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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
catalog :
108126
quantity :
100 μg
price :
292 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
D7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:700; loading ...; fig ex3a
McAlpine C, Kiss M, Rattik S, He S, Vassalli A, Valet C, et al. Sleep modulates haematopoiesis and protects against atherosclerosis. Nature. 2019;566:383-387 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3c
Barros Silva J, Linn D, Steiner I, Guo G, Ali A, Pakula H, et al. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer. Cell Rep. 2018;25:3504-3518.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Cai Z, Kotzin J, Ramdas B, Chen S, Nelanuthala S, Palam L, et al. Inhibition of Inflammatory Signaling in Tet2 Mutant Preleukemic Cells Mitigates Stress-Induced Abnormalities and Clonal Hematopoiesis. Cell Stem Cell. 2018;23:833-849.e5 pubmed publisher
  • flow cytometry; mouse; fig 2
Joshi P, Waterhouse P, Kannan N, Narala S, Fang H, Di Grappa M, et al. RANK Signaling Amplifies WNT-Responsive Mammary Progenitors through R-SPONDIN1. Stem Cell Reports. 2015;5:31-44 pubmed publisher
  • flow cytometry; mouse; fig 3
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
Jatho A, Zieseniss A, Brechtel Curth K, Guo J, Böker K, Salinas G, et al. The HIFα-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells. Cells. 2022;11: pubmed publisher
Valdes Mora F, Salomon R, Gloss B, Law A, Venhuizen J, Castillo L, et al. Single-cell transcriptomics reveals involution mimicry during the specification of the basal breast cancer subtype. Cell Rep. 2021;35:108945 pubmed publisher
Heyde A, Rohde D, McAlpine C, Zhang S, Hoyer F, Gerold J, et al. Increased stem cell proliferation in atherosclerosis accelerates clonal hematopoiesis. Cell. 2021;184:1348-1361.e22 pubmed publisher
Ye H, Minhajuddin M, Krug A, Pei S, Chou C, Culp Hill R, et al. The Hepatic Microenvironment Uniquely Protects Leukemia Cells through Induction of Growth and Survival Pathways Mediated by LIPG. Cancer Discov. 2021;11:500-519 pubmed publisher
Ozaki M, Kagawa S, Ishii M, Hegab A. Optimizing the in vitro colony-forming assay for more efficient delineation of the interaction between lung epithelial stem cells and their niche. J Stem Cells Regen Med. 2020;16:50-62 pubmed publisher
Gomzikova M, Aimaletdinov A, Bondar O, Starostina I, Gorshkova N, Neustroeva O, et al. Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells. Sci Rep. 2020;10:10740 pubmed publisher
Cai Z, Aguilera F, Ramdas B, Daulatabad S, Srivastava R, Kotzin J, et al. Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells. Cell Rep. 2020;31:107816 pubmed publisher
Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart B, Brouilly N, et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages. Immunity. 2020;53:127-142.e7 pubmed publisher
Hu B, Lv X, Chen H, Xue P, Gao B, Wang X, et al. Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones. J Clin Invest. 2020;130:3483-3498 pubmed publisher
Hillel Karniel C, Rosen C, Milman Krentsis I, Orgad R, Bachar Lustig E, Shezen E, et al. Multi-lineage Lung Regeneration by Stem Cell Transplantation across Major Genetic Barriers. Cell Rep. 2020;30:807-819.e4 pubmed publisher
Helbling P, Piñeiro Yañez E, Gerosa R, Boettcher S, Al Shahrour F, Manz M, et al. Global Transcriptomic Profiling of the Bone Marrow Stromal Microenvironment during Postnatal Development, Aging, and Inflammation. Cell Rep. 2019;29:3313-3330.e4 pubmed publisher
Gross K, Zhou W, Breindel J, Ouyang J, Jin D, Sokol E, et al. Loss of Slug Compromises DNA Damage Repair and Accelerates Stem Cell Aging in Mammary Epithelium. Cell Rep. 2019;28:394-407.e6 pubmed publisher
Abraham A, Qiu S, Chacko B, Li H, Paterson A, He J, et al. SIRT1 regulates metabolism and leukemogenic potential in CML stem cells. J Clin Invest. 2019;129:2685-2701 pubmed publisher
Paris J, Morgan M, Campos J, Spencer G, Shmakova A, Ivanova I, et al. Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia. Cell Stem Cell. 2019;: pubmed publisher
Joshi P, Waterhouse P, Kasaian K, Fang H, Gulyaeva O, Sul H, et al. PDGFRα+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland. Nat Commun. 2019;10:1760 pubmed publisher
Agarwal P, Isringhausen S, Li H, Paterson A, He J, Gomariz Á, et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell. 2019;24:769-784.e6 pubmed publisher
Kobayashi T, Voisin B, Kim D, Kennedy E, Jo J, Shih H, et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell. 2019;176:982-997.e16 pubmed publisher
Hermetet F, Buffiere A, Aznague A, Pais de Barros J, Bastie J, Delva L, et al. High-fat diet disturbs lipid raft/TGF-β signaling-mediated maintenance of hematopoietic stem cells in mouse bone marrow. Nat Commun. 2019;10:523 pubmed publisher
Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Meisel M, Hinterleitner R, Pacis A, Chen L, Earley Z, Mayassi T, et al. Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature. 2018;557:580-584 pubmed publisher
Reismann D, Stefanowski J, Gunther R, Rakhymzhan A, Matthys R, Nützi R, et al. Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature. Nat Commun. 2017;8:2153 pubmed publisher
Naik S, Larsen S, Gomez N, Alaverdyan K, Sendoel A, Yuan S, et al. Inflammatory memory sensitizes skin epithelial stem cells to tissue damage. Nature. 2017;550:475-480 pubmed publisher
Yang S, Li L, Xie Y, Yao Y, Gao C, Liao L, et al. IFN-?-STAT1-iNOS Induces Myeloid Progenitors to Acquire Immunosuppressive Activity. Front Immunol. 2017;8:1192 pubmed publisher
Carr M, Roderick J, Zhang H, Woda B, Kelliher M, Jones S. Phosphorylation of the Mdm2 oncoprotein by the c-Abl tyrosine kinase regulates p53 tumor suppression and the radiosensitivity of mice. Proc Natl Acad Sci U S A. 2016;113:15024-15029 pubmed publisher
Grandl G, Müller S, Moest H, Moser C, Wollscheid B, Wolfrum C. Depot specific differences in the adipogenic potential of precursors are mediated by collagenous extracellular matrix and Flotillin 2 dependent signaling. Mol Metab. 2016;5:937-947 pubmed publisher
Corna G, Caserta I, Monno A, Apostoli P, Manfredi A, Camaschella C, et al. The Repair of Skeletal Muscle Requires Iron Recycling through Macrophage Ferroportin. J Immunol. 2016;197:1914-25 pubmed publisher
Belle J, Petrov J, Langlais D, Robert F, Cencic R, Shen S, et al. Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance. Cell Death Differ. 2016;23:759-75 pubmed publisher
Fu X, Zhu M, Dodson M, Du M. AMP-activated protein kinase stimulates Warburg-like glycolysis and activation of satellite cells during muscle regeneration. J Biol Chem. 2015;290:26445-56 pubmed publisher
Jie Z, Liang Y, Hou L, Dong C, Iwakura Y, Soong L, et al. Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection. J Immunol. 2014;192:3289-300 pubmed publisher
Pronk C, Veiby O, Bryder D, Jacobsen S. Tumor necrosis factor restricts hematopoietic stem cell activity in mice: involvement of two distinct receptors. J Exp Med. 2011;208:1563-70 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
product information
Antigen :
Ly-6A Ly-6E
Apps. Abbrev. :
Flow Cytometry
Cat # :
108126
Clone :
D7
Item :
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
100 μg
Price (USD) :
292 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
IL-2-dependent mouse T-cell line (CTL-L)
Application Notes :
The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting 1,2 , immunoprecipitation 1 , in vitro lymphocyte activation 3-6 , induction of redirected lysis 7 , induction of T cell inhibitory signalling 8 , immunofluorescence 9 , and immunohistochemical staining of acetone-fixed frozen sections 13 and Bouin-fixed, paraffin-embedded samples 9 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 14
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA