product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108412
quantity :
100 μg
price :
94 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 176
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s1g, s1h, s1i, s5f
Liu M, Wu C, Luo S, Hua Q, Chen H, Weng Y, et al. PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleen. J Exp Med. 2022;219: pubmed publisher
  • flow cytometry; mouse; fig s4a
Wang H, Wang Q, Yang C, Guo M, Cui X, Jing Z, et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut Microbes. 2022;14:2027853 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Voisin M, Shrestha E, Rollet C, Nikain C, Josefs T, Mahe M, et al. Inhibiting LXRα phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice. Commun Biol. 2021;4:420 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Guo S, Smeltz R, Nanajian A, Heller R. IL-15/IL-15Rα Heterodimeric Complex as Cancer Immunotherapy in Murine Breast Cancer Models. Front Immunol. 2020;11:614667 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s9h
Bielecki P, Riesenfeld S, Hütter J, Torlai Triglia E, Kowalczyk M, Ricardo Gonzalez R, et al. Skin-resident innate lymphoid cells converge on a pathogenic effector state. Nature. 2021;592:128-132 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2s1a
Peligero Cruz C, Givony T, Sebé Pedrós A, Dobes J, Kadouri N, Nevo S, et al. IL18 signaling promotes homing of mature Tregs into the thymus. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a, s3b, s3c, s4a
Cohen G, Chandran P, Lorsung R, Tomlinson L, Sundby M, Burks S, et al. The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response. Cancers (Basel). 2020;12: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 2b, 5b
Yan D, Wang J, Sun H, Zamani A, Zhang H, Chen W, et al. TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3c
Niemann J, Woller N, Brooks J, Fleischmann Mundt B, Martin N, Kloos A, et al. Molecular retargeting of antibodies converts immune defense against oncolytic viruses into cancer immunotherapy. Nat Commun. 2019;10:3236 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 6s2
Lavoie S, Conway K, Lassen K, Jijon H, Pan H, Chun E, et al. The Crohn's disease polymorphism, ATG16L1 T300A, alters the gut microbiota and enhances the local Th1/Th17 response. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6b
Mollaoglu G, Jones A, Wait S, Mukhopadhyay A, Jeong S, Arya R, et al. The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018;49:764-779.e9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1G
Yu V, Yusuf R, Oki T, Wu J, Saez B, Wang X, et al. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2016;167:1310-1322.e17 pubmed publisher
  • flow cytometry; mouse
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed publisher
  • flow cytometry; mouse; fig 1
Charmsaz S, Beckett K, Smith F, Bruedigam C, Moore A, Al Ejeh F, et al. EphA2 Is a Therapy Target in EphA2-Positive Leukemias but Is Not Essential for Normal Hematopoiesis or Leukemia. PLoS ONE. 2015;10:e0130692 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Iwai H, Funatogawa K, Matsumura K, Kato Miyazawa M, Kirikae F, Kiga K, et al. MicroRNA-155 knockout mice are susceptible to Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2015;95:246-50 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
He Y, Gong F, Jin T, Liu Q, Fang H, Chen Y, et al. Dose-Dependent Effects in Plasma Oncotherapy: Critical In Vivo Immune Responses Missed by In Vitro Studies. Biomolecules. 2023;13: pubmed publisher
Ma X, Liang X, Li Y, Feng Q, Cheng K, Ma N, et al. Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field. Nat Commun. 2023;14:1606 pubmed publisher
Chun J, Park S, Lee M, Ha I, Jeong M. The Sesquiterpene Lactone-Rich Fraction of Inula helenium L. Enhances the Antitumor Effect of Anti-PD-1 Antibody in Colorectal Cancer: Integrative Phytochemical, Transcriptomic, and Experimental Analyses. Cancers (Basel). 2023;15: pubmed publisher
Zhao M, Zhu S, Zhang D, Zhou C, Yang Z, Wang C, et al. Long-lasting postoperative analgesia with local anesthetic-loaded hydrogels prevent tumor recurrence via enhancing CD8+T cell infiltration. J Nanobiotechnology. 2023;21:50 pubmed publisher
Chen Y, Liu Z, An N, Zhang J, Meng W, Wang W, et al. Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells. Stem Cells Int. 2023;2023:7482546 pubmed publisher
Wang D, Li W, Albasha N, Griffin L, Chang H, Amaya L, et al. Long-term exposure to house dust mites accelerates lung cancer development in mice. J Exp Clin Cancer Res. 2023;42:26 pubmed publisher
Shi C, Zhang Q, Yao Y, Zeng F, Du C, Nijiati S, et al. Targeting the activity of T cells by membrane surface redox regulation for cancer theranostics. Nat Nanotechnol. 2023;18:86-97 pubmed publisher
Zhang Z, Chen Y, Zheng Y, Wang L, Shen S, Yang G, et al. Quxie Capsule Alleviates Colitis-associated Colorectal Cancer Through Modulating the Gut Microbiota and Suppressing A. fumigatus-induced Aerobic Glycolysis. Integr Cancer Ther. 2022;21:15347354221138534 pubmed publisher
Matsumura T, Totani H, Gunji Y, Fukuda M, Yokomori R, Deng J, et al. A Myb enhancer-guided analysis of basophil and mast cell differentiation. Nat Commun. 2022;13:7064 pubmed publisher
G xf6 tz P, Azubuike Osu S, Braumandl A, Arnholdt C, K xfc bler M, Richter L, et al. Cobra Venom Factor Boosts Arteriogenesis in Mice. Int J Mol Sci. 2022;23: pubmed publisher
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Poh A, Love C, Chisanga D, Steer J, Baloyan D, Chopin M, et al. Therapeutic inhibition of the SRC-kinase HCK facilitates T cell tumor infiltration and improves response to immunotherapy. Sci Adv. 2022;8:eabl7882 pubmed publisher
Guan X, Sun L, Shen Y, Jin F, Bo X, Zhu C, et al. Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nat Commun. 2022;13:2834 pubmed publisher
Yang N, Gong F, Liu B, Hao Y, Chao Y, Lei H, et al. Magnesium galvanic cells produce hydrogen and modulate the tumor microenvironment to inhibit cancer growth. Nat Commun. 2022;13:2336 pubmed publisher
Leick M, Silva H, Scarf xf2 I, Larson R, Choi B, Bouffard A, et al. Non-cleavable hinge enhances avidity and expansion of CAR-T cells for acute myeloid leukemia. Cancer Cell. 2022;40:494-508.e5 pubmed publisher
Li Q, Cheng X, Zhou C, Tang Y, Li F, Zhang B, et al. Fruquintinib Enhances the Antitumor Immune Responses of Anti-Programmed Death Receptor-1 in Colorectal Cancer. Front Oncol. 2022;12:841977 pubmed publisher
Zhou H, Tu C, Yang P, Li J, Kepp O, Li H, et al. Carbon ion radiotherapy triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy in mice. Oncoimmunology. 2022;11:2057892 pubmed publisher
Tan Y, O Dea K, Patel B, Takata M. Investigation of Microvesicle Uptake by Mouse Lung-marginated Monocytes in vitro. Bio Protoc. 2022;12:e4307 pubmed publisher
Huang D, Wang Y, Thompson J, Yin T, Alexander P, Qin D, et al. Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. Nat Cell Biol. 2022;24:230-241 pubmed publisher
Xiao Y, Chen J, Zhou H, Zeng X, Ruan Z, Pu Z, et al. Combining p53 mRNA nanotherapy with immune checkpoint blockade reprograms the immune microenvironment for effective cancer therapy. Nat Commun. 2022;13:758 pubmed publisher
Fielding C, García García A, Korn C, Gadomski S, Fang Z, Reguera J, et al. Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis. Nat Commun. 2022;13:543 pubmed publisher
Qi Z, Xu Z, Zhang L, Zou Y, Li J, Yan W, et al. Overcoming resistance to immune checkpoint therapy in PTEN-null prostate cancer by intermittent anti-PI3Kα/β/δ treatment. Nat Commun. 2022;13:182 pubmed publisher
Barsoumian H, Sezen D, Menon H, Younes A, Hu Y, He K, et al. High Plus Low Dose Radiation Strategy in Combination with TIGIT and PD1 Blockade to Promote Systemic Antitumor Responses. Cancers (Basel). 2022;14: pubmed publisher
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Tuong Z, Loudon K, Berry B, Richoz N, Jones J, Tan X, et al. Resolving the immune landscape of human prostate at a single-cell level in health and cancer. Cell Rep. 2021;37:110132 pubmed publisher
Schnell A, Huang L, Singer M, Singaraju A, Barilla R, Regan B, et al. Stem-like intestinal Th17 cells give rise to pathogenic effector T cells during autoimmunity. Cell. 2021;184:6281-6298.e23 pubmed publisher
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Zeng J, Wang Y, Luo Z, Chang L, Yoo J, Yan H, et al. TRIM9-Mediated Resolution of Neuroinflammation Confers Neuroprotection upon Ischemic Stroke in Mice. Cell Rep. 2019;27:549-560.e6 pubmed publisher
Agelidis A, Koujah L, Suryawanshi R, Yadavalli T, Mishra Y, Adelung R, et al. An Intra-Vaginal Zinc Oxide Tetrapod Nanoparticles (ZOTEN) and Genital Herpesvirus Cocktail Can Provide a Novel Platform for Live Virus Vaccine. Front Immunol. 2019;10:500 pubmed publisher
Vannini N, Campos V, Girotra M, Trachsel V, Rojas Sutterlin S, Tratwal J, et al. The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance. Cell Stem Cell. 2019;24:405-418.e7 pubmed publisher
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Weckbach L, Grabmaier U, Uhl A, Gess S, Boehm F, Zehrer A, et al. Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis. J Exp Med. 2019;216:350-368 pubmed publisher
Abraham T, Flickinger J, Waldman S, Snook A. TCR Retrogenic Mice as a Model To Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen GUCY2C. J Immunol. 2019;202:1301-1310 pubmed publisher
Gan T, Yang Y, Hu F, Chen X, Zhou J, Li Y, et al. TLR3 Regulated Poly I:C-Induced Neutrophil Extracellular Traps and Acute Lung Injury Partly Through p38 MAP Kinase. Front Microbiol. 2018;9:3174 pubmed publisher
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product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
FC
Cat # :
108412
Clone :
RB6-8C5
Item :
APC anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
100 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C^19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8^19. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4^20.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity^2, in vivo depletion^3-5,9, immunoprecipitation^1, immunohistochemical staining^6 (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections^11 and zinc-fixed sections^15), and Western blotting^7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)^20.Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).;
more info or order :
company information
BioLegend
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San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA