product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108461
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
RB6-8C5
reactivity :
mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, other
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 3
Kumagai Y, Futoh Y, Miyato H, Ohzawa H, Yamaguchi H, Saito S, et al. Effect of Systemic or Intraperitoneal Administration of Anti-PD-1 Antibody for Peritoneal Metastases from Gastric Cancer. In Vivo. 2022;36:1126-1135 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s2f
Bartsch P, Kilian C, Hellmig M, Paust H, Borchers A, Sivayoganathan A, et al. Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection. PLoS Pathog. 2022;18:e1010430 pubmed publisher
  • flow cytometry; mouse; fig s4a
Wang H, Wang Q, Yang C, Guo M, Cui X, Jing Z, et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut Microbes. 2022;14:2027853 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig 2d
Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:1500; loading ...; fig s2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Gurley J, Gmyrek G, Hargis E, Bishop G, Carr D, Elliott M. The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation. Cells. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 7c
Grandits A, Nguyen C, Schlerka A, Hackl H, Sill H, Etzler J, et al. Downregulation of MTSS1 in acute myeloid leukemia is associated with a poor prognosis, chemotherapy resistance, and disease aggressiveness. Leukemia. 2021;35:2827-2839 pubmed publisher
  • other; mouse; fig 4a
  • flow cytometry; mouse; loading ...; fig 1a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d
Chen J, Cao X, Li B, Zhao Z, Chen S, Lai S, et al. Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Front Immunol. 2020;11:621757 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig e8c, e8d
Mastorakos P, Mihelson N, Luby M, Burks S, Johnson K, Hsia A, et al. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021;24:245-258 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Gurley J, Gmyrek G, McClellan M, Hargis E, Hauck S, Dozmorov M, et al. Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina. Invest Ophthalmol Vis Sci. 2020;61:19 pubmed publisher
Zhu S, Zhang J, Xue N, Zhu X, Li F, Dai Q, et al. Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. J Nanobiotechnology. 2023;21:81 pubmed publisher
Frost J, Wideman S, Preston A, Teh M, Ai Z, Wang L, et al. Plasma iron controls neutrophil production and function. Sci Adv. 2022;8:eabq5384 pubmed publisher
Bromley S, Akbaba H, Mani V, Mora Buch R, Chasse A, Sama A, et al. CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell Rep. 2020;32:108085 pubmed publisher
Jackson Jones L, Smith P, Portman J, Magalhaes M, Mylonas K, Vermeren M, et al. Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants. Immunity. 2020;52:700-715.e6 pubmed publisher
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
Clark D, Brazina S, Yang F, Hu D, Hsieh C, Niemi E, et al. Age-related changes to macrophages are detrimental to fracture healing in mice. Aging Cell. 2020;19:e13112 pubmed publisher
Wang B, Dong G, Zhang Q, Yan F, Li Z, Li C, et al. The inhibitor of autophagy SBI-0206965 aggravates atherosclerosis through decreasing myeloid-derived suppressor cells. Exp Ther Med. 2020;19:1370-1378 pubmed publisher
Khorooshi R, Marczynska J, Dieu R, Wais V, Hansen C, Kavan S, et al. Innate signaling within the central nervous system recruits protective neutrophils. Acta Neuropathol Commun. 2020;8:2 pubmed publisher
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
Zhang C, Yue C, Herrmann A, Song J, Egelston C, Wang T, et al. STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth. Cell Metab. 2020;31:148-161.e5 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Hu Y, Wang B, Shen J, Low S, Putt K, Niessen H, et al. Depletion of activated macrophages with a folate receptor-beta-specific antibody improves symptoms in mouse models of rheumatoid arthritis. Arthritis Res Ther. 2019;21:143 pubmed publisher
Movahed E, Cheok Y, Tan G, Lee C, Cheong H, Velayuthan R, et al. Lung-infiltrating T helper 17 cells as the major source of interleukin-17A production during pulmonary Cryptococcus neoformans infection. BMC Immunol. 2018;19:32 pubmed publisher
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
Mollaoglu G, Jones A, Wait S, Mukhopadhyay A, Jeong S, Arya R, et al. The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018;49:764-779.e9 pubmed publisher
Postat J, Olekhnovitch R, Lemaitre F, Bousso P. A Metabolism-Based Quorum Sensing Mechanism Contributes to Termination of Inflammatory Responses. Immunity. 2018;49:654-665.e5 pubmed publisher
Pape K, Maul R, Dileepan T, Paustian A, Gearhart P, Jenkins M. Naive B Cells with High-Avidity Germline-Encoded Antigen Receptors Produce Persistent IgM+ and Transient IgG+ Memory B Cells. Immunity. 2018;48:1135-1143.e4 pubmed publisher
Ritchie N, Ijaz U, Evans T. IL-17 signalling restructures the nasal microbiome and drives dynamic changes following Streptococcus pneumoniae colonization. BMC Genomics. 2017;18:807 pubmed publisher
product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
FC, IP, CMCD, Depletion, IHC, WB
Cat # :
108461
Clone :
RB6-8C5
Item :
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^19. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^20.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity^2, in vivo depletion^3-5,9, immunoprecipitation^1, immunohistochemical staining^6 (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections^11 and zinc-fixed sections^15), and Western blotting^7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)^20.Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA