product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse NK-1.1
catalog :
108761
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PK136
reactivity :
mouse
application :
neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 99
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4b
Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a, s2
Aiken T, Erbe A, Zebertavage L, Komjathy D, Feils A, Rodriguez M, et al. Mechanism of effective combination radio-immunotherapy against 9464D-GD2, an immunologically cold murine neuroblastoma. J Immunother Cancer. 2022;10: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2f
Bartsch P, Kilian C, Hellmig M, Paust H, Borchers A, Sivayoganathan A, et al. Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection. PLoS Pathog. 2022;18:e1010430 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
  • flow cytometry; mouse; fig s4c
Wang H, Wang Q, Yang C, Guo M, Cui X, Jing Z, et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut Microbes. 2022;14:2027853 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b, 4f
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:700; loading ...; fig 2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...
Kong W, Tsuyama N, Inoue H, Guo Y, Mokuda S, Nobukiyo A, et al. Long-chain saturated fatty acids in breast milk are associated with the pathogenesis of atopic dermatitis via induction of inflammatory ILC3s. Sci Rep. 2021;11:13109 pubmed publisher
  • flow cytometry; mouse; fig 6c
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s7b
Ercolano G, Gomez Cadena A, Dumauthioz N, Vanoni G, Kreutzfeldt M, Wyss T, et al. PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions. Nat Commun. 2021;12:2538 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Gao L, Li B, Wang J, Shen D, Yang M, Sun R, et al. Activation of Liver X Receptor α Sensitizes Mice to T-Cell Mediated Hepatitis. Hepatol Commun. 2020;4:1664-1679 pubmed publisher
  • flow cytometry; mouse; loading ...
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Shi H, Lo T, Ma D, Condor B, Lesmana B, Parungao R, et al. Dihydrotestosterone (DHT) Enhances Wound Healing of Major Burn Injury by Accelerating Resolution of Inflammation in Mice. Int J Mol Sci. 2020;21: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s6-1b
Ricci B, Tycksen E, Celik H, Belle J, Fontana F, Civitelli R, et al. Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2d
Li N, Kang Y, Wang L, Huff S, Tang R, Hui H, et al. ALKBH5 regulates anti-PD-1 therapy response by modulating lactate and suppressive immune cell accumulation in tumor microenvironment. Proc Natl Acad Sci U S A. 2020;117:20159-20170 pubmed publisher
  • flow cytometry; mouse; 1:800; loading ...; fig 2s1b
Burfeind K, Zhu X, Norgard M, Levasseur P, Huisman C, Buenafe A, et al. Circulating myeloid cells invade the central nervous system to mediate cachexia during pancreatic cancer. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 1a, 1d
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • blocking or activating experiments; mouse; ; fig 2f
  • flow cytometry; mouse; loading ...; fig 2g
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3, 1g
Sun D, Zhang M, Sun P, Liu G, Strickland A, Chen Y, et al. VCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection. PLoS Pathog. 2020;16:e1008361 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Clark D, Brazina S, Yang F, Hu D, Hsieh C, Niemi E, et al. Age-related changes to macrophages are detrimental to fracture healing in mice. Aging Cell. 2020;19:e13112 pubmed publisher
  • flow cytometry; mouse; 2 ug/ml; loading ...; fig s3a
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Schanoski A, Le T, Kaiserman D, Rowe C, Prow N, Barboza D, et al. Granzyme A in Chikungunya and Other Arboviral Infections. Front Immunol. 2019;10:3083 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16d
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Cheng M, Chen Y, Huang D, Chen W, Xu W, Chen Y, et al. Intrinsically altered lung-resident γδT cells control lung melanoma by producing interleukin-17A in the elderly. Aging Cell. 2020;19:e13099 pubmed publisher
  • flow cytometry; mouse; fig 2f
Williford J, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell T, et al. Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy. Sci Adv. 2019;5:eaay1357 pubmed publisher
  • mass cytometry; mouse; 6 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; 1:33; loading ...; fig 3d
Fukuda Y, Asaoka T, Eguchi H, Yokota Y, Kubo M, Kinoshita M, et al. Endogenous CXCL9 affects prognosis by regulating tumor-infiltrating natural killer cells in intrahepatic cholangiocarcinoma. Cancer Sci. 2020;111:323-333 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19c
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6a
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
  • flow cytometry; mouse; fig 2b
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Mahr B, Pilat N, Granofszky N, Wiletel M, Muckenhuber M, Maschke S, et al. Hybrid resistance to parental bone marrow grafts in nonlethally irradiated mice. Am J Transplant. 2019;19:591-596 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Qu J, Li L, Xie H, Zhang X, Yang Q, Qiu H, et al. TLR3 Modulates the Response of NK Cells against Schistosoma japonicum. J Immunol Res. 2018;2018:7519856 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
Yu Y, Wu X, Wang M, Liu W, Zhang L, Zhang Y, et al. Optogenetic-controlled immunotherapeutic designer cells for post-surgical cancer immunotherapy. Nat Commun. 2022;13:6357 pubmed publisher
Hu J, He J, Wang Y, Zhao Y, Fang K, Dong Y, et al. Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy. J Immunother Cancer. 2022;10: pubmed publisher
Wang F, Zhang X, Liu W, Zhou Y, Wei W, Liu D, et al. Activated Natural Killer Cell Promotes Nonalcoholic Steatohepatitis Through Mediating JAK/STAT Pathway. Cell Mol Gastroenterol Hepatol. 2022;13:257-274 pubmed publisher
Sheppard S, Santosa E, Lau C, Violante S, Giovanelli P, Kim H, et al. Lactate dehydrogenase A-dependent aerobic glycolysis promotes natural killer cell anti-viral and anti-tumor function. Cell Rep. 2021;35:109210 pubmed publisher
Murdock B, Famie J, Piecuch C, Raue K, Mendelson F, Pieroni C, et al. NK cells associate with ALS in a sex- and age-dependent manner. JCI Insight. 2021;6: pubmed publisher
Shreeve N, Depierreux D, Hawkes D, Traherne J, Sovio U, Huhn O, et al. The CD94/NKG2A inhibitory receptor educates uterine NK cells to optimize pregnancy outcomes in humans and mice. Immunity. 2021;54:1231-1244.e4 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
Pham T, Meziane O, Miah M, Volodina O, Colas C, Beland K, et al. Flt3L-Mediated Expansion of Plasmacytoid Dendritic Cells Suppresses HIV Infection in Humanized Mice. Cell Rep. 2019;29:2770-2782.e5 pubmed publisher
Petermann F, Pekowska A, Johnson C, Jankovic D, Shih H, Jiang K, et al. The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1. Mol Cell. 2019;75:1229-1242.e5 pubmed publisher
Li J, Wada S, Weaver L, Biswas C, Behrens E, Arany Z. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis. JCI Insight. 2019;5: pubmed publisher
Haikala H, Anttila J, Marques E, Raatikainen T, Ilander M, Hakanen H, et al. Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. Nat Commun. 2019;10:620 pubmed publisher
Davies A, Kim H, González Cano R, Choi J, Back S, Roh S, et al. Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury. Cell. 2019;176:716-728.e18 pubmed publisher
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
van Montfoort N, Borst L, Korrer M, Sluijter M, Marijt K, Santegoets S, et al. NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. Cell. 2018;175:1744-1755.e15 pubmed publisher
Sandrock I, Reinhardt A, Ravens S, Binz C, Wilharm A, Martins J, et al. Genetic models reveal origin, persistence and non-redundant functions of IL-17-producing γδ T cells. J Exp Med. 2018;215:3006-3018 pubmed publisher
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Gunda V, Gigliotti B, Ndishabandi D, Ashry T, McCarthy M, Zhou Z, et al. Combinations of BRAF inhibitor and anti-PD-1/PD-L1 antibody improve survival and tumour immunity in an immunocompetent model of orthotopic murine anaplastic thyroid cancer. Br J Cancer. 2018;119:1223-1232 pubmed publisher
Du J, Ding X, Zhang X, Zhao X, Shan H, Wang F. Berberine attenuate staphylococcal enterotoxin B-mediated acute liver injury via regulating HDAC expression. AMB Express. 2018;8:158 pubmed publisher
Giambra V, Gusscott S, Gracias D, Song R, Lam S, Panelli P, et al. Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity. Cell Stem Cell. 2018;23:714-726.e7 pubmed publisher
Lee S, North K, Kim E, Jang E, Obeng E, Lu S, et al. Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations. Cancer Cell. 2018;34:225-241.e8 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
Bin N, Ma K, Tien C, Wang S, Zhu D, Park S, et al. C2 Domains of Munc13-4 Are Crucial for Ca2+-Dependent Degranulation and Cytotoxicity in NK Cells. J Immunol. 2018;201:700-713 pubmed publisher
Wein A, Dunbar P, McMaster S, Li Z, Denning T, Kohlmeier J. IL-36γ Protects against Severe Influenza Infection by Promoting Lung Alveolar Macrophage Survival and Limiting Viral Replication. J Immunol. 2018;201:573-582 pubmed publisher
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
Crosby E, Wei J, Yang X, Lei G, Wang T, Liu C, et al. Complimentary mechanisms of dual checkpoint blockade expand unique T-cell repertoires and activate adaptive anti-tumor immunity in triple-negative breast tumors. Oncoimmunology. 2018;7:e1421891 pubmed publisher
Sui P, Wiesner D, Xu J, Zhang Y, Lee J, Van Dyken S, et al. Pulmonary neuroendocrine cells amplify allergic asthma responses. Science. 2018;360: pubmed publisher
Panduro M, Benoist C, Mathis D. Treg cells limit IFN-? production to control macrophage accrual and phenotype during skeletal muscle regeneration. Proc Natl Acad Sci U S A. 2018;115:E2585-E2593 pubmed publisher
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Orta Mascaró M, Consuegra Fernández M, Carreras E, Roncagalli R, Carreras Sureda A, Alvarez P, et al. CD6 modulates thymocyte selection and peripheral T cell homeostasis. J Exp Med. 2016;213:1387-97 pubmed publisher
product information
Antigen :
NK-1.1
Apps. Abbrev. :
FC, CyTOF®, IP, Depletion, Block, IHC-F
Cat # :
108761
Clone :
PK136
Item :
Ultra-LEAF™ Purified anti-mouse NK-1.1
Isotype :
Mouse IgG2a, κ
Other Names :
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Immunogen :
NK-1 + cells from mouse spleen and bone marrow
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity^3, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis^6, blocking of NK cell function^7, induction of proliferation^8, immunohistochemical staining of frozen sections^11, immunofluorescence microscopy^11, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA