product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-mouse CD45.1
catalog :
110727
quantity :
25 μg
price :
118 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP/Cy5.5
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Trapecar M, Khan S, Cohn B, Wu F, Sanjabi S. B cells are the predominant mediators of early systemic viral dissemination during rectal LCMV infection. Mucosal Immunol. 2018;11:1158-1167 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Pleuger C, Ai D, Hoppe M, Winter L, Bohnert D, Karl D, et al. The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis. elife. 2022;11: pubmed publisher
Wang Y, Wang Y, Ding L, Ren X, Wang B, Wang L, et al. Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages. Cell Rep. 2022;41:111738 pubmed publisher
Lim J, Sargur Madabushi S, Vishwasrao P, Song J, Abdelhamid A, Ghimire H, et al. Total marrow irradiation reduces organ damage and enhances tissue repair with the potential to increase the targeted dose of bone marrow in both young and old mice. Front Oncol. 2022;12:1045016 pubmed publisher
Melzi E, Willis J, Ma K, Lin Y, Kratochvil S, Berndsen Z, et al. Membrane-bound mRNA immunogens lower the threshold to activate HIV Env V2 apex-directed broadly neutralizing B cell precursors in humanized mice. Immunity. 2022;55:2168-2186.e6 pubmed publisher
Lamb T, Muthumalage T, Meehan Atrash J, Rahman I. Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner. Toxics. 2022;10: pubmed publisher
Tas J, Koo J, Lin Y, Xie Z, Steichen J, Jackson A, et al. Antibodies from primary humoral responses modulate the recruitment of naive B cells during secondary responses. Immunity. 2022;55:1856-1871.e6 pubmed publisher
Brog R, Ferry S, Schiebout C, Messier C, Cook W, Abdullah L, et al. Superkine IL2 and IL33 armored CAR T cells reshape the tumor microenvironment and reduce growth of multiple solid tumors. Cancer Immunol Res. 2022;: pubmed publisher
Ma K, Sun L, Shen M, Zhang X, Xiao Z, Wang J, et al. Functional assessment of the cell-autonomous role of NADase CD38 in regulating CD8+ T cell exhaustion. iScience. 2022;25:104347 pubmed publisher
Reticker Flynn N, Zhang W, Belk J, Basto P, Escalante N, Pilarowski G, et al. Lymph node colonization induces tumor-immune tolerance to promote distant metastasis. Cell. 2022;185:1924-1942.e23 pubmed publisher
Li X, Wang H, Yu X, Saha G, Kalafati L, Ioannidis C, et al. Maladaptive innate immune training of myelopoiesis links inflammatory comorbidities. Cell. 2022;185:1709-1727.e18 pubmed publisher
Mujal A, Combes A, Rao A, Binnewies M, Samad B, TSUI J, et al. Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer. Cancer Immunol Res. 2022;10:403-419 pubmed publisher
Sharma S, Wang Q, Muthumalage T, Rahman I. Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke. Pathophysiology. 2021;28:501-512 pubmed publisher
Duan Q, Ding J, Li F, Liu X, Zhao Y, Yu H, et al. Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation. Front Cell Dev Biol. 2021;9:761193 pubmed publisher
Russler Germain E, Jung J, Miller A, Young S, Yi J, Wehmeier A, et al. Commensal Cryptosporidium colonization elicits a cDC1-dependent Th1 response that promotes intestinal homeostasis and limits other infections. Immunity. 2021;54:2547-2564.e7 pubmed publisher
Beauchamp E, Leventhal M, Bernard E, Hoppe E, Todisco G, Creignou M, et al. ZBTB33 is mutated in clonal hematopoiesis and myelodysplastic syndromes and impacts RNA splicing. Blood Cancer Discov. 2021;2:500-517 pubmed publisher
Zong L, Tanaka Yano M, Park B, Yanai H, Turhan F, Croteau D, et al. NAD+ augmentation with nicotinamide riboside improves lymphoid potential of Atm-/- and old mice HSCs. NPJ Aging Mech Dis. 2021;7:25 pubmed publisher
Glaros V, Rauschmeier R, Artemov A, Reinhardt A, Ols S, Emmanouilidi A, et al. Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response. Immunity. 2021;54:2005-2023.e10 pubmed publisher
Hirano K, Hosokawa H, Koizumi M, Endo Y, Yahata T, Ando K, et al. LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice. elife. 2021;10: pubmed publisher
Di Pilato M, Kfuri Rubens R, Pruessmann J, Ozga A, Messemaker M, Cadilha B, et al. CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment. Cell. 2021;: pubmed publisher
Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
Inoue D, Polaski J, Taylor J, Castel P, Chen S, Kobayashi S, et al. Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Nat Genet. 2021;53:707-718 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Ehrens A, Lenz B, Neumann A, Giarrizzo S, Reichwald J, Frohberger S, et al. Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner. Cell Rep. 2021;34:108621 pubmed publisher
Murakami K, Kurotaki D, Kawase W, Soma S, Fukuchi Y, Kunimoto H, et al. OGT Regulates Hematopoietic Stem Cell Maintenance via PINK1-Dependent Mitophagy. Cell Rep. 2021;34:108579 pubmed publisher
Wang X, Ray R, Kratochvil S, Melzi E, Lin Y, Giguere S, et al. Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors. EMBO J. 2021;40:e105926 pubmed publisher
Hirai T, Yang Y, Zenke Y, Li H, Chaudhri V, De La Cruz Diaz J, et al. Competition for Active TGFβ Cytokine Allows for Selective Retention of Antigen-Specific Tissue- Resident Memory T Cells in the Epidermal Niche. Immunity. 2021;54:84-98.e5 pubmed publisher
Huang D, Tran J, Olson A, Vollbrecht T, Tenuta M, Guryleva M, et al. Vaccine elicitation of HIV broadly neutralizing antibodies from engineered B cells. Nat Commun. 2020;11:5850 pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Zeis P, Lian M, Fan X, Herman J, Hernandez D, Gentek R, et al. In Situ Maturation and Tissue Adaptation of Type 2 Innate Lymphoid Cell Progenitors. Immunity. 2020;53:775-792.e9 pubmed publisher
Evgin L, Huff A, Wongthida P, Thompson J, Kottke T, Tonne J, et al. Oncolytic virus-derived type I interferon restricts CAR T cell therapy. Nat Commun. 2020;11:3187 pubmed publisher
Bayık D, Zhou Y, Park C, Hong C, Vail D, Silver D, et al. Myeloid-Derived Suppressor Cell Subsets Drive Glioblastoma Growth in a Sex-Specific Manner. Cancer Discov. 2020;10:1210-1225 pubmed publisher
Liang Y, Yi P, Wang X, Zhang B, Jie Z, Soong L, et al. Retinoic Acid Modulates Hyperactive T Cell Responses and Protects Vitamin A-Deficient Mice against Persistent Lymphocytic Choriomeningitis Virus Infection. J Immunol. 2020;204:2984-2994 pubmed publisher
Yang W, Gibson S, Yan Z, Wei H, Tao J, Sha B, et al. Protein kinase 2 (CK2) controls CD4+ T cell effector function in the pathogenesis of colitis. Mucosal Immunol. 2020;: pubmed publisher
Moutuou M, Gauthier S, Chen N, LeBoeuf D, Guimond M. Studying Peripheral T Cell Homeostasis in Mice: A Concise Technical Review. Methods Mol Biol. 2020;2111:267-283 pubmed publisher
Kretschmer L, Flossdorf M, Mir J, Cho Y, Plambeck M, Treise I, et al. Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nat Commun. 2020;11:113 pubmed publisher
Muri J, Thut H, Bornkamm G, Kopf M. B1 and Marginal Zone B Cells but Not Follicular B2 Cells Require Gpx4 to Prevent Lipid Peroxidation and Ferroptosis. Cell Rep. 2019;29:2731-2744.e4 pubmed publisher
Kim C, Jadhav R, Gustafson C, Smithey M, Hirsch A, Uhrlaub J, et al. Defects in Antiviral T Cell Responses Inflicted by Aging-Associated miR-181a Deficiency. Cell Rep. 2019;29:2202-2216.e5 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
Sangesland M, Ronsard L, Kazer S, Bals J, Boyoglu Barnum S, Yousif A, et al. Germline-Encoded Affinity for Cognate Antigen Enables Vaccine Amplification of a Human Broadly Neutralizing Response against Influenza Virus. Immunity. 2019;51:735-749.e8 pubmed publisher
Krueger C, Thoms F, Keller E, Vogel M, Bachmann M. Virus-Specific Secondary Plasma Cells Produce Elevated Levels of High-Avidity Antibodies but Are Functionally Short Lived. Front Immunol. 2019;10:1831 pubmed publisher
Liu D, Yin X, Olyha S, Nascimento M, Chen P, White T, et al. IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α+ Dendritic Cells Promotes Listeria monocytogenes Infection. Immunity. 2019;: pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Srivastava S, Salter A, Liggitt D, Yechan Gunja S, Sarvothama M, Cooper K, et al. Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting. Cancer Cell. 2019;35:489-503.e8 pubmed publisher
Turner J, Ke F, GRIGOROVA I. B Cell Receptor Crosslinking Augments Germinal Center B Cell Selection when T Cell Help Is Limiting. Cell Rep. 2018;25:1395-1403.e4 pubmed publisher
Marcus A, Mao A, Lensink Vasan M, Wang L, Vance R, Raulet D. Tumor-Derived cGAMP Triggers a STING-Mediated Interferon Response in Non-tumor Cells to Activate the NK Cell Response. Immunity. 2018;49:754-763.e4 pubmed publisher
Stewart I, Radtke D, Phillips B, McGowan S, Bannard O. Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are Damaging. Immunity. 2018;49:477-489.e7 pubmed publisher
Zhu Y, Padgett L, Dinh H, Marcovecchio P, Blatchley A, Wu R, et al. Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow. Cell Rep. 2018;24:2329-2341.e8 pubmed publisher
Lee S, North K, Kim E, Jang E, Obeng E, Lu S, et al. Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations. Cancer Cell. 2018;34:225-241.e8 pubmed publisher
Suan D, Kräutler N, Mååg J, Butt D, Bourne K, Hermes J, et al. CCR6 Defines Memory B Cell Precursors in Mouse and Human Germinal Centers, Revealing Light-Zone Location and Predominant Low Antigen Affinity. Immunity. 2017;47:1142-1153.e4 pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Yuan X, Yang B, Dong Y, Yamamura A, Fu W. CRIg, a tissue-resident macrophage specific immune checkpoint molecule, promotes immunological tolerance in NOD mice, via a dual role in effector and regulatory T cells. elife. 2017;6: pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
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Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
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product information
Antigen :
CD45.1
Apps. Abbrev. :
FC
Cat # :
110727
Clone :
A20
Item :
PerCP/Cyanine5.5 anti-mouse CD45.1
Isotype :
Mouse (A.SW) IgG2a, κ
Other Names :
T200, Ly-5.1, LCA
Size :
25 μg
Price (USD) :
118 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
SJL mouse thymocytes and splenocytes
Application Notes :
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^3, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded^7 and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA