product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse Ly-6A/E (Sca-1)
catalog :
108101
quantity :
50 μg
price :
50 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
D7
reactivity :
mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; loading ...; fig ev3a
Anderson R, Lagnado A, Maggiorani D, Walaszczyk A, Dookun E, Chapman J, et al. Length-independent telomere damage drives post-mitotic cardiomyocyte senescence. EMBO J. 2019;38: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Rowe R, Lummertz da Rocha E, Sousa P, Missios P, Morse M, Marion W, et al. The developmental stage of the hematopoietic niche regulates lineage in MLL-rearranged leukemia. J Exp Med. 2019;216:527-538 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Paudel S, Baral P, Ghimire L, Bergeron S, Jin L, De Corte J, et al. CXCL1 regulates neutrophil homeostasis in pneumonia-derived sepsis caused by Streptococcus pneumoniae serotype 3. Blood. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Heshmati Y, Kharazi S, Türköz G, Chang D, Kamali Dolatabadi E, Boström J, et al. The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation. Sci Rep. 2018;8:11202 pubmed publisher
  • flow cytometry; mouse; fig 2d
Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1e
Lis R, Karrasch C, Poulos M, Kunar B, Redmond D, Duran J, et al. Conversion of adult endothelium to immunocompetent haematopoietic stem cells. Nature. 2017;545:439-445 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Ebner F, Sedlyarov V, Tasciyan S, Ivin M, Kratochvill F, Gratz N, et al. The RNA-binding protein tristetraprolin schedules apoptosis of pathogen-engaged neutrophils during bacterial infection. J Clin Invest. 2017;127:2051-2065 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3c
Bagchi S, He Y, Zhang H, Cao L, Van Rhijn I, Moody D, et al. CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice. J Clin Invest. 2017;127:2339-2352 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Carrieri C, Comazzetto S, Grover A, Morgan M, Buness A, Nerlov C, et al. A transit-amplifying population underpins the efficient regenerative capacity of the testis. J Exp Med. 2017;214:1631-1641 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g, 4a, s8a
Lefrançais E, Ortiz Muñoz G, Caudrillier A, Mallavia B, Liu F, Sayah D, et al. The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors. Nature. 2017;544:105-109 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Stanley R, Piszczatowski R, Bartholdy B, Mitchell K, McKimpson W, Narayanagari S, et al. A myeloid tumor suppressor role for NOL3. J Exp Med. 2017;214:753-771 pubmed publisher
  • flow cytometry; mouse; loading ...; tbl 2
Moestrup K, Andersen M, Jensen K. Isolation and In Vitro Characterization of Epidermal Stem Cells. Methods Mol Biol. 2017;1553:67-83 pubmed publisher
  • flow cytometry; mouse; loading ...
Rychtarčíková Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget. 2017;8:6376-6398 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7e
Connor L, Tang S, Cognard E, Ochiai S, Hilligan K, Old S, et al. Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles. J Exp Med. 2017;214:125-142 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Chu V, Graf R, Wirtz T, Weber T, Favret J, Li X, et al. Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Proc Natl Acad Sci U S A. 2016;113:12514-12519 pubmed
  • flow cytometry; mouse; loading ...; fig st1
Johnston L, Hsu C, Krier Burris R, Chhiba K, Chien K, McKenzie A, et al. IL-33 Precedes IL-5 in Regulating Eosinophil Commitment and Is Required for Eosinophil Homeostasis. J Immunol. 2016;197:3445-3453 pubmed
  • flow cytometry; mouse; 1:100; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; fig 6a
Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • flow cytometry; mouse; 1:75
Quarta M, Brett J, DiMarco R, de Morrée A, Boutet S, Chacon R, et al. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nat Biotechnol. 2016;34:752-9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
  • flow cytometry; mouse; fig s6
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; rat; fig 4
Zhao W, Wang C, Liu R, Wei C, Duan J, Liu K, et al. Effect of TGF-β1 on the Migration and Recruitment of Mesenchymal Stem Cells after Vascular Balloon Injury: Involvement of Matrix Metalloproteinase-14. Sci Rep. 2016;6:21176 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1b
Park J, Park J, Park D, Kim D, Kim H. Stem Cells Antigen-1 Enriches for a Cancer Stem Cell-Like Subpopulation in Mouse Gastric Cancer. Stem Cells. 2016;34:1177-87 pubmed publisher
  • flow cytometry; mouse
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse
Lasigliè D, Boero S, Bauer I, Morando S, Damonte P, Cea M, et al. Sirt6 regulates dendritic cell differentiation, maturation, and function. Aging (Albany NY). 2016;8:34-49 pubmed
  • flow cytometry; mouse; fig 1
Liu L, Cheung T, Charville G, Rando T. Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting. Nat Protoc. 2015;10:1612-24 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3
Kim M, Taparowsky E, Kim C. Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut. Immunity. 2015;43:107-19 pubmed publisher
  • flow cytometry; mouse; fig 3
Imai Y, Ayithan N, Wu X, Yuan Y, Wang L, Hwang S. Cutting Edge: PD-1 Regulates Imiquimod-Induced Psoriasiform Dermatitis through Inhibition of IL-17A Expression by Innate γδ-Low T Cells. J Immunol. 2015;195:421-5 pubmed publisher
  • flow cytometry; mouse; fig s2
Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
  • flow cytometry; mouse; fig 3
Karamitros D, Patmanidi A, Kotantaki P, Potocnik A, Bähr Ivacevic T, Benes V, et al. Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors. Development. 2015;142:70-81 pubmed publisher
  • flow cytometry; mouse; fig 3
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
Krotenberg Garcia A, Fumagalli A, Le H, Jackstadt R, Lannagan T, Sansom O, et al. Active elimination of intestinal cells drives oncogenic growth in organoids. Cell Rep. 2021;36:109307 pubmed publisher
Khan K, Ponce de León J, Benguigui M, Xu P, Chow A, Cruz Munoz W, et al. Immunostimulatory and anti-tumor metronomic cyclophosphamide regimens assessed in primary orthotopic and metastatic murine breast cancer. NPJ Breast Cancer. 2020;6:29 pubmed publisher
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
Karmaus P, Chen X, Lim S, Herrada A, Nguyen T, Xu B, et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature. 2019;565:101-105 pubmed publisher
Gubin M, Esaulova E, Ward J, Malkova O, Runci D, Wong P, et al. High-Dimensional Analysis Delineates Myeloid and Lymphoid Compartment Remodeling during Successful Immune-Checkpoint Cancer Therapy. Cell. 2018;175:1014-1030.e19 pubmed publisher
Paulo E, Wu D, Wang Y, Zhang Y, Wu Y, Swaney D, et al. Sympathetic inputs regulate adaptive thermogenesis in brown adipose tissue through cAMP-Salt inducible kinase axis. Sci Rep. 2018;8:11001 pubmed publisher
Burton O, Tamayo J, Stranks A, Miller S, Koleoglou K, Weinberg E, et al. IgE promotes type 2 innate lymphoid cells in murine food allergy. Clin Exp Allergy. 2017;: pubmed publisher
Reeves P, Abbaslou M, Kools F, Vutipongsatorn K, Tong X, Gavegano C, et al. Ruxolitinib sensitizes ovarian cancer to reduced dose Taxol, limits tumor growth and improves survival in immune competent mice. Oncotarget. 2017;8:94040-94053 pubmed publisher
Sargent A, Bai L, Shano G, Karl M, Garrison E, Ranasinghe L, et al. CNS disease diminishes the therapeutic functionality of bone marrow mesenchymal stem cells. Exp Neurol. 2017;295:222-232 pubmed publisher
Weng L, Hu X, Kumar B, García M, Todorov I, Jung X, et al. Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor. Sci Rep. 2016;6:38632 pubmed publisher
Hu X, García M, Weng L, Jung X, Murakami J, Kumar B, et al. Identification of a common mesenchymal stromal progenitor for the adult haematopoietic niche. Nat Commun. 2016;7:13095 pubmed publisher
Samal R, Ameling S, Wenzel K, Dhople V, Volker U, Felix S, et al. OMICS-based exploration of the molecular phenotype of resident cardiac progenitor cells from adult murine heart. J Proteomics. 2012;75:5304-15 pubmed publisher
Pronk C, Veiby O, Bryder D, Jacobsen S. Tumor necrosis factor restricts hematopoietic stem cell activity in mice: involvement of two distinct receptors. J Exp Med. 2011;208:1563-70 pubmed publisher
product information
Antigen :
Ly-6A Ly-6E
Apps. Abbrev. :
Flow Cytometry, IHC, IP, Western Blotting
Cat # :
108101
Clone :
D7
Item :
Purified anti-mouse Ly-6A/E (Sca-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sca-1
Size :
50 μg
Price (USD) :
50 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
IL-2-dependent mouse T-cell line (CTL-L)
Application Notes :
The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting 1,2 , immunoprecipitation 1 , in vitro lymphocyte activation 3-6 , induction of redirected lysis 7 , induction of T cell inhibitory signalling 8 , immunofluorescence 9 , and immunohistochemical staining of acetone-fixed frozen sections 13 and Bouin-fixed, paraffin-embedded samples 9 . The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7. 14
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA