product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)
catalog :
108119
quantity :
25 μg
price :
85 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
D7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1a, s9
Halvarsson C, Rörby E, Eliasson P, Lang S, Soneji S, Jönsson J. Putative role of NF-kB but not HIF-1α in hypoxia-dependent regulation of oxidative stress in hematopoietic stem and progenitor cells. Antioxid Redox Signal. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...
Rychtarčíková Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget. 2017;8:6376-6398 pubmed publisher
  • flow cytometry; mouse; 1:75
Quarta M, Brett J, DiMarco R, de Morrée A, Boutet S, Chacon R, et al. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nat Biotechnol. 2016;34:752-9 pubmed publisher
  • flow cytometry; mouse; fig 3
Karamitros D, Patmanidi A, Kotantaki P, Potocnik A, Bähr Ivacevic T, Benes V, et al. Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors. Development. 2015;142:70-81 pubmed publisher
Wosczyna M, Perez Carbajal E, Wagner M, Paredes S, Konishi C, Liu L, et al. Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells. Cell Stem Cell. 2021;28:1323-1334.e8 pubmed publisher
Kenswil K, Pisterzi P, Sanchez Duffhues G, van Dijk C, Lolli A, Knuth C, et al. Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation. Cell Stem Cell. 2021;28:653-670.e11 pubmed publisher
Zhou S, Zhang W, Cai G, Ding Y, Wei C, Li S, et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res. 2020;30:1063-1077 pubmed publisher
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
Di Genua C, Valletta S, Buono M, Stoilova B, Sweeney C, Rodriguez Meira A, et al. C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor. Cancer Cell. 2020;37:690-704.e8 pubmed publisher
Viaud M, Abdel Wahab O, Gall J, Ivanov S, Guinamard R, Sore S, et al. ABCA1 Exerts Tumor-Suppressor Function in Myeloproliferative Neoplasms. Cell Rep. 2020;30:3397-3410.e5 pubmed publisher
Haider N, Larose L. Activation of the PDGFRα-Nrf2 pathway mediates impaired adipocyte differentiation in bone marrow mesenchymal stem cells lacking Nck1. Cell Commun Signal. 2020;18:26 pubmed publisher
He M, Chiang H, Luo H, Zheng Z, Qiao Q, Wang L, et al. An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance. Cell Metab. 2020;31:580-591.e5 pubmed publisher
Hillel Karniel C, Rosen C, Milman Krentsis I, Orgad R, Bachar Lustig E, Shezen E, et al. Multi-lineage Lung Regeneration by Stem Cell Transplantation across Major Genetic Barriers. Cell Rep. 2020;30:807-819.e4 pubmed publisher
Kang Y, Pietras E, Passegue E. Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia. J Exp Med. 2020;217: pubmed publisher
Søndergaard E, Rauch A, Michaut M, Rapin N, Rehn M, Wilhelmson A, et al. ERG Controls B Cell Development by Promoting Igh V-to-DJ Recombination. Cell Rep. 2019;29:2756-2769.e6 pubmed publisher
Yamashita M, Passegue E. TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Luchsinger L, Strikoudis A, Danzl N, Bush E, Finlayson M, Satwani P, et al. Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro. Cell Stem Cell. 2019;: pubmed publisher
Wosczyna M, Konishi C, Perez Carbajal E, Wang T, Walsh R, Gan Q, et al. Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle. Cell Rep. 2019;27:2029-2035.e5 pubmed publisher
Joshi P, Waterhouse P, Kasaian K, Fang H, Gulyaeva O, Sul H, et al. PDGFRα+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland. Nat Commun. 2019;10:1760 pubmed publisher
Di Genua C, Norfo R, Rodriguez Meira A, Wen W, Drissen R, Booth C, et al. Cell-intrinsic depletion of Aml1-ETO-expressing pre-leukemic hematopoietic stem cells by K-Ras activating mutation. Haematologica. 2019;: pubmed publisher
Giordani L, He G, Negroni E, Sakai H, Law J, Siu M, et al. High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations. Mol Cell. 2019;74:609-621.e6 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Xiong X, Schober M, Tassone E, Khodadadi Jamayran A, Sastre Perona A, Zhou H, et al. KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer. Cell Rep. 2018;25:3006-3020.e7 pubmed publisher
Mann M, Mehta A, de Boer C, Kowalczyk M, Lee K, Haldeman P, et al. Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with Age. Cell Rep. 2018;25:2992-3005.e5 pubmed publisher
Hamanaka S, Umino A, Sato H, Hayama T, Yanagida A, Mizuno N, et al. Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation. Stem Cell Reports. 2018;11:988-997 pubmed publisher
Liu L, Charville G, Cheung T, Yoo B, Santos P, Schroeder M, et al. Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells. Cell Stem Cell. 2018;23:544-556.e4 pubmed publisher
Sarshad A, Juan A, Muler A, Anastasakis D, Wang X, Genzor P, et al. Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells. Mol Cell. 2018;71:1040-1050.e8 pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Gao B, Lin X, Jing H, Fan J, Ji C, Jie Q, et al. Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice. Aging Cell. 2018;17:e12741 pubmed publisher
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
Huang Z, Zhong L, Lee J, Zhang J, Wu D, Geng L, et al. The FGF21-CCL11 Axis Mediates Beiging of White Adipose Tissues by Coupling Sympathetic Nervous System to Type 2 Immunity. Cell Metab. 2017;26:493-508.e4 pubmed publisher
Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, et al. Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth. elife. 2017;6: pubmed publisher
Mueller A, van Velthoven C, Fukumoto K, Cheung T, Rando T. Intronic polyadenylation of PDGFR? in resident stem cells attenuates muscle fibrosis. Nature. 2016;540:276-279 pubmed publisher
Jonas B, Johnson C, Gratzinger D, Majeti R. Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity. PLoS ONE. 2016;11:e0159189 pubmed publisher
Dusseault J, Li B, Haider N, Goyette M, Cote J, Larose L. Nck2 Deficiency in Mice Results in Increased Adiposity Associated With Adipocyte Hypertrophy and Enhanced Adipogenesis. Diabetes. 2016;65:2652-66 pubmed publisher
Pietras E, Mirantes Barbeito C, Fong S, Loeffler D, Kovtonyuk L, Zhang S, et al. Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal. Nat Cell Biol. 2016;18:607-18 pubmed publisher
Lebert Ghali C, Fournier M, Kettyle L, Thompson A, Sauvageau G, Bijl J. Hoxa cluster genes determine the proliferative activity of adult mouse hematopoietic stem and progenitor cells. Blood. 2016;127:87-90 pubmed publisher
Alvarez S, Diaz M, Flach J, Rodriguez Acebes S, López Contreras A, Martinez D, et al. Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nat Commun. 2015;6:8548 pubmed publisher
Ambrosio F, Brown E, Stolz D, Ferrari R, Goodpaster B, Deasy B, et al. Arsenic induces sustained impairment of skeletal muscle and muscle progenitor cell ultrastructure and bioenergetics. Free Radic Biol Med. 2014;74:64-73 pubmed publisher
Torreggiani E, Matthews B, Pejda S, Matić I, Horowitz M, Grcevic D, et al. Preosteocytes/osteocytes have the potential to dedifferentiate becoming a source of osteoblasts. PLoS ONE. 2013;8:e75204 pubmed publisher
Pronk C, Veiby O, Bryder D, Jacobsen S. Tumor necrosis factor restricts hematopoietic stem cell activity in mice: involvement of two distinct receptors. J Exp Med. 2011;208:1563-70 pubmed publisher
Aguilo F, Avagyan S, Labar A, Sevilla A, Lee D, Kumar P, et al. Prdm16 is a physiologic regulator of hematopoietic stem cells. Blood. 2011;117:5057-66 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
Grassinger J, Haylock D, Storan M, Haines G, Williams B, Whitty G, et al. Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins. Blood. 2009;114:49-59 pubmed publisher
Nemeth M, Mak K, Yang Y, Bodine D. beta-Catenin expression in the bone marrow microenvironment is required for long-term maintenance of primitive hematopoietic cells. Stem Cells. 2009;27:1109-19 pubmed publisher
Sabnis A, Cheung L, Dail M, Kang H, Santaguida M, Hermiston M, et al. Oncogenic Kras initiates leukemia in hematopoietic stem cells. PLoS Biol. 2009;7:e59 pubmed publisher
Agarwal A, Bumm T, Corbin A, O Hare T, Loriaux M, VanDyke J, et al. Absence of SKP2 expression attenuates BCR-ABL-induced myeloproliferative disease. Blood. 2008;112:1960-70 pubmed publisher
product information
Antigen :
Ly-6A Ly-6E
Apps. Abbrev. :
Flow Cytometry
Cat # :
108119
Clone :
D7
Item :
Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
25 μg
Price (USD) :
85 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
IL-2-dependent mouse T-cell line (CTL-L)
Application Notes :
The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting 1,2 , immunoprecipitation 1 , in vitro lymphocyte activation 3-6 , induction of redirected lysis 7 , induction of T cell inhibitory signalling 8 , immunofluorescence 9 , and immunohistochemical staining of acetone-fixed frozen sections 13 and Bouin-fixed, paraffin-embedded samples 9 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 14
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA