Spark Blue™ 550 anti-mouse I-A/I-E | BioLegend 107662 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Spark Blue™ 550 anti-mouse I-A/I-E

catalog :

107662

quantity :

100 μg

price :

375 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

M5/114.15.2

reactivity :

human, mouse

application :

immunohistochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; fig 4d	Tran C, Scurr M, O Connor L, Buzzelli J, Ng G, Chin S, et al. IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells. Oncotarget. 2022;13:785-799 pubmed publisher
flow cytometry; mouse; 1:100	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig e5f	Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig s3b	Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
flow cytometry; mouse; fig 5a	Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
flow cytometry; human; loading ...; fig 3b	Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
flow cytometry; mouse; loading ...; fig 1f, s2b	Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 3c	Sorrelle N, Ganguly D, Dominguez A, Zhang Y, Huang H, Dahal L, et al. Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues. J Immunol. 2019;202:292-299 pubmed publisher
	Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
	Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
	Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
	Postat J, Olekhnovitch R, Lemaitre F, Bousso P. A Metabolism-Based Quorum Sensing Mechanism Contributes to Termination of Inflammatory Responses. Immunity. 2018;49:654-665.e5 pubmed publisher

product information

Antigen :

I-A/I-E

Apps. Abbrev. :

Cat # :

107662

Clone :

M5/114.15.2

Item :

Spark Blue™ 550 anti-mouse I-A/I-E

Isotype :

Rat IgG2b, κ

Other Names :

MHC class II

Size :

100 μg

Price (USD) :

375 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

SPKBL_550

Immunogen :

Activated C57BL/6 mouse spleen cells

Application Notes :

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens from mice carrying H-2^p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A^f, I-A^k, or I-A^s MHC class II alloantigens.^1Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemistry of frozen sections^2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding^4-7, and spatial biology (IBEX)^17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).;

more info or order :

BioLegend product webpage