product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108405
quantity :
50 μg
price :
20 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
RB6-8C5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 67
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s19b
Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed publisher
  • flow cytometry; mouse; loading ...
He W, Wang C, Mu R, Liang P, Huang Z, Zhang J, et al. MiR-21 is required for anti-tumor immune response in mice: an implication for its bi-directional roles. Oncogene. 2017;36:4212-4223 pubmed publisher
  • flow cytometry; mouse; fig 3
Ishiguro T, Fukawa T, Akaki K, Nagaoka K, Takeda T, Iwakura Y, et al. Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice. Eur J Immunol. 2017;47:704-712 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
  • flow cytometry; mouse; fig s2
Kitur K, Parker D, Nieto P, Ahn D, Cohen T, Chung S, et al. Toxin-induced necroptosis is a major mechanism of Staphylococcus aureus lung damage. PLoS Pathog. 2015;11:e1004820 pubmed publisher
  • flow cytometry; mouse; fig 4
Su X, Zhang L, Ye J, Yang L, Li Y, Wang Y. Bone marrow mesenchymal stem cells suppress ascitogenous hepatoma progression in BALB/c mouse through reducing myeloid-derived suppressor cells. Biomed Mater Eng. 2015;25:167-77 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, et al. Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Nat Commun. 2020;11:485 pubmed publisher
Rosenbaum S, Knecht M, Mollaee M, Zhong Z, Erkes D, McCue P, et al. FOXD3 Regulates VISTA Expression in Melanoma. Cell Rep. 2020;30:510-524.e6 pubmed publisher
Hidalgo San Jose L, Sunshine M, Dillingham C, Chua B, Krutá M, Hong Y, et al. Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal. Cell Rep. 2020;30:69-80.e6 pubmed publisher
Hong Y, Kim Y, Kim G, Nam G, Kim S, Park Y, et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles. 2019;8:1670893 pubmed publisher
Fang R, Uchiyama R, Sakai S, Hara H, Tsutsui H, Suda T, et al. ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism. Mucosal Immunol. 2019;: pubmed publisher
Saul S, Castelbou C, Fickentscher C, Demaurex N. Signaling and functional competency of neutrophils derived from bone-marrow cells expressing the ER-HOXB8 oncoprotein. J Leukoc Biol. 2019;106:1101-1115 pubmed publisher
Casulli J, Fife M, Houston S, Rossi S, Dow J, Williamson E, et al. CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality. Nat Commun. 2019;10:2121 pubmed publisher
Nowlan B, Williams E, Doran M, Levesque J. CD27, CD201, FLT3, CD48, and CD150 cell surface staining identifies long-term mouse hematopoietic stem cells in immunodeficient non-obese diabetic severe combined immune deficient derived strains. Haematologica. 2019;: pubmed publisher
Uderhardt S, Martins A, Tsang J, Lämmermann T, Germain R. Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 2019;177:541-555.e17 pubmed publisher
Liao W, Overman M, Boutin A, Shang X, Zhao D, Dey P, et al. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer. Cancer Cell. 2019;35:559-572.e7 pubmed publisher
Srivastava S, Salter A, Liggitt D, Yechan Gunja S, Sarvothama M, Cooper K, et al. Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting. Cancer Cell. 2019;35:489-503.e8 pubmed publisher
Codina A, Renauer P, Wang G, Chow R, Park J, Ye H, et al. Convergent Identification and Interrogation of Tumor-Intrinsic Factors that Modulate Cancer Immunity In Vivo. Cell Syst. 2019;8:136-151.e7 pubmed publisher
Wang B, Lim J, Kajikawa T, Li X, Vallance B, Moutsopoulos N, et al. Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis. Cell Rep. 2019;26:1614-1626.e5 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Zegarra Ruiz D, El Beidaq A, Iñiguez A, Lubrano Di Ricco M, Manfredo Vieira S, Ruff W, et al. A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity. Cell Host Microbe. 2019;25:113-127.e6 pubmed publisher
Greenwald A, Licht T, Kumar S, Oladipupo S, Iyer S, Grunewald M, et al. VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells. J Exp Med. 2019;216:215-230 pubmed publisher
Jin X, Ding D, Yan Y, Li H, Wang B, Ma L, et al. Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression. Mol Cell. 2019;73:22-35.e6 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Golan K, Kumari A, Kollet O, Khatib Massalha E, Subramaniam M, Ferreira Z, et al. Daily Onset of Light and Darkness Differentially Controls Hematopoietic Stem Cell Differentiation and Maintenance. Cell Stem Cell. 2018;23:572-585.e7 pubmed publisher
Guo X, Dash P, Crawford J, Allen E, Zamora A, Boyd D, et al. Lung γδ T Cells Mediate Protective Responses during Neonatal Influenza Infection that Are Associated with Type 2 Immunity. Immunity. 2018;49:531-544.e6 pubmed publisher
Xiang W, Shi R, Kang X, Zhang X, Chen P, Zhang L, et al. Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression. Nat Commun. 2018;9:2574 pubmed publisher
Robinett R, Guan N, Lux A, Biburger M, Nimmerjahn F, Meyer A. Dissecting Fc?R Regulation through a Multivalent Binding Model. Cell Syst. 2018;7:41-48.e5 pubmed publisher
Wang Q, Liu F, Zhang M, Zhou P, Xu C, Li Y, et al. NLRP12 Promotes Mouse Neutrophil Differentiation through Regulation of Non-canonical NF-κB and MAPKERK1/2 Signaling. Int J Biol Sci. 2018;14:147-155 pubmed publisher
Zhao N, Cao J, Xu L, Tang Q, Dobrolecki L, Lv X, et al. Pharmacological targeting of MYC-regulated IRE1/XBP1 pathway suppresses MYC-driven breast cancer. J Clin Invest. 2018;128:1283-1299 pubmed publisher
Nagatake T, Shiogama Y, Inoue A, Kikuta J, Honda T, Tiwari P, et al. The 17,18-epoxyeicosatetraenoic acid-G protein-coupled receptor 40 axis ameliorates contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaques. J Allergy Clin Immunol. 2018;142:470-484.e12 pubmed publisher
Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
Cao W, Kayama H, Chen M, Delmas A, Sun A, Kim S, et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids. Immunity. 2017;47:1182-1196.e10 pubmed publisher
Reismann D, Stefanowski J, Gunther R, Rakhymzhan A, Matthys R, Nützi R, et al. Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature. Nat Commun. 2017;8:2153 pubmed publisher
Burton O, Tamayo J, Stranks A, Miller S, Koleoglou K, Weinberg E, et al. IgE promotes type 2 innate lymphoid cells in murine food allergy. Clin Exp Allergy. 2017;: pubmed publisher
Wang C, Zhang N, Qi L, Yuan J, Wang K, Wang K, et al. Myeloid-Derived Suppressor Cells Inhibit T Follicular Helper Cell Immune Response in Japanese Encephalitis Virus Infection. J Immunol. 2017;199:3094-3105 pubmed publisher
Cuff A, Male V. Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 iCreTbx21 fl/fl mice. Wellcome Open Res. 2017;2:39 pubmed publisher
Guan F, Bailey C, Metierre C, O Young P, Gao D, Khoo T, et al. The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo. J Hematol Oncol. 2017;10:75 pubmed publisher
Maicas N, van der Vlag J, Bublitz J, Florquin S, Bakker van Bebber M, Dinarello C, et al. Human Alpha-1-Antitrypsin (hAAT) therapy reduces renal dysfunction and acute tubular necrosis in a murine model of bilateral kidney ischemia-reperfusion injury. PLoS ONE. 2017;12:e0168981 pubmed publisher
Furuhashi K, Chua Y, Wong K, Zhou Q, Lee D, Liong K, et al. Priming with high and low respiratory allergen dose induces differential CD4+ T helper type 2 cells and IgE/IgG1 antibody responses in mice. Immunology. 2017;151:227-238 pubmed publisher
Kitur K, Wachtel S, Brown A, Wickersham M, Paulino F, Peñaloza H, et al. Necroptosis Promotes Staphylococcus aureus Clearance by Inhibiting Excessive Inflammatory Signaling. Cell Rep. 2016;16:2219-2230 pubmed publisher
Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, Barozzi I, et al. TET2 Regulates Mast Cell Differentiation and Proliferation through Catalytic and Non-catalytic Activities. Cell Rep. 2016;15:1566-1579 pubmed publisher
Chen Z, Yu K, Zhu F, Gorczynski R. Over-Expression of CD200 Protects Mice from Dextran Sodium Sulfate Induced Colitis. PLoS ONE. 2016;11:e0146681 pubmed publisher
Wang X, Ma K, Chen M, Ko K, Zheng B, Lu L. IL-17A Promotes Pulmonary B-1a Cell Differentiation via Induction of Blimp-1 Expression during Influenza Virus Infection. PLoS Pathog. 2016;12:e1005367 pubmed publisher
Ish Shalom E, Meirow Y, Sade Feldman M, Kanterman J, Wang L, Mizrahi O, et al. Impaired SNX9 Expression in Immune Cells during Chronic Inflammation: Prognostic and Diagnostic Implications. J Immunol. 2016;196:156-67 pubmed publisher
Zou J, Shankar N. Surface protein Esp enhances pro-inflammatory cytokine expression through NF-κB activation during enterococcal infection. Innate Immun. 2016;22:31-9 pubmed publisher
Zhang J, Li L, Baldwin A, Friedman A, Paz Priel I. Loss of IKKβ but Not NF-κB p65 Skews Differentiation towards Myeloid over Erythroid Commitment and Increases Myeloid Progenitor Self-Renewal and Functional Long-Term Hematopoietic Stem Cells. PLoS ONE. 2015;10:e0130441 pubmed publisher
An J, Li Z, Dong Y, Wu J, Ren J. Complement activation contributes to the anti-methicillin-resistant Staphylococcus aureus effect of natural anti-keratin antibody. Biochem Biophys Res Commun. 2015;461:142-7 pubmed publisher
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product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
Flow Cytometry
Cat # :
108405
Clone :
RB6-8C5
Item :
FITC anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
50 μg
Price (USD) :
20 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA