:912-926 pubmed publisher
Yun S, Hu R, Schwaemmle M, Scherer A, Zhuang Z, Koleske A, et al. Integrin α5β1 regulates PP2A complex assembly through PDE4D in atherosclerosis. J Clin Invest. 2019;129:4863-4874 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
Echeverria G, Powell E, Seth S, Ge Z, Carugo A, BRISTOW C, et al. High-resolution clonal mapping of multi-organ metastasis in triple negative breast cancer. Nat Commun. 2018;9:5079 pubmed publisher
Clark V, Layson A, Charkviani M, Muradashvili N, Lominadze D. Hyperfibrinogenemia-mediated astrocyte activation. Brain Res. 2018;1699:158-165 pubmed publisher
Tong C, Zhang Y, Lü S, Li N, Gong Y, Yang H, et al. Binding of intercellular adhesion molecule 1 to β2-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium. Am J Physiol Cell Physiol. 2018;315:C409-C421 pubmed publisher
Ibi M, Horie S, Kyakumoto S, Chosa N, Yoshida M, Kamo M, et al. Cell-cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint. Biosci Rep. 2018;38: pubmed publisher
Wolf D, Anto Michel N, Blankenbach H, Wiedemann A, Buscher K, Hohmann J, et al. A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense. Nat Commun. 2018;9:525 pubmed publisher
Saitoh S, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, et al. TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells. Nat Commun. 2017;8:1592 pubmed publisher
Deniset J, Surewaard B, Lee W, Kubes P. Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of S. pneumoniae. J Exp Med. 2017;214:1333-1350 pubmed publisher
Yun S, Budatha M, Dahlman J, Coon B, Cameron R, Langer R, et al. Interaction between integrin ?5 and PDE4D regulates endothelial inflammatory signalling. Nat Cell Biol. 2016;18:1043-53 pubmed publisher
Greineder C, Chacko A, Zaytsev S, Zern B, Carnemolla R, Hood E, et al. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. PLoS ONE. 2013;8:e80110 pubmed publisher
El Assaad F, Wheway J, Mitchell A, Lou J, Hunt N, Combes V, et al. Cytoadherence of Plasmodium berghei-infected red blood cells to murine brain and lung microvascular endothelial cells in vitro. Infect Immun. 2013;81:3984-91 pubmed publisher
Seidler D, Mohamed N, Bocian C, Stadtmann A, Hermann S, Schafers K, et al. The role for decorin in delayed-type hypersensitivity. J Immunol. 2011;187:6108-19 pubmed publisher
Chacko A, Hood E, Zern B, Muzykantov V. Targeted Nanocarriers for Imaging and Therapy of Vascular Inflammation. Curr Opin Colloid Interface Sci. 2011;16:215-227 pubmed
Liu J, Weller G, Zern B, Ayyaswamy P, Eckmann D, Muzykantov V, et al. Computational model for nanocarrier binding to endothelium validated using in vivo, in vitro, and atomic force microscopy experiments. Proc Natl Acad Sci U S A. 2010;107:16530-5 pubmed publisher
Sumagin R, Sarelius I. Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage. J Immunol. 2010;184:5242-52 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
Liu Y, Matsui O. Changes of intratumoral microvessels and blood perfusion during establishment of hepatic metastases in mice. Radiology. 2007;243:386-95 pubmed
product information
Antigen :
CD54
Apps. Abbrev. :
Flow Cytometry, IHC-F, IP, Western Blotting
Cat # :
116102
Clone :
YN1/1.7.4
Item :
Purified anti-mouse CD54
Isotype :
Rat IgG2b, κ
Other Names :
ICAM-1, Ly-47
Size :
500 μg
Price (USD) :
115 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
Mouse NS-1 cells
Application Notes :
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions 1,2,4 , immunohistochemical staining 3 of acetone-fixed frozen sections, immunoprecipitation 4 , and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA