Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1) | BioLegend 108431 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1)

catalog :

108431

quantity :

125 μL

price :

198 USD

clonality :

monoclonal

host :

rat

conjugate :

Brilliant Violet 570

clone name :

RB6-8C5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s7c	Baryawno N, Przybylski D, Kowalczyk M, Kfoury Y, Severe N, Gustafsson K, et al. A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. Cell. 2019;177:1915-1932.e16 pubmed publisher
flow cytometry; mouse	Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
	Kusnadi A, Park S, Yuan R, Pannellini T, Giannopoulou E, Oliver D, et al. The Cytokine TNF Promotes Transcription Factor SREBP Activity and Binding to Inflammatory Genes to Activate Macrophages and Limit Tissue Repair. Immunity. 2019;51:241-257.e9 pubmed publisher
	Sykes D, Kfoury Y, Mercier F, Wawer M, Law J, Haynes M, et al. Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. Cell. 2016;167:171-186.e15 pubmed publisher
	Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
	Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
	Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
	Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
	Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed

product information

Antigen :

Ly-6G Ly-6C

Apps. Abbrev. :

Flow Cytometry

Cat # :

108431

Clone :

RB6-8C5

Item :

Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1)

Isotype :

Rat IgG2b, κ

Other Names :

Gr-1

Size :

125 μL

Price (USD) :

198 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV570

Immunogen :

Raised against granulocytes of mouse origin

Application Notes :

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 108436).

more info or order :

BioLegend product webpage