PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) | BioLegend 108428 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)

catalog :

108428

quantity :

100 μg

price :

205 USD

clonality :

monoclonal

host :

rat

conjugate :

PerCP/Cy5.5

clone name :

RB6-8C5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 5	Vela Ramirez J, Goodman J, Boggiatto P, Roychoudhury R, Pohl N, Hostetter J, et al. Safety and biocompatibility of carbohydrate-functionalized polyanhydride nanoparticles. AAPS J. 2015;17:256-67 pubmed publisher
flow cytometry; mouse	Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
	Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
	Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
	Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
	Giambra V, Gusscott S, Gracias D, Song R, Lam S, Panelli P, et al. Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity. Cell Stem Cell. 2018;23:714-726.e7 pubmed publisher
	Winter C, Silvestre Roig C, Ortega Gomez A, Lemnitzer P, Poelman H, Schumski A, et al. Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell Metab. 2018;28:175-182.e5 pubmed publisher
	Petriello M, Brandon J, Hoffman J, Wang C, Tripathi H, Abdel Latif A, et al. Dioxin-like PCB 126 increases systemic inflammation and accelerates atherosclerosis in lean LDL receptor deficient mice. Toxicol Sci. 2017;: pubmed publisher
	Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
	Hönes J, Thivakaran A, Botezatu L, Patnana P, Castro S, Al Matary Y, et al. Enforced GFI1 expression impedes human and murine leukemic cell growth. Sci Rep. 2017;7:15720 pubmed publisher
	Arcipowski K, Bulic M, Gurbuxani S, Licht J. Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis. PLoS ONE. 2016;11:e0162515 pubmed publisher
	Matsushita N, Suzuki M, Ikebe E, Nagashima S, Inatome R, Asano K, et al. Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. Sci Rep. 2016;6:31266 pubmed publisher
	Guo H, Cooper S, Friedman A. In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS ONE. 2016;11:e0150809 pubmed publisher
	DÃ¤britz J, Judd L, Chalinor H, Menheniott T, Giraud A. Altered gp130 signalling ameliorates experimental colitis via myeloid cell-specific STAT3 activation and myeloid-derived suppressor cells. Sci Rep. 2016;6:20584 pubmed publisher
	Richardson E, Shukla S, Nagy N, Boom W, Beck R, Zhou L, et al. ERK Signaling Is Essential for Macrophage Development. PLoS ONE. 2015;10:e0140064 pubmed publisher
	Li L, Zhang T, Diao W, Jin F, Shi L, Meng J, et al. Role of Myeloid-Derived Suppressor Cells in Glucocorticoid-Mediated Amelioration of FSGS. J Am Soc Nephrol. 2015;26:2183-97 pubmed publisher
	Garcia Bonilla L, Moore J, Racchumi G, Zhou P, Butler J, Iadecola C, et al. Inducible nitric oxide synthase in neutrophils and endothelium contributes to ischemic brain injury in mice. J Immunol. 2014;193:2531-7 pubmed publisher
	Li L, Zhang J, Diao W, Wang D, Wei Y, Zhang C, et al. MicroRNA-155 and MicroRNA-21 promote the expansion of functional myeloid-derived suppressor cells. J Immunol. 2014;192:1034-43 pubmed publisher
	Mishra A, Alves J, Krumbach K, Nigou J, Castro A, Geurtsen J, et al. Differential arabinan capping of lipoarabinomannan modulates innate immune responses and impacts T helper cell differentiation. J Biol Chem. 2012;287:44173-83 pubmed publisher
	Haka A, Potteaux S, Fraser H, Randolph G, Maxfield F. Quantitative analysis of monocyte subpopulations in murine atherosclerotic plaques by multiphoton microscopy. PLoS ONE. 2012;7:e44823 pubmed
	Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
	Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
	Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
	Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
	Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed

product information

Antigen :

Ly-6G Ly-6C

Apps. Abbrev. :

Flow Cytometry

Cat # :

108428

Clone :

RB6-8C5

Item :

PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)

Isotype :

Rat IgG2b, κ

Other Names :

Gr-1

Size :

100 μg

Price (USD) :

205 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Raised against granulocytes of mouse origin

Application Notes :

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

more info or order :

BioLegend product webpage