This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108413
quantity :
50 μg
price :
40 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
RB6-8C5
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
citations: 144
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1d
Kamioka Y, Takakura K, Sumiyama K, Matsuda M. Intravital Förster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli. Cancer Sci. 2017;108:226-235 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h
Rombouts M, Cools N, Grootaert M, de Bakker F, Van Brussel I, Wouters A, et al. Long-Term Depletion of Conventional Dendritic Cells Cannot Be Maintained in an Atherosclerotic Zbtb46-DTR Mouse Model. PLoS ONE. 2017;12:e0169608 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s8b
Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest. 2017;127:383-401 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Murakami S, Shahbazian D, Surana R, Zhang W, Chen H, Graham G, et al. Yes-associated protein mediates immune reprogramming in pancreatic ductal adenocarcinoma. Oncogene. 2017;36:1232-1244 pubmed publisher
  • flow cytometry; mouse; 1:2000; loading ...; fig 2b
Lang S, Harre U, Purohit P, Dietel K, Kienhöfer D, Hahn J, et al. Neurodegeneration Enhances the Development of Arthritis. J Immunol. 2017;198:2394-2402 pubmed publisher
  • flow cytometry; mouse; fig 1a
Taylor S, Huang Y, Mallett G, Stathopoulou C, Felizardo T, Sun M, et al. PD-1 regulates KLRG1+ group 2 innate lymphoid cells. J Exp Med. 2017;214:1663-1678 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Yanagita T, Murata Y, Tanaka D, Motegi S, Arai E, Daniwijaya E, et al. Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy. JCI Insight. 2017;2:e89140 pubmed publisher
  • flow cytometry; mouse; fig 3
Ishiguro T, Fukawa T, Akaki K, Nagaoka K, Takeda T, Iwakura Y, et al. Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice. Eur J Immunol. 2017;47:704-712 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4g
Wang X, Chen H, Tian R, Zhang Y, Drutskaya M, Wang C, et al. Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
  • flow cytometry; mouse; fig S7
Peteranderl C, Morales Nebreda L, Selvakumar B, Lecuona E, Vadász I, Morty R, et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest. 2016;126:1566-80 pubmed publisher
  • flow cytometry; mouse; fig 1
Lasiglié D, Boero S, Bauer I, Morando S, Damonte P, Cea M, et al. Sirt6 regulates dendritic cell differentiation, maturation, and function. Aging (Albany NY). 2016;8:34-49 pubmed
  • flow cytometry; mouse; loading ...; fig s2a
Swanson P, Hart G, Russo M, Nayak D, Yazew T, Pena M, et al. CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature. PLoS Pathog. 2016;12:e1006022 pubmed publisher
  • flow cytometry; mouse; fig 1c
Clausen B, Degn M, Sivasaravanaparan M, Fogtmann T, Andersen M, Trojanowsky M, et al. Conditional ablation of myeloid TNF increases lesion volume after experimental stroke in mice, possibly via altered ERK1/2 signaling. Sci Rep. 2016;6:29291 pubmed publisher
  • flow cytometry; mouse; 30 ug/ml; fig s1
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
  • flow cytometry; mouse; fig s4
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; mouse
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Ellman D, Degn M, Lund M, Clausen B, Novrup H, Flæng S, et al. Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice. Mediators Inflamm. 2016;2016:2684098 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...
Baudiß K, de Paula Vieira R, Cicko S, Ayata K, Hossfeld M, Ehrat N, et al. C1P Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Preventing NF-κB Activation in Neutrophils. J Immunol. 2016;196:2319-26 pubmed publisher
  • flow cytometry; mouse; fig 6
Goetz B, An W, Mohapatra B, Zutshi N, Iseka F, Storck M, et al. A novel CBL-Bflox/flox mouse model allows tissue-selective fully conditional CBL/CBL-B double-knockout: CD4-Cre mediated CBL/CBL-B deletion occurs in both T-cells and hematopoietic stem cells. Oncotarget. 2016;7:51107-51123 pubmed publisher
  • flow cytometry; mouse
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • flow cytometry; mouse
Jones D, Wilmore J, Allman D. Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells. J Immunol. 2015;195:4753-9 pubmed publisher
  • other; mouse; 1:100; fig 5c
Skrzypek K, Kusienicka A, Szewczyk B, Adamus T, Lukasiewicz E, Miekus K, et al. Constitutive activation of MET signaling impairs myogenic differentiation of rhabdomyosarcoma and promotes its development and progression. Oncotarget. 2015;6:31378-98 pubmed publisher
  • flow cytometry; mouse; fig s2
Kitur K, Parker D, Nieto P, Ahn D, Cohen T, Chung S, et al. Toxin-induced necroptosis is a major mechanism of Staphylococcus aureus lung damage. PLoS Pathog. 2015;11:e1004820 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Iwai H, Funatogawa K, Matsumura K, Kato Miyazawa M, Kirikae F, Kiga K, et al. MicroRNA-155 knockout mice are susceptible to Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2015;95:246-50 pubmed publisher
  • flow cytometry; mouse
Spada R, Rojas J, Pérez Yagüe S, Mulens V, Cannata Ortiz P, Bragado R, et al. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol. 2015;97:583-98 pubmed publisher
  • flow cytometry; mouse
Aparicio Domingo P, Romera Hernandez M, Karrich J, Cornelissen F, Papazian N, Lindenbergh Kortleve D, et al. Type 3 innate lymphoid cells maintain intestinal epithelial stem cells after tissue damage. J Exp Med. 2015;212:1783-91 pubmed publisher
  • flow cytometry; mouse; fig 6
Evans E, Jonason A, Bussler H, Torno S, Veeraraghavan J, Reilly C, et al. Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies. Cancer Immunol Res. 2015;3:689-701 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 9
Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, et al. Novel Autoantigens Associated with Lupus Nephritis. PLoS ONE. 2015;10:e0126564 pubmed publisher
  • flow cytometry; mouse
Martin R, Saleem S, Folgosa L, Zellner H, Damle S, Nguyen G, et al. Mast cell histamine promotes the immunoregulatory activity of myeloid-derived suppressor cells. J Leukoc Biol. 2014;96:151-9 pubmed publisher
  • flow cytometry; mouse; fig 6a
Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1,3,4,6
Sauter K, Pridans C, Sehgal A, Bain C, Scott C, Moffat L, et al. The MacBlue binary transgene (csf1r-gal4VP16/UAS-ECFP) provides a novel marker for visualisation of subsets of monocytes, macrophages and dendritic cells and responsiveness to CSF1 administration. PLoS ONE. 2014;9:e105429 pubmed publisher
  • immunocytochemistry; mouse
Nacer A, Movila A, Sohet F, Girgis N, Gundra U, Loke P, et al. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014;10:e1004528 pubmed publisher
  • flow cytometry; mouse; fig s2
Cremasco V, Woodruff M, Onder L, Cupovic J, Nieves Bonilla J, Schildberg F, et al. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nat Immunol. 2014;15:973-81 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d
McFarland B, Marks M, Rowse A, Fehling S, Gerigk M, Qin H, et al. Loss of SOCS3 in myeloid cells prolongs survival in a syngeneic model of glioma. Oncotarget. 2016;7:20621-35 pubmed publisher
  • flow cytometry; mouse; fig s1
  • flow cytometry; human; loading ...; fig s1
Morshed M, Hlushchuk R, Simon D, Walls A, Obata Ninomiya K, Karasuyama H, et al. NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol. 2014;192:5314-23 pubmed publisher
  • flow cytometry; mouse; fig 4
Rao T, Marks Bluth J, Sullivan J, Gupta M, Chandrakanthan V, Fitch S, et al. High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice. Stem Cell Res. 2015;14:307-22 pubmed publisher
  • flow cytometry; mouse; fig 2
Kim J, Phan T, Nguyen V, Dinh Vu H, Zheng J, Yun M, et al. Salmonella typhimurium Suppresses Tumor Growth via the Pro-Inflammatory Cytokine Interleukin-1β. Theranostics. 2015;5:1328-42 pubmed publisher
  • flow cytometry; mouse; fig 4
Su X, Zhang L, Ye J, Yang L, Li Y, Wang Y. Bone marrow mesenchymal stem cells suppress ascitogenous hepatoma progression in BALB/c mouse through reducing myeloid-derived suppressor cells. Biomed Mater Eng. 2015;25:167-77 pubmed publisher
  • flow cytometry; mouse
Xu G, Wu H, Zhang J, Li D, Wang Y, Wang Y, et al. Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free Radic Biol Med. 2015;87:15-25 pubmed publisher
  • flow cytometry; mouse; fig s1
Stoilova B, Kowenz Leutz E, Scheller M, Leutz A. Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications. PLoS ONE. 2013;8:e65169 pubmed publisher
  • flow cytometry; mouse
Vanoaica L, Richman L, Jaworski M, Darshan D, Luther S, Kühn L. Conditional deletion of ferritin h in mice reduces B and T lymphocyte populations. PLoS ONE. 2014;9:e89270 pubmed publisher
  • flow cytometry; mouse; fig 5
Vela Ramirez J, Goodman J, Boggiatto P, Roychoudhury R, Pohl N, Hostetter J, et al. Safety and biocompatibility of carbohydrate-functionalized polyanhydride nanoparticles. AAPS J. 2015;17:256-67 pubmed publisher
  • flow cytometry; mouse
Mall C, Sckisel G, Proia D, Mirsoian A, Grossenbacher S, Pai C, et al. Repeated PD-1/PD-L1 monoclonal antibody administration induces fatal xenogeneic hypersensitivity reactions in a murine model of breast cancer. Oncoimmunology. 2016;5:e1075114 pubmed
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Liu Z, Ravindranathan R, Kalinski P, Guo Z, Bartlett D. Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy. Nat Commun. 2017;8:14754 pubmed publisher
Zhu Q, Wang H, Wang H, Luo Y, Yu Y, Du Q, et al. Protective effects of ethyl pyruvate on lipopolysaccharide‑induced acute lung injury through inhibition of autophagy in neutrophils. Mol Med Rep. 2017;15:1272-1278 pubmed publisher
Mirotti L, Alberca Custódio R, Gomes E, Rammauro F, de Araujo E, Garcia Calich V, et al. CpG-ODN Shapes Alum Adjuvant Activity Signaling via MyD88 and IL-10. Front Immunol. 2017;8:47 pubmed publisher
Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed publisher
Vannini N, Girotra M, Naveiras O, Nikitin G, Campos V, Giger S, et al. Specification of haematopoietic stem cell fate via modulation of mitochondrial activity. Nat Commun. 2016;7:13125 pubmed publisher
Vegas A, Veiseh O, Doloff J, Ma M, Tam H, Bratlie K, et al. Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates. Nat Biotechnol. 2016;34:345-52 pubmed publisher
Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
Kida T, Ayabe S, Omori K, Nakamura T, Maehara T, Aritake K, et al. Prostaglandin D2 Attenuates Bleomycin-Induced Lung Inflammation and Pulmonary Fibrosis. PLoS ONE. 2016;11:e0167729 pubmed publisher
Lemonnier F, Cairns R, Inoue S, Li W, Dupuy A, Broutin S, et al. The IDH2 R172K mutation associated with angioimmunoblastic T-cell lymphoma produces 2HG in T cells and impacts lymphoid development. Proc Natl Acad Sci U S A. 2016;113:15084-15089 pubmed publisher
Revelo X, Tsai S, Lei H, Luck H, Ghazarian M, Tsui H, et al. Perforin is a novel immune regulator of obesity-related insulin resistance. Diabetes. 2015;64:90-103 pubmed publisher
Edgington Mitchell L, Rautela J, Duivenvoorden H, Jayatilleke K, van der Linden W, Verdoes M, et al. Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer. Oncotarget. 2015;6:27008-22 pubmed publisher
Cruz A, Torrado E, Carmona J, Fraga A, Costa P, Rodrigues F, et al. BCG vaccination-induced long-lasting control of Mycobacterium tuberculosis correlates with the accumulation of a novel population of CD4⁺IL-17⁺TNF⁺IL-2⁺ T cells. Vaccine. 2015;33:85-91 pubmed publisher
Wu J, Zhang R, Tang N, Gong Z, Zhou J, Chen Y, et al. Dopamine inhibits the function of Gr-1+CD115+ myeloid-derived suppressor cells through D1-like receptors and enhances anti-tumor immunity. J Leukoc Biol. 2015;97:191-200 pubmed publisher
Andzinski L, Wu C, Lienenklaus S, Kröger A, Weiss S, Jablonska J. Delayed apoptosis of tumor associated neutrophils in the absence of endogenous IFN-β. Int J Cancer. 2015;136:572-83 pubmed publisher
Shindo Y, Yoshimura K, Kuramasu A, Watanabe Y, Ito H, Kondo T, et al. Combination immunotherapy with 4-1BB activation and PD-1 blockade enhances antitumor efficacy in a mouse model of subcutaneous tumor. Anticancer Res. 2015;35:129-36 pubmed
Fahl S, Harris B, Coffey F, Wiest D. Rpl22 Loss Impairs the Development of B Lymphocytes by Activating a p53-Dependent Checkpoint. J Immunol. 2015;194:200-9 pubmed
Knab L, Ebine K, Chow C, Raza S, Sahai V, Patel A, et al. Snail cooperates with Kras G12D in vivo to increase stem cell factor and enhance mast cell infiltration. Mol Cancer Res. 2014;12:1440-8 pubmed publisher
Liu J, Mercher T, Scholl C, Brumme K, Gilliland D, Zhu N. A functional role for the histone demethylase UTX in normal and malignant hematopoietic cells. Exp Hematol. 2012;40:487-98.e3 pubmed publisher
Yan X, Shichita T, Katsumata Y, Matsuhashi T, Ito H, Ito K, et al. Deleterious effect of the IL-23/IL-17A axis and γδT cells on left ventricular remodeling after myocardial infarction. J Am Heart Assoc. 2012;1:e004408 pubmed publisher
Movita D, Kreefft K, Biesta P, van Oudenaren A, Leenen P, Janssen H, et al. Kupffer cells express a unique combination of phenotypic and functional characteristics compared with splenic and peritoneal macrophages. J Leukoc Biol. 2012;92:723-33 pubmed publisher
Zuo D, Yu X, Guo C, Wang H, Qian J, Yi H, et al. Scavenger receptor A restrains T-cell activation and protects against concanavalin A-induced hepatic injury. Hepatology. 2013;57:228-38 pubmed publisher
Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
Piao Y, Liang J, Holmes L, Henry V, Sulman E, de Groot J. Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a mesenchymal transition. Clin Cancer Res. 2013;19:4392-403 pubmed publisher
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Frese Schaper M, Keil A, Yagita H, Steiner S, Falk W, Schmid R, et al. Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice. Cancer Immunol Immunother. 2014;63:571-80 pubmed
Lupfer C, Thomas P, Kanneganti T. Nucleotide oligomerization and binding domain 2-dependent dendritic cell activation is necessary for innate immunity and optimal CD8+ T Cell responses to influenza A virus infection. J Virol. 2014;88:8946-55 pubmed publisher
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Draper D, Gowdy K, Madenspacher J, Wilson R, Whitehead G, Nakano H, et al. ATP binding cassette transporter G1 deletion induces IL-17-dependent dysregulation of pulmonary adaptive immunity. J Immunol. 2012;188:5327-36 pubmed publisher
Slocombe T, Brown S, Miles K, Gray M, Barr T, Gray D. Plasma cell homeostasis: the effects of chronic antigen stimulation and inflammation. J Immunol. 2013;191:3128-38 pubmed publisher
Thorn M, Point G, Burga R, Nguyen C, Joseph Espat N, Katz S. Liver metastases induce reversible hepatic B cell dysfunction mediated by Gr-1+CD11b+ myeloid cells. J Leukoc Biol. 2014;96:883-94 pubmed publisher
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Spees A, Kingsbury D, Wangdi T, Xavier M, Tsolis R, BAUMLER A. Neutrophils are a source of gamma interferon during acute Salmonella enterica serovar Typhimurium colitis. Infect Immun. 2014;82:1692-7 pubmed publisher
Horlad H, Fujiwara Y, Takemura K, Ohnishi K, Ikeda T, Tsukamoto H, et al. Corosolic acid impairs tumor development and lung metastasis by inhibiting the immunosuppressive activity of myeloid-derived suppressor cells. Mol Nutr Food Res. 2013;57:1046-54 pubmed publisher
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Guo H, Ma O, Friedman A. The Cebpa +37-kb enhancer directs transgene expression to myeloid progenitors and to long-term hematopoietic stem cells. J Leukoc Biol. 2014;96:419-26 pubmed publisher
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Kasamatsu J, Azuma M, Oshiumi H, Morioka Y, Okabe M, Ebihara T, et al. INAM plays a critical role in IFN-γ production by NK cells interacting with polyinosinic-polycytidylic acid-stimulated accessory cells. J Immunol. 2014;193:5199-207 pubmed publisher
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Mishra P, Hsuchou H, Ouyang S, Kastin A, Wu X, Pan W. Loss of astrocytic leptin signaling worsens experimental autoimmune encephalomyelitis. Brain Behav Immun. 2013;34:98-107 pubmed publisher
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Patel B, Wilson M, O Dea K, Takata M. TNF-induced death signaling triggers alveolar epithelial dysfunction in acute lung injury. J Immunol. 2013;190:4274-82 pubmed publisher
Koma T, Yoshimatsu K, Nagata N, Sato Y, Shimizu K, Yasuda S, et al. Neutrophil depletion suppresses pulmonary vascular hyperpermeability and occurrence of pulmonary edema caused by hantavirus infection in C.B-17 SCID mice. J Virol. 2014;88:7178-88 pubmed publisher
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Care A, Ingman W, Moldenhauer L, Jasper M, Robertson S. Ovarian steroid hormone-regulated uterine remodeling occurs independently of macrophages in mice. Biol Reprod. 2014;91:60 pubmed publisher
Liu G, Bi Y, Wang R, Yang H, Zhang Y, Wang X, et al. Targeting S1P1 receptor protects against murine immunological hepatic injury through myeloid-derived suppressor cells. J Immunol. 2014;192:3068-79 pubmed publisher
Wang K, Han G, Dou Y, Wang Y, Liu G, Wang R, et al. Opposite role of tumor necrosis factor receptors in dextran sulfate sodium-induced colitis in mice. PLoS ONE. 2012;7:e52924 pubmed publisher
Maruyama A, Shime H, Takeda Y, Azuma M, Matsumoto M, Seya T. Pam2 lipopeptides systemically increase myeloid-derived suppressor cells through TLR2 signaling. Biochem Biophys Res Commun. 2015;457:445-50 pubmed publisher
Wang Y, Han G, Chen Y, Wang K, Liu G, Wang R, et al. Protective role of tumor necrosis factor (TNF) receptors in chronic intestinal inflammation: TNFR1 ablation boosts systemic inflammatory response. Lab Invest. 2013;93:1024-35 pubmed publisher
Vagima Y, Levy Y, Gur D, Tidhar A, Aftalion M, Abramovich H, et al. Early sensing of Yersinia pestis airway infection by bone marrow cells. Front Cell Infect Microbiol. 2012;2:143 pubmed publisher
Miyamoto Y, Uga H, Tanaka S, Kadowaki M, Ikeda M, Saegusa J, et al. Podoplanin is an inflammatory protein upregulated in Th17 cells in SKG arthritic joints. Mol Immunol. 2013;54:199-207 pubmed publisher
Marttila Ichihara F, Auvinen K, Elima K, Jalkanen S, Salmi M. Vascular adhesion protein-1 enhances tumor growth by supporting recruitment of Gr-1+CD11b+ myeloid cells into tumors. Cancer Res. 2009;69:7875-83 pubmed publisher
Lutz J, Dittmann K, Bösl M, Winkler T, Wienands J, Engels N. Reactivation of IgG-switched memory B cells by BCR-intrinsic signal amplification promotes IgG antibody production. Nat Commun. 2015;6:8575 pubmed publisher
Wegorzewska M, Nijagal A, Wong C, Le T, Lescano N, Tang Q, et al. Fetal intervention increases maternal T cell awareness of the foreign conceptus and can lead to immune-mediated fetal demise. J Immunol. 2014;192:1938-45 pubmed publisher
Sun L, Cornell T, Levine A, Berlin A, Hinkovska Galcheva V, Fleszar A, et al. Dual role of interleukin-10 in the regulation of respiratory syncitial virus (RSV)-induced lung inflammation. Clin Exp Immunol. 2013;172:263-79 pubmed publisher
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product information
Antigen :
Ly-6G/Ly-6C (Gr-1)
Apps. Abbrev. :
Flow Cytometry, IP, CMCD, Depletion, IHC, Western Blotting
Cat # :
108413
Clone :
RB6-8C5
Item :
LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1, Ly6G, Ly6C
Size :
50 μg
Price (USD) :
40 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
LEAF
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 108414). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 108436) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
PubMed ID :
16142239,17062731,17494859,17529982,22965162,22996965
company information
BioLegend
9727 Pacific Heights Blvd.
San Diego, CA 92121
customerserv@biolegend.com
www.biolegend.com
headquarters: USA