product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse CD45.1
catalog :
110721
quantity :
25 μg
price :
94 USD
clonality :
monoclonal
host :
mouse
conjugate :
Pacific Blue
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 75
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2a, 3b
Koutn xed k J, Klepsch V, Pommermayr M, Thuille N, Baier G, Siegmund K. A MLR-Based Approach to Analyze Regulators of T Lymphocyte Activation In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
  • flow cytometry; mouse; loading ...; fig e5h
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5f
Yang C, Kwon D, Kim M, Im S, Lee Y. Commensal Microbiome Expands Tγδ17 Cells in the Lung and Promotes Particulate Matter-Induced Acute Neutrophilia. Front Immunol. 2021;12:645741 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Page N, Lemeille S, Vincenti I, Klimek B, Mariotte A, Wagner I, et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. Nat Commun. 2021;12:1009 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s3-1b
Kalinski A, Yoon C, Huffman L, Duncker P, Kohen R, Passino R, et al. Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s10b
Lee J, Stone M, Porrett P, Thomas S, Komar C, Li J, et al. Hepatocytes direct the formation of a pro-metastatic niche in the liver. Nature. 2019;567:249-252 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s13f
Stivala S, Codilupi T, Brkic S, Baerenwaldt A, Ghosh N, Hao Shen H, et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest. 2019;130:1596-1611 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a, 3b
Maseda D, Banerjee A, Johnson E, Washington M, Kim H, Lau K, et al. mPGES-1-Mediated Production of PGE2 and EP4 Receptor Sensing Regulate T Cell Colonic Inflammation. Front Immunol. 2018;9:2954 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Ximerakis M, Holton K, Giadone R, Ozek C, Saxena M, Santiago S, et al. Heterochronic parabiosis reprograms the mouse brain transcriptome by shifting aging signatures in multiple cell types. Nat Aging. 2023;3:327-345 pubmed publisher
Le D, Florez M, Kus P, Tran B, Kain B, Zhu Y, et al. BATF2 promotes HSC myeloid differentiation by amplifying IFN response mediators during chronic infection. iScience. 2023;26:106059 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Cheng H, Qiu Y, Xu Y, Chen L, Ma K, Tao M, et al. Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness. Nat Metab. 2023;5:314-330 pubmed publisher
Kasuya T, Tanaka S, Tamura J, Etori K, Shoda J, Hattori K, et al. Epithelial cell-derived cytokine TSLP activates regulatory T cells by enhancing fatty acid uptake. Sci Rep. 2023;13:1653 pubmed publisher
Lombard Vadnais F, Chabot Roy G, Zahn A, Rodriguez Torres S, Di Noia J, Melichar H, et al. Activation-induced cytidine deaminase expression by thymic B cells promotes T-cell tolerance and limits autoimmunity. iScience. 2023;26:105852 pubmed publisher
Koutn xed k J, Leitges M, Siegmund K. T cell-intrinsic protein kinase D3 is dispensable for the cells' activation. Front Immunol. 2022;13:1049033 pubmed publisher
Hodgson R, Xu X, Anzilotti C, Deobagkar Lele M, Crockford T, Kepple J, et al. NDRG1 is induced by antigen-receptor signaling but dispensable for B and T cell self-tolerance. Commun Biol. 2022;5:1216 pubmed publisher
Iberg C, Bourque J, Fallahee I, Son S, Hawiger D. TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell Rep. 2022;39:110657 pubmed publisher
MacLean A, Richmond N, Koneva L, Attar M, Medina C, Thornton E, et al. Secondary influenza challenge triggers resident memory B cell migration and rapid relocation to boost antibody secretion at infected sites. Immunity. 2022;55:718-733.e8 pubmed publisher
Müller M, Gräbnitz F, Barandun N, Shen Y, Wendt F, Steiner S, et al. Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks. Nat Commun. 2021;12:7036 pubmed publisher
Chen X, MacNabb B, Flood B, Blazar B, Kline J. Divergent fates of antigen-specific CD8+ T cell clones in mice with acute leukemia. Cell Rep. 2021;37:109991 pubmed publisher
Bates P, Rakhmilevich A, Cho M, Bouchlaka M, Rao S, Hales J, et al. Combining Immunocytokine and Ex Vivo Activated NK Cells as a Platform for Enhancing Graft-Versus-Tumor Effects Against GD2+ Murine Neuroblastoma. Front Immunol. 2021;12:668307 pubmed publisher
Pack A, Schwartzhoff P, Zacharias Z, Fernández Ruiz D, Heath W, Gurung P, et al. Hemozoin-mediated inflammasome activation limits long-lived anti-malarial immunity. Cell Rep. 2021;36:109586 pubmed publisher
Liu X, Yu J, Xu L, Umphred Wilson K, Peng F, Ding Y, et al. Notch-induced endoplasmic reticulum-associated degradation governs mouse thymocyte β-selection. elife. 2021;10: pubmed publisher
Cheng Y, Xie W, Pickering B, Chu K, Savino A, Yang X, et al. N6-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation. Cancer Cell. 2021;39:958-972.e8 pubmed publisher
Hormaechea Agulla D, Matatall K, Le D, Kain B, Long X, Kus P, et al. Chronic infection drives Dnmt3a-loss-of-function clonal hematopoiesis via IFNγ signaling. Cell Stem Cell. 2021;28:1428-1442.e6 pubmed publisher
Rodriguez Garcia A, Lynn R, Poussin M, Eiva M, Shaw L, O Connor R, et al. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy. Nat Commun. 2021;12:877 pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
Florez M, Matatall K, Jeong Y, Ortinau L, Shafer P, Lynch A, et al. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2. Cell Rep. 2020;33:108530 pubmed publisher
Sandu I, Cerletti D, Claassen M, Oxenius A. Exhausted CD8+ T cells exhibit low and strongly inhibited TCR signaling during chronic LCMV infection. Nat Commun. 2020;11:4454 pubmed publisher
Liu X, Boyer M, Holmgren A, Shin S. Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation. Cell Host Microbe. 2020;28:683-698.e6 pubmed publisher
de Laval B, Maurizio J, Kandalla P, Brisou G, Simonnet L, Huber C, et al. C/EBPβ-Dependent Epigenetic Memory Induces Trained Immunity in Hematopoietic Stem Cells. Cell Stem Cell. 2020;26:657-674.e8 pubmed publisher
Kretschmer L, Flossdorf M, Mir J, Cho Y, Plambeck M, Treise I, et al. Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nat Commun. 2020;11:113 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Roco J, Mesin L, Binder S, Nefzger C, Gonzalez Figueroa P, Canete P, et al. Class-Switch Recombination Occurs Infrequently in Germinal Centers. Immunity. 2019;51:337-350.e7 pubmed publisher
Fiege J, Stone I, Fay E, Markman M, Wijeyesinghe S, Macchietto M, et al. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019;: pubmed publisher
Goldstein J, Tabebordbar M, Zhu K, Wang L, Messemer K, Peacker B, et al. In Situ Modification of Tissue Stem and Progenitor Cell Genomes. Cell Rep. 2019;27:1254-1264.e7 pubmed publisher
Srivastava S, Salter A, Liggitt D, Yechan Gunja S, Sarvothama M, Cooper K, et al. Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting. Cancer Cell. 2019;35:489-503.e8 pubmed publisher
Marcandalli J, Fiala B, Ols S, Perotti M, De van der Schueren W, Snijder J, et al. Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus. Cell. 2019;176:1420-1431.e17 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Turner J, Ke F, GRIGOROVA I. B Cell Receptor Crosslinking Augments Germinal Center B Cell Selection when T Cell Help Is Limiting. Cell Rep. 2018;25:1395-1403.e4 pubmed publisher
Bouziat R, Biering S, Kouame E, Sangani K, Kang S, Ernest J, et al. Murine Norovirus Infection Induces TH1 Inflammatory Responses to Dietary Antigens. Cell Host Microbe. 2018;24:677-688.e5 pubmed publisher
Diao J, Gu H, Tang M, Zhao J, Cattral M. Tumor Dendritic Cells (DCs) Derived from Precursors of Conventional DCs Are Dispensable for Intratumor CTL Responses. J Immunol. 2018;201:1306-1314 pubmed publisher
Wünsche P, Eckert E, Holland Letz T, Paruzynski A, Hotz Wagenblatt A, Fronza R, et al. Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs. Cell Stem Cell. 2018;23:132-146.e9 pubmed publisher
Lu Y, Wang Q, Xue G, Bi E, Ma X, Wang A, et al. Th9 Cells Represent a Unique Subset of CD4+ T Cells Endowed with the Ability to Eradicate Advanced Tumors. Cancer Cell. 2018;33:1048-1060.e7 pubmed publisher
Page N, Klimek B, De Roo M, Steinbach K, Soldati H, Lemeille S, et al. Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells. Immunity. 2018;48:937-950.e8 pubmed publisher
Li J, Lee Y, Li Y, Jiang Y, Lu H, Zang W, et al. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells. Immunity. 2018;48:773-786.e5 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Yáñez A, Coetzee S, Olsson A, Muench D, Berman B, Hazelett D, et al. Granulocyte-Monocyte Progenitors and Monocyte-Dendritic Cell Progenitors Independently Produce Functionally Distinct Monocytes. Immunity. 2017;47:890-902.e4 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Hamilton J, Wu Q, Yang P, Luo B, Liu S, Hong H, et al. Cutting Edge: Endogenous IFN-β Regulates Survival and Development of Transitional B Cells. J Immunol. 2017;199:2618-2623 pubmed publisher
Kosack L, Gawish R, Lercher A, Vilagos B, Hladik A, Lakovits K, et al. The lipid-sensor TREM2 aggravates disease in a model of LCMV-induced hepatitis. Sci Rep. 2017;7:11289 pubmed publisher
Zhang Y, Kurupati R, Liu L, Zhou X, Zhang G, Hudaihed A, et al. Enhancing CD8+ T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer Cell. 2017;32:377-391.e9 pubmed publisher
Oliveira A, Gomes Neto J, Barbosa C, Granato A, Reis B, Santos B, et al. Crucial role for T cell-intrinsic IL-18R-MyD88 signaling in cognate immune response to intracellular parasite infection. elife. 2017;6: pubmed publisher
Sabouri Z, Perotti S, Spierings E, Humburg P, Yabas M, Bergmann H, et al. IgD attenuates the IgM-induced anergy response in transitional and mature B cells. Nat Commun. 2016;7:13381 pubmed publisher
Onodera T, Hosono A, Odagiri T, Tashiro M, Kaminogawa S, Okuno Y, et al. Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling. J Immunol. 2016;196:4172-84 pubmed publisher
Wigton E, Thompson S, Long R, Jacobelli J. Myosin-IIA regulates leukemia engraftment and brain infiltration in a mouse model of acute lymphoblastic leukemia. J Leukoc Biol. 2016;100:143-53 pubmed publisher
Kitano M, Yamazaki C, Takumi A, Ikeno T, Hemmi H, Takahashi N, et al. Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node. Proc Natl Acad Sci U S A. 2016;113:1044-9 pubmed publisher
Siggs O, Popkin D, Krebs P, Li X, Tang M, Zhan X, et al. Mutation of the ER retention receptor KDELR1 leads to cell-intrinsic lymphopenia and a failure to control chronic viral infection. Proc Natl Acad Sci U S A. 2015;112:E5706-14 pubmed publisher
Calderon B, Carrero J, Ferris S, Sojka D, Moore L, Epelman S, et al. The pancreas anatomy conditions the origin and properties of resident macrophages. J Exp Med. 2015;212:1497-512 pubmed publisher
Minamitani T, Yasui T, Ma Y, Zhou H, Okuzaki D, Tsai C, et al. Evasion of affinity-based selection in germinal centers by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A. 2015;112:11612-7 pubmed publisher
Nadrah K, Beck T, Pereira J. Immature B Cell Egress from Bone Marrow Is SOCS3 Independent. PLoS ONE. 2015;10:e0136061 pubmed publisher
Mills R, Lam V, Tan A, Cresalia N, Oksenberg N, Zikherman J, et al. Unbiased modifier screen reveals that signal strength determines the regulatory role murine TLR9 plays in autoantibody production. J Immunol. 2015;194:3675-86 pubmed publisher
Lin G, Snell L, Wortzman M, Clouthier D, Watts T. GITR-dependent regulation of 4-1BB expression: implications for T cell memory and anti-4-1BB-induced pathology. J Immunol. 2013;190:4627-39 pubmed publisher
Li L, Goetz C, Katerndahl C, Sakaguchi N, Farrar M. A Flt3- and Ras-dependent pathway primes B cell development by inducing a state of IL-7 responsiveness. J Immunol. 2010;184:1728-36 pubmed publisher
Withers D, Jaensson E, Gaspal F, McConnell F, Eksteen B, Anderson G, et al. The survival of memory CD4+ T cells within the gut lamina propria requires OX40 and CD30 signals. J Immunol. 2009;183:5079-84 pubmed publisher
Takada K, Jameson S. Self-class I MHC molecules support survival of naive CD8 T cells, but depress their functional sensitivity through regulation of CD8 expression levels. J Exp Med. 2009;206:2253-69 pubmed publisher
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Beamer C, Holian A. Antigen-presenting cell population dynamics during murine silicosis. Am J Respir Cell Mol Biol. 2007;37:729-38 pubmed
Pascal V, Nathan N, Claudio E, Siebenlist U, Anderson S. NF-kappa B p50/p65 affects the frequency of Ly49 gene expression by NK cells. J Immunol. 2007;179:1751-9 pubmed
product information
Antigen :
CD45.1
Apps. Abbrev. :
FC
Cat # :
110721
Clone :
A20
Item :
Pacific Blue™ anti-mouse CD45.1
Isotype :
Mouse (A.SW) IgG2a, κ
Other Names :
T200, Ly-5.1, LCA
Size :
25 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PB
Immunogen :
SJL mouse thymocytes and splenocytes
Application Notes :
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^3, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded^7 and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA