product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse CD150 (SLAM)
catalog :
115901
quantity :
50 μg
price :
94 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
TC15-12F12.2
reactivity :
mouse
application :
immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 75
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse
Vavassori V, Mercuri E, Marcovecchio G, Castiello M, Schiroli G, Albano L, et al. Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome. EMBO Mol Med. 2021;13:e13545 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; loading ...
Ricci B, Tycksen E, Celik H, Belle J, Fontana F, Civitelli R, et al. Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 4a
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s9i
Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7c
Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig ex9j
Tikhonova A, Dolgalev I, Hu H, Sivaraj K, Hoxha E, Cuesta Dominguez A, et al. The bone marrow microenvironment at single-cell resolution. Nature. 2019;569:222-228 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig e8c
Jacome Galarza C, Percin G, Muller J, Mass E, Lazarov T, Eitler J, et al. Developmental origin, functional maintenance and genetic rescue of osteoclasts. Nature. 2019;568:541-545 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b, 6e
Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ev2g
Rao T, Gupta M, Softic S, Wang L, Jang Y, Thomou T, et al. Attenuation of PKCδ enhances metabolic activity and promotes expansion of blood progenitors. EMBO J. 2018;37: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Umemoto T, Hashimoto M, Matsumura T, Nakamura Ishizu A, Suda T. Ca2+-mitochondria axis drives cell division in hematopoietic stem cells. J Exp Med. 2018;215:2097-2113 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5g
Oda A, Tezuka T, Ueno Y, Hosoda S, Amemiya Y, Notsu C, et al. Niche-induced extramedullary hematopoiesis in the spleen is regulated by the transcription factor Tlx1. Sci Rep. 2018;8:8308 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1e
Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s1a
Chapple R, Hu T, Tseng Y, Liu L, Kitano A, Luu V, et al. ERα promotes murine hematopoietic regeneration through the Ire1α-mediated unfolded protein response. elife. 2018;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Lu Z, Hong C, Kong G, Assumpção A, Ong I, Bresnick E, et al. Polycomb Group Protein YY1 Is an Essential Regulator of Hematopoietic Stem Cell Quiescence. Cell Rep. 2018;22:1545-1559 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Mumau M, Vanderbeck A, Lynch E, Golec S, Emerson S, Punt J. Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol. 2018;200:1078-1087 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
  • flow cytometry; mouse; 1:100; fig 7
Miller M, Rosten P, Lemieux M, Lai C, Humphries R. Meis1 Is Required for Adult Mouse Erythropoiesis, Megakaryopoiesis and Hematopoietic Stem Cell Expansion. PLoS ONE. 2016;11:e0151584 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Leiva M, Quintana J, Ligos J, Hidalgo A. Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability. Nat Commun. 2016;7:10222 pubmed publisher
  • flow cytometry; mouse; fig 2
Choi Y, Gullicksrud J, Xing S, Zeng Z, Shan Q, Li F, et al. LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6. Nat Immunol. 2015;16:980-90 pubmed publisher
  • flow cytometry; mouse; fig 1
Rao T, Marks Bluth J, Sullivan J, Gupta M, Chandrakanthan V, Fitch S, et al. High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice. Stem Cell Res. 2015;14:307-22 pubmed publisher
  • flow cytometry; mouse; fig 1
Hu W, Dooley J, Chung S, Chandramohan D, Cimmino L, Mukherjee S, et al. miR-29a maintains mouse hematopoietic stem cell self-renewal by regulating Dnmt3a. Blood. 2015;125:2206-16 pubmed publisher
  • flow cytometry; mouse
CismaÅŸiu V, Popescu L. Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells. J Cell Mol Med. 2015;19:351-8 pubmed publisher
  • flow cytometry; mouse; fig 2
Krysiak K, Tibbitts J, Shao J, Liu T, Ndonwi M, Walter M. Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells. Exp Hematol. 2015;43:319-30.e10 pubmed publisher
  • flow cytometry; mouse; fig 3
Karamitros D, Patmanidi A, Kotantaki P, Potocnik A, Bähr Ivacevic T, Benes V, et al. Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors. Development. 2015;142:70-81 pubmed publisher
  • flow cytometry; mouse; fig 2
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
  • flow cytometry; mouse
Xu Y, Swartz K, Siu K, Bhattacharyya M, Minella A. Fbw7-dependent cyclin E regulation ensures terminal maturation of bone marrow erythroid cells by restraining oxidative metabolism. Oncogene. 2014;33:3161-71 pubmed publisher
  • flow cytometry; mouse
Kerenyi M, Shao Z, Hsu Y, Guo G, Luc S, O Brien K, et al. Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. elife. 2013;2:e00633 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
  • flow cytometry; mouse
Yvan Charvet L, Pagler T, Gautier E, Avagyan S, Siry R, Han S, et al. ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation. Science. 2010;328:1689-93 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Tummers B, Mari L, Guy C, Heckmann B, Rodriguez D, Rühl S, et al. Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis. Immunity. 2020;52:994-1006.e8 pubmed publisher
Frodermann V, Rohde D, Courties G, Severe N, Schloss M, Amatullah H, et al. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nat Med. 2019;25:1761-1771 pubmed publisher
Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski A, Singhi N, et al. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Mol Ther. 2019;: pubmed publisher
Nachmani D, Bothmer A, Grisendi S, Mele A, Bothmer D, Lee J, et al. Germline NPM1 mutations lead to altered rRNA 2'-O-methylation and cause dyskeratosis congenita. Nat Genet. 2019;51:1518-1529 pubmed publisher
Hu J, Wu J, Li Y, Wang Z, Tang J, Li Z, et al. Sclerostin domain-containing protein 1 is dispensable for the differentiation of follicular helper and follicular regulatory T cells during acute viral infection. Am J Transl Res. 2019;11:3722-3736 pubmed
Ho Y, del Toro R, Rivera Torres J, Rak J, Korn C, García García A, et al. Remodeling of Bone Marrow Hematopoietic Stem Cell Niches Promotes Myeloid Cell Expansion during Premature or Physiological Aging. Cell Stem Cell. 2019;25:407-418.e6 pubmed publisher
Nakagawa M, RATHINAM C. Constitutive Activation of the Canonical NF-κB Pathway Leads to Bone Marrow Failure and Induction of Erythroid Signature in Hematopoietic Stem Cells. Cell Rep. 2018;25:2094-2109.e4 pubmed publisher
Hsu J, Dayaram T, Tovy A, De Braekeleer E, Jeong M, Wang F, et al. PPM1D Mutations Drive Clonal Hematopoiesis in Response to Cytotoxic Chemotherapy. Cell Stem Cell. 2018;23:700-713.e6 pubmed publisher
Gubin M, Esaulova E, Ward J, Malkova O, Runci D, Wong P, et al. High-Dimensional Analysis Delineates Myeloid and Lymphoid Compartment Remodeling during Successful Immune-Checkpoint Cancer Therapy. Cell. 2018;175:1014-1030.e19 pubmed publisher
Bellelli R, Borel V, Logan C, Svendsen J, Cox D, Nye E, et al. Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell. 2018;70:707-721.e7 pubmed publisher
Jiang L, Han X, Wang J, Wang C, Sun X, Xie J, et al. SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling. J Exp Med. 2018;215:1337-1347 pubmed publisher
Patel R, Qing Y, Kennedy L, Yan Y, Pink J, Aguila B, et al. MMR Deficiency Does Not Sensitize or Compromise the Function of Hematopoietic Stem Cells to Low and High LET Radiation. Stem Cells Transl Med. 2018;7:513-520 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, et al. A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018;48:688-701.e7 pubmed publisher
Seike M, Omatsu Y, Watanabe H, Kondoh G, Nagasawa T. Stem cell niche-specific Ebf3 maintains the bone marrow cavity. Genes Dev. 2018;32:359-372 pubmed publisher
Tusi B, Wolock S, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, et al. Population snapshots predict early haematopoietic and erythroid hierarchies. Nature. 2018;555:54-60 pubmed publisher
Zhang H, Li H, Hillmer E, Zhao Y, Chrisikos T, Hu H, et al. Genetic rescue of lineage-balanced blood cell production reveals a crucial role for STAT3 antiinflammatory activity in hematopoiesis. Proc Natl Acad Sci U S A. 2018;115:E2311-E2319 pubmed publisher
McKenney A, Lau A, Somasundara A, Spitzer B, Intlekofer A, Ahn J, et al. JAK2/IDH-mutant-driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. J Clin Invest. 2018;128:789-804 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Cole C, Russler Germain D, Ketkar S, Verdoni A, Smith A, Bangert C, et al. Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. J Clin Invest. 2017;127:3657-3674 pubmed publisher
Umemoto T, Matsuzaki Y, Shiratsuchi Y, Hashimoto M, Yoshimoto T, Nakamura Ishizu A, et al. Integrin αvβ3 enhances the suppressive effect of interferon-γ on hematopoietic stem cells. EMBO J. 2017;36:2390-2403 pubmed publisher
Gullicksrud J, Li F, Xing S, Zeng Z, Peng W, Badovinac V, et al. Differential Requirements for Tcf1 Long Isoforms in CD8+ and CD4+ T Cell Responses to Acute Viral Infection. J Immunol. 2017;199:911-919 pubmed publisher
Shan Q, Zeng Z, Xing S, Li F, Hartwig S, Gullicksrud J, et al. The transcription factor Runx3 guards cytotoxic CD8+ effector T cells against deviation towards follicular helper T cell lineage. Nat Immunol. 2017;18:931-939 pubmed publisher
Anso E, Weinberg S, Diebold L, Thompson B, Malinge S, Schumacker P, et al. The mitochondrial respiratory chain is essential for haematopoietic stem cell function. Nat Cell Biol. 2017;19:614-625 pubmed publisher
Lefrançais E, Ortiz Muñoz G, Caudrillier A, Mallavia B, Liu F, Sayah D, et al. The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors. Nature. 2017;544:105-109 pubmed publisher
Kramer A, Kothari A, Wilson W, Celik H, Nikitas J, Mallaney C, et al. Dnmt3a regulates T-cell development and suppresses T-ALL transformation. Leukemia. 2017;31:2479-2490 pubmed publisher
Pleines I, Woods J, Chappaz S, Kew V, Foad N, Ballester Beltrán J, et al. Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia. J Clin Invest. 2017;127:814-829 pubmed publisher
Nish S, Zens K, Kratchmarov R, Lin W, Adams W, Chen Y, et al. CD4+ T cell effector commitment coupled to self-renewal by asymmetric cell divisions. J Exp Med. 2017;214:39-47 pubmed publisher
Morita Y, Ema H, Nakauchi H. Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment. J Exp Med. 2010;207:1173-82 pubmed publisher
Song J, Kiel M, Wang Z, Wang J, Taichman R, Morrison S, et al. An in vivo model to study and manipulate the hematopoietic stem cell niche. Blood. 2010;115:2592-600 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
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Ema H, Morita Y, Yamazaki S, Matsubara A, Seita J, Tadokoro Y, et al. Adult mouse hematopoietic stem cells: purification and single-cell assays. Nat Protoc. 2006;1:2979-87 pubmed
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McCausland M, Yusuf I, Tran H, Ono N, Yanagi Y, Crotty S. SAP regulation of follicular helper CD4 T cell development and humoral immunity is independent of SLAM and Fyn kinase. J Immunol. 2007;178:817-28 pubmed
Umemoto T, Yamato M, Shiratsuchi Y, Terasawa M, Yang J, Nishida K, et al. Expression of Integrin beta3 is correlated to the properties of quiescent hemopoietic stem cells possessing the side population phenotype. J Immunol. 2006;177:7733-9 pubmed
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Kim I, He S, Yilmaz O, Kiel M, Morrison S. Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors. Blood. 2006;108:737-44 pubmed
product information
Antigen :
CD150
Apps. Abbrev. :
FC, IP
Cat # :
115901
Clone :
TC15-12F12.2
Item :
Purified anti-mouse CD150 (SLAM)
Isotype :
Rat IgG2a, λ
Other Names :
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Size :
50 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
Mouse SLAM-human IgG1 fusion protein
Application Notes :
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion^17, enhancing IFN-? production by Th1 cells when stimulated with CD3^1, and inhibiting CD3 induced T cell proliferation^6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA