product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse I-A/I-E
catalog :
107655
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
M5/114.15.2
reactivity :
human, mouse
application :
immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; fig 4d
Tran C, Scurr M, O Connor L, Buzzelli J, Ng G, Chin S, et al. IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells. Oncotarget. 2022;13:785-799 pubmed publisher
  • flow cytometry; mouse; 1:100
Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e5f
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s3b
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; fig 5a
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1d
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f, s2b
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3c
Sorrelle N, Ganguly D, Dominguez A, Zhang Y, Huang H, Dahal L, et al. Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues. J Immunol. 2019;202:292-299 pubmed publisher
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
Baraibar I, Roman M, Rodriguez Remirez M, Lopez I, Vilalta A, Guruceaga E, et al. Id1 and PD-1 Combined Blockade Impairs Tumor Growth and Survival of KRAS-mutant Lung Cancer by Stimulating PD-L1 Expression and Tumor Infiltrating CD8+ T Cells. Cancers (Basel). 2020;12: pubmed publisher
Humeau J, Sauvat A, Cerrato G, Xie W, Loos F, Iannantuoni F, et al. Inhibition of transcription by dactinomycin reveals a new characteristic of immunogenic cell stress. EMBO Mol Med. 2020;12:e11622 pubmed publisher
Santana Magal N, Farhat Younis L, Gutwillig A, Gleiberman A, Rasoulouniriana D, Tal L, et al. Melanoma-Secreted Lysosomes Trigger Monocyte-Derived Dendritic Cell Apoptosis and Limit Cancer Immunotherapy. Cancer Res. 2020;80:1942-1956 pubmed publisher
Clark D, Brazina S, Yang F, Hu D, Hsieh C, Niemi E, et al. Age-related changes to macrophages are detrimental to fracture healing in mice. Aging Cell. 2020;19:e13112 pubmed publisher
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
Williams J, Li S, Higgs E, Cabanov A, Wang X, Huang H, et al. Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells. Nat Commun. 2020;11:602 pubmed publisher
Hudson W, Gensheimer J, Hashimoto M, Wieland A, Valanparambil R, Li P, et al. Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1+ Stem-like CD8+ T Cells during Chronic Infection. Immunity. 2019;51:1043-1058.e4 pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
Simmons S, Sasaki N, Umemoto E, Uchida Y, Fukuhara S, Kitazawa Y, et al. High-endothelial cell-derived S1P regulates dendritic cell localization and vascular integrity in the lymph node. elife. 2019;8: pubmed publisher
Lecocq Q, Zeven K, De Vlaeminck Y, Martens S, Massa S, Goyvaerts C, et al. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules. 2019;9: pubmed publisher
Zhu W, Naler L, Maul R, Sallin M, Sen J. Immune system development and age-dependent maintenance in Klotho-hypomorphic mice. Aging (Albany NY). 2019;11:5246-5257 pubmed publisher
Ou P, Wen L, Liu X, HUANG J, Huang X, Su C, et al. Thioesterase PPT1 balances viral resistance and efficient T cell crosspriming in dendritic cells. J Exp Med. 2019;: pubmed publisher
Loh J, Xu S, Huo J, Kim S, Wang Y, Lam K. Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity. J Clin Invest. 2019;129:2717-2729 pubmed publisher
Hornick E, Dagvadorj J, Zacharias Z, Miller A, Langlois R, Chen P, et al. Dendritic cell NLRC4 regulates influenza A virus-specific CD4 T cell responses through FasL expression. J Clin Invest. 2019;129:2888-2897 pubmed publisher
Uderhardt S, Martins A, Tsang J, Lämmermann T, Germain R. Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 2019;177:541-555.e17 pubmed publisher
Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, et al. Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5-/- mice. Nat Commun. 2019;10:1492 pubmed publisher
Codina A, Renauer P, Wang G, Chow R, Park J, Ye H, et al. Convergent Identification and Interrogation of Tumor-Intrinsic Factors that Modulate Cancer Immunity In Vivo. Cell Syst. 2019;8:136-151.e7 pubmed publisher
Kubli S, Bassi C, Roux C, Wakeham A, Göbl C, Zhou W, et al. AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer. Proc Natl Acad Sci U S A. 2019;116:3604-3613 pubmed publisher
Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
Turula H, Bragazzi Cunha J, Mainou B, Ramakrishnan S, Wilke C, Gonzalez Hernandez M, et al. Natural Secretory Immunoglobulins Promote Enteric Viral Infections. J Virol. 2018;92: pubmed publisher
Williams G, Schonhoff A, Jurkuvenaite A, Thome A, Standaert D, Harms A. Targeting of the class II transactivator attenuates inflammation and neurodegeneration in an alpha-synuclein model of Parkinson's disease. J Neuroinflammation. 2018;15:244 pubmed publisher
Hirakawa M, Nagakubo D, Kanzler B, Avilov S, Krauth B, Happe C, et al. Fundamental parameters of the developing thymic epithelium in the mouse. Sci Rep. 2018;8:11095 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Li R, Liu J, Wu S, Zai X, Li Y, Yang Q, et al. Toll-like receptor 4 signalling regulates antibody response to adenoviral vector-based vaccines by imprinting germinal centre quality. Immunology. 2018;155:251-262 pubmed publisher
Luo N, Formisano L, Gonzalez Ericsson P, Sanchez V, Dean P, Opalenik S, et al. Melanoma response to anti-PD-L1 immunotherapy requires JAK1 signaling, but not JAK2. Oncoimmunology. 2018;7:e1438106 pubmed publisher
Lau A, Chung H, Komada T, Platnich J, Sandall C, Choudhury S, et al. Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury. J Clin Invest. 2018;128:2894-2913 pubmed publisher
Wein A, Dunbar P, McMaster S, Li Z, Denning T, Kohlmeier J. IL-36γ Protects against Severe Influenza Infection by Promoting Lung Alveolar Macrophage Survival and Limiting Viral Replication. J Immunol. 2018;201:573-582 pubmed publisher
Seydoux E, Liang H, Dubois Cauwelaert N, Archer M, Rintala N, Kramer R, et al. Effective Combination Adjuvants Engage Both TLR and Inflammasome Pathways To Promote Potent Adaptive Immune Responses. J Immunol. 2018;201:98-112 pubmed publisher
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
Califano D, Furuya Y, Metzger D. Effects of Influenza on Alveolar Macrophage Viability Are Dependent on Mouse Genetic Strain. J Immunol. 2018;201:134-144 pubmed publisher
Paschalis E, Lei F, Zhou C, Kapoulea V, Thanos A, Dana R, et al. The Role of Microglia and Peripheral Monocytes in Retinal Damage after Corneal Chemical Injury. Am J Pathol. 2018;188:1580-1596 pubmed publisher
Panduro M, Benoist C, Mathis D. Treg cells limit IFN-? production to control macrophage accrual and phenotype during skeletal muscle regeneration. Proc Natl Acad Sci U S A. 2018;115:E2585-E2593 pubmed publisher
King E, Mazor R, Cuburu N, Pastan I. Low-Dose Methotrexate Prevents Primary and Secondary Humoral Immune Responses and Induces Immune Tolerance to a Recombinant Immunotoxin. J Immunol. 2018;200:2038-2045 pubmed publisher
Wheeler D, Sariol A, Meyerholz D, Perlman S. Microglia are required for protection against lethal coronavirus encephalitis in mice. J Clin Invest. 2018;128:931-943 pubmed publisher
Qing X, Chinenov Y, Redecha P, Madaio M, Roelofs J, FARBER G, et al. iRhom2 promotes lupus nephritis through TNF-? and EGFR signaling. J Clin Invest. 2018;128:1397-1412 pubmed publisher
Kilgore A, Welsh S, Cheney E, Chitrakar A, Blain T, Kedl B, et al. IL-27p28 Production by XCR1+ Dendritic Cells and Monocytes Effectively Predicts Adjuvant-Elicited CD8+ T Cell Responses. Immunohorizons. 2018;2:1-11 pubmed publisher
Mazor R, King E, Onda M, Cuburu N, Addissie S, Crown D, et al. Tolerogenic nanoparticles restore the antitumor activity of recombinant immunotoxins by mitigating immunogenicity. Proc Natl Acad Sci U S A. 2018;115:E733-E742 pubmed publisher
Choi I, Wang Z, Ke Q, Hong M, Qian Y, Zhao X, et al. Signaling by the Epstein-Barr virus LMP1 protein induces potent cytotoxic CD4+ and CD8+ T cell responses. Proc Natl Acad Sci U S A. 2018;115:E686-E695 pubmed publisher
Hornick E, Banoth B, Miller A, Zacharias Z, Jain N, Wilson M, et al. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection. J Immunol. 2018;200:1188-1197 pubmed publisher
Xenakis J, Howard E, Smith K, Olbrich C, Huang Y, Anketell D, et al. Resident intestinal eosinophils constitutively express antigen presentation markers and include two phenotypically distinct subsets of eosinophils. Immunology. 2018;154:298-308 pubmed publisher
Harms A, Thome A, Yan Z, Schonhoff A, Williams G, Li X, et al. Peripheral monocyte entry is required for alpha-Synuclein induced inflammation and Neurodegeneration in a model of Parkinson disease. Exp Neurol. 2018;300:179-187 pubmed publisher
Roussey J, Viglianti S, Teitz Tennenbaum S, Olszewski M, Osterholzer J. Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. J Immunol. 2017;199:3535-3546 pubmed publisher
Jain R, Sheridan J, Policheni A, Heinlein M, Gandolfo L, Dewson G, et al. A critical epithelial survival axis regulated by MCL-1 maintains thymic function in mice. Blood. 2017;130:2504-2515 pubmed publisher
Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
Okamoto N, Mizote K, Honda H, Saeki A, Watanabe Y, Yamaguchi Miyamoto T, et al. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex. J Biol Chem. 2017;292:15378-15394 pubmed publisher
Hope C, Emmerich P, Papadas A, Pagenkopf A, Matkowskyj K, Van De Hey D, et al. Versican-Derived Matrikines Regulate Batf3-Dendritic Cell Differentiation and Promote T Cell Infiltration in Colorectal Cancer. J Immunol. 2017;199:1933-1941 pubmed publisher
Nirschl C, Suárez Fariñas M, Izar B, Prakadan S, Dannenfelser R, Tirosh I, et al. IFNγ-Dependent Tissue-Immune Homeostasis Is Co-opted in the Tumor Microenvironment. Cell. 2017;170:127-141.e15 pubmed publisher
Wu S, Niknafs Y, Kim S, Oravecz Wilson K, Zajac C, Toubai T, et al. A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation. Cell Rep. 2017;19:2645-2656 pubmed publisher
Gibb D, Liu J, Natarajan P, Santhanakrishnan M, Madrid D, Eisenbarth S, et al. Type I IFN Is Necessary and Sufficient for Inflammation-Induced Red Blood Cell Alloimmunization in Mice. J Immunol. 2017;199:1041-1050 pubmed publisher
Parashar K, Kopping E, Frank D, Sampath V, Thanassi D, Carpino N. Increased Resistance to Intradermal Francisella tularensis LVS Infection by Inactivation of the Sts Phosphatases. Infect Immun. 2017;85: pubmed publisher
Anderson D, Grajales Reyes G, Satpathy A, Vasquez Hueichucura C, Murphy T, Murphy K. Revisiting the specificity of the MHC class?II transactivator CIITA in classical murine dendritic cells in vivo. Eur J Immunol. 2017;47:1317-1323 pubmed publisher
Dudakov J, Mertelsmann A, O Connor M, Jenq R, Velardi E, Young L, et al. Loss of thymic innate lymphoid cells leads to impaired thymopoiesis in experimental graft-versus-host disease. Blood. 2017;130:933-942 pubmed publisher
Matte Martone C, Liu J, Zhou M, Chikina M, Green D, Harty J, et al. Differential requirements for myeloid leukemia IFN-γ conditioning determine graft-versus-leukemia resistance and sensitivity. J Clin Invest. 2017;127:2765-2776 pubmed publisher
Seki S, Stevenson M, Rosen A, Arandjelovic S, Gemta L, Bullock T, et al. Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System. J Immunol. 2017;198:4607-4617 pubmed publisher
Nagata M, Izumi Y, Ishikawa E, Kiyotake R, Doi R, Iwai S, et al. Intracellular metabolite ?-glucosylceramide is an endogenous Mincle ligand possessing immunostimulatory activity. Proc Natl Acad Sci U S A. 2017;114:E3285-E3294 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Zhang H, Zheng L, McGovern D, Hamill A, Ichikawa R, Kanazawa Y, et al. Myeloid ATG16L1 Facilitates Host-Bacteria Interactions in Maintaining Intestinal Homeostasis. J Immunol. 2017;198:2133-2146 pubmed publisher
Ting H, Schaller M, De Almeida Nagata D, Rasky A, Maillard I, Lukacs N. Notch Ligand Delta-like 4 Promotes Regulatory T Cell Identity in Pulmonary Viral Infection. J Immunol. 2017;198:1492-1502 pubmed publisher
Hey Y, Quah B, O Neill H. Antigen presenting capacity of murine splenic myeloid cells. BMC Immunol. 2017;18:4 pubmed publisher
Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
Sanderson N, Zimmermann M, Eilinger L, Gubser C, Schaeren Wiemers N, Lindberg R, et al. Cocapture of cognate and bystander antigens can activate autoreactive B cells. Proc Natl Acad Sci U S A. 2017;114:734-739 pubmed publisher
Nassar M, Tabib Y, Capucha T, Mizraji G, Nir T, Pevsner Fischer M, et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A. 2017;114:E337-E346 pubmed publisher
product information
Antigen :
I-A/I-E
Apps. Abbrev. :
Flow Cytometry, IHC-F, IP, Block
Cat # :
107655
Clone :
M5/114.15.2
Item :
Ultra-LEAF™ Purified anti-mouse I-A/I-E
Isotype :
Rat IgG2b, κ
Other Names :
MHC class II
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
Activated C57BL/6 mouse spleen cells
Application Notes :
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A b , I-A d , I-A q , I-E d , and I-E k MHC class II alloantigens from mice carrying H-2 p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A f , I-A k , or I-A s MHC class II alloantigens. 1 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemistry of frozen sections 2,3,6 , in vitro and in vivo blocking of antigen presentation or ligand binding 4-7 , and spatial biology (IBEX) 17,18 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 107655 107656).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA