product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD150 (SLAM)
catalog :
115916
quantity :
100 μg
price :
265 USD
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
TC15-12F12.2
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse
Vavassori V, Mercuri E, Marcovecchio G, Castiello M, Schiroli G, Albano L, et al. Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome. EMBO Mol Med. 2021;13:e13545 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; loading ...
Ricci B, Tycksen E, Celik H, Belle J, Fontana F, Civitelli R, et al. Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 4a
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7c
Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig ex9j
Tikhonova A, Dolgalev I, Hu H, Sivaraj K, Hoxha E, Cuesta Dominguez A, et al. The bone marrow microenvironment at single-cell resolution. Nature. 2019;569:222-228 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b, 6e
Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ev2g
Rao T, Gupta M, Softic S, Wang L, Jang Y, Thomou T, et al. Attenuation of PKCδ enhances metabolic activity and promotes expansion of blood progenitors. EMBO J. 2018;37: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Umemoto T, Hashimoto M, Matsumura T, Nakamura Ishizu A, Suda T. Ca2+-mitochondria axis drives cell division in hematopoietic stem cells. J Exp Med. 2018;215:2097-2113 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5g
Oda A, Tezuka T, Ueno Y, Hosoda S, Amemiya Y, Notsu C, et al. Niche-induced extramedullary hematopoiesis in the spleen is regulated by the transcription factor Tlx1. Sci Rep. 2018;8:8308 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1e
Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s1a
Chapple R, Hu T, Tseng Y, Liu L, Kitano A, Luu V, et al. ERα promotes murine hematopoietic regeneration through the Ire1α-mediated unfolded protein response. elife. 2018;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Lu Z, Hong C, Kong G, Assumpção A, Ong I, Bresnick E, et al. Polycomb Group Protein YY1 Is an Essential Regulator of Hematopoietic Stem Cell Quiescence. Cell Rep. 2018;22:1545-1559 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Mumau M, Vanderbeck A, Lynch E, Golec S, Emerson S, Punt J. Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol. 2018;200:1078-1087 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1c
Hérault A, Binnewies M, Leong S, Calero Nieto F, Zhang S, Kang Y, et al. Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis. Nature. 2017;544:53-58 pubmed publisher
  • flow cytometry; mouse
Kerenyi M, Shao Z, Hsu Y, Guo G, Luc S, O Brien K, et al. Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. elife. 2013;2:e00633 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
  • flow cytometry; mouse
Yvan Charvet L, Pagler T, Gautier E, Avagyan S, Siry R, Han S, et al. ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation. Science. 2010;328:1689-93 pubmed publisher
Carpenter R, Marbourg J, Brennan F, Mifflin K, Hall J, Jiang R, et al. Spinal cord injury causes chronic bone marrow failure. Nat Commun. 2020;11:3702 pubmed publisher
Yang C, Chen F, Ren P, Lofchy L, Wan C, Shen J, et al. Delivery of a model lipophilic membrane cargo to bone marrow via cell-derived microparticles. J Control Release. 2020;326:324-334 pubmed publisher
Dobie R, Wilson Kanamori J, Henderson B, Smith J, Matchett K, Portman J, et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep. 2019;29:1832-1847.e8 pubmed publisher
Hsu J, Dayaram T, Tovy A, De Braekeleer E, Jeong M, Wang F, et al. PPM1D Mutations Drive Clonal Hematopoiesis in Response to Cytotoxic Chemotherapy. Cell Stem Cell. 2018;23:700-713.e6 pubmed publisher
Boulais P, Mizoguchi T, Zimmerman S, Nakahara F, Vivié J, Mar J, et al. The Majority of CD45- Ter119- CD31- Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors. Immunity. 2018;49:627-639.e6 pubmed publisher
Bellelli R, Borel V, Logan C, Svendsen J, Cox D, Nye E, et al. Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell. 2018;70:707-721.e7 pubmed publisher
Jiang L, Han X, Wang J, Wang C, Sun X, Xie J, et al. SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling. J Exp Med. 2018;215:1337-1347 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, et al. A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018;48:688-701.e7 pubmed publisher
Seike M, Omatsu Y, Watanabe H, Kondoh G, Nagasawa T. Stem cell niche-specific Ebf3 maintains the bone marrow cavity. Genes Dev. 2018;32:359-372 pubmed publisher
Tusi B, Wolock S, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, et al. Population snapshots predict early haematopoietic and erythroid hierarchies. Nature. 2018;555:54-60 pubmed publisher
Zhang H, Li H, Hillmer E, Zhao Y, Chrisikos T, Hu H, et al. Genetic rescue of lineage-balanced blood cell production reveals a crucial role for STAT3 antiinflammatory activity in hematopoiesis. Proc Natl Acad Sci U S A. 2018;115:E2311-E2319 pubmed publisher
McKenney A, Lau A, Somasundara A, Spitzer B, Intlekofer A, Ahn J, et al. JAK2/IDH-mutant-driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. J Clin Invest. 2018;128:789-804 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Cole C, Russler Germain D, Ketkar S, Verdoni A, Smith A, Bangert C, et al. Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. J Clin Invest. 2017;127:3657-3674 pubmed publisher
Umemoto T, Matsuzaki Y, Shiratsuchi Y, Hashimoto M, Yoshimoto T, Nakamura Ishizu A, et al. Integrin αvβ3 enhances the suppressive effect of interferon-γ on hematopoietic stem cells. EMBO J. 2017;36:2390-2403 pubmed publisher
Gullicksrud J, Li F, Xing S, Zeng Z, Peng W, Badovinac V, et al. Differential Requirements for Tcf1 Long Isoforms in CD8+ and CD4+ T Cell Responses to Acute Viral Infection. J Immunol. 2017;199:911-919 pubmed publisher
Lefrançais E, Ortiz Muñoz G, Caudrillier A, Mallavia B, Liu F, Sayah D, et al. The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors. Nature. 2017;544:105-109 pubmed publisher
Kramer A, Kothari A, Wilson W, Celik H, Nikitas J, Mallaney C, et al. Dnmt3a regulates T-cell development and suppresses T-ALL transformation. Leukemia. 2017;31:2479-2490 pubmed publisher
Pleines I, Woods J, Chappaz S, Kew V, Foad N, Ballester Beltrán J, et al. Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia. J Clin Invest. 2017;127:814-829 pubmed publisher
Nish S, Zens K, Kratchmarov R, Lin W, Adams W, Chen Y, et al. CD4+ T cell effector commitment coupled to self-renewal by asymmetric cell divisions. J Exp Med. 2017;214:39-47 pubmed publisher
Snell L, Osokine I, Yamada D, de la Fuente J, Elsaesser H, Brooks D. Overcoming CD4 Th1 Cell Fate Restrictions to Sustain Antiviral CD8 T Cells and Control Persistent Virus Infection. Cell Rep. 2016;16:3286-3296 pubmed publisher
Mizuochi C, Fraser S, Biasch K, Horio Y, Kikushige Y, Tani K, et al. Intra-aortic clusters undergo endothelial to hematopoietic phenotypic transition during early embryogenesis. PLoS ONE. 2012;7:e35763 pubmed publisher
Kunimoto H, Fukuchi Y, Sakurai M, Sadahira K, Ikeda Y, Okamoto S, et al. Tet2 disruption leads to enhanced self-renewal and altered differentiation of fetal liver hematopoietic stem cells. Sci Rep. 2012;2:273 pubmed publisher
Morita Y, Ema H, Nakauchi H. Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment. J Exp Med. 2010;207:1173-82 pubmed publisher
Song J, Kiel M, Wang Z, Wang J, Taichman R, Morrison S, et al. An in vivo model to study and manipulate the hematopoietic stem cell niche. Blood. 2010;115:2592-600 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
Jung Y, Song J, Shiozawa Y, Wang J, Wang Z, Williams B, et al. Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell niche. Stem Cells. 2008;26:2042-51 pubmed publisher
Ema H, Morita Y, Yamazaki S, Matsubara A, Seita J, Tadokoro Y, et al. Adult mouse hematopoietic stem cells: purification and single-cell assays. Nat Protoc. 2006;1:2979-87 pubmed
Jung Y, Wang J, Song J, Shiozawa Y, Wang J, Havens A, et al. Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation. Blood. 2007;110:82-90 pubmed
Jordan M, Fletcher J, Pellicci D, Baxter A. Slamf1, the NKT cell control gene Nkt1. J Immunol. 2007;178:1618-27 pubmed
Fraser S, Isern J, Baron M. Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression. Blood. 2007;109:343-52 pubmed
Kim I, He S, Yilmaz O, Kiel M, Morrison S. Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors. Blood. 2006;108:737-44 pubmed
Forsberg E, Prohaska S, Katzman S, Heffner G, Stuart J, Weissman I. Differential expression of novel potential regulators in hematopoietic stem cells. PLoS Genet. 2005;1:e28 pubmed
product information
Antigen :
CD150
Apps. Abbrev. :
FC
Cat # :
115916
Clone :
TC15-12F12.2
Item :
Alexa Fluor® 488 anti-mouse CD150 (SLAM)
Isotype :
Rat IgG2a, λ
Other Names :
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Size :
100 μg
Price (USD) :
265 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A488
Immunogen :
Mouse SLAM-human IgG1 fusion protein
Application Notes :
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion^17, enhancing IFN-? production by Th1 cells when stimulated with CD3^1, and inhibiting CD3 induced T cell proliferation^6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA