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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse I-A/I-E
catalog :
107627
quantity :
25 μg
price :
85 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
M5/114.15.2
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 1b
Kogo H, Shimizu M, Negishi Y, Uchida E, Takahashi H. Suppression of murine tumour growth through CD8+ cytotoxic T lymphocytes via activated DEC-205+ dendritic cells by sequential administration of ?-galactosylceramide in vivo. Immunology. 2017;151:324-339 pubmed publisher
  • flow cytometry; mouse; tbl s3
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • flow cytometry; mouse
Tassi I, Claudio E, Wang H, Tang W, Ha H, Saret S, et al. The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity. J Immunol. 2014;193:4303-11 pubmed publisher
Maniati E, Berlato C, Gopinathan G, Heath O, Kotantaki P, Lakhani A, et al. Mouse Ovarian Cancer Models Recapitulate the Human Tumor Microenvironment and Patient Response to Treatment. Cell Rep. 2020;30:525-540.e7 pubmed publisher
Hayashida E, Ling Z, Ashhurst T, Viengkhou B, Jung S, Songkhunawej P, et al. Zika virus encephalitis in immunocompetent mice is dominated by innate immune cells and does not require T or B cells. J Neuroinflammation. 2019;16:177 pubmed publisher
Acker K, Wong Fok Lung T, West E, Craft J, Narechania A, Smith H, et al. Strains of Staphylococcus aureus that Colonize and Infect Skin Harbor Mutations in Metabolic Genes. iScience. 2019;19:281-290 pubmed publisher
Liu C, Chikina M, Deshpande R, Menk A, Wang T, Tabib T, et al. Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8+ T Cell-Derived Interferon-γ. Immunity. 2019;51:381-397.e6 pubmed publisher
Kitaura F, Yuno M, Fujita T, Wakana S, Ueda J, Yamagata K, et al. Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice. PLoS ONE. 2019;14:e0220199 pubmed publisher
Deniset J, Belke D, Lee W, Jorch S, Deppermann C, Hassanabad A, et al. Gata6+ Pericardial Cavity Macrophages Relocate to the Injured Heart and Prevent Cardiac Fibrosis. Immunity. 2019;51:131-140.e5 pubmed publisher
Garber C, Soung A, Vollmer L, Kanmogne M, Last A, Brown J, et al. T cells promote microglia-mediated synaptic elimination and cognitive dysfunction during recovery from neuropathogenic flaviviruses. Nat Neurosci. 2019;: pubmed publisher
Li J, Ma C, Long F, Yang D, Liu X, Hu Y, et al. Parkin Impairs Antiviral Immunity by Suppressing the Mitochondrial Reactive Oxygen Species-Nlrp3 Axis and Antiviral Inflammation. iScience. 2019;16:468-484 pubmed publisher
Lancaster J, Thyagarajan H, Srinivasan J, Li Y, Hu Z, Ehrlich L. Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance. Nat Commun. 2019;10:2220 pubmed publisher
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
Smith H, McCaw T, Londoño A, Katre A, Meza Perez S, Yang E, et al. The antitumor effects of entinostat in ovarian cancer require adaptive immunity. Cancer. 2018;124:4657-4666 pubmed publisher
Ubellacker J, Baryawno N, Severe N, Decristo M, Sceneay J, Hutchinson J, et al. Modulating Bone Marrow Hematopoietic Lineage Potential to Prevent Bone Metastasis in Breast Cancer. Cancer Res. 2018;78:5300-5314 pubmed publisher
Bhattacharya A, Lord B, Grigoleit J, He Y, Fraser I, Campbell S, et al. Neuropsychopharmacology of JNJ-55308942: evaluation of a clinical candidate targeting P2X7 ion channels in animal models of neuroinflammation and anhedonia. Neuropsychopharmacology. 2018;43:2586-2596 pubmed publisher
Bellelli R, Borel V, Logan C, Svendsen J, Cox D, Nye E, et al. Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell. 2018;70:707-721.e7 pubmed publisher
An J, Nagaishi T, Watabe T, Naruse T, Watanabe M, Kimura A. MKL1 expressed in macrophages contributes to the development of murine colitis. Sci Rep. 2017;7:13650 pubmed publisher
Gundra U, Girgis N, Gonzalez M, San Tang M, Van Der Zande H, Lin J, et al. Vitamin A mediates conversion of monocyte-derived macrophages into tissue-resident macrophages during alternative activation. Nat Immunol. 2017;18:642-653 pubmed publisher
Chandra J, Kuo P, Hahn A, Belz G, Frazer I. Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function. Immunol Cell Biol. 2017;95:215-223 pubmed publisher
Zysset D, Weber B, Rihs S, Brasseit J, Freigang S, Riether C, et al. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nat Commun. 2016;7:13151 pubmed publisher
Hilpert C, Sitte S, Matthies A, Voehringer D. Dendritic Cells Are Dispensable for T Cell Priming and Control of Acute Lymphocytic Choriomeningitis Virus Infection. J Immunol. 2016;197:2780-6 pubmed publisher
El Ghazal R, Yin X, Johns S, Swanson L, Macal M, Ghosh P, et al. Glycan Sulfation Modulates Dendritic Cell Biology and Tumor Growth. Neoplasia. 2016;18:294-306 pubmed publisher
Lim S, Lee J, Koo J, Kang T, Ha S, Choi J. Cell Type Preference of a Novel Human Derived Cell-Permeable Peptide dNP2 and TAT in Murine Splenic Immune Cells. PLoS ONE. 2016;11:e0155689 pubmed publisher
Engel O, Akyüz L, da Costa Goncalves A, Winek K, Dames C, Thielke M, et al. Cholinergic Pathway Suppresses Pulmonary Innate Immunity Facilitating Pneumonia After Stroke. Stroke. 2015;46:3232-40 pubmed publisher
Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration. PLoS ONE. 2015;10:e0136620 pubmed publisher
Schwartz C, Turqueti Neves A, Hartmann S, Yu P, Nimmerjahn F, Voehringer D. Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion. Proc Natl Acad Sci U S A. 2014;111:E5169-77 pubmed publisher
Céspedes P, Bueno S, Ramírez B, Gómez R, Riquelme S, Palavecino C, et al. Surface expression of the hRSV nucleoprotein impairs immunological synapse formation with T cells. Proc Natl Acad Sci U S A. 2014;111:E3214-23 pubmed publisher
Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, et al. Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE. J Neurosci. 2014;34:8175-85 pubmed publisher
Becerra Díaz M, Terrazas L. Taenia crassiceps infection and its excreted/secreted products inhibit STAT1 activation in response to IFN-?. Int J Parasitol. 2014;44:613-23 pubmed publisher
Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
Dráber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, et al. SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011;31:4550-62 pubmed publisher
Sabatino J, Huang J, Zhu C, Evavold B. High prevalence of low affinity peptide-MHC II tetramer-negative effectors during polyclonal CD4+ T cell responses. J Exp Med. 2011;208:81-90 pubmed publisher
Henningsson L, Jirholt P, Lindholm C, Eneljung T, Silverpil E, Iwakura Y, et al. Interleukin-17A during local and systemic Staphylococcus aureus-induced arthritis in mice. Infect Immun. 2010;78:3783-90 pubmed publisher
Kuns R, Morris E, MacDonald K, Markey K, Morris H, Raffelt N, et al. Invariant natural killer T cell-natural killer cell interactions dictate transplantation outcome after alpha-galactosylceramide administration. Blood. 2009;113:5999-6010 pubmed publisher
De Pascalis R, Taylor B, Elkins K. Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain. Infect Immun. 2008;76:4311-21 pubmed publisher
Matte Martone C, Liu J, Jain D, McNiff J, Shlomchik W. CD8+ but not CD4+ T cells require cognate interactions with target tissues to mediate GVHD across only minor H antigens, whereas both CD4+ and CD8+ T cells require direct leukemic contact to mediate GVL. Blood. 2008;111:3884-92 pubmed publisher
Christensen S, Shupe J, Nickerson K, Kashgarian M, Flavell R, Shlomchik M. Toll-like receptor 7 and TLR9 dictate autoantibody specificity and have opposing inflammatory and regulatory roles in a murine model of lupus. Immunity. 2006;25:417-28 pubmed
product information
Antigen :
I-A/I-E
Apps. Abbrev. :
Flow Cytometry
Cat # :
107627
Clone :
M5/114.15.2
Item :
APC/Cyanine7 anti-mouse I-A/I-E
Isotype :
Rat IgG2b, κ
Other Names :
MHC class II
Size :
25 μg
Price (USD) :
85 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Activated C57BL/6 mouse spleen cells
Application Notes :
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A b , I-A d , I-A q , I-E d , and I-E k MHC class II alloantigens from mice carrying H-2 p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A f , I-A k , or I-A s MHC class II alloantigens. 1 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemistry of frozen sections 2,3,6 , and in vitro and in vivo blocking of antigen presentation or ligand binding 4-7 . The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107655 107656).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA