product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108415
quantity :
25 μg
price :
88 USD
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 83
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...
Yang P, Qin H, Li Y, Xiao A, Zheng E, Zeng H, et al. CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis. Nat Commun. 2022;13:5782 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s20b
Zhang M, Cui J, Lee D, Yuen V, Chiu D, Goh C, et al. Hypoxia-induced macropinocytosis represents a metabolic route for liver cancer. Nat Commun. 2022;13:954 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Ellman D, Degn M, Lund M, Clausen B, Novrup H, Flæng S, et al. Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice. Mediators Inflamm. 2016;2016:2684098 pubmed publisher
  • flow cytometry; mouse; fig 1c
Clausen B, Degn M, Sivasaravanaparan M, Fogtmann T, Andersen M, Trojanowsky M, et al. Conditional ablation of myeloid TNF increases lesion volume after experimental stroke in mice, possibly via altered ERK1/2 signaling. Sci Rep. 2016;6:29291 pubmed publisher
  • flow cytometry; mouse
Mall C, Sckisel G, Proia D, Mirsoian A, Grossenbacher S, Pai C, et al. Repeated PD-1/PD-L1 monoclonal antibody administration induces fatal xenogeneic hypersensitivity reactions in a murine model of breast cancer. Oncoimmunology. 2016;5:e1075114 pubmed
  • flow cytometry; mouse; fig s4
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; mouse
Xu G, Wu H, Zhang J, Li D, Wang Y, Wang Y, et al. Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free Radic Biol Med. 2015;87:15-25 pubmed publisher
  • flow cytometry; mouse; fig 11
Pioli P, Dahlem T, Weis J, Weis J. Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. PLoS ONE. 2013;8:e69216 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Mawambo G, Oubaha M, Ichiyama Y, Blot G, Crespo Garcia S, Dejda A, et al. HIF1α-dependent hypoxia response in myeloid cells requires IRE1α. J Neuroinflammation. 2023;20:145 pubmed publisher
McNamara M, Loyfer N, Kiliti A, Schmidt M, Shabi Porat S, Jain S, et al. Circulating cell-free methylated DNA reveals tissue-specific, cellular damage from radiation treatment. JCI Insight. 2023;8: pubmed publisher
Li G, Gu L, Zhao F, Hu Y, Wang X, Zeng F, et al. WFDC12-overexpressing contributes to the development of atopic dermatitis via accelerating ALOX12/15 metabolism and PAF accumulation. Cell Death Dis. 2023;14:185 pubmed publisher
Liu P, Chen Y, Li X, Hsueh P, Tzeng S, Chen H, et al. CD40 signal rewires fatty acid and glutamine metabolism for stimulating macrophage anti-tumorigenic functions. Nat Immunol. 2023;24:452-462 pubmed publisher
Sch xf6 nberger K, Mitterer M, Gläser K, Stecher M, Hobitz S, Schain Zota D, et al. LC-MS-Based Targeted Metabolomics for FACS-Purified Rare Cells. Anal Chem. 2023;95:4325-4334 pubmed publisher
S xe1 da Bandeira D, Kilpatrick A, Marques M, Gomez Salazar M, Ventura T, Gonzalez Z, et al. PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny. Cell Rep. 2022;40:111114 pubmed publisher
Schnoegl D, Hochgerner M, Gotthardt D, Marsh L. Fra-2 Is a Dominant Negative Regulator of Natural Killer Cell Development. Front Immunol. 2022;13:909270 pubmed publisher
Sch xf6 nberger K, Mitterer M, Buescher J, Cabezas Wallscheid N. Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers. STAR Protoc. 2022;3:101408 pubmed publisher
Chae C, Sandoval T, Hwang S, Park E, Giovanelli P, Awasthi D, et al. Tumor-Derived Lysophosphatidic Acid Blunts Protective Type I Interferon Responses in Ovarian Cancer. Cancer Discov. 2022;12:1904-1921 pubmed publisher
Zhang C, Ostrander E, Kukhar O, Mallaney C, Sun J, Haussler E, et al. Txnip Enhances Fitness of Dnmt3a-Mutant Hematopoietic Stem Cells via p21. Blood Cancer Discov. 2022;3:220-239 pubmed publisher
Wang Y, Zhang W, Xu Y, Wu D, Gao Z, Zhou J, et al. Extracellular HMGB1 Impairs Macrophage-Mediated Efferocytosis by Suppressing the Rab43-Controlled Cell Surface Transport of CD91. Front Immunol. 2022;13:767630 pubmed publisher
Georgiev G, Malonis R, Wirchnianski A, Wessel A, Jung H, Cahill S, et al. Resurfaced ZIKV EDIII nanoparticle immunogens elicit neutralizing and protective responses in vivo. Cell Chem Biol. 2022;29:811-823.e7 pubmed publisher
Bhattacharya P, Ismail N, Saxena A, Gannavaram S, Dey R, Oljuskin T, et al. Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity. PLoS Negl Trop Dis. 2022;16:e0010224 pubmed publisher
Wang B, Varela Eirin M, Brandenburg S, Hernandez Segura A, van Vliet T, Jongbloed E, et al. Pharmacological CDK4/6 inhibition reveals a p53-dependent senescent state with restricted toxicity. EMBO J. 2022;:e108946 pubmed publisher
Aimaitijiang A, Tabu K, Wang W, Nobuhisa I, Taga T. Glioma cells remotely promote erythropoiesis as a self-expanding strategy of cancer stem cells. Genes Cells. 2022;27:25-42 pubmed publisher
Schönberger K, Obier N, Romero Mulero M, Cauchy P, Mess J, Pavlovich P, et al. Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identity. Cell Stem Cell. 2022;29:131-148.e10 pubmed publisher
Wang S, Ren D, Arkoun B, Kaushik A, Matherat G, Lecluse Y, et al. Lyl-1 regulates primitive macrophages and microglia development. Commun Biol. 2021;4:1382 pubmed publisher
Jakob L, M xfc ller T, Rassner M, Kleinfelder H, Veratti P, Mitschke J, et al. Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR. Int J Mol Sci. 2021;22: pubmed publisher
Lawson H, van de Lagemaat L, Barile M, Tavosanis A, Durko J, Villacreces A, et al. CITED2 coordinates key hematopoietic regulatory pathways to maintain the HSC pool in both steady-state hematopoiesis and transplantation. Stem Cell Reports. 2021;16:2784-2797 pubmed publisher
Brusilovsky M, Rochman M, Rochman Y, Caldwell J, Mack L, Felton J, et al. Environmental allergens trigger type 2 inflammation through ripoptosome activation. Nat Immunol. 2021;22:1316-1326 pubmed publisher
Manriquez V, Nivoit P, Urbina T, Echenique Rivera H, Melican K, Fernandez gerlinger M, et al. Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils. Nat Commun. 2021;12:4547 pubmed publisher
Zhao B, Yang J, He B, Li X, Yan H, Liu S, et al. A safe and effective mucosal RSV vaccine in mice consisting of RSV phosphoprotein and flagellin variant. Cell Rep. 2021;36:109401 pubmed publisher
Yu K, Hammerschmidt S, Permanyer M, Galla M, Rothe M, Zheng X, et al. Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable immune synapses. Cell Rep. 2021;35:109273 pubmed publisher
Yang F, He Z, Duan H, Zhang D, Li J, Yang H, et al. Synergistic immunotherapy of glioblastoma by dual targeting of IL-6 and CD40. Nat Commun. 2021;12:3424 pubmed publisher
Mehta C, Fraga de Andrade I, Matson D, Dewey C, Bresnick E. RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis. Nucleic Acids Res. 2021;49:9007-9025 pubmed publisher
Dá Mesquita S, Herz J, Wall M, Dykstra T, de Lima K, Norris G, et al. Aging-associated deficit in CCR7 is linked to worsened glymphatic function, cognition, neuroinflammation, and β-amyloid pathology. Sci Adv. 2021;7: pubmed publisher
Riedel S, Lu C, Xie H, Nestler K, Vermunt M, Lenard A, et al. Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML. Mol Cell. 2021;81:2332-2348.e9 pubmed publisher
Gu Z, Liu Y, Zhang Y, Cao H, Lyu J, Wang X, et al. Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. Nat Genet. 2021;53:672-682 pubmed publisher
Fu Z, Zhang X, Zhou X, Ur Rehman U, Yu M, Liang H, et al. In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Cell Res. 2021;31:631-648 pubmed publisher
Stahlhut M, Ha T, Takmakova E, Morgan M, Schwarzer A, Schaudien D, et al. Conditionally immortalised leukaemia initiating cells co-expressing Hoxa9/Meis1 demonstrate microenvironmental adaptation properties ex vivo while maintaining myelomonocytic memory. Sci Rep. 2021;11:5294 pubmed publisher
Phadke M, Chen Z, Li J, Mohamed E, Davies M, Smalley I, et al. Targeted Therapy Given after Anti-PD-1 Leads to Prolonged Responses in Mouse Melanoma Models through Sustained Antitumor Immunity. Cancer Immunol Res. 2021;9:554-567 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Magee J, Signer R. Developmental Stage-Specific Changes in Protein Synthesis Differentially Sensitize Hematopoietic Stem Cells and Erythroid Progenitors to Impaired Ribosome Biogenesis. Stem Cell Reports. 2021;16:20-28 pubmed publisher
Nagamachi A, Kanai A, Nakamura M, Okuda H, Yokoyama A, Shinriki S, et al. Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. J Clin Invest. 2021;131: pubmed publisher
Badgujar D, Anil A, Green A, Surve M, Madhavan S, Beckett A, et al. Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle. PLoS Pathog. 2020;16:e1009016 pubmed publisher
Dagher T, Maslah N, Edmond V, Cassinat B, Vainchenker W, Giraudier S, et al. JAK2V617F myeloproliferative neoplasm eradication by a novel interferon/arsenic therapy involves PML. J Exp Med. 2021;218: pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
Kotaki R, Kawashima M, Yamaguchi A, Suzuki N, Koyama Nasu R, Ogiya D, et al. Overexpression of miR-669m inhibits erythroblast differentiation. Sci Rep. 2020;10:13554 pubmed publisher
Tran N, Trombke J, Rajewsky K, Chu V. Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells. STAR Protoc. 2020;1:100028 pubmed publisher
Casellas Román H, Venugopal K, Feng Y, Shabashvili D, Posada L, Li J, et al. DNMT3A alterations associated with myeloid malignancies dictate differential responses to hypomethylating agents. Leuk Res. 2020;94:106372 pubmed publisher
Gordon R, Tilstra J, Marinov A, Nickerson K, Bastacky S, Shlomchik M. Murine lupus is neutrophil elastase-independent in the MRL.Faslpr model. PLoS ONE. 2020;15:e0226396 pubmed publisher
Varikuti S, Singh B, Volpedo G, Ahirwar D, Jha B, Saljoughian N, et al. Ibrutinib treatment inhibits breast cancer progression and metastasis by inducing conversion of myeloid-derived suppressor cells to dendritic cells. Br J Cancer. 2020;122:1005-1013 pubmed publisher
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, et al. Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Nat Commun. 2020;11:485 pubmed publisher
Hidalgo San Jose L, Sunshine M, Dillingham C, Chua B, Krutá M, Hong Y, et al. Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal. Cell Rep. 2020;30:69-80.e6 pubmed publisher
Tran N, Sommermann T, Graf R, Trombke J, Pempe J, Petsch K, et al. Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells. Cell Rep. 2019;28:3510-3522.e5 pubmed publisher
Paris J, Morgan M, Campos J, Spencer G, Shmakova A, Ivanova I, et al. Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia. Cell Stem Cell. 2019;: pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Katsumura K, Mehta C, Hewitt K, Soukup A, Fraga de Andrade I, Ranheim E, et al. Human leukemia mutations corrupt but do not abrogate GATA-2 function. Proc Natl Acad Sci U S A. 2018;115:E10109-E10118 pubmed publisher
Li A, Barsoumian H, Schoenhals J, Cushman T, Caetano M, Wang X, et al. Indoleamine 2,3-dioxygenase 1 inhibition targets anti-PD1-resistant lung tumors by blocking myeloid-derived suppressor cells. Cancer Lett. 2018;431:54-63 pubmed publisher
Peltzer N, Darding M, Montinaro A, Dráber P, Draberova H, Kupka S, et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature. 2018;557:112-117 pubmed publisher
Draijer C, Robbe P, Boorsma C, Hylkema M, Melgert B. Dual role of YM1+ M2 macrophages in allergic lung inflammation. Sci Rep. 2018;8:5105 pubmed publisher
Hirata Y, Furuhashi K, Ishii H, Li H, Pinho S, Ding L, et al. CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine. Cell Stem Cell. 2018;22:445-453.e5 pubmed publisher
Syed I, Lee J, Moraes Vieira P, Donaldson C, Sontheimer A, Aryal P, et al. Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab. 2018;27:419-427.e4 pubmed publisher
Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, et al. Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-Induced Hematopoietic Injury. Int J Mol Sci. 2017;18: pubmed publisher
Bussières Marmen S, Vinette V, Gungabeesoon J, Aubry I, Pérez Quintero L, Tremblay M. Loss of T-cell protein tyrosine phosphatase in the intestinal epithelium promotes local inflammation by increasing colonic stem cell proliferation. Cell Mol Immunol. 2018;15:367-376 pubmed publisher
Hartwig T, Montinaro A, von Karstedt S, Sevko A, Surinova S, Chakravarthy A, et al. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2. Mol Cell. 2017;65:730-742.e5 pubmed publisher
Bignami F, Lorusso A, Rama P, Ferrari G. Growth inhibition of formed corneal neovascularization following Fosaprepitant treatment. Acta Ophthalmol. 2017;95:e641-e648 pubmed publisher
Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, Barozzi I, et al. TET2 Regulates Mast Cell Differentiation and Proliferation through Catalytic and Non-catalytic Activities. Cell Rep. 2016;15:1566-1579 pubmed publisher
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Xie X, Yang P, Zhang Y, Zhang P, Wang L, Ding Y, et al. Discovery of novel INK4C small-molecule inhibitors to promote human and murine hematopoietic stem cell ex vivo expansion. Sci Rep. 2015;5:18115 pubmed publisher
Madsen P, Clausen B, Degn M, Thyssen S, Kristensen L, Svensson M, et al. Genetic ablation of soluble tumor necrosis factor with preservation of membrane tumor necrosis factor is associated with neuroprotection after focal cerebral ischemia. J Cereb Blood Flow Metab. 2016;36:1553-69 pubmed publisher
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product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
FC
Cat # :
108415
Clone :
RB6-8C5
Item :
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
25 μg
Price (USD) :
88 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^19. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^20.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity^2, in vivo depletion^3-5,9, immunoprecipitation^1, immunohistochemical staining^6 (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections^11 and zinc-fixed sections^15), and Western blotting^7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)^20.Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA