product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108429
quantity :
25 μg
price :
94 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Chavez J, Rabe J, Hernandez G, Mills T, Niño K, Davizon Castillo P, et al. PU.1 Expression Defines Distinct Functional Activities in the Phenotypic HSC Compartment of a Murine Inflammatory Stress Model. Cells. 2022;11: pubmed publisher
Beauchamp E, Leventhal M, Bernard E, Hoppe E, Todisco G, Creignou M, et al. ZBTB33 is mutated in clonal hematopoiesis and myelodysplastic syndromes and impacts RNA splicing. Blood Cancer Discov. 2021;2:500-517 pubmed publisher
Melo Silva C, Alves Peixoto P, Heath N, Tang L, Montoya B, Knudson C, et al. Resistance to lethal ectromelia virus infection requires Type I interferon receptor in natural killer cells and monocytes but not in adaptive immune or parenchymal cells. PLoS Pathog. 2021;17:e1009593 pubmed publisher
Szafran B, Borazjani A, Seay C, Carr R, Lehner R, Kaplan B, et al. Effects of Chlorpyrifos on Serine Hydrolase Activities, Lipid Mediators, and Immune Responses in Lungs of Neonatal and Adult Mice. Chem Res Toxicol. 2021;34:1556-1571 pubmed publisher
Fan Z, Mikulski Z, McArdle S, Sundd P, Ley K. Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy. STAR Protoc. 2020;1:100012 pubmed publisher
Domingues A, Kulkarni R, Giotopoulos G, Gupta S, Vinnenberg L, Arede L, et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. elife. 2020;9: pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Guo J, Muse E, Christians A, Swanson S, Davila E. An Anticancer Drug Cocktail of Three Kinase Inhibitors Improved Response to a Dendritic Cell-Based Cancer Vaccine. Cancer Immunol Res. 2019;7:1523-1534 pubmed publisher
Zieman A, Poll B, Ma J, Coulombe P. Altered keratinocyte differentiation is an early driver of keratin mutation-based palmoplantar keratoderma. Hum Mol Genet. 2019;28:2255-2270 pubmed publisher
Orzalli M, Smith A, Jurado K, Iwasaki A, Garlick J, Kagan J. An Antiviral Branch of the IL-1 Signaling Pathway Restricts Immune-Evasive Virus Replication. Mol Cell. 2018;71:825-840.e6 pubmed publisher
Schneider C, O Leary C, von Moltke J, Liang H, Ang Q, Turnbaugh P, et al. A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling. Cell. 2018;174:271-284.e14 pubmed publisher
Reismann D, Stefanowski J, Gunther R, Rakhymzhan A, Matthys R, Nützi R, et al. Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature. Nat Commun. 2017;8:2153 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Arima Y, Ohki T, Nishikawa N, Higuchi K, Ota M, Tanaka Y, et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. elife. 2017;6: pubmed publisher
MANICONE A, Gharib S, Gong K, Eddy W, Long M, Frevert C, et al. Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis. Am J Pathol. 2017;187:1288-1300 pubmed publisher
Billroth MacLurg A, Ford J, Rosenberg A, Miller J, Fowell D. Regulatory T Cell Numbers in Inflamed Skin Are Controlled by Local Inflammatory Cues That Upregulate CD25 and Facilitate Antigen-Driven Local Proliferation. J Immunol. 2016;197:2208-18 pubmed publisher
Georgoudaki A, Prokopec K, Boura V, Hellqvist E, Sohn S, Östling J, et al. Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis. Cell Rep. 2016;15:2000-11 pubmed publisher
Aguado B, Wu J, Azarin S, Nanavati D, Rao S, Bushnell G, et al. Secretome identification of immune cell factors mediating metastatic cell homing. Sci Rep. 2015;5:17566 pubmed publisher
Engel O, Akyüz L, da Costa Goncalves A, Winek K, Dames C, Thielke M, et al. Cholinergic Pathway Suppresses Pulmonary Innate Immunity Facilitating Pneumonia After Stroke. Stroke. 2015;46:3232-40 pubmed publisher
Rosen D, Hilliard J, Tiemann K, Todd E, Morley S, Hunstad D. Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia. J Infect Dis. 2016;213:649-58 pubmed publisher
Dow L, Nasr Z, Saborowski M, Ebbesen S, Manchado E, Tasdemir N, et al. Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. PLoS ONE. 2014;9:e95236 pubmed publisher
Yan J, Ralston M, Meng X, Bongiovanni K, Jones A, Benndorf R, et al. Glutathione reductase is essential for host defense against bacterial infection. Free Radic Biol Med. 2013;61:320-32 pubmed publisher
Hildebrand D, Alexander E, Hörber S, Lehle S, Obermayer K, Münck N, et al. I?B? is a transcriptional key regulator of CCL2/MCP-1. J Immunol. 2013;190:4812-20 pubmed publisher
Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
Taube C, Tertilt C, Gyülveszi G, Dehzad N, Kreymborg K, Schneeweiss K, et al. IL-22 is produced by innate lymphoid cells and limits inflammation in allergic airway disease. PLoS ONE. 2011;6:e21799 pubmed publisher
Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed
product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
Flow Cytometry
Cat # :
108429
Clone :
RB6-8C5
Item :
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
25 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 108436).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA