product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD45.1
catalog :
110715
quantity :
25 μg
price :
110 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2a, 3b
Koutn xed k J, Klepsch V, Pommermayr M, Thuille N, Baier G, Siegmund K. A MLR-Based Approach to Analyze Regulators of T Lymphocyte Activation In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5s4
La Rosa C, Cavallo F, Pecora A, Chincarini M, Ala U, Faulkes C, et al. Phylogenetic variation in cortical layer II immature neuron reservoir of mammals. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5g,
Sun D, Zhang M, Sun P, Liu G, Strickland A, Chen Y, et al. VCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection. PLoS Pathog. 2020;16:e1008361 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Polonsky M, Rimer J, Kern Perets A, Zaretsky I, Miller S, Bornstein C, et al. Induction of CD4 T cell memory by local cellular collectivity. Science. 2018;360: pubmed publisher
  • flow cytometry; mouse; tbl s3
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Ernst M, Pronk E, van Dijk C, van Strien P, van Tienhoven T, Wevers M, et al. Hematopoietic Cell Autonomous Disruption of Hematopoiesis in a Germline Loss-of-function Mouse Model of RUNX1-FPD. Hemasphere. 2023;7:e824 pubmed publisher
Zhao M, Shao F, Yu D, Zhang J, Liu Z, Ma J, et al. Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis. Nat Commun. 2022;13:7600 pubmed publisher
VanDyke D, Iglesias M, Tomala J, Young A, Smith J, Perry J, et al. Engineered human cytokine/antibody fusion proteins expand regulatory T cells and confer autoimmune disease protection. Cell Rep. 2022;41:111478 pubmed publisher
Zhu Y, Gu H, Yang L, Li N, Chen Q, Kang D, et al. Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clin Transl Med. 2022;12:e887 pubmed publisher
Pulous F, Cruz Hern xe1 ndez J, Yang C, Kaya x, Paccalet A, Wojtkiewicz G, et al. Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis. Nat Neurosci. 2022;25:567-576 pubmed publisher
Morris V, Wang D, Li Z, Marion W, Hughes T, Sousa P, et al. Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells. Cell Rep. 2022;39:110752 pubmed publisher
Wang D, Tanaka Yano M, Meader E, Kinney M, Morris V, Lummertz da Rocha E, et al. Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Cell Rep. 2022;39:110587 pubmed publisher
North K, Benbarche S, Liu B, Pangallo J, Chen S, Stahl M, et al. Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Nat Biotechnol. 2022;: pubmed publisher
Pinho S, Wei Q, Maryanovich M, Zhang D, Balandr xe1 n J, Pierce H, et al. VCAM1 confers innate immune tolerance on haematopoietic and leukaemic stem cells. Nat Cell Biol. 2022;24:290-298 pubmed publisher
Wang X, Jiao A, Sun L, Li W, Yang B, Su Y, et al. Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanisms. elife. 2022;11: pubmed publisher
Chen F, El Naccache D, Ponessa J, Lemenze A, Espinosa V, Wu W, et al. Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion. Cell Rep. 2022;38:110215 pubmed publisher
Jia X, Chua B, Loh L, Koutsakos M, Kedzierski L, Olshansky M, et al. High expression of CD38 and MHC class II on CD8+ T cells during severe influenza disease reflects bystander activation and trogocytosis. Clin Transl Immunology. 2021;10:e1336 pubmed publisher
De Simone G, Andreata F, Blériot C, Fumagalli V, Laura C, Garcia Manteiga J, et al. Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming. Immunity. 2021;54:2089-2100.e8 pubmed publisher
Izumi G, Nakano H, Nakano K, Whitehead G, Grimm S, Fessler M, et al. CD11b+ lung dendritic cells at different stages of maturation induce Th17 or Th2 differentiation. Nat Commun. 2021;12:5029 pubmed publisher
Shaikh H, Vargas J, Mokhtari Z, Jarick K, Ulbrich M, Mosca J, et al. Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract. Front Immunol. 2021;12:689896 pubmed publisher
Le Q, Castro S, Tang T, Loeb A, Hylkema T, McKay C, et al. Therapeutic Targeting of Mesothelin with Chimeric Antigen Receptor T Cells in Acute Myeloid Leukemia. Clin Cancer Res. 2021;27:5718-5730 pubmed publisher
Di Pilato M, Kfuri Rubens R, Pruessmann J, Ozga A, Messemaker M, Cadilha B, et al. CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment. Cell. 2021;: pubmed publisher
Chappaz S, McArthur K, Kealy L, Law C, Tailler M, Lane R, et al. Homeostatic apoptosis prevents competition-induced atrophy in follicular B cells. Cell Rep. 2021;36:109430 pubmed publisher
Sun L, Zhao X, Liu X, Zhong B, Tang H, Jin W, et al. Transcription factor Ascl2 promotes germinal center B cell responses by directly regulating AID transcription. Cell Rep. 2021;35:109188 pubmed publisher
Yang L, Li N, Yang D, Chen A, Tang J, Jing Y, et al. CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation. Cell Death Differ. 2021;: pubmed publisher
Chen Z, Arai E, Khan O, Zhang Z, Ngiow S, He Y, et al. In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer. Cell. 2021;184:1262-1280.e22 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Leimkühler N, Gleitz H, Ronghui L, Snoeren I, Fuchs S, Nagai J, et al. Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis. Cell Stem Cell. 2020;: pubmed publisher
Mogilenko D, Shpynov O, Andhey P, Arthur L, Swain A, Esaulova E, et al. Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK+ CD8+ T Cells as Conserved Hallmark of Inflammaging. Immunity. 2021;54:99-115.e12 pubmed publisher
Fu H, Sun Y, Shao Y, Saredy J, Cueto R, Liu L, et al. Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis. Front Immunol. 2020;11:595813 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
Frame J, Kubaczka C, Long T, Esain V, Soto R, Hachimi M, et al. Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production. Dev Cell. 2020;: pubmed publisher
Amor C, Feucht J, Leibold J, Ho Y, Zhu C, Alonso Curbelo D, et al. Senolytic CAR T cells reverse senescence-associated pathologies. Nature. 2020;583:127-132 pubmed publisher
Siamishi I, Iwanami N, Clapes T, Trompouki E, O Meara C, Boehm T. Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020;31:107756 pubmed publisher
Aegerter H, Kulikauskaite J, Crotta S, Patel H, Kelly G, Hessel E, et al. Influenza-induced monocyte-derived alveolar macrophages confer prolonged antibacterial protection. Nat Immunol. 2020;21:145-157 pubmed publisher
Ciecko A, Foda B, Barr J, Ramanathan S, Atkinson M, Serreze D, et al. Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation. Cell Rep. 2019;29:3073-3086.e5 pubmed publisher
Chen Z, Ji Z, Ngiow S, Manne S, Cai Z, Huang A, et al. TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision. Immunity. 2019;51:840-855.e5 pubmed publisher
Benechet A, De Simone G, Di Lucia P, Cilenti F, Barbiera G, Le Bert N, et al. Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming. Nature. 2019;574:200-205 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Miller B, Yates K, Gillis J, et al. PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019;20:1335-1347 pubmed publisher
Olson W, Jakic B, Labi V, Schoeler K, Kind M, Klepsch V, et al. Orphan Nuclear Receptor NR2F6 Suppresses T Follicular Helper Cell Accumulation through Regulation of IL-21. Cell Rep. 2019;28:2878-2891.e5 pubmed publisher
Jing Y, Kang D, Liu L, Huang H, Chen A, Yang L, et al. Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. J Allergy Clin Immunol. 2019;: pubmed publisher
Swidergall M, Solis N, Wang Z, Phan Q, Marshall M, Lionakis M, et al. EphA2 Is a Neutrophil Receptor for Candida albicans that Stimulates Antifungal Activity during Oropharyngeal Infection. Cell Rep. 2019;28:423-433.e5 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
Youshani A, Rowlston S, O Leary C, Forte G, Parker H, Liao A, et al. Non-myeloablative busulfan chimeric mouse models are less pro-inflammatory than head-shielded irradiation for studying immune cell interactions in brain tumours. J Neuroinflammation. 2019;16:25 pubmed publisher
Chen S, Fang L, Guo W, Zhou Y, Yu G, Li W, et al. Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells. Nat Commun. 2018;9:5298 pubmed publisher
Araujo Furlan C, Tosello Boari J, Rodríguez C, Canale F, Fiocca Vernengo F, Boccardo S, et al. Limited Foxp3+ Regulatory T Cells Response During Acute Trypanosoma cruzi Infection Is Required to Allow the Emergence of Robust Parasite-Specific CD8+ T Cell Immunity. Front Immunol. 2018;9:2555 pubmed publisher
Fan M, Low J, Tanimine N, Finn K, Priyadharshini B, Germana S, et al. Differential Roles of IL-2 Signaling in Developing versus Mature Tregs. Cell Rep. 2018;25:1204-1213.e4 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze L, et al. Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction. Immunity. 2017;47:1114-1128.e6 pubmed publisher
Jash A, Wang Y, Weisel F, Scharer C, Boss J, Shlomchik M, et al. ZBTB32 Restricts the Duration of Memory B Cell Recall Responses. J Immunol. 2016;197:1159-68 pubmed publisher
Belle J, Petrov J, Langlais D, Robert F, Cencic R, Shen S, et al. Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance. Cell Death Differ. 2016;23:759-75 pubmed publisher
Burgess M, Hwang E, Firestone A, Huang T, Xu J, Zuber J, et al. Preclinical efficacy of MEK inhibition in Nras-mutant AML. Blood. 2014;124:3947-55 pubmed publisher
Li L, Goetz C, Katerndahl C, Sakaguchi N, Farrar M. A Flt3- and Ras-dependent pathway primes B cell development by inducing a state of IL-7 responsiveness. J Immunol. 2010;184:1728-36 pubmed publisher
Withers D, Jaensson E, Gaspal F, McConnell F, Eksteen B, Anderson G, et al. The survival of memory CD4+ T cells within the gut lamina propria requires OX40 and CD30 signals. J Immunol. 2009;183:5079-84 pubmed publisher
Takada K, Jameson S. Self-class I MHC molecules support survival of naive CD8 T cells, but depress their functional sensitivity through regulation of CD8 expression levels. J Exp Med. 2009;206:2253-69 pubmed publisher
Lee S, Salek Ardakani S, Mittler R, Croft M. Hypercostimulation through 4-1BB distorts homeostasis of immune cells. J Immunol. 2009;182:6753-62 pubmed publisher
Hosoi A, Takeda Y, Furuichi Y, Kurachi M, Kimura K, Maekawa R, et al. Memory Th1 cells augment tumor-specific CTL following transcutaneous peptide immunization. Cancer Res. 2008;68:3941-9 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Beamer C, Holian A. Antigen-presenting cell population dynamics during murine silicosis. Am J Respir Cell Mol Biol. 2007;37:729-38 pubmed
Pascal V, Nathan N, Claudio E, Siebenlist U, Anderson S. NF-kappa B p50/p65 affects the frequency of Ly49 gene expression by NK cells. J Immunol. 2007;179:1751-9 pubmed
product information
Antigen :
CD45.1
Apps. Abbrev. :
FC
Cat # :
110715
Clone :
A20
Item :
APC/Cyanine7 anti-mouse CD45.1
Isotype :
Mouse (A.SW) IgG2a, κ
Other Names :
T200, Ly-5.1, LCA
Size :
25 μg
Price (USD) :
110 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
SJL mouse thymocytes and splenocytes
Application Notes :
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^3, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded^7 and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA