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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108427
quantity :
25 μg
price :
105 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP/Cy5.5
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 5
Vela Ramirez J, Goodman J, Boggiatto P, Roychoudhury R, Pohl N, Hostetter J, et al. Safety and biocompatibility of carbohydrate-functionalized polyanhydride nanoparticles. AAPS J. 2015;17:256-67 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Wang R, Chen J, Wang W, Zhao Z, Wang H, Liu S, et al. CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model. J Immunother Cancer. 2022;10: pubmed publisher
Giampazolias E, Schulz O, Lim K, Rogers N, Chakravarty P, Srinivasan N, et al. Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunity. Cell. 2021;184:4016-4031.e22 pubmed publisher
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
Dallari S, Heaney T, Rosas Villegas A, Neil J, Wong S, Brown J, et al. Enteric viruses evoke broad host immune responses resembling those elicited by the bacterial microbiome. Cell Host Microbe. 2021;29:1014-1029.e8 pubmed publisher
Chatterjee D, Lewis F, Sutton H, Kaczmarski J, Gao X, Cai Y, et al. Avid binding by B cells to the Plasmodium circumsporozoite protein repeat suppresses responses to protective subdominant epitopes. Cell Rep. 2021;35:108996 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Chen L, Zhang J, Zou Y, Wang F, Li J, Sun F, et al. Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus. Cell Death Differ. 2021;28:1880-1899 pubmed publisher
Kim J, Kolesnikov M, Peled Hajaj S, Scheyltjens I, Xia Y, Trzebanski S, et al. A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages. Immunity. 2021;54:176-190.e7 pubmed publisher
Montel Hagen A, Sun V, Casero D, Tsai S, Zampieri A, Jackson N, et al. In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells. Cell Rep. 2020;33:108320 pubmed publisher
Kobayashi H, Takubo K. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protoc. 2020;1:100078 pubmed publisher
Tripathi H, Al Darraji A, Abo Aly M, Peng H, Shokri E, Chelvarajan L, et al. Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction. J Mol Cell Cardiol. 2020;149:95-114 pubmed publisher
Jamali A, Hu K, Sendra V, Blanco T, Lopez M, Ortiz G, et al. Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis. Cell Rep. 2020;32:108099 pubmed publisher
Tran S, Baba I, Poupel L, Dussaud S, Moreau M, Gélineau A, et al. Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis. Immunity. 2020;53:627-640.e5 pubmed publisher
Wang X, Luo X, Chen C, Tang Y, Li L, Mo B, et al. The Ap-2α/Elk-1 axis regulates Sirpα-dependent tumor phagocytosis by tumor-associated macrophages in colorectal cancer. Signal Transduct Target Ther. 2020;5:35 pubmed publisher
Lee H, Lee Y, Harenda Q, Pietrzak S, Oktay H, Schreiber S, et al. Beta Cell Dedifferentiation Induced by IRE1α Deletion Prevents Type 1 Diabetes. Cell Metab. 2020;31:822-836.e5 pubmed publisher
Lang J, Bohn P, Bhat H, Jastrow H, Walkenfort B, Cansiz F, et al. Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. Nat Commun. 2020;11:1338 pubmed publisher
Tripathi H, Peng H, Donahue R, Chelvarajan L, Gottipati A, Levitan B, et al. Isolation Methods for Human CD34 Subsets Using Fluorescent and Magnetic Activated Cell Sorting: an In Vivo Comparative Study. Stem Cell Rev Rep. 2020;16:413-423 pubmed publisher
Lutzmann M, Bernex F, Da Costa De Jesus C, Hodroj D, Marty C, Plo I, et al. MCM8- and MCM9 Deficiencies Cause Lifelong Increased Hematopoietic DNA Damage Driving p53-Dependent Myeloid Tumors. Cell Rep. 2019;28:2851-2865.e4 pubmed publisher
Wang X, Sun G, Feng T, Zhang J, Huang X, Wang T, et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Res. 2019;29:787-803 pubmed publisher
Zheng Q, Liang S, Xu F, Li G, Luo N, Wu S, et al. C5a/C5aR1 Pathway Is Critical for the Pathogenesis of Psoriasis. Front Immunol. 2019;10:1866 pubmed publisher
Kobayashi H, Morikawa T, Okinaga A, Hamano F, Hashidate Yoshida T, Watanuki S, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28:145-158.e9 pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
Stivala S, Codilupi T, Brkic S, Baerenwaldt A, Ghosh N, Hao Shen H, et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest. 2019;130:1596-1611 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Wang H, Lin X, Luo Y, Sun S, Tian X, Sun Y, et al. α-PD-L1 mAb enhances the abscopal effect of hypo-fractionated radiation by attenuating PD-L1 expression and inducing CD8+ T-cell infiltration. Immunotherapy. 2019;11:101-118 pubmed publisher
Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
Giambra V, Gusscott S, Gracias D, Song R, Lam S, Panelli P, et al. Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity. Cell Stem Cell. 2018;23:714-726.e7 pubmed publisher
Winter C, Silvestre Roig C, Ortega Gomez A, Lemnitzer P, Poelman H, Schumski A, et al. Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell Metab. 2018;28:175-182.e5 pubmed publisher
Petriello M, Brandon J, Hoffman J, Wang C, Tripathi H, Abdel Latif A, et al. Dioxin-like PCB 126 increases systemic inflammation and accelerates atherosclerosis in lean LDL receptor deficient mice. Toxicol Sci. 2017;: pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Hönes J, Thivakaran A, Botezatu L, Patnana P, Castro S, Al Matary Y, et al. Enforced GFI1 expression impedes human and murine leukemic cell growth. Sci Rep. 2017;7:15720 pubmed publisher
Shen L, Yang Y, Ou T, Key C, Tong S, Sequeira R, et al. Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy. J Lipid Res. 2017;58:1808-1821 pubmed publisher
Qi S, Li H, Lu L, Qi Z, Liu L, Chen L, et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. elife. 2016;5: pubmed publisher
Arcipowski K, Bulic M, Gurbuxani S, Licht J. Loss of Mll3 Catalytic Function Promotes Aberrant Myelopoiesis. PLoS ONE. 2016;11:e0162515 pubmed publisher
Matsushita N, Suzuki M, Ikebe E, Nagashima S, Inatome R, Asano K, et al. Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. Sci Rep. 2016;6:31266 pubmed publisher
Guo H, Cooper S, Friedman A. In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS ONE. 2016;11:e0150809 pubmed publisher
Däbritz J, Judd L, Chalinor H, Menheniott T, Giraud A. Altered gp130 signalling ameliorates experimental colitis via myeloid cell-specific STAT3 activation and myeloid-derived suppressor cells. Sci Rep. 2016;6:20584 pubmed publisher
Richardson E, Shukla S, Nagy N, Boom W, Beck R, Zhou L, et al. ERK Signaling Is Essential for Macrophage Development. PLoS ONE. 2015;10:e0140064 pubmed publisher
Li L, Zhang T, Diao W, Jin F, Shi L, Meng J, et al. Role of Myeloid-Derived Suppressor Cells in Glucocorticoid-Mediated Amelioration of FSGS. J Am Soc Nephrol. 2015;26:2183-97 pubmed publisher
Garcia Bonilla L, Moore J, Racchumi G, Zhou P, Butler J, Iadecola C, et al. Inducible nitric oxide synthase in neutrophils and endothelium contributes to ischemic brain injury in mice. J Immunol. 2014;193:2531-7 pubmed publisher
Li L, Zhang J, Diao W, Wang D, Wei Y, Zhang C, et al. MicroRNA-155 and MicroRNA-21 promote the expansion of functional myeloid-derived suppressor cells. J Immunol. 2014;192:1034-43 pubmed publisher
Mishra A, Alves J, Krumbach K, Nigou J, Castro A, Geurtsen J, et al. Differential arabinan capping of lipoarabinomannan modulates innate immune responses and impacts T helper cell differentiation. J Biol Chem. 2012;287:44173-83 pubmed publisher
Haka A, Potteaux S, Fraser H, Randolph G, Maxfield F. Quantitative analysis of monocyte subpopulations in murine atherosclerotic plaques by multiphoton microscopy. PLoS ONE. 2012;7:e44823 pubmed
Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed
product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
Flow Cytometry
Cat # :
108427
Clone :
RB6-8C5
Item :
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
25 μg
Price (USD) :
105 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 108436).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA