Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) | BioLegend 108430 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)

catalog :

108430

quantity :

100 μg

price :

195 USD

clonality :

monoclonal

host :

rat

conjugate :

Pacific Blue

clone name :

RB6-8C5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig s5a	Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig s1d	Kumar B, Garcia M, Weng L, Jung X, Murakami J, Hu X, et al. Acute myeloid leukemia transforms the bone marrow niche into a leukemia-permissive microenvironment through exosome secretion. Leukemia. 2018;32:575-587 pubmed publisher
flow cytometry; mouse	Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
	Domingues A, Kulkarni R, Giotopoulos G, Gupta S, Vinnenberg L, Arede L, et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. elife. 2020;9: pubmed publisher
	Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
	Keklikoglou I, Cianciaruso C, Güç E, Squadrito M, Spring L, Tazzyman S, et al. Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nat Cell Biol. 2019;21:190-202 pubmed publisher
	Schneider C, O Leary C, von Moltke J, Liang H, Ang Q, Turnbaugh P, et al. A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling. Cell. 2018;174:271-284.e14 pubmed publisher
	Reismann D, Stefanowski J, Gunther R, Rakhymzhan A, Matthys R, Nützi R, et al. Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature. Nat Commun. 2017;8:2153 pubmed publisher
	Arima Y, Ohki T, Nishikawa N, Higuchi K, Ota M, Tanaka Y, et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. elife. 2017;6: pubmed publisher
	MANICONE A, Gharib S, Gong K, Eddy W, Long M, Frevert C, et al. Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis. Am J Pathol. 2017;187:1288-1300 pubmed publisher
	Billroth MacLurg A, Ford J, Rosenberg A, Miller J, Fowell D. Regulatory T Cell Numbers in Inflamed Skin Are Controlled by Local Inflammatory Cues That Upregulate CD25 and Facilitate Antigen-Driven Local Proliferation. J Immunol. 2016;197:2208-18 pubmed publisher
	Georgoudaki A, Prokopec K, Boura V, Hellqvist E, Sohn S, Östling J, et al. Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis. Cell Rep. 2016;15:2000-11 pubmed publisher
	Aguado B, Wu J, Azarin S, Nanavati D, Rao S, Bushnell G, et al. Secretome identification of immune cell factors mediating metastatic cell homing. Sci Rep. 2015;5:17566 pubmed publisher
	Engel O, AkyÃ¼z L, da Costa Goncalves A, Winek K, Dames C, Thielke M, et al. Cholinergic Pathway Suppresses Pulmonary Innate Immunity Facilitating Pneumonia After Stroke. Stroke. 2015;46:3232-40 pubmed publisher
	Rosen D, Hilliard J, Tiemann K, Todd E, Morley S, Hunstad D. Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia. J Infect Dis. 2016;213:649-58 pubmed publisher
	Dow L, Nasr Z, Saborowski M, Ebbesen S, Manchado E, Tasdemir N, et al. Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. PLoS ONE. 2014;9:e95236 pubmed publisher
	Yan J, Ralston M, Meng X, Bongiovanni K, Jones A, Benndorf R, et al. Glutathione reductase is essential for host defense against bacterial infection. Free Radic Biol Med. 2013;61:320-32 pubmed publisher
	Hildebrand D, Alexander E, Hörber S, Lehle S, Obermayer K, Münck N, et al. I?B? is a transcriptional key regulator of CCL2/MCP-1. J Immunol. 2013;190:4812-20 pubmed publisher
	Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
	Taube C, Tertilt C, Gyülveszi G, Dehzad N, Kreymborg K, Schneeweiss K, et al. IL-22 is produced by innate lymphoid cells and limits inflammation in allergic airway disease. PLoS ONE. 2011;6:e21799 pubmed publisher
	Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
	Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
	Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
	Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed

product information

Antigen :

Ly-6G Ly-6C

Apps. Abbrev. :

Flow Cytometry

Cat # :

108430

Clone :

RB6-8C5

Item :

Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)

Isotype :

Rat IgG2b, κ

Other Names :

Gr-1

Size :

100 μg

Price (USD) :

195 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

Raised against granulocytes of mouse origin

Application Notes :

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

more info or order :

BioLegend product webpage