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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108425
quantity :
25 μg
price :
99 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Yang Y, Zhang M, Zhang Y, Liu K, Lu C. 5-Fluorouracil Suppresses Colon Tumor through Activating the p53-Fas Pathway to Sensitize Myeloid-Derived Suppressor Cells to FasL+ Cytotoxic T Lymphocyte Cytotoxicity. Cancers (Basel). 2023;15: pubmed publisher
Dolfi B, Gallerand A, Firulyova M, Xu Y, Merlin J, Dumont A, et al. Unravelling the sex-specific diversity and functions of adrenal gland macrophages. Cell Rep. 2022;39:110949 pubmed publisher
Xu J, Yuan L, Hu C, Weng C, Xu H, Shi Y, et al. Trametes robiniophila Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment. Front Pharmacol. 2022;13:911663 pubmed publisher
Friedman D, Kizerwetter M, Belmonte P, Rajcula M, Theodore K, Kim Lee H, et al. Cutting Edge: Enhanced Antitumor Immunity in ST8Sia6 Knockout Mice. J Immunol. 2022;208:1845-1850 pubmed publisher
Wu L, Xu Y, Zhao H, Zhou Y, Chen Y, Yang S, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12:842-858 pubmed publisher
Yu H, Bai Y, Qiu J, He X, Xiong J, Dai Q, et al. Pseudomonas aeruginosa PcrV Enhances the Nitric Oxide-Mediated Tumoricidal Activity of Tumor-Associated Macrophages via a TLR4/PI3K/AKT/mTOR-Glycolysis-Nitric Oxide Circuit. Front Oncol. 2021;11:736882 pubmed publisher
Andreou T, Williams J, Brownlie R, Salmond R, Watson E, Shaw G, et al. Hematopoietic stem cell gene therapy targeting TGFβ enhances the efficacy of irradiation therapy in a preclinical glioblastoma model. J Immunother Cancer. 2021;9: pubmed publisher
Ruer Laventie J, Jayachandran R, Schmaler M, Rossi S, Pieters J. Skin Transplantation and Lymphoid Organ Analysis in Mice. Bio Protoc. 2020;10:e3531 pubmed publisher
Fu W, Li H, Fu H, Zhao S, Shi W, Sun M, et al. The SIRT3 and SIRT6 Promote Prostate Cancer Progression by Inhibiting Necroptosis-Mediated Innate Immune Response. J Immunol Res. 2020;2020:8820355 pubmed publisher
Shemer A, Scheyltjens I, Frumer G, Kim J, Grozovski J, Ayanaw S, et al. Interleukin-10 Prevents Pathological Microglia Hyperactivation following Peripheral Endotoxin Challenge. Immunity. 2020;53:1033-1049.e7 pubmed publisher
Dey S, Sutanto Ward E, Kopp K, DuHadaway J, Mondal A, Ghaban D, et al. Peptide vaccination directed against IDO1-expressing immune cells elicits CD8+ and CD4+ T-cell-mediated antitumor immunity and enhanced anti-PD1 responses. J Immunother Cancer. 2020;8: pubmed publisher
Karki R, Sharma B, Lee E, Banoth B, Malireddi R, Samir P, et al. Interferon regulatory factor 1 regulates PANoptosis to prevent colorectal cancer. JCI Insight. 2020;5: pubmed publisher
Wu L, Zhang X, Zheng L, Zhao H, Yan G, Zhang Q, et al. RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis. Cancer Immunol Res. 2020;8:710-721 pubmed publisher
Chun E, Lavoie S, Fonseca Pereira D, Bae S, Michaud M, Hoveyda H, et al. Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity. Immunity. 2019;51:871-884.e6 pubmed publisher
Chen P, Qin L, Li G, Wang Z, Dahlman J, Malagon Lopez J, et al. Endothelial TGF-β signalling drives vascular inflammation and atherosclerosis. Nat Metab. 2019;1:912-926 pubmed publisher
Akk A, Springer L, Yang L, Hamilton Burdess S, Lambris J, Yan H, et al. Complement activation on neutrophils initiates endothelial adhesion and extravasation. Mol Immunol. 2019;114:629-642 pubmed publisher
Jayachandran R, Gumienny A, Bolinger B, Ruehl S, Lang M, Fucile G, et al. Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity. 2019;50:152-165.e8 pubmed publisher
Cossio Ayala M, Domínguez López M, Mendez Enriquez E, Portillo Téllez M, García Hernández E. In vitro and in vivo antimicrobial activity of a synthetic peptide derived from the C-terminal region of human chemokine CCL13 against Pseudomonas aeruginosa. Peptides. 2017;94:49-55 pubmed publisher
Nenasheva T, Nikolaev A, Diykanov D, Sukhanova A, Tcyganov E, Panteleev A, et al. The introduction of mesenchymal stromal cells induces different immunological responses in the lungs of healthy and M. tuberculosis infected mice. PLoS ONE. 2017;12:e0178983 pubmed publisher
Stubelius A, Andersson A, Islander U, Carlsten H. Ovarian hormones in innate inflammation. Immunobiology. 2017;222:878-883 pubmed publisher
Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, et al. Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-Induced Hematopoietic Injury. Int J Mol Sci. 2017;18: pubmed publisher
Man S, Karki R, Sasai M, Place D, Kesavardhana S, Temirov J, et al. IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes. Cell. 2016;167:382-396.e17 pubmed publisher
Collins S, Chan Li Y, Oh M, Vigeland C, Limjunyawong N, Mitzner W, et al. Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis. JCI Insight. 2016;1:e83116 pubmed
Steinmetz O, Hoch S, Avniel Polak S, Gavish K, Eli Berchoer L, Wilensky A, et al. CX3CR1hi Monocyte/Macrophages Support Bacterial Survival and Experimental Infection-Driven Bone Resorption. J Infect Dis. 2016;213:1505-15 pubmed publisher
Kim P, Xia Juan X, Crump K, Abe T, Hajishengallis G, Sahingur S. Toll-Like Receptor 9-Mediated Inflammation Triggers Alveolar Bone Loss in Experimental Murine Periodontitis. Infect Immun. 2015;83:2992-3002 pubmed publisher
Siegfried A, Berchtold S, Manncke B, Deuschle E, Reber J, Ott T, et al. IFIT2 is an effector protein of type I IFN-mediated amplification of lipopolysaccharide (LPS)-induced TNF-? secretion and LPS-induced endotoxin shock. J Immunol. 2013;191:3913-21 pubmed publisher
Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
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Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed
product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
FC
Cat # :
108425
Clone :
RB6-8C5
Item :
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
25 μg
Price (USD) :
99 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^19. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^20.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity^2, in vivo depletion^3-5,9, immunoprecipitation^1, immunohistochemical staining^6 (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections^11 and zinc-fixed sections^15), and Western blotting^7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)^20.Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA