product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD45.2
catalog :
109815
quantity :
25 μg
price :
94 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
104
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 67
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 4a
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 6a
Wilke J, Hindermann M, Moussavi A, Butt U, Dadarwal R, Berghoff S, et al. Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathol Commun. 2021;9:121 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 2g
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Yang C, Kwon D, Kim M, Im S, Lee Y. Commensal Microbiome Expands Tγδ17 Cells in the Lung and Promotes Particulate Matter-Induced Acute Neutrophilia. Front Immunol. 2021;12:645741 pubmed publisher
  • flow cytometry; mouse; fig 3f
Zhao L, Hu S, Davila M, Yang J, Lin Y, Albanese J, et al. Coordinated co-migration of CCR10+ antibody-producing B cells with helper T cells for colonic homeostatic regulation. Mucosal Immunol. 2021;14:420-430 pubmed publisher
  • flow cytometry; human; 1:200; loading ...
Tullett K, Tan P, Park H, Schittenhelm R, Michael N, Li R, et al. RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 4e
Fernandes R, Li C, Wang G, Yang X, Savvides C, Glassman C, et al. Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b, 3e
Tashita C, Hoshi M, Hirata A, Nakamoto K, Ando T, Hattori T, et al. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice. World J Gastroenterol. 2020;26:918-932 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 8a
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Watanabe M, Kaneko Y, Ohishi Y, Kinoshita M, Sakairi T, Ikeuchi H, et al. Importance of methodology in the evaluation of renal mononuclear phagocytes and analysis of a model of experimental nephritis with Shp1 conditional knockout mice. Biochem Biophys Rep. 2020;22:100741 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Kumar A, Chamoto K, Chowdhury P, Honjo T. Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6, 5g
Sun D, Zhang M, Sun P, Liu G, Strickland A, Chen Y, et al. VCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection. PLoS Pathog. 2020;16:e1008361 pubmed publisher
  • flow cytometry; mouse; 2.5 ug/ml; loading ...; fig s26a
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s9b
Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2j
Mesin L, Schiepers A, Ersching J, Barbulescu A, Cavazzoni C, Angelini A, et al. Restricted Clonality and Limited Germinal Center Reentry Characterize Memory B Cell Reactivation by Boosting. Cell. 2020;180:92-106.e11 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19b
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Schreiber L, Urbiola C, Das K, Spiesschaert B, Kimpel J, Heinemann F, et al. The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer. Br J Cancer. 2019;121:647-658 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6a
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; 1:600; loading ...; fig 5e
Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig ex4a
Khan O, Giles J, McDonald S, Manne S, Ngiow S, Patel K, et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8a
Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, et al. Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5-/- mice. Nat Commun. 2019;10:1492 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s10a
Lee J, Stone M, Porrett P, Thomas S, Komar C, Li J, et al. Hepatocytes direct the formation of a pro-metastatic niche in the liver. Nature. 2019;567:249-252 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Martins J, Andoniou C, Fleming P, Kuns R, Schuster I, Voigt V, et al. Strain-specific antibody therapy prevents cytomegalovirus reactivation after transplantation. Science. 2019;363:288-293 pubmed publisher
  • flow cytometry; mouse; fig 2a
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mahr B, Pilat N, Granofszky N, Wiletel M, Muckenhuber M, Maschke S, et al. Hybrid resistance to parental bone marrow grafts in nonlethally irradiated mice. Am J Transplant. 2019;19:591-596 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19a
Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Lund H, Pieber M, Parsa R, Han J, Grommisch D, Ewing E, et al. Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells. Nat Commun. 2018;9:4845 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1c
He Z, Zhang J, Huang Z, Du Q, Li N, Zhang Q, et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun. 2018;9:4870 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Paschalis E, Lei F, Zhou C, Kapoulea V, Dana R, Chodosh J, et al. Permanent neuroglial remodeling of the retina following infiltration of CSF1R inhibition-resistant peripheral monocytes. Proc Natl Acad Sci U S A. 2018;115:E11359-E11368 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4g
Meyer I, Goetzke C, Kespohl M, Sauter M, Heuser A, Eckstein V, et al. Silencing the CSF-1 Axis Using Nanoparticle Encapsulated siRNA Mitigates Viral and Autoimmune Myocarditis. Front Immunol. 2018;9:2303 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Singh M, Ni M, Sullivan J, Hamerman J, Campbell D. B cell adaptor for PI3-kinase (BCAP) modulates CD8+ effector and memory T cell differentiation. J Exp Med. 2018;215:2429-2443 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e8a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Shaw T, Houston S, Wemyss K, Bridgeman H, Barbera T, Zangerle Murray T, et al. Tissue-resident macrophages in the intestine are long lived and defined by Tim-4 and CD4 expression. J Exp Med. 2018;215:1507-1518 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3
Hsiao H, Fernandez R, Tanaka S, Li W, Spahn J, Chiu S, et al. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1β. J Clin Invest. 2018;128:2833-2847 pubmed publisher
  • flow cytometry; mouse; fig 4f
Yang K, Liang Y, Sun Z, Xue D, Xu H, Zhu M, et al. T Cell-Derived Lymphotoxin Is Essential for the Anti-Herpes Simplex Virus 1 Humoral Immune Response. J Virol. 2018;92: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3c
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; human; 1:100; fig 2c
Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
  • flow cytometry; mouse; fig 5c
Capucha T, Koren N, Nassar M, Heyman O, Nir T, Levy M, et al. Sequential BMP7/TGF-β1 signaling and microbiota instruct mucosal Langerhans cell differentiation. J Exp Med. 2018;215:481-500 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4a
Vo L, Kinney M, Liu X, Zhang Y, Barragan J, Sousa P, et al. Regulation of embryonic haematopoietic multipotency by EZH1. Nature. 2018;553:506-510 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4f
Clarke A, Riffelmacher T, Braas D, Cornall R, Simon A. B1a B cells require autophagy for metabolic homeostasis and self-renewal. J Exp Med. 2018;215:399-413 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2d
Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
  • flow cytometry; mouse; fig 3
Hornick E, Banoth B, Miller A, Zacharias Z, Jain N, Wilson M, et al. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection. J Immunol. 2018;200:1188-1197 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Zhang S, Takaku M, Zou L, Gu A, Chou W, Zhang G, et al. Reversing SKI-SMAD4-mediated suppression is essential for TH17 cell differentiation. Nature. 2017;551:105-109 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1b
Roufaiel M, Gracey E, Siu A, Zhu S, Lau A, Ibrahim H, et al. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima. Nat Immunol. 2016;17:1263-1272 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1h
Schneider C, Nobs S, Heer A, Hirsch E, Penninger J, Siggs O, et al. Frontline Science: Coincidental null mutation of Csf2rα in a colony of PI3Kγ-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection. J Leukoc Biol. 2017;101:367-376 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6
Damle S, Martin R, Cross J, Conrad D. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol. 2017;10:205-214 pubmed publisher
Inverso D, Shi J, Lee K, Jakab M, Ben Moshe S, Kulkarni S, et al. A spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie-Wnt signaling axis in the liver. Dev Cell. 2021;56:1677-1693.e10 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Wang X, Ray R, Kratochvil S, Melzi E, Lin Y, Giguere S, et al. Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors. EMBO J. 2021;40:e105926 pubmed publisher
Hirai T, Yang Y, Zenke Y, Li H, Chaudhri V, De La Cruz Diaz J, et al. Competition for Active TGFβ Cytokine Allows for Selective Retention of Antigen-Specific Tissue- Resident Memory T Cells in the Epidermal Niche. Immunity. 2021;54:84-98.e5 pubmed publisher
Akkaya M, Bansal A, Sheehan P, Pena M, Cimperman C, Qi C, et al. Testing the impact of a single nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor on experimental cerebral malaria in mice. Sci Rep. 2020;10:13630 pubmed publisher
Marchingo J, Sinclair L, Howden A, Cantrell D. Quantitative analysis of how Myc controls T cell proteomes and metabolic pathways during T cell activation. elife. 2020;9: pubmed publisher
Wang L, Li Y, Wang X, Wang P, Essandoh K, Cui S, et al. GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype. Cells. 2020;9: pubmed publisher
Zhang F, Zhao Q, Jiang Y, Liu N, Liu Q, Shi F, et al. Augmented Brain Infiltration and Activation of Leukocytes After Cerebral Ischemia in Type 2 Diabetic Mice. Front Immunol. 2019;10:2392 pubmed publisher
Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
Sinclair L, Howden A, Brenes A, Spinelli L, Hukelmann J, Macintyre A, et al. Antigen receptor control of methionine metabolism in T cells. elife. 2019;8: pubmed publisher
Peterson E, Bailo R, Rothchild A, Arrieta Ortiz M, Kaur A, Pan M, et al. Path-seq identifies an essential mycolate remodeling program for mycobacterial host adaptation. Mol Syst Biol. 2019;15:e8584 pubmed publisher
Lever J, Hull T, Boddu R, Pepin M, Black L, Adedoyin O, et al. Resident macrophages reprogram toward a developmental state after acute kidney injury. JCI Insight. 2019;4: pubmed publisher
Li J, Lee Y, Li Y, Jiang Y, Lu H, Zang W, et al. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells. Immunity. 2018;48:773-786.e5 pubmed publisher
Jonas B, Johnson C, Gratzinger D, Majeti R. Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity. PLoS ONE. 2016;11:e0159189 pubmed publisher
Hirasawa M, Takubo K, Osada H, Miyake S, Toda E, Endo M, et al. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration. J Biol Chem. 2016;291:7373-85 pubmed publisher
Wen T, Besse J, Mingler M, Fulkerson P, Rothenberg M. Eosinophil adoptive transfer system to directly evaluate pulmonary eosinophil trafficking in vivo. Proc Natl Acad Sci U S A. 2013;110:6067-72 pubmed publisher
Nakano Yokomizo T, Tahara Hanaoka S, Nakahashi Oda C, Nabekura T, Tchao N, Kadosaki M, et al. The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses. J Exp Med. 2011;208:1661-71 pubmed publisher
product information
Antigen :
CD45.2
Apps. Abbrev. :
Flow Cytometry
Cat # :
109815
Clone :
104
Item :
Alexa Fluor® 488 anti-mouse CD45.2
Isotype :
Mouse (SJL) IgG2a, κ
Other Names :
Ly-5.2, LCA
Size :
25 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Immunogen :
B10.S mouse thymocytes and splenocytes
Application Notes :
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation 4 , in vivo and in vitro blocking of B cell responses 1,2 , and immunohistochemical staining of acetone-fixed frozen sections 3 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA