product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse Ly-6A/E (Sca-1)
catalog :
108127
quantity :
125 μL
price :
193 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
D7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4d
Chen J, Sivan U, Tan S, Lippo L, De Angelis J, Labella R, et al. High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1l
Baryawno N, Przybylski D, Kowalczyk M, Kfoury Y, Severe N, Gustafsson K, et al. A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. Cell. 2019;177:1915-1932.e16 pubmed publisher
Zhou T, He C, Lai P, Yang Z, Liu Y, Xu H, et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv. 2022;8:eabj9617 pubmed publisher
Groenen A, La Rose A, Li M, Bazioti V, Svendsen A, Kloosterhuis N, et al. Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease. J Lipid Res. 2022;63:100167 pubmed publisher
Koide S, Sigurdsson V, Radulović V, Saito K, Zheng Z, Lang S, et al. CD244 expression represents functional decline of murine hematopoietic stem cells after in vitro culture. iScience. 2022;25:103603 pubmed publisher
Forte D, Garcia Fernandez M, Sanchez Aguilera A, Stavropoulou V, Fielding C, Martin Perez D, et al. Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy. Cell Metab. 2020;32:829-843.e9 pubmed publisher
JAISWAL A, Makhija S, Stahr N, Sandey M, Suryawanshi A, Mishra A. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation. Immunobiology. 2020;225:151956 pubmed publisher
Runyan C, Welch L, Lecuona E, Shigemura M, Amarelle L, Abdala Valencia H, et al. Impaired phagocytic function in CX3CR1+ tissue-resident skeletal muscle macrophages prevents muscle recovery after influenza A virus-induced pneumonia in old mice. Aging Cell. 2020;: pubmed publisher
Pessoa Rodrigues C, Herman J, Herquel B, Valsecchi C, Stehle T, Grün D, et al. Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate. Sci Adv. 2020;6:eaaz4815 pubmed publisher
Goldsmith J, Spitofsky N, Zamani A, Hood R, Boggs A, Li X, et al. TNFAIP8 controls murine intestinal stem cell homeostasis and regeneration by regulating microbiome-induced Akt signaling. Nat Commun. 2020;11:2591 pubmed publisher
Elahi S, Holling G, Stablewski A, Olejniczak S. Improved hematopoietic differentiation of mouse embryonic stem cells through manipulation of the RNA binding protein ARS2. Stem Cell Res. 2020;43:101710 pubmed publisher
Fukushima T, Tanaka Y, Hamey F, Chang C, Oki T, Asada S, et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep. 2019;29:4144-4158.e7 pubmed publisher
Ho Y, del Toro R, Rivera Torres J, Rak J, Korn C, García García A, et al. Remodeling of Bone Marrow Hematopoietic Stem Cell Niches Promotes Myeloid Cell Expansion during Premature or Physiological Aging. Cell Stem Cell. 2019;25:407-418.e6 pubmed publisher
Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
Yamashita M, Passegue E. TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Radulović V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, et al. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis. Cell Rep. 2019;27:2826-2836.e5 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
Mitchell K, Barreyro L, Todorova T, Taylor S, Antony Debré I, Narayanagari S, et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med. 2018;215:1709-1727 pubmed publisher
Reyna D, Garner T, Lopez A, Kopp F, Choudhary G, Sridharan A, et al. Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia. Cancer Cell. 2017;32:490-505.e10 pubmed publisher
Kotfis K, Zegan Barańska M, Szydłowski Ł, Zukowski M, Ely E. Methods of pain assessment in adult intensive care unit patients - Polish version of the CPOT (Critical Care Pain Observation Tool) and BPS (Behavioral Pain Scale). Anaesthesiol Intensive Ther. 2017;49:66-72 pubmed publisher
Sykes D, Kfoury Y, Mercier F, Wawer M, Law J, Haynes M, et al. Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. Cell. 2016;167:171-186.e15 pubmed publisher
Thongthip S, Bellani M, Gregg S, Sridhar S, Conti B, Chen Y, et al. Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction. Genes Dev. 2016;30:645-59 pubmed publisher
Yuzugullu H, Baitsch L, Von T, Steiner A, Tong H, Ni J, et al. A PI3K p110β-Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis. Nat Commun. 2015;6:8501 pubmed publisher
Nijnik A, Clare S, Hale C, Raisen C, McIntyre R, Yusa K, et al. The critical role of histone H2A-deubiquitinase Mysm1 in hematopoiesis and lymphocyte differentiation. Blood. 2012;119:1370-9 pubmed publisher
Pronk C, Veiby O, Bryder D, Jacobsen S. Tumor necrosis factor restricts hematopoietic stem cell activity in mice: involvement of two distinct receptors. J Exp Med. 2011;208:1563-70 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
product information
Antigen :
Ly-6A/E
Apps. Abbrev. :
Flow Cytometry
Cat # :
108127
Clone :
D7
Item :
Brilliant Violet 421™ anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
125 μL
Price (USD) :
193 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
IL-2-dependent mouse T-cell line (CTL-L)
Application Notes :
The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting 1,2 , immunoprecipitation 1 , in vitro lymphocyte activation 3-6 , induction of redirected lysis 7 , induction of T cell inhibitory signalling 8 , immunofluorescence 9 , and immunohistochemical staining of acetone-fixed frozen sections 13 and Bouin-fixed, paraffin-embedded samples 9 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 14
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA