product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD45.1
catalog :
110707
quantity :
50 μg
price :
80 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 97
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s3b
Svensson M, Zoccheddu M, Yang S, Nygaard G, Secchi C, Doody K, et al. Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal. Sci Adv. 2020;6:eaba4353 pubmed publisher
  • flow cytometry; mouse; 1:600; loading ...; fig 2s1b, 2s2a
Danzer H, Glaesner J, Baerenwaldt A, Reitinger C, Lux A, Heger L, et al. Human Fcγ-receptor IIb modulates pathogen-specific versus self-reactive antibody responses in lyme arthritis. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 2 ug/ml; loading ...; fig s26a
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a, s1b
Nelson C, Thompson E, Quarnstrom C, Fraser K, Seelig D, Bhela S, et al. Robust Iterative Stimulation with Self-Antigens Overcomes CD8+ T Cell Tolerance to Self- and Tumor Antigens. Cell Rep. 2019;28:3092-3104.e5 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ex4a
Khan O, Giles J, McDonald S, Manne S, Ngiow S, Patel K, et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Halvarsson C, Rörby E, Eliasson P, Lang S, Soneji S, Jönsson J. Putative role of NF-kB but not HIF-1α in hypoxia-dependent regulation of oxidative stress in hematopoietic stem and progenitor cells. Antioxid Redox Signal. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8b
Michaels Y, Barnkob M, Barbosa H, Baeumler T, Thompson M, Andre V, et al. Precise tuning of gene expression levels in mammalian cells. Nat Commun. 2019;10:818 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e8a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s4b
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s7
Barthels C, Ogrinc A, Steyer V, Meier S, Simon F, Wimmer M, et al. CD40-signalling abrogates induction of RORγt+ Treg cells by intestinal CD103+ DCs and causes fatal colitis. Nat Commun. 2017;8:14715 pubmed publisher
  • flow cytometry; mouse; fig 1
Hua J, Stevenson W, Dohlman T, Inomata T, Tahvildari M, Calcagno N, et al. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis. Invest Ophthalmol Vis Sci. 2016;57:1457-67 pubmed publisher
  • flow cytometry; mouse
Chen J, Miyanishi M, Wang S, Yamazaki S, Sinha R, Kao K, et al. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016;530:223-7 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Zhang A, Hostetler A, Chen L, Mukkamala V, Abraham W, Padilla L, et al. Universal redirection of CAR T cells against solid tumours via membrane-inserted ligands for the CAR. Nat Biomed Eng. 2023;7:1113-1128 pubmed publisher
Houlder E, Costain A, Nambuya I, Brown S, Koopman J, Langenberg M, et al. Pulmonary inflammation promoted by type-2 dendritic cells is a feature of human and murine schistosomiasis. Nat Commun. 2023;14:1863 pubmed publisher
Rundberg Nilsson A, Hidalgo I, Bryder D, Pronk C. Temporal dynamics of TNF-mediated changes in hematopoietic stem cell function and recovery. iScience. 2023;26:106341 pubmed publisher
Li X, Li Y, Dong L, Chang Y, Zhang X, Wang C, et al. Decitabine priming increases anti-PD-1 antitumor efficacy by promoting CD8+ progenitor exhausted T cell expansion in tumor models. J Clin Invest. 2023;133: pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
H xe4 ggl xf6 f T, Cipolla M, Loewe M, Chen S, Mesin L, Hartweger H, et al. Continuous germinal center invasion contributes to the diversity of the immune response. Cell. 2023;186:147-161.e15 pubmed publisher
Kozlovski S, Regev O, Sapoznikov A, Kizner M, Achdout H, Petrovich Kopitman E, et al. ICAMs are dispensable for influenza clearance and anti-viral humoral and cellular immunity. Front Immunol. 2022;13:1041552 pubmed publisher
Hodgson R, Xu X, Anzilotti C, Deobagkar Lele M, Crockford T, Kepple J, et al. NDRG1 is induced by antigen-receptor signaling but dispensable for B and T cell self-tolerance. Commun Biol. 2022;5:1216 pubmed publisher
McAlpine C, Kiss M, Zuraikat F, Cheek D, Schiroli G, Amatullah H, et al. Sleep exerts lasting effects on hematopoietic stem cell function and diversity. J Exp Med. 2022;219: pubmed publisher
Miranda K, Becker W, Busbee P, Dopkins N, Abdulla O, Zhong Y, et al. Yin and yang of cannabinoid CB1 receptor: CB1 deletion in immune cells causes exacerbation while deletion in non-immune cells attenuates obesity. iScience. 2022;25:104994 pubmed publisher
Albayrak E, Akgol S, Turan R, Uslu M, Kocabas F. BML-260 promotes the growth of cord blood and mobilized peripheral blood hematopoietic stem and progenitor cells with improved reconstitution ability. J Cell Biochem. 2022;123:2009-2029 pubmed publisher
Takihara Y, Higaki T, Yokomizo T, Umemoto T, Ariyoshi K, Hashimoto M, et al. Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells. Commun Biol. 2022;5:776 pubmed publisher
Waldman M, Rahkola J, Sigler A, Chung J, Willett B, Kedl R, et al. Ena/VASP Protein-Mediated Actin Polymerization Contributes to Naïve CD8+ T Cell Activation and Expansion by Promoting T Cell-APC Interactions In Vivo. Front Immunol. 2022;13:856977 pubmed publisher
Agrawal M, Niroula A, Cunin P, McConkey M, Shkolnik V, Kim P, et al. TET2-mutant clonal hematopoiesis and risk of gout. Blood. 2022;140:1094-1103 pubmed publisher
Ma K, Sun L, Shen M, Zhang X, Xiao Z, Wang J, et al. Functional assessment of the cell-autonomous role of NADase CD38 in regulating CD8+ T cell exhaustion. iScience. 2022;25:104347 pubmed publisher
Morales Mantilla D, Kain B, Le D, Flores A, Paust S, King K. Hematopoietic stem and progenitor cells improve survival from sepsis by boosting immunomodulatory cells. elife. 2022;11: pubmed publisher
De Giovanni M, Tam H, Valet C, Xu Y, Looney M, Cyster J. GPR35 promotes neutrophil recruitment in response to serotonin metabolite 5-HIAA. Cell. 2022;185:815-830.e19 pubmed publisher
Wu L, Xu Y, Zhao H, Zhou Y, Chen Y, Yang S, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12:842-858 pubmed publisher
Aggarwal N, Deerhake M, DiPalma D, Shahi S, Gaggioli M, Mangalam A, et al. Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease. Cell Rep. 2021;37:110170 pubmed publisher
Otano I, Azpilikueta A, Glez Vaz J, Alvarez M, Medina Echeverz J, Cortés Domínguez I, et al. CD137 (4-1BB) costimulation of CD8+ T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulation. Nat Commun. 2021;12:7296 pubmed publisher
Piepke M, Clausen B, Ludewig P, Vienhues J, Bedke T, Javidi E, et al. Interleukin-10 improves stroke outcome by controlling the detrimental Interleukin-17A response. J Neuroinflammation. 2021;18:265 pubmed publisher
Chen X, MacNabb B, Flood B, Blazar B, Kline J. Divergent fates of antigen-specific CD8+ T cell clones in mice with acute leukemia. Cell Rep. 2021;37:109991 pubmed publisher
Patterson D, Kania A, Price M, Rose J, Scharer C, Boss J. An IRF4-MYC-mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In Vivo. J Immunol. 2021;207:1798-1811 pubmed publisher
Sano T, Kageyama T, Fang V, Kedmi R, Martinez C, Talbot J, et al. Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes. Cell Rep. 2021;36:109608 pubmed publisher
Klarquist J, Cross E, Thompson S, Willett B, Aldridge D, Caffrey Carr A, et al. B cells promote CD8 T cell primary and memory responses to subunit vaccines. Cell Rep. 2021;36:109591 pubmed publisher
Bei T, Cao X, Liu Y, Li J, Luo H, Huang L, et al. Antioxidant Fusion Protein SOD1-Tat Increases the Engraftment Efficiency of Total Bone Marrow Cells in Irradiated Mice. Molecules. 2021;26: pubmed publisher
Christian L, Wang L, Lim B, Deng D, Wu H, Wang X, et al. Resident memory T cells in tumor-distant tissues fortify against metastasis formation. Cell Rep. 2021;35:109118 pubmed publisher
Prasad M, Wojciech L, Brzostek J, Hu J, Chua Y, Tung D, et al. Expansion of an Unusual Virtual Memory CD8+ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice. Front Immunol. 2021;12:644483 pubmed publisher
Kohn M, Lanfermann C, Laudeley R, Glage S, Rheinheimer C, Klos A. Complement and Chlamydia psittaci: Non-Myeloid-Derived C3 Predominantly Induces Protective Adaptive Immune Responses in Mouse Lung Infection. Front Immunol. 2021;12:626627 pubmed publisher
Levine L, Hiam Galvez K, Marquez D, Tenvooren I, Madden M, Contreras D, et al. Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8+ T cells during the primary immune response. Immunity. 2021;54:829-844.e5 pubmed publisher
Deerhake M, Danzaki K, Inoue M, Cardakli E, Nonaka T, Aggarwal N, et al. Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M. Immunity. 2021;54:484-498.e8 pubmed publisher
Russler Germain E, Yi J, Young S, Nutsch K, Wong H, Ai T, et al. Gut Helicobacter presentation by multiple dendritic cell subsets enables context-specific regulatory T cell generation. elife. 2021;10: pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Perner C, Flayer C, Zhu X, Aderhold P, Dewan Z, Voisin T, et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020;53:1063-1077.e7 pubmed publisher
Mansell E, Sigurdsson V, Deltcheva E, Brown J, James C, Miharada K, et al. Mitochondrial Potentiation Ameliorates Age-Related Heterogeneity in Hematopoietic Stem Cell Function. Cell Stem Cell. 2021;28:241-256.e6 pubmed publisher
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
Bromley S, Akbaba H, Mani V, Mora Buch R, Chasse A, Sama A, et al. CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell Rep. 2020;32:108085 pubmed publisher
Fumagalli V, Di Lucia P, Venzin V, Bono E, Jordan R, Frey C, et al. Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection. J Exp Med. 2020;217: pubmed publisher
McNamara H, Idris A, Sutton H, Vistein R, Flynn B, Cai Y, et al. Antibody Feedback Limits the Expansion of B Cell Responses to Malaria Vaccination but Drives Diversification of the Humoral Response. Cell Host Microbe. 2020;: pubmed publisher
Winkler E, Shrihari S, Hykes B, Handley S, Andhey P, Huang Y, et al. The Intestinal Microbiome Restricts Alphavirus Infection and Dissemination through a Bile Acid-Type I IFN Signaling Axis. Cell. 2020;182:901-918.e18 pubmed publisher
Kwok I, Becht E, Xia Y, Ng M, Teh Y, Tan L, et al. Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity. 2020;: pubmed publisher
Uslu M, Albayrak E, Kocaba x15f F. Temporal modulation of calcium sensing in hematopoietic stem cells is crucial for proper stem cell expansion and engraftment. J Cell Physiol. 2020;235:9644-9666 pubmed publisher
Koh C, Kim I, Shin K, Jeon I, Song B, Lee J, et al. GITR Agonism Triggers Antitumor Immune Responses through IL21-Expressing Follicular Helper T Cells. Cancer Immunol Res. 2020;8:698-709 pubmed publisher
Derecka M, Herman J, Cauchy P, Ramamoorthy S, Lupar E, Grün D, et al. EBF1-deficient bone marrow stroma elicits persistent changes in HSC potential. Nat Immunol. 2020;21:261-273 pubmed publisher
Hillel Karniel C, Rosen C, Milman Krentsis I, Orgad R, Bachar Lustig E, Shezen E, et al. Multi-lineage Lung Regeneration by Stem Cell Transplantation across Major Genetic Barriers. Cell Rep. 2020;30:807-819.e4 pubmed publisher
Suresh R, Barakat D, Barberi T, Zheng L, Jaffee E, Pienta K, et al. NF-κB p50-deficient immature myeloid cell (p50-IMC) adoptive transfer slows the growth of murine prostate and pancreatic ductal carcinoma. J Immunother Cancer. 2020;8: pubmed publisher
Field C, Baixauli F, Kyle R, Puleston D, Cameron A, Sanin D, et al. Mitochondrial Integrity Regulated by Lipid Metabolism Is a Cell-Intrinsic Checkpoint for Treg Suppressive Function. Cell Metab. 2020;31:422-437.e5 pubmed publisher
Fukushima T, Tanaka Y, Hamey F, Chang C, Oki T, Asada S, et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep. 2019;29:4144-4158.e7 pubmed publisher
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
Ron Harel N, Ghergurovich J, Notarangelo G, LaFleur M, Tsubosaka Y, Sharpe A, et al. T Cell Activation Depends on Extracellular Alanine. Cell Rep. 2019;28:3011-3021.e4 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Miller B, Yates K, Gillis J, et al. PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019;20:1335-1347 pubmed publisher
Delás M, Jackson B, Kovacević T, Vangelisti S, Munera Maravilla E, Wild S, et al. lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation. Cell Rep. 2019;27:719-729.e6 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Uchil P, Pi R, Haugh K, Ladinsky M, Ventura J, Barrett B, et al. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host Microbe. 2019;25:87-100.e10 pubmed publisher
Uchimura T, Oyama Y, Deng M, Guo H, Wilson J, Rampanelli E, et al. The Innate Immune Sensor NLRC3 Acts as a Rheostat that Fine-Tunes T Cell Responses in Infection and Autoimmunity. Immunity. 2018;49:1049-1061.e6 pubmed publisher
Mann M, Mehta A, de Boer C, Kowalczyk M, Lee K, Haldeman P, et al. Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with Age. Cell Rep. 2018;25:2992-3005.e5 pubmed publisher
Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
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product information
Antigen :
CD45.1
Apps. Abbrev. :
FC
Cat # :
110707
Clone :
A20
Item :
PE anti-mouse CD45.1
Isotype :
Mouse (A.SW) IgG2a, κ
Other Names :
T200, Ly-5.1, LCA
Size :
50 μg
Price (USD) :
80 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
SJL mouse thymocytes and splenocytes
Application Notes :
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^3, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded^7 and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA