product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)
catalog :
108404
quantity :
500 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
RB6-8C5
reactivity :
mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4g
Wang X, Chen H, Tian R, Zhang Y, Drutskaya M, Wang C, et al. Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1G
Yu V, Yusuf R, Oki T, Wu J, Saez B, Wang X, et al. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2016;167:1310-1322.e17 pubmed publisher
  • flow cytometry; mouse
Chen J, Miyanishi M, Wang S, Yamazaki S, Sinha R, Kao K, et al. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016;530:223-7 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Domingues A, Kulkarni R, Giotopoulos G, Gupta S, Vinnenberg L, Arede L, et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. elife. 2020;9: pubmed publisher
Hurrell B, Galle Treger L, Jahani P, Howard E, Helou D, Banie H, et al. TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity. Cell Rep. 2019;29:4509-4524.e5 pubmed publisher
Davis F, Schaller M, denDekker A, Joshi A, Kimball A, Evanoff H, et al. Sepsis Induces Prolonged Epigenetic Modifications in Bone Marrow and Peripheral Macrophages Impairing Inflammation and Wound Healing. Arterioscler Thromb Vasc Biol. 2019;39:2353-2366 pubmed publisher
Ito K, Lee J, Chrysanthou S, Zhao Y, Josephs K, Sato H, et al. Non-catalytic Roles of Tet2 Are Essential to Regulate Hematopoietic Stem and Progenitor Cell Homeostasis. Cell Rep. 2019;28:2480-2490.e4 pubmed publisher
Nagashima H, Mahlakõiv T, Shih H, Davis F, Meylan F, Huang Y, et al. Neuropeptide CGRP Limits Group 2 Innate Lymphoid Cell Responses and Constrains Type 2 Inflammation. Immunity. 2019;51:682-695.e6 pubmed publisher
Goldstein J, Tabebordbar M, Zhu K, Wang L, Messemer K, Peacker B, et al. In Situ Modification of Tissue Stem and Progenitor Cell Genomes. Cell Rep. 2019;27:1254-1264.e7 pubmed publisher
Kubota S, Tokunaga K, Umezu T, Yokomizo Nakano T, Sun Y, Oshima M, et al. Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm. Nat Commun. 2019;10:1653 pubmed publisher
Dey A, Yang W, Gegonne A, Nishiyama A, Pan R, Yagi R, et al. BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses. EMBO J. 2019;38: pubmed publisher
Galle Treger L, Sankaranarayanan I, Hurrell B, Howard E, Lo R, Maazi H, et al. Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes. Nat Commun. 2019;10:713 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Patel M, Behar A, Silasi R, Regmi G, Sansam C, Keshari R, et al. Role of ADTRP (Androgen-Dependent Tissue Factor Pathway Inhibitor Regulating Protein) in Vascular Development and Function. J Am Heart Assoc. 2018;7:e010690 pubmed publisher
Säwén P, Eldeeb M, Erlandsson E, Kristiansen T, Laterza C, Kokaia Z, et al. Murine HSCs contribute actively to native hematopoiesis but with reduced differentiation capacity upon aging. elife. 2018;7: pubmed publisher
Mann M, Mehta A, de Boer C, Kowalczyk M, Lee K, Haldeman P, et al. Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with Age. Cell Rep. 2018;25:2992-3005.e5 pubmed publisher
Giambra V, Gusscott S, Gracias D, Song R, Lam S, Panelli P, et al. Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity. Cell Stem Cell. 2018;23:714-726.e7 pubmed publisher
Parada Kusz M, Penaranda C, Hagedorn E, Clatworthy A, Nair A, Henninger J, et al. Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies. Dis Model Mech. 2018;11: pubmed publisher
Tanimura N, Liao R, Wilson G, Dent M, Cao M, Burstyn J, et al. GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism. Dev Cell. 2018;46:581-594.e4 pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Zhu C, Xia L, Li F, Zhou L, Weng Q, Li Z, et al. mTOR complexes differentially orchestrates eosinophil development in allergy. Sci Rep. 2018;8:6883 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Johnson J, Georgakilas G, Petrovic J, Kurachi M, Cai S, Harly C, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells. Immunity. 2018;48:243-257.e10 pubmed publisher
Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
Luo W, Weisel F, Shlomchik M. B Cell Receptor and CD40 Signaling Are Rewired for Synergistic Induction of the c-Myc Transcription Factor in Germinal Center B Cells. Immunity. 2018;48:313-326.e5 pubmed publisher
Zhang C, Yi W, Li F, Du X, Wang H, Wu P, et al. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis. Cell Res. 2018;28:323-335 pubmed publisher
Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Moon H, Donahue L, Choi E, Scumpia P, Lowry W, Grenier J, et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell. 2017;21:665-678.e6 pubmed publisher
Ahmed A, Yim H, Alorro M, Ernst M, Williams B. Integrin-Linked Kinase Expression in Myeloid Cells Promotes Inflammatory Signaling during Experimental Colitis. J Immunol. 2017;: pubmed publisher
Ishidome T, Yoshida T, Hanayama R. Induction of Live Cell Phagocytosis by a Specific Combination of Inflammatory Stimuli. EBioMedicine. 2017;22:89-99 pubmed publisher
Yu V, Yusuf R, Oki T, Wu J, Saez B, Wang X, et al. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2017;168:944-945 pubmed publisher
Sochalska M, Schuler F, Weiss J, Prchal Murphy M, Sexl V, Villunger A. MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene. 2017;36:2066-2073 pubmed publisher
Carr M, Roderick J, Zhang H, Woda B, Kelliher M, Jones S. Phosphorylation of the Mdm2 oncoprotein by the c-Abl tyrosine kinase regulates p53 tumor suppression and the radiosensitivity of mice. Proc Natl Acad Sci U S A. 2016;113:15024-15029 pubmed publisher
Zysset D, Weber B, Rihs S, Brasseit J, Freigang S, Riether C, et al. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nat Commun. 2016;7:13151 pubmed publisher
Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, et al. Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. Nature. 2016;537:698-702 pubmed publisher
Dubeykovskaya Z, Si Y, Chen X, Worthley D, Renz B, Urbanska A, et al. Neural innervation stimulates splenic TFF2 to arrest myeloid cell expansion and cancer. Nat Commun. 2016;7:10517 pubmed publisher
Lutz J, Dittmann K, Bösl M, Winkler T, Wienands J, Engels N. Reactivation of IgG-switched memory B cells by BCR-intrinsic signal amplification promotes IgG antibody production. Nat Commun. 2015;6:8575 pubmed publisher
Kitano M, Yamazaki C, Takumi A, Ikeno T, Hemmi H, Takahashi N, et al. Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node. Proc Natl Acad Sci U S A. 2016;113:1044-9 pubmed publisher
Engels N, König L, Schulze W, Radtke D, Vanshylla K, Lutz J, et al. The immunoglobulin tail tyrosine motif upgrades memory-type BCRs by incorporating a Grb2-Btk signalling module. Nat Commun. 2014;5:5456 pubmed publisher
Roderick J, Hermance N, Zelic M, Simmons M, Polykratis A, Pasparakis M, et al. Hematopoietic RIPK1 deficiency results in bone marrow failure caused by apoptosis and RIPK3-mediated necroptosis. Proc Natl Acad Sci U S A. 2014;111:14436-41 pubmed publisher
Nihon Yanagi Y, Terai K, Murano T, Kawai T, Kimura S, Okazumi S. ?-2 microglobulin is unsuitable as an internal reference gene for the analysis of gene expression in human colorectal cancer. Biomed Rep. 2013;1:193-196 pubmed
Michel A, Schuler A, Friedrich P, Döner F, Bopp T, Radsak M, et al. Mast cell-deficient Kit(W-sh) "Sash" mutant mice display aberrant myelopoiesis leading to the accumulation of splenocytes that act as myeloid-derived suppressor cells. J Immunol. 2013;190:5534-44 pubmed publisher
Piao Y, Liang J, Holmes L, Zurita A, Henry V, Heymach J, et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro Oncol. 2012;14:1379-92 pubmed publisher
Getts D, Terry R, Getts M, Müller M, Rana S, Shrestha B, et al. Ly6c+ "inflammatory monocytes" are microglial precursors recruited in a pathogenic manner in West Nile virus encephalitis. J Exp Med. 2008;205:2319-37 pubmed publisher
Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue Caley R, McHeyzer Williams L, et al. Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007;8:753-61 pubmed
Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, et al. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007;110:1639-47 pubmed
Dzhagalov I, St John A, He Y. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood. 2007;109:1620-6 pubmed
Andoniou C, van Dommelen S, Voigt V, Andrews D, Brizard G, Asselin Paturel C, et al. Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity. Nat Immunol. 2005;6:1011-9 pubmed
product information
Antigen :
Ly-6G Ly-6C
Apps. Abbrev. :
Flow Cytometry, IHC, IP, Western Blotting
Cat # :
108404
Clone :
RB6-8C5
Item :
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)
Isotype :
Rat IgG2b, κ
Other Names :
Gr-1
Size :
500 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BIOTIN
Immunogen :
Raised against granulocytes of mouse origin
Application Notes :
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C 19 . Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8 19 . However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4 20 . The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo . Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity 2 , in vivo depletion 3-5,9 , immunoprecipitation 1 , immunohistochemical staining 6 (including paraffin-embedded sections 9,16,33-35 , acetone-fixed frozen sections 11 and zinc-fixed sections 15 ), and Western blotting 7 . RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs) 20 . Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA