Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) | BioLegend 108433 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1)

catalog :

108433

quantity :

125 μL

price :

165 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

RB6-8C5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig e8c, e8d	Mastorakos P, Mihelson N, Luby M, Burks S, Johnson K, Hsia A, et al. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021;24:245-258 pubmed publisher
flow cytometry; mouse; loading ...; fig s16d	Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
flow cytometry; mouse	Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
	Rogawski D, Deng J, Li H, Miao H, Borkin D, Purohit T, et al. Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity. Nat Commun. 2021;12:2792 pubmed publisher
	Wei Z, Zhang X, Yong T, Bie N, Zhan G, Li X, et al. Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles. Nat Commun. 2021;12:440 pubmed publisher
	Kalafati L, Kourtzelis I, Schulte Schrepping J, Li X, Hatzioannou A, Grinenko T, et al. Innate Immune Training of Granulopoiesis Promotes Anti-tumor Activity. Cell. 2020;183:771-785.e12 pubmed publisher
	Alikhanyan K, Chen Y, Kraut S, Sotillo R. Targeting alveolar macrophages shows better treatment response than deletion of interstitial macrophages in EGFR mutant lung adenocarcinoma. Immun Inflamm Dis. 2020;8:181-187 pubmed publisher
	Stotesbury C, Alves Peixoto P, Montoya B, Ferez M, Nair S, Snyder C, et al. α2β1 Integrin Is Required for Optimal NK Cell Proliferation during Viral Infection but Not for Acquisition of Effector Functions or NK Cell-Mediated Virus Control. J Immunol. 2020;204:1582-1591 pubmed publisher
	Zhao Y, Lu F, Ye J, Ji M, Pang Y, Wang Y, et al. Myeloid-Derived Suppressor Cells and γδT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol. 2019;10:3104 pubmed publisher
	Wong E, Montoya B, Stotesbury C, Ferez M, Xu R, Sigal L. Langerhans Cells Orchestrate the Protective Antiviral Innate Immune Response in the Lymph Node. Cell Rep. 2019;29:3047-3059.e3 pubmed publisher
	Yamauchi T, Masuda T, Canver M, Seiler M, Semba Y, Shboul M, et al. Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS. Cancer Cell. 2018;33:386-400.e5 pubmed publisher
	Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
	Anstine L, Horne T, Horwitz E, Lincoln J. Contribution of Extra-Cardiac Cells in Murine Heart Valves is Age-Dependent. J Am Heart Assoc. 2017;6: pubmed publisher
	Swarnkar G, Shim K, Nasir A, Seehra K, Chen H, Mbalaviele G, et al. Myeloid Deletion of Nemo Causes Osteopetrosis in Mice Owing to Upregulation of Transcriptional Repressors. Sci Rep. 2016;6:29896 pubmed publisher
	Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
	Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
	Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
	Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
	Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed

product information

Antigen :

Ly-6G Ly-6C

Apps. Abbrev. :

Flow Cytometry

Cat # :

108433

Clone :

RB6-8C5

Item :

Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1)

Isotype :

Rat IgG2b, κ

Other Names :

Gr-1

Size :

125 μL

Price (USD) :

165 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

Raised against granulocytes of mouse origin

Application Notes :

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 108436).

more info or order :

BioLegend product webpage