product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse I-A/I-E
catalog :
107613
quantity :
25 μg
price :
66 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
M5/114.15.2
reactivity :
mouse
application :
immunohistochemistry, flow cytometry
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 4a
Van Maldegem F, Valand K, Cole M, Patel H, Angelova M, Rana S, et al. Characterisation of tumour microenvironment remodelling following oncogene inhibition in preclinical studies with imaging mass cytometry. Nat Commun. 2021;12:5906 pubmed publisher
  • flow cytometry; mouse
Muller A, Dickmanns A, Resch C, Schakel K, Hailfinger S, Dobbelstein M, et al. The CDK4/6-EZH2 pathway is a potential therapeutic target for psoriasis. J Clin Invest. 2020;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3
Grist J, Marro B, Skinner D, Syage A, Worne C, Doty D, et al. Induced CNS expression of CXCL1 augments neurologic disease in a murine model of multiple sclerosis via enhanced neutrophil recruitment. Eur J Immunol. 2018;48:1199-1210 pubmed publisher
  • flow cytometry; mouse; fig 2
Lieber S, Scheer F, Finkernagel F, Meissner W, Giehl G, Brendel C, et al. The inverse agonist DG172 triggers a PPARβ/δ-independent myeloid lineage shift and promotes GM-CSF/IL-4-induced dendritic cell differentiation. Mol Pharmacol. 2015;87:162-73 pubmed publisher
Wang R, Chen J, Wang W, Zhao Z, Wang H, Liu S, et al. CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model. J Immunother Cancer. 2022;10: pubmed publisher
Zhao H, Wang M, Gao Y, Wu X, Xiao H, Yang D, et al. Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice. Commun Biol. 2022;5:74 pubmed publisher
Jorapur A, Marshall L, Jacobson S, Xu M, Marubayashi S, Zibinsky M, et al. EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22. PLoS Pathog. 2022;18:e1010200 pubmed publisher
Kiritsy M, McCann K, MOTT D, Holland S, Behar S, Sassetti C, et al. Mitochondrial respiration contributes to the interferon gamma response in antigen-presenting cells. elife. 2021;10: pubmed publisher
Olson G, Murray T, Jahn A, Mai D, Diercks A, Gold E, et al. Type I interferon decreases macrophage energy metabolism during mycobacterial infection. Cell Rep. 2021;35:109195 pubmed publisher
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, et al. PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction. Cell Rep. 2020;33:108278 pubmed publisher
Lucas E, Schafer J, Matsuda J, Kraus M, Burchill M, Tamburini B. PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity. Cell Rep. 2020;33:108258 pubmed publisher
Rothchild A, Mai D, Aderem A, Diercks A. Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice. Bio Protoc. 2020;10: pubmed publisher
Liang J, Wang H, Ding W, Huang J, Zhou X, Wang H, et al. Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity. Sci Adv. 2020;6:eabc3646 pubmed publisher
Lv Z, Zhang P, Li D, Qin M, Nie L, Wang X, et al. CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models. Oncoimmunology. 2020;9:1747688 pubmed publisher
Dheilly E, Battistello E, Katanayeva N, Sungalee S, Michaux J, Duns G, et al. Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma. Cancer Cell. 2020;37:674-689.e12 pubmed publisher
Garcia Fabiani M, Comba A, Kadiyala P, Haase S, Núñez F, Altshuler D, et al. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system. Methods Enzymol. 2020;632:369-388 pubmed publisher
Aegerter H, Kulikauskaite J, Crotta S, Patel H, Kelly G, Hessel E, et al. Influenza-induced monocyte-derived alveolar macrophages confer prolonged antibacterial protection. Nat Immunol. 2020;21:145-157 pubmed publisher
Liu Y, Qi X, Li G, Sowa G. Caveolin-2 deficiency induces a rapid anti-tumor immune response prior to regression of implanted murine lung carcinoma tumors. Sci Rep. 2019;9:18970 pubmed publisher
Levesque S, Le Naour J, Pietrocola F, Paillet J, Kremer M, Castoldi F, et al. A synergistic triad of chemotherapy, immune checkpoint inhibitors, and caloric restriction mimetics eradicates tumors in mice. Oncoimmunology. 2019;8:e1657375 pubmed publisher
Deniset J, Belke D, Lee W, Jorch S, Deppermann C, Hassanabad A, et al. Gata6+ Pericardial Cavity Macrophages Relocate to the Injured Heart and Prevent Cardiac Fibrosis. Immunity. 2019;51:131-140.e5 pubmed publisher
Omenetti S, Bussi C, Metidji A, Iseppon A, Lee S, Tolaini M, et al. The Intestine Harbors Functionally Distinct Homeostatic Tissue-Resident and Inflammatory Th17 Cells. Immunity. 2019;: pubmed publisher
Zhou J, Huang S, Wang Z, Huang J, Xu L, Tang X, et al. Targeting EZH2 histone methyltransferase activity alleviates experimental intestinal inflammation. Nat Commun. 2019;10:2427 pubmed publisher
Kurup S, Anthony S, Hancox L, Vijay R, Pewe L, Moioffer S, et al. Monocyte-Derived CD11c+ Cells Acquire Plasmodium from Hepatocytes to Prime CD8 T Cell Immunity to Liver-Stage Malaria. Cell Host Microbe. 2019;: pubmed publisher
Kobayashi T, Voisin B, Kim D, Kennedy E, Jo J, Shih H, et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell. 2019;176:982-997.e16 pubmed publisher
Zhu W, Xu R, Du J, Fu Y, Li S, Zhang P, et al. Zoledronic acid promotes TLR-4-mediated M1 macrophage polarization in bisphosphonate-related osteonecrosis of the jaw. FASEB J. 2019;33:5208-5219 pubmed publisher
Ying W, Lee Y, Dong Y, Seidman J, Yang M, Isaac R, et al. Expansion of Islet-Resident Macrophages Leads to Inflammation Affecting β Cell Proliferation and Function in Obesity. Cell Metab. 2019;29:457-474.e5 pubmed publisher
Al Mamun A, Yu H, Mirza M, Romana S, McCullough L, Liu F. Myeloid cell IRF4 signaling protects neonatal brains from hypoxic ischemic encephalopathy. Neurochem Int. 2019;127:148-157 pubmed publisher
Chen S, Fang L, Guo W, Zhou Y, Yu G, Li W, et al. Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells. Nat Commun. 2018;9:5298 pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Van Winkle J, Robinson B, Peters A, Li L, Nouboussi R, Mack M, et al. Persistence of Systemic Murine Norovirus Is Maintained by Inflammatory Recruitment of Susceptible Myeloid Cells. Cell Host Microbe. 2018;24:665-676.e4 pubmed publisher
Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, et al. Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Cell. 2018;175:1336-1351.e17 pubmed publisher
Hou Y, Liang H, Rao E, Zheng W, Huang X, Deng L, et al. Non-canonical NF-κB Antagonizes STING Sensor-Mediated DNA Sensing in Radiotherapy. Immunity. 2018;49:490-503.e4 pubmed publisher
Combes F, Mc Cafferty S, Meyer E, Sanders N. Off-Target and Tumor-Specific Accumulation of Monocytes, Macrophages and Myeloid-Derived Suppressor Cells after Systemic Injection. Neoplasia. 2018;20:848-856 pubmed publisher
Si Y, Wen Y, Kelly S, Chong A, Collier J. Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8+ T cell responses. J Control Release. 2018;282:120-130 pubmed publisher
Best S, De Souza D, Kersbergen A, Policheni A, Dayalan S, Tull D, et al. Synergy between the KEAP1/NRF2 and PI3K Pathways Drives Non-Small-Cell Lung Cancer with an Altered Immune Microenvironment. Cell Metab. 2018;27:935-943.e4 pubmed publisher
Arima Y, Ohki T, Nishikawa N, Higuchi K, Ota M, Tanaka Y, et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. elife. 2017;6: pubmed publisher
Mattila J, Beaino W, Maiello P, Coleman M, White A, Scanga C, et al. Positron Emission Tomography Imaging of Macaques with Tuberculosis Identifies Temporal Changes in Granuloma Glucose Metabolism and Integrin ?4?1-Expressing Immune Cells. J Immunol. 2017;199:806-815 pubmed publisher
Bansal K, Yoshida H, Benoist C, Mathis D. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol. 2017;18:263-273 pubmed publisher
Morikawa M, Tsujibe S, Kiyoshima Shibata J, Watanabe Y, Kato Nagaoka N, Shida K, et al. Microbiota of the Small Intestine Is Selectively Engulfed by Phagocytes of the Lamina Propria and Peyer's Patches. PLoS ONE. 2016;11:e0163607 pubmed publisher
Kitur K, Wachtel S, Brown A, Wickersham M, Paulino F, Peñaloza H, et al. Necroptosis Promotes Staphylococcus aureus Clearance by Inhibiting Excessive Inflammatory Signaling. Cell Rep. 2016;16:2219-2230 pubmed publisher
Boucard Jourdin M, Kugler D, Endale Ahanda M, This S, De Calisto J, Zhang A, et al. ?8 Integrin Expression and Activation of TGF-? by Intestinal Dendritic Cells Are Determined by Both Tissue Microenvironment and Cell Lineage. J Immunol. 2016;197:1968-78 pubmed publisher
Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
Kitano M, Yamazaki C, Takumi A, Ikeno T, Hemmi H, Takahashi N, et al. Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node. Proc Natl Acad Sci U S A. 2016;113:1044-9 pubmed publisher
Meunier I, Morisseau O, Garneau E, Marois I, Cloutier A, Richter M. Infection with a Mouse-Adapted Strain of the 2009 Pandemic Virus Causes a Highly Severe Disease Associated with an Impaired T Cell Response. PLoS ONE. 2015;10:e0138055 pubmed publisher
Kitur K, Parker D, Nieto P, Ahn D, Cohen T, Chung S, et al. Toxin-induced necroptosis is a major mechanism of Staphylococcus aureus lung damage. PLoS Pathog. 2015;11:e1004820 pubmed publisher
Lee M, Shim D, Yoon J, Jang H, Oh S, Suh S, et al. Retnla overexpression attenuates allergic inflammation of the airway. PLoS ONE. 2014;9:e112666 pubmed publisher
Tassi I, Claudio E, Wang H, Tang W, Ha H, Saret S, et al. The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity. J Immunol. 2014;193:4303-11 pubmed publisher
Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
Dráber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, et al. SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011;31:4550-62 pubmed publisher
Sabatino J, Huang J, Zhu C, Evavold B. High prevalence of low affinity peptide-MHC II tetramer-negative effectors during polyclonal CD4+ T cell responses. J Exp Med. 2011;208:81-90 pubmed publisher
Rayamajhi M, Humann J, Penheiter K, Andreasen K, Lenz L. Induction of IFN-alphabeta enables Listeria monocytogenes to suppress macrophage activation by IFN-gamma. J Exp Med. 2010;207:327-37 pubmed publisher
Kuns R, Morris E, MacDonald K, Markey K, Morris H, Raffelt N, et al. Invariant natural killer T cell-natural killer cell interactions dictate transplantation outcome after alpha-galactosylceramide administration. Blood. 2009;113:5999-6010 pubmed publisher
De Pascalis R, Taylor B, Elkins K. Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain. Infect Immun. 2008;76:4311-21 pubmed publisher
Matte Martone C, Liu J, Jain D, McNiff J, Shlomchik W. CD8+ but not CD4+ T cells require cognate interactions with target tissues to mediate GVHD across only minor H antigens, whereas both CD4+ and CD8+ T cells require direct leukemic contact to mediate GVL. Blood. 2008;111:3884-92 pubmed publisher
Gray D, Abramson J, Benoist C, Mathis D. Proliferative arrest and rapid turnover of thymic epithelial cells expressing Aire. J Exp Med. 2007;204:2521-8 pubmed
Christensen S, Shupe J, Nickerson K, Kashgarian M, Flavell R, Shlomchik M. Toll-like receptor 7 and TLR9 dictate autoantibody specificity and have opposing inflammatory and regulatory roles in a murine model of lupus. Immunity. 2006;25:417-28 pubmed
product information
Antigen :
I-A/I-E
Apps. Abbrev. :
Flow Cytometry
Cat # :
107613
Clone :
M5/114.15.2
Item :
APC anti-mouse I-A/I-E
Isotype :
Rat IgG2b, κ
Other Names :
MHC class II
Size :
25 μg
Price (USD) :
66 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
Activated C57BL/6 mouse spleen cells
Application Notes :
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A b , I-A d , I-A q , I-E d , and I-E k MHC class II alloantigens from mice carrying H-2 p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A f , I-A k , or I-A s MHC class II alloantigens. 1 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemistry of frozen sections 2,3,6 , in vitro and in vivo blocking of antigen presentation or ligand binding 4-7 , and spatial biology (IBEX) 17,18 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 107655 107656).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA