This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-mouse NK-1.1
catalog :
108712
quantity :
500 μg
price :
145 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PK136
reactivity :
human, mouse
application :
immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
citations: 101
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; loading ...; fig 5h
Davies A, Kim H, González Cano R, Choi J, Back S, Roh S, et al. Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury. Cell. 2019;176:716-728.e18 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Martins J, Andoniou C, Fleming P, Kuns R, Schuster I, Voigt V, et al. Strain-specific antibody therapy prevents cytomegalovirus reactivation after transplantation. Science. 2019;363:288-293 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5e
Ruscetti M, Leibold J, Bott M, Fennell M, Kulick A, Salgado N, et al. NK cell-mediated cytotoxicity contributes to tumor control by a cytostatic drug combination. Science. 2018;362:1416-1422 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4b
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Xiao F, Ai G, Yan W, Wan X, Luo X, Ning Q. Intrahepatic recruitment of cytotoxic NK cells contributes to autoimmune hepatitis progression. Cell Immunol. 2018;327:13-20 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2b
Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s4c
Campana L, Starkey Lewis P, Pellicoro A, Aucott R, Man J, O Duibhir E, et al. The STAT3-IL-10-IL-6 Pathway Is a Novel Regulator of Macrophage Efferocytosis and Phenotypic Conversion in Sterile Liver Injury. J Immunol. 2018;200:1169-1187 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Kwan B, Zhu E, Tzeng A, Sugito H, Eltahir A, Ma B, et al. Integrin-targeted cancer immunotherapy elicits protective adaptive immune responses. J Exp Med. 2017;214:1679-1690 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Kogo H, Shimizu M, Negishi Y, Uchida E, Takahashi H. Suppression of murine tumour growth through CD8+ cytotoxic T lymphocytes via activated DEC-205+ dendritic cells by sequential administration of ?-galactosylceramide in vivo. Immunology. 2017;151:324-339 pubmed publisher
  • flow cytometry; mouse; fig 1b
Gardner P, Liyanage S, Cristante E, Sampson R, Dick A, Ali R, et al. Hypoxia inducible factors are dispensable for myeloid cell migration into the inflamed mouse eye. Sci Rep. 2017;7:40830 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Rombouts M, Cools N, Grootaert M, de Bakker F, Van Brussel I, Wouters A, et al. Long-Term Depletion of Conventional Dendritic Cells Cannot Be Maintained in an Atherosclerotic Zbtb46-DTR Mouse Model. PLoS ONE. 2017;12:e0169608 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8d
van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s2a
von Moltke J, O Leary C, Barrett N, Kanaoka Y, Austen K, Locksley R. Leukotrienes provide an NFAT-dependent signal that synergizes with IL-33 to activate ILC2s. J Exp Med. 2017;214:27-37 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; fig s1
Kong S, Yang Y, Xu Y, Wang Y, Zhang Y, Melo Cardenas J, et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A. 2016;113:10394-9 pubmed publisher
  • blocking or activating experiments; human; fig 5
  • flow cytometry; human; fig 5
Di Scala M, Otano I, Gil Farina I, Vanrell L, Hommel M, Olague C, et al. Complementary Effects of Interleukin-15 and Alpha Interferon Induce Immunity in Hepatitis B Virus Transgenic Mice. J Virol. 2016;90:8563-74 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Lo T, Silveira P, Fromm P, Verma N, Vu P, Kupresanin F, et al. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration. J Immunol. 2016;197:885-98 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Qualai J, Li L, Cantero J, Tarrats A, Fernández M, Sumoy L, et al. Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. PLoS ONE. 2016;11:e0154253 pubmed publisher
  • flow cytometry; mouse; fig s2
Li Z, Hodgkinson T, Gothard E, Boroumand S, Lamb R, Cummins I, et al. Epidermal Notch1 recruits RORγ(+) group 3 innate lymphoid cells to orchestrate normal skin repair. Nat Commun. 2016;7:11394 pubmed publisher
  • flow cytometry; mouse
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; mouse; fig 1
Pelly V, Kannan Y, Coomes S, Entwistle L, Rückerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
  • flow cytometry; mouse
Kim K, Kim N, Lee G. Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region. PLoS ONE. 2016;11:e0148576 pubmed publisher
  • flow cytometry; mouse
Brasseit J, Althaus Steiner E, Faderl M, Dickgreber N, Saurer L, Genitsch V, et al. CD4 T cells are required for both development and maintenance of disease in a new mouse model of reversible colitis. Mucosal Immunol. 2016;9:689-701 pubmed publisher
  • flow cytometry; mouse; fig s7
McCormack R, de Armas L, Shiratsuchi M, Fiorentino D, Olsson M, Lichtenheld M, et al. Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. elife. 2015;4: pubmed publisher
  • flow cytometry; mouse
Dahlgren M, Gustafsson Hedberg T, Livingston M, Cucak H, Alsén S, Yrlid U, et al. T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells. J Immunol. 2015;194:5187-99 pubmed publisher
  • flow cytometry; mouse; fig s2
Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
  • flow cytometry; mouse
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • flow cytometry; mouse
Peters A, Burkett P, Sobel R, Buckley C, Watson S, Bettelli E, et al. Podoplanin negatively regulates CD4+ effector T cell responses. J Clin Invest. 2015;125:129-40 pubmed publisher
  • flow cytometry; mouse
Guttman O, Yossef R, Freixo Lima G, Rider P, Porgador A, Lewis E. α1-Antitrypsin modifies general NK cell interactions with dendritic cells and specific interactions with islet β-cells in favor of protection from autoimmune diabetes. Immunology. 2014;: pubmed publisher
  • flow cytometry; mouse
Chen J, Zhao Y, Zhang C, Chen H, Feng J, Chi X, et al. Persistent hepatitis C virus infections and hepatopathological manifestations in immune-competent humanized mice. Cell Res. 2014;24:1050-66 pubmed publisher
  • flow cytometry; mouse
Madireddi S, Eun S, Lee S, Nemčovičová I, Mehta A, Zajonc D, et al. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med. 2014;211:1433-48 pubmed publisher
  • flow cytometry; mouse
Wickström S, Oberg L, Kärre K, Johansson M. A genetic defect in mice that impairs missing self recognition despite evidence for normal maturation and MHC class I-dependent education of NK cells. J Immunol. 2014;192:1577-86 pubmed publisher
Li J, Wada S, Weaver L, Biswas C, Behrens E, Arany Z. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis. JCI Insight. 2019;5: pubmed publisher
Simonović N, Witalisz Siepracka A, Meissl K, Lassnig C, Reichart U, Kolbe T, et al. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity. J Immunol. 2019;202:1724-1734 pubmed publisher
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
Kiyohara H, Sujino T, Teratani T, Miyamoto K, Arai M, Nomura E, et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol. 2019;7:135-156 pubmed publisher
Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
Sandrock I, Reinhardt A, Ravens S, Binz C, Wilharm A, Martins J, et al. Genetic models reveal origin, persistence and non-redundant functions of IL-17-producing γδ T cells. J Exp Med. 2018;215:3006-3018 pubmed publisher
Barikbin R, Berkhout L, Bolik J, Schmidt Arras D, Ernst T, Ittrich H, et al. Early heme oxygenase 1 induction delays tumour initiation and enhances DNA damage repair in liver macrophages of Mdr2-/- mice. Sci Rep. 2018;8:16238 pubmed publisher
Kim G, Jo Y, Lee B, Ali L, Lee B, Hong C. The Potential Role of a Soluble γ-Chain Cytokine Receptor as a Regulator of IL-7-Induced Lymphoproliferative Disorders. Int J Mol Sci. 2018;19: pubmed publisher
Disson O, Blériot C, Jacob J, Serafini N, Dulauroy S, Jouvion G, et al. Peyer's patch myeloid cells infection by Listeria signals through gp38+ stromal cells and locks intestinal villus invasion. J Exp Med. 2018;215:2936-2954 pubmed publisher
Almeida F, Tognarelli S, Marcais A, Kueh A, Friede M, Liao Y, et al. A point mutation in the Ncr1 signal peptide impairs the development of innate lymphoid cell subsets. Oncoimmunology. 2018;7:e1475875 pubmed publisher
Qu J, Li L, Xie H, Zhang X, Yang Q, Qiu H, et al. TLR3 Modulates the Response of NK Cells against Schistosoma japonicum. J Immunol Res. 2018;2018:7519856 pubmed publisher
Hansell C, Fraser A, Hayes A, Pingen M, Burt C, Lee K, et al. The Atypical Chemokine Receptor Ackr2 Constrains NK Cell Migratory Activity and Promotes Metastasis. J Immunol. 2018;201:2510-2519 pubmed publisher
Breuer J, Korpos E, Hannocks M, Schneider Hohendorf T, Song J, Zondler L, et al. Blockade of MCAM/CD146 impedes CNS infiltration of T cells over the choroid plexus. J Neuroinflammation. 2018;15:236 pubmed publisher
Lee S, North K, Kim E, Jang E, Obeng E, Lu S, et al. Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations. Cancer Cell. 2018;34:225-241.e8 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Jensen C, Åhsberg J, Sommarin M, Strid T, Somasundaram R, Okuyama K, et al. Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis. J Exp Med. 2018;215:1947-1963 pubmed publisher
Wei B, Zhang R, Zhai J, Zhu J, Yang F, Yue D, et al. Suppression of Th17 Cell Response in the Alleviation of Dextran Sulfate Sodium-Induced Colitis by Ganoderma lucidum Polysaccharides. J Immunol Res. 2018;2018:2906494 pubmed publisher
Wein A, Dunbar P, McMaster S, Li Z, Denning T, Kohlmeier J. IL-36γ Protects against Severe Influenza Infection by Promoting Lung Alveolar Macrophage Survival and Limiting Viral Replication. J Immunol. 2018;201:573-582 pubmed publisher
Kyung D, Sung H, Kim Y, Kim K, Cho S, Choi J, et al. Global transcriptome analysis identifies weight regain-induced activation of adaptive immune responses in white adipose tissue of mice. Int J Obes (Lond). 2018;42:755-764 pubmed publisher
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
Crosby E, Wei J, Yang X, Lei G, Wang T, Liu C, et al. Complimentary mechanisms of dual checkpoint blockade expand unique T-cell repertoires and activate adaptive anti-tumor immunity in triple-negative breast tumors. Oncoimmunology. 2018;7:e1421891 pubmed publisher
Gounder A, Yokoyama C, Jarjour N, Bricker T, Edelson B, Boon A. Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer. PLoS Pathog. 2018;14:e1007001 pubmed publisher
Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell. 2018;173:634-648.e12 pubmed publisher
Sui P, Wiesner D, Xu J, Zhang Y, Lee J, Van Dyken S, et al. Pulmonary neuroendocrine cells amplify allergic asthma responses. Science. 2018;360: pubmed publisher
Li Q, Dai C, Xue R, Wang P, Chen L, Han Y, et al. S100A4 Protects Myeloid-Derived Suppressor Cells from Intrinsic Apoptosis via TLR4-ERK1/2 Signaling. Front Immunol. 2018;9:388 pubmed publisher
Panduro M, Benoist C, Mathis D. Treg cells limit IFN-? production to control macrophage accrual and phenotype during skeletal muscle regeneration. Proc Natl Acad Sci U S A. 2018;115:E2585-E2593 pubmed publisher
Ordoñez C, Savage H, Tarajia M, Rivera R, Weeks Galindo C, Sambrano D, et al. Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells. Immunology. 2018;: pubmed publisher
Cervantes Luevano K, Caronni N, Castiello M, Fontana E, Piperno G, Naseem A, et al. Neutrophils drive type I interferon production and autoantibodies in patients with Wiskott-Aldrich syndrome. J Allergy Clin Immunol. 2018;142:1605-1617.e4 pubmed publisher
Kim D, Park H, Lim S, Koo J, Lee H, Choi J, et al. Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis. Nat Commun. 2018;9:503 pubmed publisher
McKenney A, Lau A, Somasundara A, Spitzer B, Intlekofer A, Ahn J, et al. JAK2/IDH-mutant-driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. J Clin Invest. 2018;128:789-804 pubmed publisher
Renand A, Shamji M, Harris K, Qin T, Wambre E, Scadding G, et al. Synchronous immune alterations mirror clinical response during allergen immunotherapy. J Allergy Clin Immunol. 2018;141:1750-1760.e1 pubmed publisher
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Albertí Servera L, von Muenchow L, Tsapogas P, Capoferri G, Eschbach K, Beisel C, et al. Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors. EMBO J. 2017;36:3619-3633 pubmed publisher
Glasner A, Isaacson B, Viukov S, Neuman T, Friedman N, Mandelboim M, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression. Sci Rep. 2017;7:13090 pubmed publisher
Kim H, Jang S, Lee W, Kim K, Sohn H, Hwang S, et al. PTEN drives Th17 cell differentiation by preventing IL-2 production. J Exp Med. 2017;214:3381-3398 pubmed publisher
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Hosomi S, Grootjans J, Tschurtschenthaler M, Krupka N, Matute J, Flak M, et al. Intestinal epithelial cell endoplasmic reticulum stress promotes MULT1 up-regulation and NKG2D-mediated inflammation. J Exp Med. 2017;214:2985-2997 pubmed publisher
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product information
Antigen :
NK-1.1
Apps. Abbrev. :
Flow Cytometry, CyTOF®, IP, Deplete, Block, IHC, Immunofluorescence Microscopy
Cat # :
108712
Clone :
PK136
Item :
LEAF™ Purified anti-mouse NK-1.1
Isotype :
Mouse IgG2a, κ
Other Names :
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Size :
500 μg
Price (USD) :
145 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
LEAF
Immunogen :
NK-1 + cells from mouse spleen and bone marrow
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , complement-dependent cytotoxicity 3 , in vivo depletion 4,5,9,10 , mediation of in vitro redirected lysis 6 , blocking of NK cell function 7 , induction of proliferation 8 , immunohistochemical staining of frozen sections 11 , and immunofluorescence microscopy 11 . The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 108712).
company information
BioLegend
9727 Pacific Heights Blvd.
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA