product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse CD45.1
catalog :
110714
quantity :
100 μg
price :
127 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 105
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4g
Tatsumi N, Codrington A, El Fenej J, Phondge V, Kumamoto Y. Effective CD4 T cell priming requires repertoire scanning by CD301b+ migratory cDC2 cells upon lymph node entry. Sci Immunol. 2021;6:eabg0336 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 8a, 8b
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig e9o
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Hou X, Chen G, Bracamonte Baran W, Choi H, Diny N, Sung J, et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep. 2019;28:172-189.e7 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4b
Tordesillas L, Lozano Ojalvo D, Dunkin D, Mondoulet L, Agudo J, Merad M, et al. PDL2+ CD11b+ dermal dendritic cells capture topical antigen through hair follicles to prime LAP+ Tregs. Nat Commun. 2018;9:5238 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mahr B, Pilat N, Granofszky N, Wiletel M, Muckenhuber M, Maschke S, et al. Hybrid resistance to parental bone marrow grafts in nonlethally irradiated mice. Am J Transplant. 2019;19:591-596 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3g
Pishesha N, Bilate A, Wibowo M, Huang N, Li Z, Deshycka R, et al. Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease. Proc Natl Acad Sci U S A. 2017;114:3157-3162 pubmed publisher
  • flow cytometry; mouse; tbl s1
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Oh J, Riek A, Bauerle K, Dusso A, McNerney K, Barve R, et al. Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes. Nat Commun. 2023;14:3278 pubmed publisher
Nixon B, Ji L, Li M. Assessing in vivo presentation of exogenous antigen in the tumor microenvironment in mice. STAR Protoc. 2023;4:102185 pubmed publisher
Yi C, Liu J, Deng W, Luo C, Qi J, Chen M, et al. Old age promotes retinal fibrosis in choroidal neovascularization through circulating fibrocytes and profibrotic macrophages. J Neuroinflammation. 2023;20:45 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Giannou A, Kempski J, Shiri A, L xfc cke J, Zhang T, Zhao L, et al. Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22. Immunity. 2023;56:125-142.e12 pubmed publisher
Tatsumi N, Davila Pagan A, Kumamoto Y. Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry. STAR Protoc. 2022;3:101845 pubmed publisher
Kraemer A, Sch xe4 fer A, Sprenger D, Sehnert B, Williams J, Luo A, et al. Impact of dietary vitamin D on immunoregulation and disease pathology in lupus-prone NZB/W F1 mice. Front Immunol. 2022;13:933191 pubmed publisher
Taylor D, Meyer C, Graves D, Sen R, Fu J, Tran E, et al. MuSyC dosing of adjuvanted cancer vaccines optimizes antitumor responses. Front Immunol. 2022;13:936129 pubmed publisher
Sun Y, Dotson G, Muir L, Ronquist S, Oravecz Wilson K, Peltier D, et al. Rearrangement of T Cell genome architecture regulates GVHD. iScience. 2022;25:104846 pubmed publisher
Kissiov D, Ethell A, Chen S, Wolf N, Zhang C, Dang S, et al. Binary outcomes of enhancer activity underlie stable random monoallelic expression. elife. 2022;11: pubmed publisher
Stanzione M, Zhong J, Wong E, LaSalle T, Wise J, Simoneau A, et al. Translesion DNA synthesis mediates acquired resistance to olaparib plus temozolomide in small cell lung cancer. Sci Adv. 2022;8:eabn1229 pubmed publisher
Iberg C, Bourque J, Fallahee I, Son S, Hawiger D. TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell Rep. 2022;39:110657 pubmed publisher
Parga Vidal L, Taggenbrock R, Beumer Chuwonpad A, Aglmous H, Kragten N, Behr F, et al. Hobit and Blimp-1 regulate TRM abundance after LCMV infection by suppressing tissue exit pathways of TRM precursors. Eur J Immunol. 2022;52:1095-1111 pubmed publisher
Bangs D, Tsitsiklis A, Steier Z, Chan S, Kaminski J, STREETS A, et al. CXCR3 regulates stem and proliferative CD8+ T cells during chronic infection by promoting interactions with DCs in splenic bridging channels. Cell Rep. 2022;38:110266 pubmed publisher
Kragten N, Taggenbrock R, Parga Vidal L, van Lier R, Stark R, van Gisbergen K. Hobit and Blimp-1 instruct the differentiation of iNKT cells into resident-phenotype lymphocytes after lineage commitment. Eur J Immunol. 2022;52:389-403 pubmed publisher
Durgin J, Thokala R, JOHNSON L, Song E, Leferovich J, Bhoj V, et al. Enhancing CAR T function with the engineered secretion of C. perfringens neuraminidase. Mol Ther. 2022;30:1201-1214 pubmed publisher
Wu J, Li G, Li D, Zhou J, Dong Z, Jiang P. Detection of CD8+ T cell-mediated immune responses to bacterial infection in mice. STAR Protoc. 2021;2:101022 pubmed publisher
Wang C, Cui A, Bukenya M, Aung A, Pradhan D, Whittaker C, et al. Reprogramming NK cells and macrophages via combined antibody and cytokine therapy primes tumors for elimination by checkpoint blockade. Cell Rep. 2021;37:110021 pubmed publisher
Endo Umeda K, Nakashima H, Uno S, Toyoshima S, Umeda N, Komine Aizawa S, et al. Liver X receptors regulate natural killer T cell population and antitumor activity in the liver of mice. Sci Rep. 2021;11:22595 pubmed publisher
Cohen K, Mouhadeb O, Ben Shlomo S, Langer M, Neumann A, Erez N, et al. COMMD10 is critical for Kupffer cell survival and controls Ly6Chi monocyte differentiation and inflammation in the injured liver. Cell Rep. 2021;37:110026 pubmed publisher
Chen X, MacNabb B, Flood B, Blazar B, Kline J. Divergent fates of antigen-specific CD8+ T cell clones in mice with acute leukemia. Cell Rep. 2021;37:109991 pubmed publisher
Fang F, Cao W, Zhu W, Lam N, Li L, Gaddam S, et al. The cell-surface 5'-nucleotidase CD73 defines a functional T memory cell subset that declines with age. Cell Rep. 2021;37:109981 pubmed publisher
Zong L, Tanaka Yano M, Park B, Yanai H, Turhan F, Croteau D, et al. NAD+ augmentation with nicotinamide riboside improves lymphoid potential of Atm-/- and old mice HSCs. NPJ Aging Mech Dis. 2021;7:25 pubmed publisher
Bates P, Rakhmilevich A, Cho M, Bouchlaka M, Rao S, Hales J, et al. Combining Immunocytokine and Ex Vivo Activated NK Cells as a Platform for Enhancing Graft-Versus-Tumor Effects Against GD2+ Murine Neuroblastoma. Front Immunol. 2021;12:668307 pubmed publisher
Patterson D, Kania A, Price M, Rose J, Scharer C, Boss J. An IRF4-MYC-mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In Vivo. J Immunol. 2021;207:1798-1811 pubmed publisher
Sano T, Kageyama T, Fang V, Kedmi R, Martinez C, Talbot J, et al. Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes. Cell Rep. 2021;36:109608 pubmed publisher
Klarquist J, Cross E, Thompson S, Willett B, Aldridge D, Caffrey Carr A, et al. B cells promote CD8 T cell primary and memory responses to subunit vaccines. Cell Rep. 2021;36:109591 pubmed publisher
Pessoa Rodrigues C, Akhtar A. Differential H4K16ac levels ensure a balance between quiescence and activation in hematopoietic stem cells. Sci Adv. 2021;7: pubmed publisher
Okuniewska M, Fang V, Baeyens A, Raghavan V, Lee J, Littman D, et al. SPNS2 enables T cell egress from lymph nodes during an immune response. Cell Rep. 2021;36:109368 pubmed publisher
Lehmkuhl P, Gentz M, Garcia de Otezya A, Grimbacher B, Schulze Koops H, Skapenko A. Dysregulated immunity in PID patients with low GARP expression on Tregs due to mutations in LRRC32. Cell Mol Immunol. 2021;18:1677-1691 pubmed publisher
Mansouri S, Katikaneni D, Gogoi H, Jin L. Monocyte-Derived Dendritic Cells (moDCs) Differentiate into Bcl6+ Mature moDCs to Promote Cyclic di-GMP Vaccine Adjuvant-Induced Memory TH Cells in the Lung. J Immunol. 2021;206:2233-2245 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Paiva R, Sousa A, Ramos C, Avila M, Lilue J, Paixão T, et al. Self-renewal of double-negative 3 early thymocytes enables thymus autonomy but compromises the β-selection checkpoint. Cell Rep. 2021;35:108967 pubmed publisher
Inoue D, Polaski J, Taylor J, Castel P, Chen S, Kobayashi S, et al. Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Nat Genet. 2021;53:707-718 pubmed publisher
Thomas R, Oh J, Wang W, Su D. Thymic atrophy creates holes in Treg-mediated immuno-regulation via impairment of an antigen-specific clone. Immunology. 2021;163:478-492 pubmed publisher
Bhattacherjee A, Jung J, Zia S, Ho M, Eskandari Sedighi G, St Laurent C, et al. The CD33 short isoform is a gain-of-function variant that enhances Aβ1-42 phagocytosis in microglia. Mol Neurodegener. 2021;16:19 pubmed publisher
Kohn M, Lanfermann C, Laudeley R, Glage S, Rheinheimer C, Klos A. Complement and Chlamydia psittaci: Non-Myeloid-Derived C3 Predominantly Induces Protective Adaptive Immune Responses in Mouse Lung Infection. Front Immunol. 2021;12:626627 pubmed publisher
Gern B, Adams K, Plumlee C, Stoltzfus C, Shehata L, Moguche A, et al. TGFβ restricts expansion, survival, and function of T cells within the tuberculous granuloma. Cell Host Microbe. 2021;29:594-606.e6 pubmed publisher
Frost J, Tan T, Abbas M, Wideman S, Bonadonna M, Stoffel N, et al. Hepcidin-Mediated Hypoferremia Disrupts Immune Responses to Vaccination and Infection. Med. 2021;2:164-179.e12 pubmed publisher
Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
Zhuang Z, Lai X, Sun J, Chen Z, Zhang Z, Dai J, et al. Mapping and role of T cell response in SARS-CoV-2-infected mice. J Exp Med. 2021;218: pubmed publisher
Harsha Krovi S, Zhang J, Michaels Foster M, Brunetti T, Loh L, Scott Browne J, et al. Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells. Nat Commun. 2020;11:6238 pubmed publisher
Li J, Zhang L, Zheng Y, Shao R, Liang Q, Yu W, et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages. elife. 2020;9: pubmed publisher
Alterauge D, Bagnoli J, Dahlström F, Bradford B, Mabbott N, Buch T, et al. Continued Bcl6 Expression Prevents the Transdifferentiation of Established Tfh Cells into Th1 Cells during Acute Viral Infection. Cell Rep. 2020;33:108232 pubmed publisher
Sun Y, Preiss N, Valenteros K, Kamal Y, Usherwood Y, Frost H, et al. Zbtb20 Restrains CD8 T Cell Immunometabolism and Restricts Memory Differentiation and Antitumor Immunity. J Immunol. 2020;205:2649-2666 pubmed publisher
Sandu I, Cerletti D, Claassen M, Oxenius A. Exhausted CD8+ T cells exhibit low and strongly inhibited TCR signaling during chronic LCMV infection. Nat Commun. 2020;11:4454 pubmed publisher
Sadik A, Somarribas Patterson L, Oztürk S, Mohapatra S, Panitz V, Secker P, et al. IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression. Cell. 2020;182:1252-1270.e34 pubmed publisher
Port J, Wozniak D, Oestereich L, Pallasch E, Becker Ziaja B, M xfc ller J, et al. Severe Human Lassa Fever Is Characterized by Nonspecific T-Cell Activation and Lymphocyte Homing to Inflamed Tissues. J Virol. 2020;94: pubmed publisher
Behr F, Beumer Chuwonpad A, Kragten N, Wesselink T, Stark R, van Gisbergen K. Circulating memory CD8+ T cells are limited in forming CD103+ tissue-resident memory T cells at mucosal sites after reinfection. Eur J Immunol. 2021;51:151-166 pubmed publisher
Ramos C, Ballesteros Arias L, Silva J, Paiva R, Nogueira M, Carneiro J, et al. Cell Competition, the Kinetics of Thymopoiesis, and Thymus Cellularity Are Regulated by Double-Negative 2 to 3 Early Thymocytes. Cell Rep. 2020;32:107910 pubmed publisher
Pessoa Rodrigues C, Herman J, Herquel B, Valsecchi C, Stehle T, Grün D, et al. Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate. Sci Adv. 2020;6:eaaz4815 pubmed publisher
Xu P, Tang K, Ma J, Zhang H, Wang D, Zhu L, et al. Chemotherapeutic Tumor Microparticles Elicit a Neutrophil Response Targeting Malignant Pleural Effusions. Cancer Immunol Res. 2020;8:1193-1205 pubmed publisher
Brooks J, Barber J, Davies J, Wells J, Steptoe R. Transfer of antigen-encoding bone marrow under immune-preserving conditions deletes mature antigen-specific B cells in recipients and inhibits antigen-specific antibody production. Cytotherapy. 2020;22:436-444 pubmed publisher
Dourcy M, Maquet C, Dams L, Gilliaux G, Javaux J, Desmecht D, et al. A gammaherpesvirus licenses CD8 T cells to protect the host from pneumovirus-induced immunopathologies. Mucosal Immunol. 2020;13:799-813 pubmed publisher
Hewitson J, West K, James K, Rani G, Dey N, Romano A, et al. Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells. J Immunol. 2020;204:2949-2960 pubmed publisher
García Bonilla M, Ojeda Pérez B, Garcia Martin M, Muñoz Hernández M, Vitorica J, Jimenez S, et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther. 2020;11:121 pubmed publisher
Muri J, Thut H, Feng Q, Kopf M. Thioredoxin-1 distinctly promotes NF-κB target DNA binding and NLRP3 inflammasome activation independently of Txnip. elife. 2020;9: pubmed publisher
Chen C, Sun M, Warzecha C, Bachu M, Dey A, Wu T, et al. HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis. Cell Rep. 2020;30:2136-2149.e4 pubmed publisher
Marchingo J, Sinclair L, Howden A, Cantrell D. Quantitative analysis of how Myc controls T cell proteomes and metabolic pathways during T cell activation. elife. 2020;9: pubmed publisher
Mansouri S, Katikaneni D, Gogoi H, Pipkin M, Machuca T, Emtiazjoo A, et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state. Mucosal Immunol. 2020;13:595-608 pubmed publisher
Pawaria S, Nündel K, Gao K, Moses S, Busto P, Holt K, et al. Role of Interferon-γ-Producing Th1 Cells in a Murine Model of Type I Interferon-Independent Autoinflammation Resulting From DNase II Deficiency. Arthritis Rheumatol. 2020;72:359-370 pubmed publisher
Bhattacharjee S, Mejías Luque R, Loffredo Verde E, Toska A, Flossdorf M, Gerhard M, et al. Concomitant Infection of S. mansoni and H. pylori Promotes Promiscuity of Antigen-Experienced Cells and Primes the Liver for a Lower Fibrotic Response. Cell Rep. 2019;28:231-244.e5 pubmed publisher
Lancaster J, Thyagarajan H, Srinivasan J, Li Y, Hu Z, Ehrlich L. Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance. Nat Commun. 2019;10:2220 pubmed publisher
Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
Wu J, Ma S, Sandhoff R, Ming Y, Hotz Wagenblatt A, Timmerman V, et al. Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 2019;50:1218-1231.e5 pubmed publisher
Sinclair L, Howden A, Brenes A, Spinelli L, Hukelmann J, Macintyre A, et al. Antigen receptor control of methionine metabolism in T cells. elife. 2019;8: pubmed publisher
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van Montfoort N, Borst L, Korrer M, Sluijter M, Marijt K, Santegoets S, et al. NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. Cell. 2018;175:1744-1755.e15 pubmed publisher
Edgar L, Kawasaki N, Nycholat C, Paulson J. Targeted Delivery of Antigen to Activated CD169+ Macrophages Induces Bias for Expansion of CD8+ T Cells. Cell Chem Biol. 2019;26:131-136.e4 pubmed publisher
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Hu Z, Zhang W, Usherwood E. Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect. J Immunol. 2013;191:312-22 pubmed publisher
Ocana Morgner C, Götz A, Wahren C, Jessberger R. SWAP-70 restricts spontaneous maturation of dendritic cells. J Immunol. 2013;190:5545-58 pubmed publisher
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Takada K, Jameson S. Self-class I MHC molecules support survival of naive CD8 T cells, but depress their functional sensitivity through regulation of CD8 expression levels. J Exp Med. 2009;206:2253-69 pubmed publisher
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Pascal V, Nathan N, Claudio E, Siebenlist U, Anderson S. NF-kappa B p50/p65 affects the frequency of Ly49 gene expression by NK cells. J Immunol. 2007;179:1751-9 pubmed
product information
Antigen :
CD45.1
Apps. Abbrev. :
FC
Cat # :
110714
Clone :
A20
Item :
APC anti-mouse CD45.1
Isotype :
Mouse (A.SW) IgG2a, κ
Other Names :
T200, Ly-5.1, LCA
Size :
100 μg
Price (USD) :
127 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
SJL mouse thymocytes and splenocytes
Application Notes :
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^3, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded^7 and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA